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Protein

Scytalidopepsin B

Gene
N/A
Organism
Scytalidium lignicola (Hyphomycete)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Experimental evidence at protein leveli

Functioni

Catalytic activityi

Hydrolysis of proteins with broad specificity, cleaving 24-Phe-|-Phe-25, but not 15-Leu-|-Tyr-16 and 25-Phe-|-Tyr-26 in the B chain of insulin.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sitei107Transition state stabilizer1
Active sitei190Proton acceptor1 Publication1

GO - Molecular functioni

Complete GO annotation...

Keywords - Molecular functioni

Aspartyl protease, Hydrolase, Protease

Protein family/group databases

MEROPSiG01.001.

Names & Taxonomyi

Protein namesi
Recommended name:
Scytalidopepsin B (EC:3.4.23.32)
Short name:
SLB
Alternative name(s):
Acid protease B
OrganismiScytalidium lignicola (Hyphomycete)
Taxonomic identifieri5539 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaPezizomycotinaLeotiomycetesmitosporic LeotiomycetesScytalidium

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Signal peptidei1 – 20Sequence analysisAdd BLAST20
PropeptideiPRO_000002849321 – 541 PublicationAdd BLAST34
ChainiPRO_000002849455 – 260Scytalidopepsin BAdd BLAST206

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Disulfide bondi101 ↔ 1811 Publication
Disulfide bondi195 ↔ 2191 Publication
Disulfide bondi248 ↔ 2571 Publication

Keywords - PTMi

Disulfide bond, Zymogen

Interactioni

Subunit structurei

Monomer.

Structurei

Secondary structure

1260
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details
Feature keyPosition(s)DescriptionActionsGraphical viewLength
Beta strandi56 – 58Combined sources3
Beta strandi60 – 66Combined sources7
Beta strandi70 – 78Combined sources9
Beta strandi81 – 96Combined sources16
Beta strandi98 – 100Combined sources3
Beta strandi105 – 114Combined sources10
Beta strandi118 – 127Combined sources10
Beta strandi129 – 131Combined sources3
Beta strandi142 – 151Combined sources10
Beta strandi154 – 161Combined sources8
Turni162 – 165Combined sources4
Beta strandi166 – 172Combined sources7
Beta strandi184 – 190Combined sources7
Beta strandi193 – 195Combined sources3
Turni197 – 200Combined sources4
Beta strandi202 – 204Combined sources3
Beta strandi209 – 212Combined sources4
Beta strandi214 – 223Combined sources10
Beta strandi226 – 228Combined sources3
Helixi230 – 232Combined sources3
Beta strandi234 – 236Combined sources3
Beta strandi246 – 251Combined sources6
Beta strandi254 – 259Combined sources6

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
1S2BX-ray2.10A55-260[»]
1S2KX-ray2.00A55-260[»]
2IFRX-ray1.95A55-260[»]
2IFWX-ray2.30A/B55-260[»]
ProteinModelPortaliP15369.
SMRiP15369.
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiP15369.

Family & Domainsi

Sequence similaritiesi

Belongs to the peptidase G1 family.Curated

Keywords - Domaini

Signal

Phylogenomic databases

KOiK20743.

Family and domain databases

CDDicd13425. Peptidase_G1_like. 1 hit.
InterProiIPR013320. ConA-like_dom.
IPR000250. Peptidase_G1.
IPR033863. Scytalidoglutamic_peptidase.
[Graphical view]
PfamiPF01828. Peptidase_A4. 1 hit.
[Graphical view]
PRINTSiPR00977. SCYTLDPTASE.
SUPFAMiSSF49899. SSF49899. 1 hit.

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

P15369-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MKFTTAAVLS ALVSAEIAFA APGGNGFARR QARRQARAAG LKASPFRQVN
60 70 80 90 100
AKEATVESNW GGAILIGSDF DTVSATANVP SASGGSSAAG TAWVGIDGDT
110 120 130 140 150
CQTAILQTGF DWYGDGTYDA WYEWYPEVSD DFSGITISEG DSIQMSVTAT
160 170 180 190 200
SDTSGSATLE NLTTGQKVSK SFSNESSGSL CRTNAEFIIE DFEECNSNGS
210 220 230 240 250
DCEFVPFASF SPAVEFTDCS VTSDGESVSL DDAQITQVII NNQDVTDCSV
260
SGTTVSCSYV
Length:260
Mass (Da):27,165
Last modified:September 26, 2003 - v2
Checksum:iD73852833694C6E0
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti83 – 91SGGSSAAGT → TGASGGSSA AA sequence (PubMed:6370989).Curated9
Sequence conflicti179Missing AA sequence (PubMed:6370989).Curated1
Sequence conflicti198N → D AA sequence (PubMed:6370989).Curated1
Sequence conflicti202Missing AA sequence (PubMed:6370989).Curated1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AB038553 mRNA. Translation: BAA92164.1.
D83963 Genomic DNA. Translation: BAA12157.1.
PIRiA28864.
JC4883.
JE0300.

Genome annotation databases

KEGGiag:BAA92164.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AB038553 mRNA. Translation: BAA92164.1.
D83963 Genomic DNA. Translation: BAA12157.1.
PIRiA28864.
JC4883.
JE0300.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
1S2BX-ray2.10A55-260[»]
1S2KX-ray2.00A55-260[»]
2IFRX-ray1.95A55-260[»]
2IFWX-ray2.30A/B55-260[»]
ProteinModelPortaliP15369.
SMRiP15369.
ModBaseiSearch...
MobiDBiSearch...

Protein family/group databases

MEROPSiG01.001.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

KEGGiag:BAA92164.

Phylogenomic databases

KOiK20743.

Miscellaneous databases

EvolutionaryTraceiP15369.

Family and domain databases

CDDicd13425. Peptidase_G1_like. 1 hit.
InterProiIPR013320. ConA-like_dom.
IPR000250. Peptidase_G1.
IPR033863. Scytalidoglutamic_peptidase.
[Graphical view]
PfamiPF01828. Peptidase_A4. 1 hit.
[Graphical view]
PRINTSiPR00977. SCYTLDPTASE.
SUPFAMiSSF49899. SSF49899. 1 hit.
ProtoNetiSearch...

Entry informationi

Entry nameiPRTB_SCYLI
AccessioniPrimary (citable) accession number: P15369
Secondary accession number(s): Q92333, Q9P962
Entry historyi
Integrated into UniProtKB/Swiss-Prot: April 1, 1990
Last sequence update: September 26, 2003
Last modified: November 30, 2016
This is version 94 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Direct protein sequencing

Documents

  1. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  2. Peptidase families
    Classification of peptidase families and list of entries
  3. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.