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P15367

- SEC11_YEAST

UniProt

P15367 - SEC11_YEAST

Protein

Signal peptidase complex catalytic subunit SEC11

Gene

SEC11

Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at protein leveli
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    • History
      Entry version 136 (01 Oct 2014)
      Sequence version 2 (01 Feb 1995)
      Previous versions | rss
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    Functioni

    Catalytic component of the signal peptidase complex (SPC), which catalyzes the cleavage of N-terminal signal sequences of proteins targeted to the endoplasmic reticulum. Signal peptide cleavage occurs during the translocation (cotranslationally or post-translationally) through the translocon pore into the endoplasmic reticulum.4 Publications

    Catalytic activityi

    Cleavage of hydrophobic, N-terminal signal or leader sequences from secreted and periplasmic proteins.

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Active sitei44 – 441By similarity

    GO - Molecular functioni

    1. peptidase activity Source: SGD
    2. protein binding Source: IntAct
    3. serine-type peptidase activity Source: InterPro

    GO - Biological processi

    1. protein targeting to ER Source: SGD
    2. signal peptide processing Source: SGD

    Keywords - Molecular functioni

    Hydrolase, Protease

    Enzyme and pathway databases

    BioCyciYEAST:YIR022W-MONOMER.

    Protein family/group databases

    MEROPSiS26.010.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Signal peptidase complex catalytic subunit SEC11 (EC:3.4.21.89)
    Alternative name(s):
    Secretory protein 11
    Signal peptidase I
    Gene namesi
    Name:SEC11
    Ordered Locus Names:YIR022W
    OrganismiSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
    Taxonomic identifieri559292 [NCBI]
    Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces
    ProteomesiUP000002311: Chromosome IX

    Organism-specific databases

    CYGDiYIR022w.
    SGDiS000001461. SEC11.

    Subcellular locationi

    Endoplasmic reticulum membrane 2 Publications; Single-pass type II membrane protein 2 Publications

    GO - Cellular componenti

    1. endoplasmic reticulum Source: SGD
    2. integral component of membrane Source: UniProtKB-KW
    3. signal peptidase complex Source: SGD

    Keywords - Cellular componenti

    Endoplasmic reticulum, Membrane

    Pathology & Biotechi

    Disruption phenotypei

    Leads to accumulation of core-glycosylated glycoprotein precursors.1 Publication

    Mutagenesis

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Mutagenesisi44 – 441S → A or C: Nonviable. 1 Publication
    Mutagenesisi83 – 831H → A or K: Nonviable; no protein expressed. 1 Publication
    Mutagenesisi83 – 831H → F: Nonviable. 1 Publication
    Mutagenesisi103 – 1031D → E: Nonviable; no protein expressed. 1 Publication
    Mutagenesisi103 – 1031D → N: Nonviable. 1 Publication
    Mutagenesisi109 – 1091D → E: Nonviable. 1 Publication
    Mutagenesisi109 – 1091D → N: Nonviable; no protein expressed. 1 Publication

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 167167Signal peptidase complex catalytic subunit SEC11PRO_0000109540Add
    BLAST

    Amino acid modifications

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Glycosylationi121 – 1211N-linked (GlcNAc...)Sequence Analysis

    Keywords - PTMi

    Glycoprotein

    Proteomic databases

    MaxQBiP15367.
    PaxDbiP15367.

    Expressioni

    Gene expression databases

    GenevestigatoriP15367.

    Interactioni

    Subunit structurei

    Component of the signal peptidase complex (SPC), which consists of SPC1, SPC2, SPC3 and SEC11. SPC associates with the translocon complex.2 Publications

    Binary interactionsi

    WithEntry#Exp.IntActNotes
    SPC1P469654EBI-16513,EBI-17823
    SPC3Q121333EBI-16513,EBI-17829

    Protein-protein interaction databases

    BioGridi35013. 107 interactions.
    DIPiDIP-5667N.
    IntActiP15367. 4 interactions.
    MINTiMINT-486420.
    STRINGi4932.YIR022W.

    Structurei

    3D structure databases

    ProteinModelPortaliP15367.
    SMRiP15367. Positions 34-136.
    ModBaseiSearch...
    MobiDBiSearch...

    Topological domain

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Topological domaini1 – 99CytoplasmicSequence Analysis
    Topological domaini32 – 167136LumenalSequence AnalysisAdd
    BLAST

    Transmembrane

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Transmembranei10 – 3122Helical; Signal-anchor for type II membrane proteinSequence AnalysisAdd
    BLAST

    Family & Domainsi

    Sequence similaritiesi

    Belongs to the peptidase S26B family.Curated

    Keywords - Domaini

    Signal-anchor, Transmembrane, Transmembrane helix

    Phylogenomic databases

    eggNOGiCOG0681.
    GeneTreeiENSGT00390000015600.
    HOGENOMiHOG000111516.
    KOiK13280.
    OMAiTKGDNNM.
    OrthoDBiEOG7BCNQ4.

    Family and domain databases

    InterProiIPR019758. Pept_S26A_signal_pept_1_CS.
    IPR019756. Pept_S26A_signal_pept_1_Ser-AS.
    IPR019759. Peptidase_S24_S26.
    IPR015927. Peptidase_S24_S26A/B/C.
    IPR001733. Peptidase_S26B.
    [Graphical view]
    PANTHERiPTHR10806. PTHR10806. 1 hit.
    PfamiPF00717. Peptidase_S24. 1 hit.
    [Graphical view]
    PRINTSiPR00728. SIGNALPTASE.
    SUPFAMiSSF51306. SSF51306. 1 hit.
    TIGRFAMsiTIGR02228. sigpep_I_arch. 1 hit.
    PROSITEiPS00501. SPASE_I_1. 1 hit.
    PS00761. SPASE_I_3. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    P15367-1 [UniParc]FASTAAdd to Basket

    « Hide

    MNLRFELQKL LNVCFLFASA YMFWQGLAIA TNSASPIVVV LSGSMEPAFQ    50
    RGDILFLWNR NTFNQVGDVV VYEVEGKQIP IVHRVLRQHN NHADKQFLLT 100
    KGDNNAGNDI SLYANKKIYL NKSKEIVGTV KGYFPQLGYI TIWISENKYA 150
    KFALLGMLGL SALLGGE 167
    Length:167
    Mass (Da):18,762
    Last modified:February 1, 1995 - v2
    Checksum:iFC877232D6888DF3
    GO

    Experimental Info

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Sequence conflicti162 – 1621A → R in CAA30533. (PubMed:3283143)Curated

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    X07694 Genomic DNA. Translation: CAA30533.1.
    Z38061 Genomic DNA. Translation: CAA86182.1.
    AY558239 Genomic DNA. Translation: AAS56565.1.
    BK006942 Genomic DNA. Translation: DAA08569.1.
    PIRiS48484.
    RefSeqiNP_012288.1. NM_001179544.1.

    Genome annotation databases

    EnsemblFungiiYIR022W; YIR022W; YIR022W.
    GeneIDi854840.
    KEGGisce:YIR022W.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    X07694 Genomic DNA. Translation: CAA30533.1 .
    Z38061 Genomic DNA. Translation: CAA86182.1 .
    AY558239 Genomic DNA. Translation: AAS56565.1 .
    BK006942 Genomic DNA. Translation: DAA08569.1 .
    PIRi S48484.
    RefSeqi NP_012288.1. NM_001179544.1.

    3D structure databases

    ProteinModelPortali P15367.
    SMRi P15367. Positions 34-136.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    BioGridi 35013. 107 interactions.
    DIPi DIP-5667N.
    IntActi P15367. 4 interactions.
    MINTi MINT-486420.
    STRINGi 4932.YIR022W.

    Protein family/group databases

    MEROPSi S26.010.

    Proteomic databases

    MaxQBi P15367.
    PaxDbi P15367.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    EnsemblFungii YIR022W ; YIR022W ; YIR022W .
    GeneIDi 854840.
    KEGGi sce:YIR022W.

    Organism-specific databases

    CYGDi YIR022w.
    SGDi S000001461. SEC11.

    Phylogenomic databases

    eggNOGi COG0681.
    GeneTreei ENSGT00390000015600.
    HOGENOMi HOG000111516.
    KOi K13280.
    OMAi TKGDNNM.
    OrthoDBi EOG7BCNQ4.

    Enzyme and pathway databases

    BioCyci YEAST:YIR022W-MONOMER.

    Miscellaneous databases

    NextBioi 977719.

    Gene expression databases

    Genevestigatori P15367.

    Family and domain databases

    InterProi IPR019758. Pept_S26A_signal_pept_1_CS.
    IPR019756. Pept_S26A_signal_pept_1_Ser-AS.
    IPR019759. Peptidase_S24_S26.
    IPR015927. Peptidase_S24_S26A/B/C.
    IPR001733. Peptidase_S26B.
    [Graphical view ]
    PANTHERi PTHR10806. PTHR10806. 1 hit.
    Pfami PF00717. Peptidase_S24. 1 hit.
    [Graphical view ]
    PRINTSi PR00728. SIGNALPTASE.
    SUPFAMi SSF51306. SSF51306. 1 hit.
    TIGRFAMsi TIGR02228. sigpep_I_arch. 1 hit.
    PROSITEi PS00501. SPASE_I_1. 1 hit.
    PS00761. SPASE_I_3. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "SEC11 is required for signal peptide processing and yeast cell growth."
      Boehni P.C., Deshaies R.J., Schekman R.W.
      J. Cell Biol. 106:1035-1042(1988) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA], DISRUPTION PHENOTYPE.
      Strain: PBY408A.
    2. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: ATCC 204508 / S288c.
    3. Cited for: GENOME REANNOTATION.
      Strain: ATCC 204508 / S288c.
    4. Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
      Strain: ATCC 204508 / S288c.
    5. "Solubilization and characterization of yeast signal peptidase."
      YaDeau J.T., Blobel G.
      J. Biol. Chem. 264:2928-2934(1989) [PubMed] [Europe PMC] [Abstract]
      Cited for: FUNCTION, SUBCELLULAR LOCATION.
    6. "Yeast signal peptidase contains a glycoprotein and the Sec11 gene product."
      YaDeau J.T., Klein C., Blobel G.
      Proc. Natl. Acad. Sci. U.S.A. 88:517-521(1991) [PubMed] [Europe PMC] [Abstract]
      Cited for: IDENTIFICATION IN THE SIGNAL PEPTIDASE COMPLEX.
    7. "A mutation affecting signal peptidase inhibits degradation of an abnormal membrane protein in Saccharomyces cerevisiae."
      Mullins C., Lu Y., Campbell A., Fang H., Green N.
      J. Biol. Chem. 270:17139-17147(1995) [PubMed] [Europe PMC] [Abstract]
      Cited for: FUNCTION.
    8. "The yeast SPC22/23 homolog Spc3p is essential for signal peptidase activity."
      Meyer H.A., Hartmann E.
      J. Biol. Chem. 272:13159-13164(1997) [PubMed] [Europe PMC] [Abstract]
      Cited for: IDENTIFICATION IN THE SIGNAL PEPTIDASE COMPLEX.
    9. "The catalytic mechanism of endoplasmic reticulum signal peptidase appears to be distinct from most eubacterial signal peptidases."
      VanValkenburgh C., Chen X., Mullins C., Fang H., Green N.
      J. Biol. Chem. 274:11519-11525(1999) [PubMed] [Europe PMC] [Abstract]
      Cited for: FUNCTION, MUTAGENESIS OF SER-44; HIS-83; ASP-103 AND ASP-109.
    10. "Signal peptidase and oligosaccharyltransferase interact in a sequential and dependent manner within the endoplasmic reticulum."
      Chen X., VanValkenburgh C., Liang H., Fang H., Green N.
      J. Biol. Chem. 276:2411-2416(2001) [PubMed] [Europe PMC] [Abstract]
      Cited for: FUNCTION.
    11. Cited for: SUBCELLULAR LOCATION [LARGE SCALE ANALYSIS].
    12. Cited for: LEVEL OF PROTEIN EXPRESSION [LARGE SCALE ANALYSIS].

    Entry informationi

    Entry nameiSEC11_YEAST
    AccessioniPrimary (citable) accession number: P15367
    Secondary accession number(s): D6VVV3
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: April 1, 1990
    Last sequence update: February 1, 1995
    Last modified: October 1, 2014
    This is version 136 of the entry and version 2 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programFungal Protein Annotation Program

    Miscellaneousi

    Miscellaneous

    Present with 3150 molecules/cell in log phase SD medium.1 Publication

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. Peptidase families
      Classification of peptidase families and list of entries
    2. SIMILARITY comments
      Index of protein domains and families
    3. Yeast
      Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD
    4. Yeast chromosome IX
      Yeast (Saccharomyces cerevisiae) chromosome IX: entries and gene names

    External Data

    Dasty 3