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Protein

Embryonic polarity protein dorsal

Gene

dl

Organism
Drosophila melanogaster (Fruit fly)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Embryonic developmental protein (PubMed:2598266, PubMed:10072776). The lateral or ventral identity of a cell depends upon the concentration of this protein in its nucleus during the blastoderm stage (PubMed:2598266). A morphogenetic protein that specifically binds to the kappa B-related consensus sequence 5'-GRGAAAANCC-3', located in the enhancer region of zygotic genes such as Zen, Twist, Snail and Decapentaplegic. Mediates an immune response in larvae (PubMed:10072776). Part of a signaling pathway involving NF-kappa-B and Toll-related receptors, that functions in the apoptosis of unfit cells during cell competition (PubMed:25477468). May be part of a NF-kappa-B and Tollo signaling cascade that regulates development of the peripheral nervous system (PubMed:18000549).4 Publications

GO - Molecular functioni

  • chromatin binding Source: GO_Central
  • high mobility group box 1 binding Source: FlyBase
  • repressing transcription factor binding Source: FlyBase
  • RNA polymerase II regulatory region sequence-specific DNA binding Source: FlyBase
  • sequence-specific DNA binding Source: FlyBase
  • transcriptional activator activity, RNA polymerase II core promoter proximal region sequence-specific binding Source: FlyBase

GO - Biological processi

  • anterior/posterior pattern specification Source: FlyBase
  • defense response Source: FlyBase
  • dorsal/ventral axis specification Source: FlyBase
  • dorsal/ventral pattern formation Source: FlyBase
  • ectodermal cell fate specification Source: FlyBase
  • ectoderm development Source: FlyBase
  • gastrulation Source: FlyBase
  • gastrulation involving germ band extension Source: FlyBase
  • germ cell migration Source: FlyBase
  • heart development Source: FlyBase
  • I-kappaB kinase/NF-kappaB signaling Source: GO_Central
  • immune response Source: FlyBase
  • melanization defense response Source: FlyBase
  • mesodermal cell fate specification Source: FlyBase
  • mesoderm development Source: FlyBase
  • negative regulation of gene expression Source: FlyBase
  • negative regulation of transcription, DNA-templated Source: FlyBase
  • negative regulation of transcription from RNA polymerase II promoter Source: FlyBase
  • NIK/NF-kappaB signaling Source: GO_Central
  • peripheral nervous system neuron development Source: FlyBase
  • plasmatocyte differentiation Source: FlyBase
  • positive regulation of biosynthetic process of antibacterial peptides active against Gram-negative bacteria Source: FlyBase
  • positive regulation of transcription, DNA-templated Source: FlyBase
  • positive regulation of transcription from RNA polymerase II promoter Source: FlyBase
  • regulation of alternative mRNA splicing, via spliceosome Source: FlyBase
  • regulation of glucose metabolic process Source: FlyBase
  • regulation of hemocyte proliferation Source: FlyBase
  • regulation of transcription, DNA-templated Source: FlyBase
  • response to cytokine Source: GO_Central
  • Toll signaling pathway Source: FlyBase
  • ventral cord development Source: FlyBase
Complete GO annotation...

Keywords - Molecular functioni

Activator, Developmental protein

Keywords - Biological processi

Transcription, Transcription regulation

Keywords - Ligandi

DNA-binding

Enzyme and pathway databases

ReactomeiR-DME-1169091. Activation of NF-kappaB in B cells.
R-DME-1810476. RIP-mediated NFkB activation via ZBP1.
R-DME-202424. Downstream TCR signaling.
R-DME-209560. NF-kB is activated and signals survival.
R-DME-2871837. FCERI mediated NF-kB activation.
R-DME-3134963. DEx/H-box helicases activate type I IFN and inflammatory cytokines production.
R-DME-445989. TAK1 activates NFkB by phosphorylation and activation of IKKs complex.
R-DME-5607761. Dectin-1 mediated noncanonical NF-kB signaling.
R-DME-5607764. CLEC7A (Dectin-1) signaling.
R-DME-5621575. CD209 (DC-SIGN) signaling.
R-DME-5676590. NIK-->noncanonical NF-kB signaling.
R-DME-933542. TRAF6 mediated NF-kB activation.
SignaLinkiP15330.

Names & Taxonomyi

Protein namesi
Recommended name:
Embryonic polarity protein dorsal
Gene namesi
Name:dl
ORF Names:CG6667
OrganismiDrosophila melanogaster (Fruit fly)
Taxonomic identifieri7227 [NCBI]
Taxonomic lineageiEukaryotaMetazoaEcdysozoaArthropodaHexapodaInsectaPterygotaNeopteraEndopterygotaDipteraBrachyceraMuscomorphaEphydroideaDrosophilidaeDrosophilaSophophora
Proteomesi
  • UP000000803 Componenti: Chromosome 2L

Organism-specific databases

FlyBaseiFBgn0260632. dl.

Subcellular locationi

  • Cytoplasm
  • Nucleus

  • Note: In ventral regions it is first cytoplasmic, then the protein is relocalized in the nucleus. Its nuclear localization is essential to its function as a morphogen. In dorsal regions it remains cytoplasmic. Tamo negatively regulates nuclear import of dl. Emb in the nuclear pore complex is responsible for export of dl from the nucleus.
Isoform A :

GO - Cellular componenti

  • cytoplasm Source: FlyBase
  • cytosol Source: GO_Central
  • I-kappaB/NF-kappaB complex Source: GO_Central
  • neuromuscular junction Source: FlyBase
  • nucleus Source: FlyBase
  • subsynaptic reticulum Source: FlyBase
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm, Nucleus

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00002051641 – 999Embryonic polarity protein dorsalAdd BLAST999

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei312Phosphoserine; by PKASequence analysis1

Keywords - PTMi

Phosphoprotein

Proteomic databases

PaxDbiP15330.
PRIDEiP15330.

PTM databases

iPTMnetiP15330.

Expressioni

Tissue specificityi

In unchallenged larvae, expression of both isoforms is seen in fat body and gut (isoform A is more abundant). After immune challenge levels of both isoforms are enhanced.1 Publication

Developmental stagei

Isoform A is expressed maternally and both isoforms are expressed zygotically from 6-9 hours embryos through to adulthood.1 Publication

Gene expression databases

BgeeiFBgn0260632.
ExpressionAtlasiP15330. baseline.
GenevisibleiP15330. DM.

Interactioni

Subunit structurei

Interacts with tamo via the nuclear localization signal. Interacts with emb, a component of the nuclear pore complex.2 Publications

Binary interactionsi

WithEntry#Exp.IntActNotes
cactQ030174EBI-198375,EBI-200600
tamoQ9W1A44EBI-198375,EBI-91385
tubP228123EBI-198375,EBI-93181

GO - Molecular functioni

  • high mobility group box 1 binding Source: FlyBase
  • repressing transcription factor binding Source: FlyBase

Protein-protein interaction databases

BioGridi61043. 52 interactors.
DIPiDIP-17423N.
IntActiP15330. 5 interactors.
STRINGi7227.FBpp0080560.

Structurei

3D structure databases

ProteinModelPortaliP15330.
SMRiP15330.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini47 – 342RHDPROSITE-ProRule annotationAdd BLAST296

Motif

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Motifi756 – 773Nuclear localization signalSequence analysisAdd BLAST18

Compositional bias

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Compositional biasi395 – 464Gln-richAdd BLAST70
Compositional biasi635 – 705Pro-richAdd BLAST71

Sequence similaritiesi

Contains 1 RHD (Rel-like) domain.PROSITE-ProRule annotation

Phylogenomic databases

eggNOGiENOG410IG8F. Eukaryota.
ENOG410XT64. LUCA.
GeneTreeiENSGT00500000044765.
InParanoidiP15330.
KOiK09254.
OMAiREPSIGH.
OrthoDBiEOG091G07RW.
PhylomeDBiP15330.

Family and domain databases

CDDicd01177. IPT_NFkappaB. 1 hit.
Gene3Di2.60.40.10. 1 hit.
2.60.40.340. 1 hit.
InterProiIPR013783. Ig-like_fold.
IPR014756. Ig_E-set.
IPR002909. IPT.
IPR033926. IPT_NFkappaB.
IPR000451. NFkB/Dor.
IPR008967. p53-like_TF_DNA-bd.
IPR030492. RHD_CS.
IPR032397. RHD_dimer.
IPR011539. RHD_DNA_bind_dom.
[Graphical view]
PANTHERiPTHR24169. PTHR24169. 2 hits.
PfamiPF16179. RHD_dimer. 1 hit.
PF00554. RHD_DNA_bind. 1 hit.
[Graphical view]
PRINTSiPR00057. NFKBTNSCPFCT.
SMARTiSM00429. IPT. 1 hit.
[Graphical view]
SUPFAMiSSF49417. SSF49417. 1 hit.
SSF81296. SSF81296. 1 hit.
PROSITEiPS01204. REL_1. 1 hit.
PS50254. REL_2. 1 hit.
[Graphical view]

Sequences (2)i

Sequence statusi: Complete.

This entry describes 2 isoformsi produced by alternative splicing. AlignAdd to basket

Isoform C (identifier: P15330-1) [UniParc]FASTAAdd to basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MFPNQNNGAA PGQGPAVDGQ QSLNYNGLPA QQQQQLAQST KNVRKKPYVK
60 70 80 90 100
ITEQPAGKAL RFRYECEGRS AGSIPGVNST PENKTYPTIE IVGYKGRAVV
110 120 130 140 150
VVSCVTKDTP YRPHPHNLVG KEGCKKGVCT LEINSETMRA VFSNLGIQCV
160 170 180 190 200
KKKDIEAALK AREEIRVDPF KTGFSHRFQP SSIDLNSVRL CFQVFMESEQ
210 220 230 240 250
KGRFTSPLPP VVSEPIFDKK AMSDLVICRL CSCSATVFGN TQIILLCEKV
260 270 280 290 300
AKEDISVRFF EEKNGQSVWE AFGDFQHTDV HKQTAITFKT PRYHTLDITE
310 320 330 340 350
PAKVFIQLRR PSDGVTSEAL PFEYVPMDSG KHTFWNLHRH LKRKPDEDLF
360 370 380 390 400
QQILRLDAKR EVQPPTIEVI DLDTPKIDVQ REIPSEMEFN HEESQQSEPA
410 420 430 440 450
LEQEQSVQQE QYTQEQSLQQ EQYTQEQSLQ QEQYLQQLEQ QQSFQLEEPM
460 470 480 490 500
QQDQELPAQQ SFDQAIDHLP DHTSDHIPED MEAADAHAEA EAHRLRSEQE
510 520 530 540 550
KEIDTIIDEK VRELEQLDLG QQLEPRPLTA NDKITEWMKS SEIEQQVHEP
560 570 580 590 600
SPTAEADVLD SALEISKADK TLDELLETVA ELDEIYTDFK VQRDTYKNTI
610 620 630 640 650
QNELAGLQGR APLQVEDSFD DAATYTSLQI AFKNPVLIPM DDIMPPTPPM
660 670 680 690 700
SQCAPEDAHQ HYDPVEVNSQ ARKPETPMRP VPPVPPAILT IQYPPEEDKL
710 720 730 740 750
PPLPPKRIRK QDSNAENRSI EANTVQTKPS TGESPLNKRL PPAPKNPNFN
760 770 780 790 800
TLPRQKKPGF FSKLFSRRKS KPDLAQGQEN SSILDSKANS REPSIGHFNM
810 820 830 840 850
QDPMRASLRS SKSAAPFISN PAPAKSSPVK AKKPGSKLTK PVGRSVSSVS
860 870 880 890 900
GKRPAYLNAD VVHIPLKGDS VNSLPQQQRT EGYSQSSTIS VGAGLDRRTA
910 920 930 940 950
SALQLADIPI SEGGMELVAI ADRQSLHNLV SSIEGHFNVQ LDPNLDLTEA
960 970 980 990
EHFALYTSIP PLAAASEFDE TSAYYAPVDA GEILTPDEVA KRLAAANGI
Length:999
Mass (Da):111,551
Last modified:June 1, 2001 - v2
Checksum:iE29C6594AC07D662
GO
Isoform A (identifier: P15330-2) [UniParc]FASTAAdd to basket
Also known as: B

The sequence of this isoform differs from the canonical sequence as follows:
     330-677: GKHTFWNLHR...NSQARKPETP → DPAHLRRKRQ...LQISNLSIST
     678-999: Missing.

Note: Nuclear localization signal at positions 335-340.Curated
Show »
Length:677
Mass (Da):75,347
Checksum:i10FEFCEB1D3A5789
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti236T → S in AAT94434 (Ref. 6) Curated1
Sequence conflicti391H → Q in AAC35296 (PubMed:10072776).Curated1
Sequence conflicti401L → F in AAC35296 (PubMed:10072776).Curated1
Sequence conflicti407V → SQQEQYTQEQSL in AAC35296 (PubMed:10072776).Curated1
Sequence conflicti698 – 699DK → EQ in AAC35296 (PubMed:10072776).Curated2
Sequence conflicti965 – 999ASEFD…AANGI → PVNLTRPPPTMLPWMLARF in AAC35296 (PubMed:10072776).CuratedAdd BLAST35
Isoform A (identifier: P15330-2)
Sequence conflicti506Q → QQ in AAA28479 (PubMed:3118464).Curated1

Alternative sequence

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Alternative sequenceiVSP_005581330 – 677GKHTF…KPETP → DPAHLRRKRQKTGGDPMHLL LQQQQKQQLQNDHQDGRQTN MNCWNTQNIPPIKTEPRDTS PQPFGLSYRAPPELTPSPQP LSPSSNYNHNSTPSPYNMAS AVTPTNGQQQLMSPNHPQQQ QQQQQYGATDLGSNYNPFAQ QVLAQQQQHQQQQQQHQHQH QQQHQQQQQQQQQQQQQSLQ FHANPFGNPGGNSWESKFSA AAVAAAAATATGAAPANGNS NNLSNLNNPFTMHNLLTSGG GPGNANNLQWNLTTNHLHNQ HTLHQQQQLQQQQQQQYDNT APTNNNANLNNNNNNNNTAG NQADNNGPTLSNLLSFDSGQ LVHINSEDQQILRLNSEDLQ ISNLSIST in isoform A. 3 PublicationsAdd BLAST348
Alternative sequenceiVSP_005582678 – 999Missing in isoform A. 3 PublicationsAdd BLAST322

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
M23702 mRNA. Translation: AAA28479.1.
AF053614 mRNA. Translation: AAC35296.1.
AE014134 Genomic DNA. Translation: AAF53611.1.
AE014134 Genomic DNA. Translation: AAF53612.1.
BT015205 mRNA. Translation: AAT94434.1.
PIRiA30350.
RefSeqiNP_001163000.1. NM_001169529.1. [P15330-2]
NP_001163001.1. NM_001169530.1. [P15330-2]
NP_001286014.1. NM_001299085.1. [P15330-1]
NP_724052.1. NM_165217.3. [P15330-2]
NP_724053.1. NM_165218.3. [P15330-2]
NP_724054.1. NM_165219.2. [P15330-1]
UniGeneiDm.3233.

Genome annotation databases

EnsemblMetazoaiFBtr0081007; FBpp0080560; FBgn0260632. [P15330-1]
FBtr0340250; FBpp0309222; FBgn0260632. [P15330-1]
GeneIDi35047.
KEGGidme:Dmel_CG6667.
UCSCiCG6667-RA. d. melanogaster. [P15330-1]

Keywords - Coding sequence diversityi

Alternative splicing

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
M23702 mRNA. Translation: AAA28479.1.
AF053614 mRNA. Translation: AAC35296.1.
AE014134 Genomic DNA. Translation: AAF53611.1.
AE014134 Genomic DNA. Translation: AAF53612.1.
BT015205 mRNA. Translation: AAT94434.1.
PIRiA30350.
RefSeqiNP_001163000.1. NM_001169529.1. [P15330-2]
NP_001163001.1. NM_001169530.1. [P15330-2]
NP_001286014.1. NM_001299085.1. [P15330-1]
NP_724052.1. NM_165217.3. [P15330-2]
NP_724053.1. NM_165218.3. [P15330-2]
NP_724054.1. NM_165219.2. [P15330-1]
UniGeneiDm.3233.

3D structure databases

ProteinModelPortaliP15330.
SMRiP15330.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi61043. 52 interactors.
DIPiDIP-17423N.
IntActiP15330. 5 interactors.
STRINGi7227.FBpp0080560.

PTM databases

iPTMnetiP15330.

Proteomic databases

PaxDbiP15330.
PRIDEiP15330.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblMetazoaiFBtr0081007; FBpp0080560; FBgn0260632. [P15330-1]
FBtr0340250; FBpp0309222; FBgn0260632. [P15330-1]
GeneIDi35047.
KEGGidme:Dmel_CG6667.
UCSCiCG6667-RA. d. melanogaster. [P15330-1]

Organism-specific databases

CTDi35047.
FlyBaseiFBgn0260632. dl.

Phylogenomic databases

eggNOGiENOG410IG8F. Eukaryota.
ENOG410XT64. LUCA.
GeneTreeiENSGT00500000044765.
InParanoidiP15330.
KOiK09254.
OMAiREPSIGH.
OrthoDBiEOG091G07RW.
PhylomeDBiP15330.

Enzyme and pathway databases

ReactomeiR-DME-1169091. Activation of NF-kappaB in B cells.
R-DME-1810476. RIP-mediated NFkB activation via ZBP1.
R-DME-202424. Downstream TCR signaling.
R-DME-209560. NF-kB is activated and signals survival.
R-DME-2871837. FCERI mediated NF-kB activation.
R-DME-3134963. DEx/H-box helicases activate type I IFN and inflammatory cytokines production.
R-DME-445989. TAK1 activates NFkB by phosphorylation and activation of IKKs complex.
R-DME-5607761. Dectin-1 mediated noncanonical NF-kB signaling.
R-DME-5607764. CLEC7A (Dectin-1) signaling.
R-DME-5621575. CD209 (DC-SIGN) signaling.
R-DME-5676590. NIK-->noncanonical NF-kB signaling.
R-DME-933542. TRAF6 mediated NF-kB activation.
SignaLinkiP15330.

Miscellaneous databases

ChiTaRSiDl. fly.
GenomeRNAii35047.
PROiP15330.

Gene expression databases

BgeeiFBgn0260632.
ExpressionAtlasiP15330. baseline.
GenevisibleiP15330. DM.

Family and domain databases

CDDicd01177. IPT_NFkappaB. 1 hit.
Gene3Di2.60.40.10. 1 hit.
2.60.40.340. 1 hit.
InterProiIPR013783. Ig-like_fold.
IPR014756. Ig_E-set.
IPR002909. IPT.
IPR033926. IPT_NFkappaB.
IPR000451. NFkB/Dor.
IPR008967. p53-like_TF_DNA-bd.
IPR030492. RHD_CS.
IPR032397. RHD_dimer.
IPR011539. RHD_DNA_bind_dom.
[Graphical view]
PANTHERiPTHR24169. PTHR24169. 2 hits.
PfamiPF16179. RHD_dimer. 1 hit.
PF00554. RHD_DNA_bind. 1 hit.
[Graphical view]
PRINTSiPR00057. NFKBTNSCPFCT.
SMARTiSM00429. IPT. 1 hit.
[Graphical view]
SUPFAMiSSF49417. SSF49417. 1 hit.
SSF81296. SSF81296. 1 hit.
PROSITEiPS01204. REL_1. 1 hit.
PS50254. REL_2. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiDORS_DROME
AccessioniPrimary (citable) accession number: P15330
Secondary accession number(s): O77088
, Q0E8P9, Q6AWP3, Q9VJD9, Q9VJE0
Entry historyi
Integrated into UniProtKB/Swiss-Prot: April 1, 1990
Last sequence update: June 1, 2001
Last modified: November 30, 2016
This is version 190 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programDrosophila annotation project

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Drosophila
    Drosophila: entries, gene names and cross-references to FlyBase
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.