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Protein

Antitoxin PrlF

Gene

prlF

Organism
Escherichia coli (strain K12)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Antitoxin component of a type II toxin-antitoxin (TA) system. Labile antitoxin that binds to the YhaV toxin and neutralizes its ribonuclease activity. Also acts as a transcription factor. The YhaV/PrlF complex binds the prlF-yhaV operon, probably negatively regulating its expression.1 Publication
Negatively regulates its own expression as well as relieving the export block imposed by high-level synthesis of the LamB-LacZ hybrid protein. Overexpression leads to increased doubling time and also suppresses a htrA (degP) null phenotype.1 Publication

GO - Molecular functioni

  • DNA binding Source: UniProtKB-KW
  • DNA binding transcription factor activity Source: EcoCyc
  • enzyme binding Source: EcoCyc
  • identical protein binding Source: EcoCyc
  • toxin-antitoxin pair type II binding Source: EcoCyc

GO - Biological processi

  • negative regulation of transcription, DNA-templated Source: EcoCyc
  • regulation of cell growth Source: EcoCyc
  • transcription, DNA-templated Source: UniProtKB-KW

Keywordsi

Molecular functionDNA-binding, Repressor
Biological processToxin-antitoxin system, Transcription, Transcription regulation

Enzyme and pathway databases

BioCyciEcoCyc:EG10955-MONOMER
MetaCyc:EG10955-MONOMER

Names & Taxonomyi

Protein namesi
Recommended name:
Antitoxin PrlF
Alternative name(s):
HtrA suppressor protein SohA
Gene namesi
Name:prlF
Synonyms:sohA
Ordered Locus Names:b3129, JW3098
OrganismiEscherichia coli (strain K12)
Taxonomic identifieri83333 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaEnterobacteralesEnterobacteriaceaeEscherichia
Proteomesi
  • UP000000318 Componenti: Chromosome
  • UP000000625 Componenti: Chromosome

Organism-specific databases

EcoGeneiEG10955 prlF

Subcellular locationi

GO - Cellular componenti

Keywords - Cellular componenti

Cytoplasm

Pathology & Biotechi

Disruption phenotypei

Not essential.1 Publication

Mutagenesis

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Mutagenesisi91 – 111QGKKL…IGDDE → HSTRKETCRWHGRQH in prlF1; suppresses overproduction lethality of lamB-lacZ gene fusions, leads to decreased beta-galactosidase activity. Partially alleviates YhaV toxic activity. 1 PublicationAdd BLAST21

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00000720331 – 111Antitoxin PrlFAdd BLAST111

Proteomic databases

PaxDbiP15373
PRIDEiP15373

Interactioni

Subunit structurei

Homodimer; forms a complex with YhaV with stoichiometry PrlF2-YhaV4, possibly as a YhaV2-PrlF2-YhaV2 complex like the MazFE complex. This complex is seen to dimerize in solution.1 Publication

GO - Molecular functioni

  • enzyme binding Source: EcoCyc
  • identical protein binding Source: EcoCyc
  • toxin-antitoxin pair type II binding Source: EcoCyc

Protein-protein interaction databases

BioGridi4259492, 399 interactors
IntActiP15373, 6 interactors
STRINGi316385.ECDH10B_3302

Structurei

3D structure databases

ProteinModelPortaliP15373
SMRiP15373
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini12 – 59SpoVT-AbrBPROSITE-ProRule annotationAdd BLAST48

Phylogenomic databases

eggNOGiENOG4105ZEG Bacteria
COG2002 LUCA
HOGENOMiHOG000229555
InParanoidiP15373
KOiK19156
OMAiDKICYTI
PhylomeDBiP15373

Family and domain databases

InterProiView protein in InterPro
IPR031848 PrlF_antitoxin
IPR007159 SpoVT-AbrB_dom
IPR037914 SpoVT-AbrB_sf
PfamiView protein in Pfam
PF15937 PrlF_antitoxin, 1 hit
SUPFAMiSSF89447 SSF89447, 1 hit
PROSITEiView protein in PROSITE
PS51740 SPOVT_ABRB, 1 hit

Sequencei

Sequence statusi: Complete.

P15373-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MPANARSHAV LTTESKVTIR GQTTIPAPVR EALKLKPGQD SIHYEILPGG
60 70 80 90 100
QVFMCRLGDE QEDHTMNAFL RFLDADIQNN PQKTRPFNIQ QGKKLVAGMD
110
VNIDDEIGDD E
Length:111
Mass (Da):12,359
Last modified:April 1, 1990 - v1
Checksum:i5FC0D5FF43F75D8A
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
M30178 Genomic DNA Translation: AAA24638.1
M32358 Genomic DNA Translation: AAA24418.1
U18997 Genomic DNA Translation: AAA57932.1
U00096 Genomic DNA Translation: AAC76163.1
AP009048 Genomic DNA Translation: BAE77176.1
PIRiA35137
RefSeqiNP_417598.1, NC_000913.3
WP_001307405.1, NZ_LN832404.1

Genome annotation databases

EnsemblBacteriaiAAC76163; AAC76163; b3129
BAE77176; BAE77176; BAE77176
GeneIDi947639
KEGGiecj:JW3098
eco:b3129
PATRICifig|1411691.4.peg.3602

Similar proteinsi

Entry informationi

Entry nameiPRLF_ECOLI
AccessioniPrimary (citable) accession number: P15373
Secondary accession number(s): Q2M980
Entry historyiIntegrated into UniProtKB/Swiss-Prot: April 1, 1990
Last sequence update: April 1, 1990
Last modified: March 28, 2018
This is version 127 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome
UniProt is an ELIXIR core data resource
Main funding by: National Institutes of Health