P15315 (CUP2_YEAST) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Transcriptional activator protein CUP2

Alternative name(s):
Copper-fist transcription factor

Gene names

Name:	CUP2
Synonyms:	ACE1
Ordered Locus Names:	YGL166W
ORF Names:	G1810

Organism

Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) [Reference proteome]

Taxonomic identifier

559292 [NCBI]

Taxonomic lineage

cellular organisms › Eukaryota › Opisthokonta › Fungi › Dikarya › Ascomycota › saccharomyceta › Saccharomycotina › Saccharomycetes › Saccharomycetales › Saccharomycetaceae › Saccharomyces › Saccharomyces cerevisiae

Protein attributes

Sequence length	225 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	Trans-acting regulatory protein that activates transcription of the CUP1 gene (metallothionein) in response to copper ions. Binds to the CUP1 UAS sequence 5'-GCTTCTTTTCCGCTGA-3'. Binds DNA only in presence of copper or silver. Copper seems to alter the conformation of the protein.
Subcellular location	Nucleus.
Miscellaneous	Present with 1760 molecules/cell in log phase SD medium.
Sequence similarities	Contains 1 copper-fist DNA-binding domain.

Ontologies

Keywords
Biological process	Transcription Transcription regulation
Cellular component	Nucleus
Ligand	Copper DNA-binding Metal-binding Zinc
Molecular function	Activator
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Cellular_component	nucleus Inferred from direct assay Ref.2. Source: SGD
Molecular_function	RNA polymerase II core promoter proximal region sequence-specific DNA binding Inferred from direct assay PubMed 2653812Ref.7Ref.1. Source: SGD copper ion binding Inferred from direct assay PubMed 2068093. Source: SGD copper ion regulated core promoter proximal region sequence-specific DNA binding RNA polymerase II transcription factor activity Inferred from mutant phenotype Ref.1. Source: SGD
Complete GO annotation...

Binary interactions

With	Entry	#Exp.	IntAct	Notes
SRP1	Q02821	1	EBI-2065,EBI-1797

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 225	225	Transcriptional activator protein CUP2		PRO_0000194926
Regions
	DNA binding	1 – 40	40	Copper-fist
	Region	1 – 108	108	Binds copper and DNA
	Region	109 – 225	117	Required for transcriptional activation
Sites
	Metal binding	11	1	Zinc By similarity
	Metal binding	14	1	Zinc By similarity
	Metal binding	23	1	Zinc By similarity
	Metal binding	25	1	Zinc By similarity

Sequences

	Sequence		Length	Mass (Da)
	P15315 [UniParc]. Last modified April 1, 1990. Version 1. Checksum: E3199A3C8CA9CC1B	FASTA	225	24,425
	10 20 30 40 50 60 MVVINGVKYA CETCIRGHRA AQCTHTDGPL QMIRRKGRPS TTCGHCKELR RTKNFNPSGG 70 80 90 100 110 120 CMCASARRPA VGSKEDETRC RCDEGEPCKC HTKRKSSRKS KGGSCHRRAN DEAAHVNGLG 130 140 150 160 170 180 IADLDVLLGL NGRSSDVDMT TTLPSLKPPL QNGEIKADSI DNLDLASLDP LEQSPSISME 190 200 210 220 PVSINETGSA YTTTNTALND IDIPFSINEL NELYKQVSSH NSHSQ

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Copper activates metallothionein gene transcription by altering the conformation of a specific DNA binding protein." Fuerst P., Hu S., Hackett R., Hamer D. Cell 55:705-717(1988) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
[2]	"A cysteine-rich nuclear protein activates yeast metallothionein gene transcription." Szczypka M.S., Thiele D.J. Mol. Cell. Biol. 9:421-429(1989) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
[3]	"DNA sequence analysis of a 35 kb segment from Saccharomyces cerevisiae chromosome VII reveals 19 open reading frames including RAD54, ACE1/CUP2, PMR1, RCK1, AMS1 and CAL1/CDC43." James C.M., Indge K.J., Oliver S.G. Yeast 11:1413-1419(1995) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
[4]	"The nucleotide sequence of Saccharomyces cerevisiae chromosome VII." Tettelin H., Agostoni-Carbone M.L., Albermann K., Albers M., Arroyo J., Backes U., Barreiros T., Bertani I., Bjourson A.J., Brueckner M., Bruschi C.V., Carignani G., Castagnoli L., Cerdan E., Clemente M.L., Coblenz A., Coglievina M., Coissac E. , Defoor E., Del Bino S., Delius H., Delneri D., de Wergifosse P., Dujon B., Durand P., Entian K.-D., Eraso P., Escribano V., Fabiani L., Fartmann B., Feroli F., Feuermann M., Frontali L., Garcia-Gonzalez M., Garcia-Saez M.I., Goffeau A., Guerreiro P., Hani J., Hansen M., Hebling U., Hernandez K., Heumann K., Hilger F., Hofmann B., Indge K.J., James C.M., Klima R., Koetter P., Kramer B., Kramer W., Lauquin G., Leuther H., Louis E.J., Maillier E., Marconi A., Martegani E., Mazon M.J., Mazzoni C., McReynolds A.D.K., Melchioretto P., Mewes H.-W., Minenkova O., Mueller-Auer S., Nawrocki A., Netter P., Neu R., Nombela C., Oliver S.G., Panzeri L., Paoluzi S., Plevani P., Portetelle D., Portillo F., Potier S., Purnelle B., Rieger M., Riles L., Rinaldi T., Robben J., Rodrigues-Pousada C., Rodriguez-Belmonte E., Rodriguez-Torres A.M., Rose M., Ruzzi M., Saliola M., Sanchez-Perez M., Schaefer B., Schaefer M., Scharfe M., Schmidheini T., Schreer A., Skala J., Souciet J.-L., Steensma H.Y., Talla E., Thierry A., Vandenbol M., van der Aart Q.J.M., Van Dyck L., Vanoni M., Verhasselt P., Voet M., Volckaert G., Wambutt R., Watson M.D., Weber N., Wedler E., Wedler H., Wipfli P., Wolf K., Wright L.F., Zaccaria P., Zimmermann M., Zollner A., Kleine K. Nature 387:81-84(1997) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 96604 / S288c / FY1679.
[5]	Saccharomyces Genome Database Submitted (DEC-2009) to the EMBL/GenBank/DDBJ databases Cited for: GENOME REANNOTATION. Strain: ATCC 204508 / S288c.
[6]	"Approaching a complete repository of sequence-verified protein-encoding clones for Saccharomyces cerevisiae." Hu Y., Rolfs A., Bhullar B., Murthy T.V.S., Zhu C., Berger M.F., Camargo A.A., Kelley F., McCarron S., Jepson D., Richardson A., Raphael J., Moreira D., Taycher E., Zuo D., Mohr S., Kane M.F., Williamson J. , Simpson A.J.G., Bulyk M.L., Harlow E., Marsischky G., Kolodner R.D., LaBaer J. Genome Res. 17:536-543(2007) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Strain: ATCC 204508 / S288c.
[7]	"The CUP2 gene product, regulator of yeast metallothionein expression, is a copper-activated DNA-binding protein." Buchman C., Skroch P., Welch J., Fogel S., Karin M. Mol. Cell. Biol. 9:4091-4095(1989) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 1-48.
[8]	Klima R., Coglievina M., Zaccaria P., Bertani I., Bruschi C.V. Submitted (MAR-1995) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 1-33. Strain: ATCC 96604 / S288c / FY1679.
[9]	"Regulation of metallothionein genes by the ACE1 and AMT1 transcription factors." Thorvaldsen J.L., Sewell A.K., McCowen C.L., Winge D.R. J. Biol. Chem. 268:12512-12518(1993) [PubMed] [Europe PMC] [Abstract] Cited for: CHARACTERIZATION.
[10]	"Global analysis of protein expression in yeast." Ghaemmaghami S., Huh W.-K., Bower K., Howson R.W., Belle A., Dephoure N., O'Shea E.K., Weissman J.S. Nature 425:737-741(2003) [PubMed] [Europe PMC] [Abstract] Cited for: LEVEL OF PROTEIN EXPRESSION [LARGE SCALE ANALYSIS].
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	M22580 Genomic DNA. Translation: AAA66313.1. M24390 Genomic DNA. Translation: AAA34386.1. Z48618 Genomic DNA. Translation: CAA88533.1. X85757 Genomic DNA. Translation: CAA59765.1. M28520 Genomic DNA. Translation: AAA34542.1. Z72688 Genomic DNA. Translation: CAA96877.1. AY557820 Genomic DNA. Translation: AAS56146.1. BK006941 Genomic DNA. Translation: DAA07947.1.
PIR	A31926.
RefSeq	NP_011349.3. NM_001181031.3.
3D structure databases
ProteinModelPortal	P15315.
SMR	P15315. Positions 1-42.
ModBase	Search...
Protein-protein interaction databases
DIP	DIP-1335N.
IntAct	P15315. 9 interactions.
MINT	MINT-384247.
STRING	4932.YGL166W.
Proteomic databases
PaxDb	P15315.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblFungi	YGL166W; YGL166W; YGL166W.
GeneID	852710.
KEGG	sce:YGL161C. sce:YGL166W.
Organism-specific databases
CYGD	YGL166w.
SGD	S000003134. CUP2.
Phylogenomic databases
eggNOG	NOG71500.
GeneTree	ENSGT00530000068810.
KO	K09281.
OrthoDB	EOG4X0R35.
Gene expression databases
Genevestigator	P15315.
GermOnline	YGL166W. Saccharomyces cerevisiae.
Family and domain databases
Gene3D	3.90.430.10. 1 hit.
InterPro	IPR001083. Cu_fist_DNA-bd. [Graphical view]
Pfam	PF00649. Copper-fist. 1 hit. [Graphical view]
PRINTS	PR00617. COPPERFIST.
SMART	SM00412. Cu_FIST. 1 hit. [Graphical view]
SUPFAM	SSF57879. Cu_fist_DNA_bd. 1 hit.
PROSITE	PS01119. COPPER_FIST_1. 1 hit. PS50073. COPPER_FIST_2. 1 hit. [Graphical view]
ProtoNet	Search...
Other
NextBio	972073.

Entry information

Entry name

CUP2_YEAST

Accession

Primary (citable) accession number: P15315
Secondary accession number(s): D6VTY6

Entry history

Integrated into UniProtKB/Swiss-Prot:	April 1, 1990
Last sequence update:	April 1, 1990
Last modified:	April 3, 2013
This is version 117 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Fungal Protein Annotation Program