Reviewed,
UniProtKB/Swiss-Prot P15337 (CREB1_RAT)
Last modified
November 3, 2009.
Version 108.
History...
Clusters with 100%,
90%,
50% identity |
Documents (2) |
Third-party data |
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Names and origin
| Protein names | Recommended name: Cyclic AMP-responsive element-binding protein 1 Short name=cAMP-responsive element-binding protein 1 Short name=CREB-1 | ||||
| Gene names |
| ||||
| Organism | Rattus norvegicus (Rat) | ||||
| Taxonomic identifier | 10116 [NCBI] | ||||
| Taxonomic lineage | Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Glires › Rodentia › Sciurognathi › Muroidea › Muridae › Murinae › Rattus |
Protein attributes
| Sequence length | 341 AA. |
| Sequence status | Complete. |
| Sequence processing | The displayed sequence is not processed. |
| Protein existence | Evidence at protein level. |
General annotation (Comments)
| Function | This protein binds the cAMP response element (CRE), a sequence present in many viral and cellular promoters. CREB stimulates transcription on binding to the CRE. Transciption activation is enhanced by the TORC coactivators which act independently of Ser-133 phosphorylation. Implicated in synchronization of circadian rhythmicity By similarity. |
| Subunit structure | Interacts with PPRC1. Binds DNA as a dimer. Interacts, through the bZIP domain, with the coactivators TORC1/CRTC1, TORC2/CRTC2 and TORC3/CRTC3 By similarity. When phosphorylated on Ser-133, binds CREBBP. |
| Subcellular location | |
| Post-translational modification | Phosphorylation of Ser-133 allows CREBBP binding. Phosphorylated upon DNA damage, probably by ATM or ATR By similarity. Stimulated by phosphorylation. Phosphorylation of both Ser-142 and Ser-133 in the SCN regulates the activity of CREB and participate in circadian rhythm generation By similarity. CAMK4 is much more potent than CAMK2 in activating CREB. Phosphorylated by CAMK1 and CAMK4. |
| Sequence similarities | Belongs to the bZIP family. Contains 1 bZIP domain. Contains 1 KID (kinase-inducible) domain. |
Ontologies
Alternative products
| This entry describes 2 isoforms produced by alternative splicing. [Align] [Select] | ||||||
| Isoform 1 (identifier: P15337-1) Also known as: Alpha; This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry. | ||||||
| Isoform 2 (identifier: P15337-2) Also known as: Delta; The sequence of this isoform differs from the canonical sequence as follows: 88-101: Missing. |
Sequence annotation (Features)
| Feature key | Position(s) | Length | Description | Graphical view | Feature identifier | ||||||||
Molecule processing | |||||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
| Chain | 1 – 341 | 341 | Cyclic AMP-responsive element-binding protein 1 | PRO_0000076599 | |||||||||
Regions | |||||||||||||
| Domain | 101 – 160 | 60 | KID | ||||||||||
| Domain | 311 – 332 | 22 | Leucine-zipper | ||||||||||
| DNA binding | 284 – 309 | 26 | Basic motif | ||||||||||
Amino acid modifications | |||||||||||||
| Modified residue | 111 | 1 | Phosphoserine By similarity | ||||||||||
| Modified residue | 119 | 1 | Phosphothreonine By similarity | ||||||||||
| Modified residue | 133 | 1 | Phosphoserine; by CaMK1, CaMK2 and CaMK4 Ref.3 Ref.4 Ref.5 | ||||||||||
| Modified residue | 142 | 1 | Phosphoserine; by CaMK2 Ref.4 | ||||||||||
Natural variations | |||||||||||||
| Alternative sequence | 88 – 101 | 14 | Missing in isoform 2. | VSP_000598 | |||||||||
Experimental info | |||||||||||||
| Sequence conflict | 135 | 1 | R → K in CAA42619. Ref.2 | ||||||||||
| Sequence conflict | 319 | 1 | E → K in CAA42619. Ref.2 | ||||||||||
Secondary structure | |||||||||||||
Helix Strand Turn | |||||||||||||
| Helix | 120 – 128 | 9 | |||||||||||
| Helix | 133 – 144 | 12 | |||||||||||
Sequences
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References
| [1] | "A cluster of phosphorylation sites on the cyclic AMP-regulated nuclear factor CREB predicted by its sequence." Gonzalez G.A., Yamamoto K.K., Fischer W.H., Karr D., Menzel P., Biggs W. III, Vale W.W., Montminy M.R. Nature 337:749-752(1989) [PubMed: 2521922] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 1), PARTIAL PROTEIN SEQUENCE. |
| [2] | "Nucleotide and derived amino-acid sequences of the CRE-binding proteins from rat C6 glioma and HeLa cells." Short M.L., Manohar C.F., Furtado M.R., Ghadge G.D., Wolinsky S.M., Thimmapaya B., Jungmann R.A. Nucleic Acids Res. 19:4290-4290(1991) [PubMed: 1831258] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 2). |
| [3] | "CREB: a Ca(2+)-regulated transcription factor phosphorylated by calmodulin-dependent kinases." Sheng M., Thompson M.A., Greenberg M.E. Science 252:1427-1430(1991) [PubMed: 1646483] [Abstract] Cited for: PHOSPHORYLATION AT SER-133, PHOSPHORYLATION BY CAMK1 AND CAMK4. |
| [4] | "Differential activation of CREB by Ca2+/calmodulin-dependent protein kinases type II and type IV involves phosphorylation of a site that negatively regulates activity." Sun P., Enslen H., Myung P.S., Maurer R.A. Genes Dev. 8:2527-2539(1994) [PubMed: 7958915] [Abstract] Cited for: PHOSPHORYLATION AT SER-133 AND SER-142. |
| [5] | "Regulation of activating transcription factor-1 and the cAMP response element-binding protein by Ca2+/calmodulin-dependent protein kinases type I, II, and IV." Sun P., Lou L., Maurer R.A. J. Biol. Chem. 271:3066-3073(1996) [PubMed: 8621702] [Abstract] Cited for: PHOSPHORYLATION AT SER-133, PHOSPHORYLATION BY CAMK1. |
| [6] | "Structural analyses of CREB-CBP transcriptional activator-coactivator complexes by NMR spectroscopy: implications for mapping the boundaries of structural domains." Radhakrishnan I., Perez-Alvarado G.C., Parker D., Dyson H.J., Montminy M.R., Wright P.E. J. Mol. Biol. 287:859-865(1999) [PubMed: 10222196] [Abstract] Cited for: STRUCTURE BY NMR OF 119-146 IN COMPLEX WITH CREBBP. |
| + | Additional computationally mapped references. |
Cross-references
Sequence databases | |||||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
| X14788 mRNA. Translation: CAA32890.1. X60002 mRNA. Translation: CAA42619.1. | |||||||||||||
| IPI | IPI00208239. IPI00327194. | ||||||||||||
| PIR | A35663. S03343. S22299. | ||||||||||||
| RefSeq | NP_112279.1. NP_604392.1. | ||||||||||||
| UniGene | Rn.90061 | ||||||||||||
3D structure databases | |||||||||||||
| |||||||||||||
| SMR | P15337. Positions 285-339. | ||||||||||||
| DisProt | DP00080. | ||||||||||||
| ModBase | Search... | ||||||||||||
Protein-protein interaction databases | |||||||||||||
| IntAct | P15337. 1 interaction. | ||||||||||||
| STRING | P15337. | ||||||||||||
PTM databases | |||||||||||||
| PhosphoSite | P15337. | ||||||||||||
Genome annotation databases | |||||||||||||
| Ensembl | ENSRNOT00000018326; ENSRNOP00000018326; ENSRNOG00000013412; Rattus norvegicus. [Genome view] ENSRNOT00000049654; ENSRNOP00000049369; ENSRNOG00000013412; Rattus norvegicus. [Genome view] | ||||||||||||
| GeneID | 81646. | ||||||||||||
| KEGG | rno:81646. | ||||||||||||
| UCSC | NM_031017. rat. | ||||||||||||
Organism-specific databases | |||||||||||||
| CTD | 81646. | ||||||||||||
| RGD | 620218. Creb1. | ||||||||||||
Phylogenomic databases | |||||||||||||
| HOVERGEN | P15337. | ||||||||||||
| OMA | XKILNDL. | ||||||||||||
Gene expression databases | |||||||||||||
| ArrayExpress | P15337. | ||||||||||||
| Genevestigator | P15337. | ||||||||||||
| GermOnline | ENSRNOG00000013412. Rattus norvegicus. | ||||||||||||
Family and domain databases | |||||||||||||
| InterPro | IPR011616. bZIP_1. IPR003102. Coactivator_CBP_pKID. IPR001630. Leuzip_CREB. IPR004827. TF_bZIP. [Graphical view] | ||||||||||||
| Pfam | PF00170. bZIP_1. 1 hit. PF02173. pKID. 1 hit. [Graphical view] | ||||||||||||
| PRINTS | PR00041. LEUZIPPRCREB. | ||||||||||||
| SMART | SM00338. BRLZ. 1 hit. [Graphical view] | ||||||||||||
| PROSITE | PS50217. BZIP. 1 hit. PS00036. BZIP_BASIC. 1 hit. PS50953. KID. 1 hit. [Graphical view] | ||||||||||||
| ProtoNet | Search... | ||||||||||||
Other Resources | |||||||||||||
| NextBio | 615164. | ||||||||||||
Entry information
| Entry name | CREB1_RAT | ||||||||
| Accession | Primary (citable) accession number: P15337 | ||||||||
| Entry history |
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| Entry status | Reviewed (UniProtKB/Swiss-Prot) | ||||||||
| Annotation project | HPI (Human Proteome Initiative) | ||||||||
Relevant documents
| PDB cross-references Index of Protein Data Bank (PDB) cross-references |
| SIMILARITY comments Index of protein domains and families |

Clusters with


