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Protein

Signal peptidase complex catalytic subunit SEC11

Gene

SEC11

Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Catalytic component of the signal peptidase complex (SPC), which catalyzes the cleavage of N-terminal signal sequences of proteins targeted to the endoplasmic reticulum. Signal peptide cleavage occurs during the translocation (cotranslationally or post-translationally) through the translocon pore into the endoplasmic reticulum.4 Publications

Miscellaneous

Present with 3150 molecules/cell in log phase SD medium.1 Publication

Catalytic activityi

Cleavage of hydrophobic, N-terminal signal or leader sequences from secreted and periplasmic proteins.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Active sitei44By similarity1

GO - Molecular functioni

  • peptidase activity Source: SGD
  • serine-type peptidase activity Source: InterPro

GO - Biological processi

  • protein targeting to ER Source: SGD
  • signal peptide processing Source: SGD

Keywordsi

Molecular functionHydrolase, Protease

Enzyme and pathway databases

BioCyciYEAST:YIR022W-MONOMER.

Protein family/group databases

MEROPSiS26.010.

Names & Taxonomyi

Protein namesi
Recommended name:
Signal peptidase complex catalytic subunit SEC11 (EC:3.4.21.89)
Alternative name(s):
Secretory protein 11
Signal peptidase I
Gene namesi
Name:SEC11
Ordered Locus Names:YIR022W
OrganismiSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Taxonomic identifieri559292 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces
Proteomesi
  • UP000002311 Componenti: Chromosome IX

Organism-specific databases

EuPathDBiFungiDB:YIR022W.
SGDiS000001461. SEC11.

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cell wall Cytoskeleton Vacuole Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Topology

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Topological domaini1 – 9CytoplasmicSequence analysis9
Transmembranei10 – 31Helical; Signal-anchor for type II membrane proteinSequence analysisAdd BLAST22
Topological domaini32 – 167LumenalSequence analysisAdd BLAST136

Keywords - Cellular componenti

Endoplasmic reticulum, Membrane

Pathology & Biotechi

Disruption phenotypei

Leads to accumulation of core-glycosylated glycoprotein precursors.1 Publication

Mutagenesis

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Mutagenesisi44S → A or C: Nonviable. 1 Publication1
Mutagenesisi83H → A or K: Nonviable; no protein expressed. 1 Publication1
Mutagenesisi83H → F: Nonviable. 1 Publication1
Mutagenesisi103D → E: Nonviable; no protein expressed. 1 Publication1
Mutagenesisi103D → N: Nonviable. 1 Publication1
Mutagenesisi109D → E: Nonviable. 1 Publication1
Mutagenesisi109D → N: Nonviable; no protein expressed. 1 Publication1

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00001095401 – 167Signal peptidase complex catalytic subunit SEC11Add BLAST167

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Glycosylationi121N-linked (GlcNAc...) asparagineSequence analysis1

Keywords - PTMi

Glycoprotein

Proteomic databases

MaxQBiP15367.
PRIDEiP15367.
TopDownProteomicsiP15367.

Interactioni

Subunit structurei

Component of the signal peptidase complex (SPC), which consists of SPC1, SPC2, SPC3 and SEC11. SPC associates with the translocon complex.2 Publications

Binary interactionsi

Show more details

Protein-protein interaction databases

BioGridi35013. 224 interactors.
DIPiDIP-5667N.
IntActiP15367. 4 interactors.
MINTiMINT-486420.
STRINGi4932.YIR022W.

Structurei

3D structure databases

ProteinModelPortaliP15367.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the peptidase S26B family.Curated

Keywords - Domaini

Signal-anchor, Transmembrane, Transmembrane helix

Phylogenomic databases

GeneTreeiENSGT00390000015600.
HOGENOMiHOG000111516.
InParanoidiP15367.
KOiK13280.
OMAiYIPFVGY.
OrthoDBiEOG092C4ZUJ.

Family and domain databases

InterProiView protein in InterPro
IPR036286. LexA/Signal_pep-like_sf.
IPR019758. Pept_S26A_signal_pept_1_CS.
IPR019756. Pept_S26A_signal_pept_1_Ser-AS.
IPR015927. Peptidase_S24_S26A/B/C.
IPR001733. Peptidase_S26B.
PANTHERiPTHR10806. PTHR10806. 1 hit.
PfamiView protein in Pfam
PF00717. Peptidase_S24. 1 hit.
PRINTSiPR00728. SIGNALPTASE.
SUPFAMiSSF51306. SSF51306. 1 hit.
TIGRFAMsiTIGR02228. sigpep_I_arch. 1 hit.
PROSITEiView protein in PROSITE
PS00501. SPASE_I_1. 1 hit.
PS00761. SPASE_I_3. 1 hit.

Sequencei

Sequence statusi: Complete.

P15367-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MNLRFELQKL LNVCFLFASA YMFWQGLAIA TNSASPIVVV LSGSMEPAFQ
60 70 80 90 100
RGDILFLWNR NTFNQVGDVV VYEVEGKQIP IVHRVLRQHN NHADKQFLLT
110 120 130 140 150
KGDNNAGNDI SLYANKKIYL NKSKEIVGTV KGYFPQLGYI TIWISENKYA
160
KFALLGMLGL SALLGGE
Length:167
Mass (Da):18,762
Last modified:February 1, 1995 - v2
Checksum:iFC877232D6888DF3
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti162A → R in CAA30533 (PubMed:3283143).Curated1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X07694 Genomic DNA. Translation: CAA30533.1.
Z38061 Genomic DNA. Translation: CAA86182.1.
AY558239 Genomic DNA. Translation: AAS56565.1.
BK006942 Genomic DNA. Translation: DAA08569.1.
PIRiS48484.
RefSeqiNP_012288.1. NM_001179544.1.

Genome annotation databases

EnsemblFungiiYIR022W; YIR022W; YIR022W.
GeneIDi854840.
KEGGisce:YIR022W.

Similar proteinsi

Entry informationi

Entry nameiSEC11_YEAST
AccessioniPrimary (citable) accession number: P15367
Secondary accession number(s): D6VVV3
Entry historyiIntegrated into UniProtKB/Swiss-Prot: April 1, 1990
Last sequence update: February 1, 1995
Last modified: October 25, 2017
This is version 159 of the entry and version 2 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Peptidase families
    Classification of peptidase families and list of entries
  2. SIMILARITY comments
    Index of protein domains and families
  3. Yeast
    Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD
  4. Yeast chromosome IX
    Yeast (Saccharomyces cerevisiae) chromosome IX: entries and gene names