Reviewed, UniProtKB/Swiss-Prot P15370 (EGON_DROME)

Names and origin

Protein names

Recommended name:
    Protein embryonic gonad
Alternative name(s):
    Protein eagle
    Protein spready
    Nuclear receptor subfamily 0 group A member 3

Gene names

Name:	eg
Synonyms:	egon, NR0A3, spry
ORF Names:	CG7383

Organism

Drosophila melanogaster (Fruit fly) [Complete proteome]

Taxonomic identifier

7227 [NCBI]

Taxonomic lineage

Eukaryota › Metazoa › Arthropoda › Hexapoda › Insecta › Pterygota › Neoptera › Endopterygota › Diptera › Brachycera › Muscomorpha › Ephydroidea › Drosophilidae › Drosophila › Sophophora

Protein attributes

Sequence length	373 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Evidence at protein level.

General annotation (Comments)

Function	May play a critical role in development of the progeny of eagle-positive neuroblasts. Ref.2
Subcellular location	Nucleus Potential.
Tissue specificity	During embryonic stages 10 and 11, expression was observed in four neuroblasts, NB2-4, NB3-3, NB6-4 and NB7-3. Except for NB6-4, expression reaches a maximum at the very beginning of expression or in the period of neuroblast delamination. Weak expression is also observed in a few putative progeny of NB7-3 during late embryonic stage 11 and 12. All eagle expression in abdominal segments disappears by stage 13. Ref.2
Disruption phenotype	EG cells fail to acquire the neural fate or underwent an extremely delayed differentiation, while EW neurons produced neurites in abnormal directions. Ref.2
Sequence similarities	Belongs to the nuclear hormone receptor family. NR0 subfamily. Contains 1 nuclear receptor DNA-binding domain.

Ontologies

Keywords
Biological process	Differentiation Neurogenesis Transcription Transcription regulation
Cellular component	Nucleus
Domain	Zinc-finger
Ligand	DNA-binding Metal-binding Zinc
Molecular function	Developmental protein Receptor
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	cell differentiation Inferred from electronic annotation. Source: UniProtKB-KW central nervous system development Inferred from mutant phenotype. Source: FlyBase regulation of transcription, DNA-dependent Inferred from electronic annotation. Source: UniProtKB-KW transcription Inferred from electronic annotation. Source: UniProtKB-KW
Cellular component	nucleus Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	ligand-dependent nuclear receptor activity Non-traceable author statement. Source: FlyBase protein binding Inferred from physical interaction. Source: IntAct sequence-specific DNA binding Inferred from electronic annotation. Source: InterPro transcription factor activity Inferred from electronic annotation. Source: InterPro zinc ion binding Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Binary interactions

With	Entry	#Exp.	IntAct	Notes
CtBP	O46036	1	EBI-171662,EBI-159330
HLHm5	P13096	1	EBI-171662,EBI-104760
HLHmbeta	Q01069	1	EBI-171662,EBI-94554

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 373	373	Protein embryonic gonad		PRO_0000053746
Regions
	DNA binding	2 – 78	77	Nuclear receptor
	Zinc finger	5 – 25	21	NR C4-type
	Zinc finger	42 – 66	25	NR C4-type
Experimental info
	Sequence conflict	325	1	P → H in CAA34626. Ref.1
	Sequence conflict	325	1	P → H in BAA07742. Ref.2
	Sequence conflict	325	1	P → H in BAA07743. Ref.2

Sequences

	Sequence		Length	Mass (Da)
	P15370-1 [UniParc]. Last modified June 26, 2007. Version 2. Checksum: 137494333A9A5760	FASTA	373	39,561
	10 20 30 40 50 60 MNQLCKVCGE PAAGFHFGAF TCEGCKSFFG RTYNNIAAIA GCKHNGDCVI NKKNRTACKA 70 80 90 100 110 120 CRLRKCLLVG MSKSGSRYGR RSNWFKIHCL LQEQQTTSGL GGGSSVGSGS GGGVSSASLE 130 140 150 160 170 180 QLARLQQASN QARQTYQDKT NPCIKSATAT TSPRIEGAAV GTGIGGGASP SFLQAAKLHH 190 200 210 220 230 240 QRQLKLDSRL SNTPSDSGAS SAGDPNEDGV TSVLGGQIAT PSSTNATSLP KLDLRHPNFP 250 260 270 280 290 300 ATSEPDADMQ RQRHQELLEI FRSHSEPLYS SFAPFSHLPP VLLAAGVPQL PIFKDQFKAE 310 320 330 340 350 360 LLFPTTSSPE LEEPIDLSFR SRADPASPMA HNSNSPSLSE PAAASHCLGE STNFVRKSTP 370 LDLTLVRSQT LTG

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Three hormone receptor-like Drosophila genes encode an identical DNA-binding finger." Rothe M., Nauber U., Jaeckle H. EMBO J. 8:3087-3094(1989) [PubMed: 2555153] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA].
[2]	"Eagle, a member of the steroid receptor gene superfamily, is expressed in a subset of neuroblasts and regulates the fate of their putative progeny in the Drosophila CNS." Higashijima S., Shishido E., Matsuzaki M., Saigo K. Development 122:527-536(1996) [PubMed: 8625804] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA], FUNCTION, TISSUE SPECIFICITY, DISRUPTION PHENOTYPE. Strain: Canton-S.
[3]	"The genome sequence of Drosophila melanogaster." Adams M.D., Celniker S.E., Holt R.A., Evans C.A., Gocayne J.D., Amanatides P.G., Scherer S.E., Li P.W., Hoskins R.A., Galle R.F., George R.A., Lewis S.E., Richards S., Ashburner M., Henderson S.N., Sutton G.G., Wortman J.R., Yandell M.D. , Zhang Q., Chen L.X., Brandon R.C., Rogers Y.-H.C., Blazej R.G., Champe M., Pfeiffer B.D., Wan K.H., Doyle C., Baxter E.G., Helt G., Nelson C.R., Miklos G.L.G., Abril J.F., Agbayani A., An H.-J., Andrews-Pfannkoch C., Baldwin D., Ballew R.M., Basu A., Baxendale J., Bayraktaroglu L., Beasley E.M., Beeson K.Y., Benos P.V., Berman B.P., Bhandari D., Bolshakov S., Borkova D., Botchan M.R., Bouck J., Brokstein P., Brottier P., Burtis K.C., Busam D.A., Butler H., Cadieu E., Center A., Chandra I., Cherry J.M., Cawley S., Dahlke C., Davenport L.B., Davies P., de Pablos B., Delcher A., Deng Z., Mays A.D., Dew I., Dietz S.M., Dodson K., Doup L.E., Downes M., Dugan-Rocha S., Dunkov B.C., Dunn P., Durbin K.J., Evangelista C.C., Ferraz C., Ferriera S., Fleischmann W., Fosler C., Gabrielian A.E., Garg N.S., Gelbart W.M., Glasser K., Glodek A., Gong F., Gorrell J.H., Gu Z., Guan P., Harris M., Harris N.L., Harvey D.A., Heiman T.J., Hernandez J.R., Houck J., Hostin D., Houston K.A., Howland T.J., Wei M.-H., Ibegwam C., Jalali M., Kalush F., Karpen G.H., Ke Z., Kennison J.A., Ketchum K.A., Kimmel B.E., Kodira C.D., Kraft C.L., Kravitz S., Kulp D., Lai Z., Lasko P., Lei Y., Levitsky A.A., Li J.H., Li Z., Liang Y., Lin X., Liu X., Mattei B., McIntosh T.C., McLeod M.P., McPherson D., Merkulov G., Milshina N.V., Mobarry C., Morris J., Moshrefi A., Mount S.M., Moy M., Murphy B., Murphy L., Muzny D.M., Nelson D.L., Nelson D.R., Nelson K.A., Nixon K., Nusskern D.R., Pacleb J.M., Palazzolo M., Pittman G.S., Pan S., Pollard J., Puri V., Reese M.G., Reinert K., Remington K., Saunders R.D.C., Scheeler F., Shen H., Shue B.C., Siden-Kiamos I., Simpson M., Skupski M.P., Smith T.J., Spier E., Spradling A.C., Stapleton M., Strong R., Sun E., Svirskas R., Tector C., Turner R., Venter E., Wang A.H., Wang X., Wang Z.-Y., Wassarman D.A., Weinstock G.M., Weissenbach J., Williams S.M., Woodage T., Worley K.C., Wu D., Yang S., Yao Q.A., Ye J., Yeh R.-F., Zaveri J.S., Zhan M., Zhang G., Zhao Q., Zheng L., Zheng X.H., Zhong F.N., Zhong W., Zhou X., Zhu S.C., Zhu X., Smith H.O., Gibbs R.A., Myers E.W., Rubin G.M., Venter J.C. Science 287:2185-2195(2000) [PubMed: 10731132] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: Berkeley.
[4]	"Annotation of the Drosophila melanogaster euchromatic genome: a systematic review." Misra S., Crosby M.A., Mungall C.J., Matthews B.B., Campbell K.S., Hradecky P., Huang Y., Kaminker J.S., Millburn G.H., Prochnik S.E., Smith C.D., Tupy J.L., Whitfield E.J., Bayraktaroglu L., Berman B.P., Bettencourt B.R., Celniker S.E., de Grey A.D.N.J. , Drysdale R.A., Harris N.L., Richter J., Russo S., Schroeder A.J., Shu S.Q., Stapleton M., Yamada C., Ashburner M., Gelbart W.M., Rubin G.M., Lewis S.E. Genome Biol. 3:RESEARCH0083.1-RESEARCH0083.22(2002) [PubMed: 12537572] [Abstract] Cited for: GENOME REANNOTATION.
[5]	Stapleton M., Carlson J.W., Frise E., Kapadia B., Park S., Wan K.H., Yu C., Celniker S.E. Submitted (OCT-2006) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. Strain: Berkeley. Tissue: Larva and Pupae.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBLGenBankDDBJ	X16631 mRNA. Translation: CAA34626.1. D43634 mRNA. Translation: BAA07742.1. D43635 mRNA. Translation: BAA07743.1. AE014296 Genomic DNA. Translation: AAF51783.1. AE014296 Genomic DNA. Translation: AAN12188.1. BT028846 mRNA. Translation: ABI34227.2.
PIR	S06010.
RefSeq	NP_524206.1. NP_730689.1.
UniGene	Dm.2504
3D structure databases
HSSP	HSSP built from PDB template 1KB6 based on UniProtKB P11473.
ModBase	Search...
Protein-protein interaction databases
IntAct	P15370. 3 interactions.
Genome annotation databases
Ensembl	FBgn0000560. Drosophila melanogaster. [Contig view]
GeneID	40428.
KEGG	dme:Dmel_CG7383.
Organism-specific databases
FlyBase	FBgn0000560. eg.
Phylogenomic databases
HOGENOM	P15370.
OMA	P15370. FRSHSEP.
Gene expression databases
ArrayExpress	P15370.
GermOnline	CG7383. Drosophila melanogaster.
Family and domain databases
InterPro	IPR000324. VitD_rcpt. IPR001628. Znf_hrmn_rcpt. IPR013088. Znf_NHR/GATA. [Graphical view]
Gene3D	G3DSA:3.30.50.10. Znf_NHR/GATA. 1 hit.
Pfam	PF00105. zf-C4. 1 hit. [Graphical view]
PRINTS	PR00047. STROIDFINGER. PR00350. VITAMINDR.
ProDom	PD000035. Znf_C4steroid. 1 hit. [Graphical view] [Entries sharing at least one domain]
SMART	SM00399. ZnF_C4. 1 hit. [Graphical view]
PROSITE	PS00031. NUCLEAR_REC_DBD_1. 1 hit. PS51030. NUCLEAR_REC_DBD_2. 1 hit. [Graphical view]
ProtoNet	Search...
Other Resources
NextBio	818724.

Entry information

Entry name

EGON_DROME

Accession

Primary (citable) accession number: P15370
Secondary accession number(s): Q0IGS4, Q9VNY0

Entry history

Integrated into UniProtKB/Swiss-Prot:	April 1, 1990
Last sequence update:	June 26, 2007
Last modified:	June 16, 2009
This is version 88 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

Drosophila annotation project