Skip Header

You are using a version of browser that may not display all the features of this website. Please consider upgrading your browser.
Protein

Type 4 prepilin-like proteins leader peptide-processing enzyme

Gene

comC

Organism
Bacillus subtilis (strain 168)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Experimental evidence at transcript leveli

Functioni

Cleaves type-4 fimbrial leader sequence and methylates the N-terminal (generally Phe) residue.By similarity

Catalytic activityi

Typically cleaves a -Gly-|-Phe- bond to release an N-terminal, basic peptide of 5-8 residues from type IV prepilin, and then N-methylates the new N-terminal amino group, the methyl donor being S-adenosyl-L-methionine.

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Hydrolase, Methyltransferase, Protease, Transferase

Keywords - Biological processi

Competence, Sporulation

Keywords - Ligandi

S-adenosyl-L-methionine

Enzyme and pathway databases

BioCyciBSUB:BSU28070-MONOMER.

Protein family/group databases

MEROPSiA24.019.

Names & Taxonomyi

Protein namesi
Recommended name:
Type 4 prepilin-like proteins leader peptide-processing enzyme
Alternative name(s):
Late competence protein ComC
Including the following 2 domains:
Leader peptidase (EC:3.4.23.43)
Alternative name(s):
Prepilin peptidase
N-methyltransferase (EC:2.1.1.-)
Gene namesi
Name:comC
Ordered Locus Names:BSU28070
OrganismiBacillus subtilis (strain 168)
Taxonomic identifieri224308 [NCBI]
Taxonomic lineageiBacteriaFirmicutesBacilliBacillalesBacillaceaeBacillus
Proteomesi
  • UP000001570 Componenti: Chromosome

Subcellular locationi

Topology

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Transmembranei1 – 2121HelicalSequence analysisAdd
BLAST
Transmembranei68 – 8821HelicalSequence analysisAdd
BLAST
Transmembranei90 – 11021HelicalSequence analysisAdd
BLAST
Transmembranei114 – 13421HelicalSequence analysisAdd
BLAST
Transmembranei143 – 16321HelicalSequence analysisAdd
BLAST
Transmembranei178 – 19821HelicalSequence analysisAdd
BLAST
Transmembranei223 – 24321HelicalSequence analysisAdd
BLAST

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Cell membrane, Membrane

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 248248Type 4 prepilin-like proteins leader peptide-processing enzymePRO_0000192616Add
BLAST

Proteomic databases

PaxDbiP15378.

Expressioni

Inductioni

By several proteins including Spo0A, Spo0H, Sin, AbrB, DegS, DegU, ComA, ComB and ComK.

Interactioni

Protein-protein interaction databases

STRINGi224308.Bsubs1_010100015341.

Family & Domainsi

Sequence similaritiesi

Belongs to the peptidase A24 family.Curated

Keywords - Domaini

Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiENOG4106A3R. Bacteria.
ENOG410XV1V. LUCA.
HOGENOMiHOG000248583.
InParanoidiP15378.
KOiK02236.
OMAiRCAYCHE.
OrthoDBiEOG6F55M8.
PhylomeDBiP15378.

Family and domain databases

InterProiIPR010627. Pept_A24A_N.
IPR000045. Prepilin_IV_endopep_pep.
[Graphical view]
PfamiPF06750. DiS_P_DiS. 1 hit.
PF01478. Peptidase_A24. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

P15378-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MLSILFIFGL ILGSFYYTAG CRIPLHLSII APRSSCPFCR RTLTPAELIP
60 70 80 90 100
ILSFLFQKGK CKSCGHRISF MYPAAELVTA CLFAAAGIRF GISLELFPAV
110 120 130 140 150
VFISLLIIVA VTDIHFMLIP NRILIFFLPF LAAARLISPL DSWYAGLLGA
160 170 180 190 200
AAGFLFLAVI AAITHGGVGG GDIKLFAVIG FVLGVKMLAA AFFFSVLIGA
210 220 230 240
LYGAAAVLTG RLAKRQPLPF APAIAAGSIL AYLYGDSIIS FYIKMALG
Length:248
Mass (Da):26,435
Last modified:July 7, 2009 - v2
Checksum:i5266453B73D26097
GO

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti206 – 2061A → R in AAA83365 (PubMed:2553669).Curated
Sequence conflicti206 – 2061A → R in AAB59025 (PubMed:8419299).Curated

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
M30805 Genomic DNA. Translation: AAA83365.1.
AL009126 Genomic DNA. Translation: CAB14767.2.
L04520 Genomic DNA. Translation: AAB59022.1.
L04519 Genomic DNA. Translation: AAB59025.1.
PIRiA33490.
RefSeqiNP_390685.2. NC_000964.3.
WP_010886586.1. NC_000964.3.

Genome annotation databases

EnsemblBacteriaiCAB14767; CAB14767; BSU28070.
GeneIDi936586.
KEGGibsu:BSU28070.
PATRICi18977486. VBIBacSub10457_2932.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
M30805 Genomic DNA. Translation: AAA83365.1.
AL009126 Genomic DNA. Translation: CAB14767.2.
L04520 Genomic DNA. Translation: AAB59022.1.
L04519 Genomic DNA. Translation: AAB59025.1.
PIRiA33490.
RefSeqiNP_390685.2. NC_000964.3.
WP_010886586.1. NC_000964.3.

3D structure databases

ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi224308.Bsubs1_010100015341.

Protein family/group databases

MEROPSiA24.019.

Proteomic databases

PaxDbiP15378.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiCAB14767; CAB14767; BSU28070.
GeneIDi936586.
KEGGibsu:BSU28070.
PATRICi18977486. VBIBacSub10457_2932.

Phylogenomic databases

eggNOGiENOG4106A3R. Bacteria.
ENOG410XV1V. LUCA.
HOGENOMiHOG000248583.
InParanoidiP15378.
KOiK02236.
OMAiRCAYCHE.
OrthoDBiEOG6F55M8.
PhylomeDBiP15378.

Enzyme and pathway databases

BioCyciBSUB:BSU28070-MONOMER.

Family and domain databases

InterProiIPR010627. Pept_A24A_N.
IPR000045. Prepilin_IV_endopep_pep.
[Graphical view]
PfamiPF06750. DiS_P_DiS. 1 hit.
PF01478. Peptidase_A24. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. "Molecular cloning and characterization of comC, a late competence gene of Bacillus subtilis."
    Mohan S., Aghion J., Guillen N., Dubnau D.A.
    J. Bacteriol. 171:6043-6051(1989) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
  2. "The complete genome sequence of the Gram-positive bacterium Bacillus subtilis."
    Kunst F., Ogasawara N., Moszer I., Albertini A.M., Alloni G., Azevedo V., Bertero M.G., Bessieres P., Bolotin A., Borchert S., Borriss R., Boursier L., Brans A., Braun M., Brignell S.C., Bron S., Brouillet S., Bruschi C.V.
    , Caldwell B., Capuano V., Carter N.M., Choi S.-K., Codani J.-J., Connerton I.F., Cummings N.J., Daniel R.A., Denizot F., Devine K.M., Duesterhoeft A., Ehrlich S.D., Emmerson P.T., Entian K.-D., Errington J., Fabret C., Ferrari E., Foulger D., Fritz C., Fujita M., Fujita Y., Fuma S., Galizzi A., Galleron N., Ghim S.-Y., Glaser P., Goffeau A., Golightly E.J., Grandi G., Guiseppi G., Guy B.J., Haga K., Haiech J., Harwood C.R., Henaut A., Hilbert H., Holsappel S., Hosono S., Hullo M.-F., Itaya M., Jones L.-M., Joris B., Karamata D., Kasahara Y., Klaerr-Blanchard M., Klein C., Kobayashi Y., Koetter P., Koningstein G., Krogh S., Kumano M., Kurita K., Lapidus A., Lardinois S., Lauber J., Lazarevic V., Lee S.-M., Levine A., Liu H., Masuda S., Mauel C., Medigue C., Medina N., Mellado R.P., Mizuno M., Moestl D., Nakai S., Noback M., Noone D., O'Reilly M., Ogawa K., Ogiwara A., Oudega B., Park S.-H., Parro V., Pohl T.M., Portetelle D., Porwollik S., Prescott A.M., Presecan E., Pujic P., Purnelle B., Rapoport G., Rey M., Reynolds S., Rieger M., Rivolta C., Rocha E., Roche B., Rose M., Sadaie Y., Sato T., Scanlan E., Schleich S., Schroeter R., Scoffone F., Sekiguchi J., Sekowska A., Seror S.J., Serror P., Shin B.-S., Soldo B., Sorokin A., Tacconi E., Takagi T., Takahashi H., Takemaru K., Takeuchi M., Tamakoshi A., Tanaka T., Terpstra P., Tognoni A., Tosato V., Uchiyama S., Vandenbol M., Vannier F., Vassarotti A., Viari A., Wambutt R., Wedler E., Wedler H., Weitzenegger T., Winters P., Wipat A., Yamamoto H., Yamane K., Yasumoto K., Yata K., Yoshida K., Yoshikawa H.-F., Zumstein E., Yoshikawa H., Danchin A.
    Nature 390:249-256(1997) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: 168.
  3. "From a consortium sequence to a unified sequence: the Bacillus subtilis 168 reference genome a decade later."
    Barbe V., Cruveiller S., Kunst F., Lenoble P., Meurice G., Sekowska A., Vallenet D., Wang T., Moszer I., Medigue C., Danchin A.
    Microbiology 155:1758-1775(2009) [PubMed] [Europe PMC] [Abstract]
    Cited for: SEQUENCE REVISION TO 206.
  4. "Sporulation gene spoIIB from Bacillus subtilis."
    Margolis P.S., Driks A., Losick R.
    J. Bacteriol. 175:528-540(1993) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 1-46 AND 181-248.
    Strain: 168 / PY79.

Entry informationi

Entry nameiLEP4_BACSU
AccessioniPrimary (citable) accession number: P15378
Entry historyi
Integrated into UniProtKB/Swiss-Prot: April 1, 1990
Last sequence update: July 7, 2009
Last modified: February 17, 2016
This is version 121 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Multifunctional enzyme, Reference proteome

Documents

  1. Bacillus subtilis
    Bacillus subtilis (strain 168): entries, gene names and cross-references to SubtiList
  2. Peptidase families
    Classification of peptidase families and list of entries
  3. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.