P15322 (ALL1_SINAL) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names	Recommended name: Allergen Sin a 1 Alternative name(s): Allergen Sin a I Allergen=Sin a 1 Cleaved into the following 2 chains: Allergen Sin a 1 small chain Allergen Sin a 1 large chain
Organism	Sinapis alba (White mustard) (Brassica hirta)
Taxonomic identifier	3728 [NCBI]
Taxonomic lineage	cellular organisms › Eukaryota › Viridiplantae › Streptophyta › Streptophytina › Embryophyta › Tracheophyta › Euphyllophyta › Spermatophyta › Magnoliophyta › eudicotyledons › core eudicotyledons › rosids › malvids › Brassicales › Brassicaceae › Brassiceae › Sinapis

Protein attributes

Sequence length	145 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	This is a 2S seed storage protein.
Subunit structure	The protein consists of two chains linked by disulfide bonds.
Polymorphism	There seems to be at least 8 variants: 1.0101 (Ref.3), 1.0102/SA2S1 (Ref.1), 1.0103/SA2S2 (Ref.1), 1.0104/SIN1 (Ref.2), 1.0105/SIN2 (Ref.2), 1.0106/SIN3 (Ref.2), 1.0107/SIN4 (Ref.2), and 1.0108/SIN5 (Ref.2).
Allergenic properties	Causes an allergic reaction in human. Causes cabbage allergy.
Sequence similarities	Belongs to the 2S seed storage albumins family.

Ontologies

Keywords
Coding sequence diversity	Polymorphism
Disease	Allergen
Molecular function	Seed storage protein Storage protein
PTM	Disulfide bond
Technical term	Direct protein sequencing
Gene Ontology (GO)
Molecular_function	nutrient reservoir activity Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 39	39	Allergen Sin a 1 small chain		PRO_0000032167
	Propeptide	40 – 54	15			PRO_0000032168
	Chain	55 – 145	91	Allergen Sin a 1 large chain		PRO_0000032169
Natural variations
	Natural variant	6	1	R → G in 1.0101, 1.0104/SIN1, 1.0106/SIN3 and 1.0108/SIN5. Ref.2 Ref.3
	Natural variant	36	1	S → G in 1.0103/SA2S2. Ref.1
	Natural variant	40	1	W → L in 1.0103/SA2S2. Ref.1
	Natural variant	41	1	T → A in 1.0103/SA2S2. Ref.1
	Natural variant	43	1	D → E in 1.0105/SIN2.
	Natural variant	43	1	D → Y in 1.0103/SA2S2. Ref.1
	Natural variant	44	1	D → G in 1.0103/SA2S2 and 1.0105/SIN2. Ref.1
	Natural variant	61	1	R → K in 1.0108/SIN5. Ref.2
	Natural variant	100	1	G → E in 1.0101, 1.0103/SA2S2, 1.0104/SIN1, 1.0105/SIN2 and 1.0107/SIN4. Ref.1 Ref.2 Ref.3
	Natural variant	108	1	H → Q in 1.0106/SIN3 and 1.0108/SIN5.
	Natural variant	109	1	L → V in 1.0106/SIN3 and 1.0108/SIN5.
	Natural variant	111	1	H → Q in 1.0103/SA2S2. Ref.1 Ref.2
	Natural variant	125	1	K → R in 1.0104/SIN1. Ref.2
	Natural variant	130	1	R → P in 1.0101, 1.0105/SIN2, 1.0106/SIN3, 1.0107/SIN4 and 1.0108/SIN5. Ref.2 Ref.3
	Natural variant	138	1	K → Q in 1.0104/SIN1. Ref.2
Experimental info
	Sequence conflict	108 – 110	3	Missing AA sequence Ref.3

Sequences

	Sequence		Length	Mass (Da)
	P15322 [UniParc]. Last modified August 16, 2004. Version 2. Checksum: F8EE8E5CC2C2DDA7	FASTA	145	16,449
	10 20 30 40 50 60 PAGPFRIPKC RKEFQQAQHL RACQQWLHKQ AMQSGSGPSW TLDDEFDFED DMENPQGPQQ 70 80 90 100 110 120 RPPLLQQCCN ELHQEEPLCV CPTLKGASKA VKQQVRQQLG QQGQQGPHLQ HVISRIYQTA 130 140 THLPKVCNIR QVSVCPFKKT MPGPS

References

[1]	"Cloning and expression of the major allergen from yellow mustard seeds, Sin a I." Gonzalez de la Pena M.A., Villalba M., Garcia-Lopez J.L., Rodriguez R. Biochem. Biophys. Res. Commun. 190:648-653(1993) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA], VARIANTS GLY-36; LEU-40; ALA-41; TYR-43; GLY-44; GLU-100 AND GLN-111.
[2]	"Expression in Escherichia coli of the major allergen from mustard, Sin a 1." Gonzalez de la Peya M.A., Monsalve R.I., Batanero E., Villalba M., Rodriguez R. Eur. J. Biochem. 237:827-832(1996) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA], VARIANTS GLY-6; 43-GLU-GLY-44; LYS-61; GLU-100; 108-GLN-VAL-109; GLN-111; ARG-125; PRO-130 AND GLN-138. Tissue: Seed.
[3]	"Primary structure of the major allergen of yellow mustard (Sinapis alba L.) seed, Sin a I." Menendez-Arias L., Moneo I., Dominguez J., Rodriguez R. Eur. J. Biochem. 177:159-166(1988) [PubMed] [Europe PMC] [Abstract] Cited for: PROTEIN SEQUENCE OF 1-39 AND 55-145, VARIANTS GLY-6; GLU-100 AND PRO-130. Tissue: Seed.

Cross-references

Sequence databases
EMBL GenBank DDBJ	S54101 Genomic DNA. Translation: AAB25214.2. X91798 Genomic DNA. Translation: CAA62908.1. X91799 Genomic DNA. Translation: CAA62909.1. X91800 Genomic DNA. Translation: CAA62910.1. X91801 Genomic DNA. Translation: CAA62911.1. X91802 Genomic DNA. Translation: CAA62912.1.
PIR	PC1246. PC1247. S01791. S65447. S65478. S65479. S65480. S65481. S65482.
3D structure databases
ProteinModelPortal	P15322.
ModBase	Search...
Protein family/group databases
Allergome	3477. Sin a 1.0101. 627. Sin a 1.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Family and domain databases
InterPro	IPR016140. Bifunc_inhib/LTP/seed_store. IPR000617. Napin. [Graphical view]
Pfam	PF00234. Tryp_alpha_amyl. 1 hit. [Graphical view]
PRINTS	PR00496. NAPIN.
SMART	SM00499. AAI. 1 hit. [Graphical view]
SUPFAM	SSF47699. Bifunc_inhib/LTP/seed_store. 1 hit.
ProtoNet	Search...

Entry information

Entry name

ALL1_SINAL

Accession

Primary (citable) accession number: P15322
Secondary accession number(s): Q41196 , Q41277, Q41278, Q41279, Q41280, Q41281

Entry history

Integrated into UniProtKB/Swiss-Prot:	April 1, 1990
Last sequence update:	August 16, 2004
Last modified:	May 1, 2013
This is version 73 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Plant Protein Annotation Program