Skip Header

You are using a version of browser that may not display all the features of this website. Please consider upgrading your browser.
Protein

Putative endoglucanase X

Gene

celX

Organism
Clostridium thermocellum (Ruminiclostridium thermocellum)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Experimental evidence at protein leveli

Functioni

This enzyme catalyzes the endohydrolysis of 1,4-beta-glucosidic linkages in cellulose, lichenin and cereal beta-D-glucans.

Catalytic activityi

Endohydrolysis of (1->4)-beta-D-glucosidic linkages in cellulose, lichenin and cereal beta-D-glucans.

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Glycosidase, Hydrolase

Keywords - Biological processi

Carbohydrate metabolism, Cellulose degradation, Polysaccharide degradation

Names & Taxonomyi

Protein namesi
Recommended name:
Putative endoglucanase X (EC:3.2.1.4)
Short name:
EGX
Alternative name(s):
Cellulase
Endo-1,4-beta-glucanase
Gene namesi
Name:celX
OrganismiClostridium thermocellum (Ruminiclostridium thermocellum)
Taxonomic identifieri1515 [NCBI]
Taxonomic lineageiBacteriaFirmicutesClostridiaClostridialesRuminococcaceaeRuminiclostridium

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini‹1 – 224›224Putative endoglucanase XPRO_0000184076Add
BLAST

Interactioni

Protein-protein interaction databases

STRINGi203119.Cthe_0798.

Structurei

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
2VPTX-ray1.40A9-149[»]
ProteinModelPortaliP15329.
SMRiP15329. Positions 9-146.
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiP15329.

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Repeati168 – 191241Add
BLAST
Repeati202 – 224232Add
BLAST

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni168 – 224572 X 24 AA approximate repeatsAdd
BLAST

Domaini

A 24 residue domain is repeated twice in this enzyme as well as in other C.thermocellum cellulosome enzymes. This domain may function as the binding ligand for the SL component.

Keywords - Domaini

Repeat

Family and domain databases

Gene3Di1.10.1330.10. 1 hit.
3.40.50.1110. 1 hit.
InterProiIPR016134. Cellulos_enz_dockerin_1.
IPR002105. Cellulos_enz_dockerin_1_Ca-bd.
IPR018242. Dockerin_1.
IPR013830. SGNH_hydro.
[Graphical view]
PfamiPF00404. Dockerin_1. 2 hits.
[Graphical view]
SUPFAMiSSF52266. SSF52266. 1 hit.
SSF63446. SSF63446. 1 hit.
PROSITEiPS00448. CLOS_CELLULOSOME_RPT. 2 hits.
PS00018. EF_HAND_1. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Fragment.

P15329-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
IMKVTQDGSI PQIASNINNW LNTHNPDVVF LWIGGNDLLL SGNVNATGLS
60 70 80 90 100
NLIDQIFTVK PNVTLFVADY YPWPEAVKQY NAVIPGIVQQ KANAGKKVYF
110 120 130 140 150
VKLSEIQFDR NTDISWDGLH LSEIGYTKIA NIWYKYTIDI LKALAGQTQP
160 170 180 190 200
TPSPSPTPTD SPLVKKGDVN LDGQVNSTDF SLLKRYILKV VDINSINVTN
210 220
ADMNNDGNIN STDISILKRI LLRN
Length:224
Mass (Da):24,860
Last modified:April 1, 1990 - v1
Checksum:iF8822C5663FF2E80
GO

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Non-terminal residuei1 – 11

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
M22759 Genomic DNA. Translation: AAA23223.1.
PIRiA30476.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
M22759 Genomic DNA. Translation: AAA23223.1.
PIRiA30476.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
2VPTX-ray1.40A9-149[»]
ProteinModelPortaliP15329.
SMRiP15329. Positions 9-146.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi203119.Cthe_0798.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Miscellaneous databases

EvolutionaryTraceiP15329.

Family and domain databases

Gene3Di1.10.1330.10. 1 hit.
3.40.50.1110. 1 hit.
InterProiIPR016134. Cellulos_enz_dockerin_1.
IPR002105. Cellulos_enz_dockerin_1_Ca-bd.
IPR018242. Dockerin_1.
IPR013830. SGNH_hydro.
[Graphical view]
PfamiPF00404. Dockerin_1. 2 hits.
[Graphical view]
SUPFAMiSSF52266. SSF52266. 1 hit.
SSF63446. SSF63446. 1 hit.
PROSITEiPS00448. CLOS_CELLULOSOME_RPT. 2 hits.
PS00018. EF_HAND_1. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

  1. "Conserved reiterated domains in Clostridium thermocellum endoglucanases are not essential for catalytic activity."
    Hall J., Hazlewood G.P., Barker P.J., Gilbert H.J.
    Gene 69:29-38(1988) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].

Entry informationi

Entry nameiGUNX_CLOTM
AccessioniPrimary (citable) accession number: P15329
Entry historyi
Integrated into UniProtKB/Swiss-Prot: April 1, 1990
Last sequence update: April 1, 1990
Last modified: June 24, 2015
This is version 76 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure

Documents

  1. Glycosyl hydrolases
    Classification of glycosyl hydrolase families and list of entries
  2. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.