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Protein

Ubiquitin-40S ribosomal protein S27a

Gene

RpS27A

Organism
Drosophila melanogaster (Fruit fly)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Ubiquitin exists either covalently attached to another protein, or free (unanchored). When covalently bound, it is conjugated to target proteins via an isopeptide bond either as a monomer (monoubiquitin), a polymer linked via different Lys residues of the ubiquitin (polyubiquitin chains) or a linear polymer linked via the initiator Met of the ubiquitin (linear polyubiquitin chains). Polyubiquitin chains, when attached to a target protein, have different functions depending on the Lys residue of the ubiquitin that is linked: Lys-48-linked is involved in protein degradation via the proteasome. Linear polymer chains formed via attachment by the initiator Met lead to cell signaling. Ubiquitin is usually conjugated to Lys residues of target proteins, however, in rare cases, conjugation to Cys or Ser residues has been observed. When polyubiquitin is free (unanchored-polyubiquitin), it also has distinct roles, such as in activation of protein kinases, and in signaling (By similarity).By similarity
Ribosomal protein S27a is a component of the 40S subunit of the ribosome.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Binding sitei54Activating enzyme1
Sitei68Essential for function1
Binding sitei72Activating enzyme1

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Zinc fingeri121 – 144C4-typeAdd BLAST24

GO - Molecular functioni

  • metal ion binding Source: UniProtKB-KW
  • protein tag Source: FlyBase
  • structural constituent of ribosome Source: FlyBase

GO - Biological processi

  • cytoplasmic translation Source: FlyBase
  • Notch signaling pathway Source: Reactome
  • protein ubiquitination Source: FlyBase
Complete GO annotation...

Keywords - Molecular functioni

Ribonucleoprotein, Ribosomal protein

Keywords - Ligandi

Metal-binding, Zinc

Enzyme and pathway databases

ReactomeiR-DME-2122948. Activated NOTCH1 Transmits Signal to the Nucleus.
R-DME-2979096. NOTCH2 Activation and Transmission of Signal to the Nucleus.

Names & Taxonomyi

Protein namesi
Recommended name:
Ubiquitin-40S ribosomal protein S27a
Cleaved into the following 2 chains:
Gene namesi
Name:RpS27A
Synonyms:UB3-D, UBI-F80, Ubi-m
ORF Names:CG5271
OrganismiDrosophila melanogaster (Fruit fly)
Taxonomic identifieri7227 [NCBI]
Taxonomic lineageiEukaryotaMetazoaEcdysozoaArthropodaHexapodaInsectaPterygotaNeopteraEndopterygotaDipteraBrachyceraMuscomorphaEphydroideaDrosophilidaeDrosophilaSophophora
Proteomesi
  • UP000000803 Componenti: Chromosome 2L

Organism-specific databases

FlyBaseiFBgn0003942. RpS27A.

Subcellular locationi

Ubiquitin :

GO - Cellular componenti

  • cytosol Source: Reactome
  • cytosolic small ribosomal subunit Source: FlyBase
  • microtubule associated complex Source: FlyBase
  • nucleus Source: UniProtKB-SubCell
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm, Nucleus

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00003964821 – 76UbiquitinAdd BLAST76
ChainiPRO_000039648377 – 15640S ribosomal protein S27aAdd BLAST80

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Cross-linki48Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in ubiquitin)
Cross-linki76Glycyl lysine isopeptide (Gly-Lys) (interchain with K-? in acceptor proteins)

Keywords - PTMi

Isopeptide bond, Ubl conjugation

Proteomic databases

PaxDbiP15357.
PRIDEiP15357.

Expressioni

Gene expression databases

BgeeiFBgn0003942.
ExpressionAtlasiP15357. baseline.
GenevisibleiP15357. DM.

Interactioni

Subunit structurei

Ribosomal protein S27a is part of the 40S ribosomal subunit.By similarity

Protein-protein interaction databases

BioGridi60502. 89 interactors.
IntActiP15357. 7 interactors.
MINTiMINT-1554051.
STRINGi7227.FBpp0079606.

Structurei

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
4V6Welectron microscopy6.00Af77-156[»]
ProteinModelPortaliP15357.
SMRiP15357.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini1 – 76Ubiquitin-likePROSITE-ProRule annotationAdd BLAST76

Compositional bias

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Compositional biasi78 – 107Lys-richAdd BLAST30

Sequence similaritiesi

In the N-terminal section; belongs to the ubiquitin family.Curated
In the C-terminal section; belongs to the ribosomal protein S27Ae family.Curated
Contains 1 ubiquitin-like domain.PROSITE-ProRule annotation

Zinc finger

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Zinc fingeri121 – 144C4-typeAdd BLAST24

Keywords - Domaini

Zinc-finger

Phylogenomic databases

eggNOGiKOG0004. Eukaryota.
COG5272. LUCA.
InParanoidiP15357.
KOiK02977.
OMAiMSILKYY.
OrthoDBiEOG091G178I.
PhylomeDBiP15357.

Family and domain databases

InterProiIPR002906. Ribosomal_S27a.
IPR011332. Ribosomal_zn-bd.
IPR019956. Ubiquitin.
IPR029071. Ubiquitin-rel_dom.
IPR019954. Ubiquitin_CS.
IPR000626. Ubiquitin_dom.
[Graphical view]
PfamiPF01599. Ribosomal_S27. 1 hit.
PF00240. ubiquitin. 1 hit.
[Graphical view]
PRINTSiPR00348. UBIQUITIN.
SMARTiSM01402. Ribosomal_S27. 1 hit.
SM00213. UBQ. 1 hit.
[Graphical view]
SUPFAMiSSF54236. SSF54236. 1 hit.
SSF57829. SSF57829. 1 hit.
PROSITEiPS00299. UBIQUITIN_1. 1 hit.
PS50053. UBIQUITIN_2. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

P15357-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MQIFVKTLTG KTITLEVEPS DTIENVKAKI QDKEGIPPDQ QRLIFAGKQL
60 70 80 90 100
EDGRTLSDYN IQKESTLHLV LRLRGGAKKR KKKNYSTPKK IKHKRKKVKL
110 120 130 140 150
AVLKYYKVDE NGKIHRLRRE CPGENCGAGV FMAAHEDRHY CGKCNLTFVF

SKPEEK
Length:156
Mass (Da):17,940
Last modified:August 10, 2010 - v2
Checksum:i4FB9AC25B8E86EF5
GO

Sequence cautioni

The sequence CAA48871 differs from that shown. Reason: Erroneous gene model prediction.Curated

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
M22536 mRNA. Translation: AAA28998.1.
X69119 Genomic DNA. Translation: CAA48871.1. Sequence problems.
AE014134 Genomic DNA. Translation: AAF52941.1.
RefSeqiNP_476778.1. NM_057430.4.
UniGeneiDm.36737.
Dm.4835.

Genome annotation databases

EnsemblMetazoaiFBtr0080016; FBpp0079606; FBgn0003942.
GeneIDi34420.
KEGGidme:Dmel_CG5271.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
M22536 mRNA. Translation: AAA28998.1.
X69119 Genomic DNA. Translation: CAA48871.1. Sequence problems.
AE014134 Genomic DNA. Translation: AAF52941.1.
RefSeqiNP_476778.1. NM_057430.4.
UniGeneiDm.36737.
Dm.4835.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
4V6Welectron microscopy6.00Af77-156[»]
ProteinModelPortaliP15357.
SMRiP15357.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi60502. 89 interactors.
IntActiP15357. 7 interactors.
MINTiMINT-1554051.
STRINGi7227.FBpp0079606.

Proteomic databases

PaxDbiP15357.
PRIDEiP15357.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblMetazoaiFBtr0080016; FBpp0079606; FBgn0003942.
GeneIDi34420.
KEGGidme:Dmel_CG5271.

Organism-specific databases

CTDi6233.
FlyBaseiFBgn0003942. RpS27A.

Phylogenomic databases

eggNOGiKOG0004. Eukaryota.
COG5272. LUCA.
InParanoidiP15357.
KOiK02977.
OMAiMSILKYY.
OrthoDBiEOG091G178I.
PhylomeDBiP15357.

Enzyme and pathway databases

ReactomeiR-DME-2122948. Activated NOTCH1 Transmits Signal to the Nucleus.
R-DME-2979096. NOTCH2 Activation and Transmission of Signal to the Nucleus.

Miscellaneous databases

GenomeRNAii34420.
PROiP15357.

Gene expression databases

BgeeiFBgn0003942.
ExpressionAtlasiP15357. baseline.
GenevisibleiP15357. DM.

Family and domain databases

InterProiIPR002906. Ribosomal_S27a.
IPR011332. Ribosomal_zn-bd.
IPR019956. Ubiquitin.
IPR029071. Ubiquitin-rel_dom.
IPR019954. Ubiquitin_CS.
IPR000626. Ubiquitin_dom.
[Graphical view]
PfamiPF01599. Ribosomal_S27. 1 hit.
PF00240. ubiquitin. 1 hit.
[Graphical view]
PRINTSiPR00348. UBIQUITIN.
SMARTiSM01402. Ribosomal_S27. 1 hit.
SM00213. UBQ. 1 hit.
[Graphical view]
SUPFAMiSSF54236. SSF54236. 1 hit.
SSF57829. SSF57829. 1 hit.
PROSITEiPS00299. UBIQUITIN_1. 1 hit.
PS50053. UBIQUITIN_2. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiRS27A_DROME
AccessioniPrimary (citable) accession number: P15357
Secondary accession number(s): P68198
, Q0E8I1, Q9VKW6, Q9VQX7, Q9VZL4
Entry historyi
Integrated into UniProtKB/Swiss-Prot: April 1, 1990
Last sequence update: August 10, 2010
Last modified: November 30, 2016
This is version 135 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programDrosophila annotation project

Miscellaneousi

Miscellaneous

In Drosophila ubiquitin is encoded by 3 different genes. RpL40 and RpS27A genes code for a single copy of ubiquitin fused to the ribosomal proteins L40 and S27a, respectively. Ubi-p63E gene codes for a polyubiquitin precursor with exact head to tail repeats.
For a better understanding, features related to ubiquitin are only indicated for the first chain.

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

Documents

  1. Drosophila
    Drosophila: entries, gene names and cross-references to FlyBase
  2. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  3. Ribosomal proteins
    Ribosomal proteins families and list of entries
  4. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.