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Protein

Bisphosphoglycerate mutase

Gene

Bpgm

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Plays a major role in regulating hemoglobin oxygen affinity by controlling the levels of its allosteric effector 2,3-bisphosphoglycerate (2,3-BPG). Also exhibits mutase (EC 5.4.2.1) and phosphatase (EC 3.1.3.13) activities.

Catalytic activityi

3-phospho-D-glyceroyl phosphate = 2,3-bisphospho-D-glycerate.
2-phospho-D-glycerate = 3-phospho-D-glycerate.
2,3-bisphospho-D-glycerate + H2O = 3-phospho-D-glycerate + phosphate.

Enzyme regulationi

At alkaline pH BPGM favors the synthase reaction; however, at lower pH the phosphatase reaction is dominant. Inhibited by citrate (By similarity).By similarity

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Active sitei11 – 111Tele-phosphohistidine intermediateBy similarity
Binding sitei62 – 621SubstrateBy similarity
Active sitei89 – 891Proton donor/acceptorBy similarity
Binding sitei100 – 1001SubstrateBy similarity
Sitei188 – 1881Transition state stabilizerBy similarity

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Hydrolase, Isomerase

Keywords - Biological processi

Glycolysis

Enzyme and pathway databases

ReactomeiR-MMU-70326. Glucose metabolism.

Names & Taxonomyi

Protein namesi
Recommended name:
Bisphosphoglycerate mutase (EC:5.4.2.4)
Short name:
BPGM
Alternative name(s):
2,3-bisphosphoglycerate mutase, erythrocyte
2,3-bisphosphoglycerate synthase (EC:3.1.3.13, EC:5.4.2.11)
BPG-dependent PGAM
Gene namesi
Name:Bpgm
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
Proteomesi
  • UP000000589 Componenti: Chromosome 6

Organism-specific databases

MGIiMGI:1098242. Bpgm.

Subcellular locationi

GO - Cellular componenti

Complete GO annotation...

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Initiator methionineiRemovedBy similarity
Chaini2 – 259258Bisphosphoglycerate mutasePRO_0000179835Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei2 – 21N-acetylserineBy similarity
Modified residuei122 – 1221PhosphothreonineBy similarity

Keywords - PTMi

Acetylation, Phosphoprotein

Proteomic databases

MaxQBiP15327.
PaxDbiP15327.
PeptideAtlasiP15327.
PRIDEiP15327.

2D gel databases

REPRODUCTION-2DPAGEP15327.

PTM databases

iPTMnetiP15327.
PhosphoSiteiP15327.
SwissPalmiP15327.

Expressioni

Tissue specificityi

Expressed in red blood cells. Expressed in placenta (labyrinthine trophoblasts).2 Publications

Gene expression databases

BgeeiENSMUSG00000038871.
CleanExiMM_BPGM.
ExpressionAtlasiP15327. baseline and differential.
GenevisibleiP15327. MM.

Interactioni

Subunit structurei

Homodimer.

Protein-protein interaction databases

IntActiP15327. 1 interaction.
MINTiMINT-4108068.
STRINGi10090.ENSMUSP00000047393.

Structurei

3D structure databases

ProteinModelPortaliP15327.
SMRiP15327. Positions 2-256.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni10 – 178Substrate bindingBy similarity
Regioni23 – 242Substrate bindingBy similarity
Regioni89 – 924Substrate bindingBy similarity
Regioni116 – 1172Substrate bindingBy similarity
Regioni189 – 1902Substrate bindingBy similarity

Sequence similaritiesi

Phylogenomic databases

eggNOGiKOG0235. Eukaryota.
COG0588. LUCA.
GeneTreeiENSGT00390000016700.
HOGENOMiHOG000221682.
HOVERGENiHBG027528.
InParanoidiP15327.
KOiK01837.
OMAiKEAQNCG.
OrthoDBiEOG091G0GIS.
PhylomeDBiP15327.
TreeFamiTF300007.

Family and domain databases

CDDicd07067. HP_PGM_like. 1 hit.
Gene3Di3.40.50.1240. 1 hit.
HAMAPiMF_01039. PGAM_GpmA. 1 hit.
InterProiIPR013078. His_Pase_superF_clade-1.
IPR029033. His_PPase_superfam.
IPR001345. PG/BPGM_mutase_AS.
IPR005952. Phosphogly_mut1.
[Graphical view]
PANTHERiPTHR11931. PTHR11931. 1 hit.
PfamiPF00300. His_Phos_1. 2 hits.
[Graphical view]
SMARTiSM00855. PGAM. 1 hit.
[Graphical view]
SUPFAMiSSF53254. SSF53254. 1 hit.
TIGRFAMsiTIGR01258. pgm_1. 1 hit.
PROSITEiPS00175. PG_MUTASE. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

P15327-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MSKHKLIILR HGEGQWNKEN RFCSWVDQKL NNDGLEEARN CGRQLKALNF
60 70 80 90 100
EFDLVFTSIL NRSIHTAWLI LEELGQEWVP VESSWRLNER HYGALIGLNR
110 120 130 140 150
EKMALNHGEE QVRLWRRSYN VTPPPIEESH PYFHEIYSDR RYKVCDVPLD
160 170 180 190 200
QLPRSESLKD VLERLLPYWK ERIAPEILKG KSILISAHGN SSRALLKHLE
210 220 230 240 250
GISDEDIINI TLPTGVPILL ELDENLRAVG PHQFLGNQEA IQAAIKKVDD

QGKVKQGKQ
Length:259
Mass (Da):29,978
Last modified:January 23, 2007 - v2
Checksum:i6C2674005B41A0C5
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X13586 mRNA. Translation: CAA31927.1.
AK043412 mRNA. Translation: BAC31541.1.
AK078119 mRNA. Translation: BAC37133.1.
BC004589 mRNA. Translation: AAH04589.1.
CCDSiCCDS19994.1.
PIRiA31585. PMMSBM.
RefSeqiNP_031589.1. NM_007563.4.
UniGeneiMm.282863.

Genome annotation databases

EnsembliENSMUST00000045372; ENSMUSP00000047393; ENSMUSG00000038871.
GeneIDi12183.
KEGGimmu:12183.
UCSCiuc009bhf.1. mouse.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X13586 mRNA. Translation: CAA31927.1.
AK043412 mRNA. Translation: BAC31541.1.
AK078119 mRNA. Translation: BAC37133.1.
BC004589 mRNA. Translation: AAH04589.1.
CCDSiCCDS19994.1.
PIRiA31585. PMMSBM.
RefSeqiNP_031589.1. NM_007563.4.
UniGeneiMm.282863.

3D structure databases

ProteinModelPortaliP15327.
SMRiP15327. Positions 2-256.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

IntActiP15327. 1 interaction.
MINTiMINT-4108068.
STRINGi10090.ENSMUSP00000047393.

PTM databases

iPTMnetiP15327.
PhosphoSiteiP15327.
SwissPalmiP15327.

2D gel databases

REPRODUCTION-2DPAGEP15327.

Proteomic databases

MaxQBiP15327.
PaxDbiP15327.
PeptideAtlasiP15327.
PRIDEiP15327.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSMUST00000045372; ENSMUSP00000047393; ENSMUSG00000038871.
GeneIDi12183.
KEGGimmu:12183.
UCSCiuc009bhf.1. mouse.

Organism-specific databases

CTDi669.
MGIiMGI:1098242. Bpgm.

Phylogenomic databases

eggNOGiKOG0235. Eukaryota.
COG0588. LUCA.
GeneTreeiENSGT00390000016700.
HOGENOMiHOG000221682.
HOVERGENiHBG027528.
InParanoidiP15327.
KOiK01837.
OMAiKEAQNCG.
OrthoDBiEOG091G0GIS.
PhylomeDBiP15327.
TreeFamiTF300007.

Enzyme and pathway databases

ReactomeiR-MMU-70326. Glucose metabolism.

Miscellaneous databases

ChiTaRSiBpgm. mouse.
PROiP15327.
SOURCEiSearch...

Gene expression databases

BgeeiENSMUSG00000038871.
CleanExiMM_BPGM.
ExpressionAtlasiP15327. baseline and differential.
GenevisibleiP15327. MM.

Family and domain databases

CDDicd07067. HP_PGM_like. 1 hit.
Gene3Di3.40.50.1240. 1 hit.
HAMAPiMF_01039. PGAM_GpmA. 1 hit.
InterProiIPR013078. His_Pase_superF_clade-1.
IPR029033. His_PPase_superfam.
IPR001345. PG/BPGM_mutase_AS.
IPR005952. Phosphogly_mut1.
[Graphical view]
PANTHERiPTHR11931. PTHR11931. 1 hit.
PfamiPF00300. His_Phos_1. 2 hits.
[Graphical view]
SMARTiSM00855. PGAM. 1 hit.
[Graphical view]
SUPFAMiSSF53254. SSF53254. 1 hit.
TIGRFAMsiTIGR01258. pgm_1. 1 hit.
PROSITEiPS00175. PG_MUTASE. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiPMGE_MOUSE
AccessioniPrimary (citable) accession number: P15327
Secondary accession number(s): Q543Z6
Entry historyi
Integrated into UniProtKB/Swiss-Prot: April 1, 1990
Last sequence update: January 23, 2007
Last modified: September 7, 2016
This is version 149 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.