Reviewed, UniProtKB/Swiss-Prot P15315 (ACE1_YEAST)

Names and origin

Protein names

Recommended name:
    Transcriptional activator protein ACE1
Alternative name(s):
    Copper-fist transcription factor

Gene names

Name:	ACE1
Synonyms:	CUP2
Ordered Locus Names:	YGL166W
ORF Names:	G1810

Organism

Saccharomyces cerevisiae (Baker's yeast) [Complete proteome]

Taxonomic identifier

4932 [NCBI]

Taxonomic lineage

Eukaryota › Fungi › Dikarya › Ascomycota › Saccharomycotina › Saccharomycetes › Saccharomycetales › Saccharomycetaceae › Saccharomyces

Protein attributes

Sequence length	225 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Evidence at protein level.

General annotation (Comments)

Function	Trans-acting regulatory protein that activates transcription of the CUP1 gene (metallothionein) in response to copper ions. Binds to the CUP1 UAS sequence 5'-GCTTCTTTTCCGCTGA-3'. Binds DNA only in presence of copper or silver. Copper seems to alter the conformation of the protein.
Subcellular location	Nucleus.
Miscellaneous	Present with 1760 molecules/cell in log phase SD medium. Ref.9
Sequence similarities	Contains 1 copper-fist DNA-binding domain.

Ontologies

Keywords
Biological process	Transcription Transcription regulation
Cellular component	Nucleus
Ligand	Copper DNA-binding Metal-binding Zinc
Molecular function	Activator
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	regulation of transcription, DNA-dependent Inferred from electronic annotation. Source: InterPro response to copper ion Inferred from mutant phenotype. Source: SGD transcription initiation from RNA polymerase II promoter Ref.6 Inferred from mutant phenotype. Source: SGD
Cellular component	nucleus Ref.2 Inferred from direct assay. Source: SGD
Molecular function	copper ion binding Inferred from electronic annotation. Source: UniProtKB-KW ligand-regulated transcription factor activity Ref.6 Inferred from mutant phenotype. Source: SGD protein binding Inferred from physical interaction. Source: IntAct transcription factor activity Inferred from electronic annotation. Source: InterPro zinc ion binding Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Binary interactions

With	Entry	#Exp.	IntAct	Notes
SRP1	Q02821	1	EBI-2065,EBI-1797

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 225	225	Transcriptional activator protein ACE1		PRO_0000194926
Regions
	DNA binding	1 – 40	40	Copper-fist
	Region	1 – 108	108	Binds copper and DNA
	Region	109 – 225	117	Required for transcriptional activation
Sites
	Metal binding	11	1	Zinc By similarity
	Metal binding	14	1	Zinc By similarity
	Metal binding	23	1	Zinc By similarity
	Metal binding	25	1	Zinc By similarity

Sequences

	Sequence		Length	Mass (Da)
	P15315-1 [UniParc]. Last modified April 1, 1990. Version 1. Checksum: E3199A3C8CA9CC1B	FASTA	225	24,425
	10 20 30 40 50 60 MVVINGVKYA CETCIRGHRA AQCTHTDGPL QMIRRKGRPS TTCGHCKELR RTKNFNPSGG 70 80 90 100 110 120 CMCASARRPA VGSKEDETRC RCDEGEPCKC HTKRKSSRKS KGGSCHRRAN DEAAHVNGLG 130 140 150 160 170 180 IADLDVLLGL NGRSSDVDMT TTLPSLKPPL QNGEIKADSI DNLDLASLDP LEQSPSISME 190 200 210 220 PVSINETGSA YTTTNTALND IDIPFSINEL NELYKQVSSH NSHSQ

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Copper activates metallothionein gene transcription by altering the conformation of a specific DNA binding protein." Fuerst P., Hu S., Hackett R., Hamer D. Cell 55:705-717(1988) [PubMed: 3052856] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
[2]	"A cysteine-rich nuclear protein activates yeast metallothionein gene transcription." Szczypka M.S., Thiele D.J. Mol. Cell. Biol. 9:421-429(1989) [PubMed: 2651899] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
[3]	"DNA sequence analysis of a 35 kb segment from Saccharomyces cerevisiae chromosome VII reveals 19 open reading frames including RAD54, ACE1/CUP2, PMR1, RCK1, AMS1 and CAL1/CDC43." James C.M., Indge K.J., Oliver S.G. Yeast 11:1413-1419(1995) [PubMed: 8585324] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
[4]	"The nucleotide sequence of Saccharomyces cerevisiae chromosome VII." Tettelin H., Agostoni-Carbone M.L., Albermann K., Albers M., Arroyo J., Backes U., Barreiros T., Bertani I., Bjourson A.J., Brueckner M., Bruschi C.V., Carignani G., Castagnoli L., Cerdan E., Clemente M.L., Coblenz A., Coglievina M., Coissac E. , Defoor E., Del Bino S., Delius H., Delneri D., de Wergifosse P., Dujon B., Durand P., Entian K.-D., Eraso P., Escribano V., Fabiani L., Fartmann B., Feroli F., Feuermann M., Frontali L., Garcia-Gonzalez M., Garcia-Saez M.I., Goffeau A., Guerreiro P., Hani J., Hansen M., Hebling U., Hernandez K., Heumann K., Hilger F., Hofmann B., Indge K.J., James C.M., Klima R., Koetter P., Kramer B., Kramer W., Lauquin G., Leuther H., Louis E.J., Maillier E., Marconi A., Martegani E., Mazon M.J., Mazzoni C., McReynolds A.D.K., Melchioretto P., Mewes H.-W., Minenkova O., Mueller-Auer S., Nawrocki A., Netter P., Neu R., Nombela C., Oliver S.G., Panzeri L., Paoluzi S., Plevani P., Portetelle D., Portillo F., Potier S., Purnelle B., Rieger M., Riles L., Rinaldi T., Robben J., Rodrigues-Pousada C., Rodriguez-Belmonte E., Rodriguez-Torres A.M., Rose M., Ruzzi M., Saliola M., Sanchez-Perez M., Schaefer B., Schaefer M., Scharfe M., Schmidheini T., Schreer A., Skala J., Souciet J.-L., Steensma H.Y., Talla E., Thierry A., Vandenbol M., van der Aart Q.J.M., Van Dyck L., Vanoni M., Verhasselt P., Voet M., Volckaert G., Wambutt R., Watson M.D., Weber N., Wedler E., Wedler H., Wipfli P., Wolf K., Wright L.F., Zaccaria P., Zimmermann M., Zollner A., Kleine K. Nature 387:81-84(1997) [PubMed: 9169869] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 96604 / S288c / FY1679.
[5]	"Approaching a complete repository of sequence-verified protein-encoding clones for Saccharomyces cerevisiae." Hu Y., Rolfs A., Bhullar B., Murthy T.V.S., Zhu C., Berger M.F., Camargo A.A., Kelley F., McCarron S., Jepson D., Richardson A., Raphael J., Moreira D., Taycher E., Zuo D., Mohr S., Kane M.F., Williamson J. , Simpson A.J.G., Bulyk M.L., Harlow E., Marsischky G., Kolodner R.D., LaBaer J. Genome Res. 17:536-543(2007) [PubMed: 17322287] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Strain: ATCC 204508 / S288c.
[6]	"The CUP2 gene product, regulator of yeast metallothionein expression, is a copper-activated DNA-binding protein." Buchman C., Skroch P., Welch J., Fogel S., Karin M. Mol. Cell. Biol. 9:4091-4095(1989) [PubMed: 2674688] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 1-48.
[7]	Klima R., Coglievina M., Zaccaria P., Bertani I., Bruschi C.V. Submitted (MAR-1995) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 1-33. Strain: ATCC 96604 / S288c / FY1679.
[8]	"Regulation of metallothionein genes by the ACE1 and AMT1 transcription factors." Thorvaldsen J.L., Sewell A.K., McCowen C.L., Winge D.R. J. Biol. Chem. 268:12512-12518(1993) [PubMed: 8509391] [Abstract] Cited for: CHARACTERIZATION.
[9]	"Global analysis of protein expression in yeast." Ghaemmaghami S., Huh W.-K., Bower K., Howson R.W., Belle A., Dephoure N., O'Shea E.K., Weissman J.S. Nature 425:737-741(2003) [PubMed: 14562106] [Abstract] Cited for: LEVEL OF PROTEIN EXPRESSION [LARGE SCALE ANALYSIS].
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBLGenBankDDBJ	M22580 Genomic DNA. Translation: AAA66313.1. M24390 Genomic DNA. Translation: AAA34386.1. Z48618 Genomic DNA. Translation: CAA88533.1. X85757 Genomic DNA. Translation: CAA59765.1. M28520 Genomic DNA. Translation: AAA34542.1. Z72688 Genomic DNA. Translation: CAA96877.1. AY557820 Genomic DNA. Translation: AAS56146.1.
PIR	A31926.
RefSeq	NP_011349.1.
3D structure databases
HSSP	HSSP built from PDB template 1CO4 based on UniProtKB P41772.
ModBase	Search...
Protein-protein interaction databases
DIP	DIP:1335N.
IntAct	P15315. 16 interactions.
STRING	P15315.
Genome annotation databases
Ensembl	YGL166W; YGL166W; YGL166W; Saccharomyces cerevisiae. [Genome view]
GeneID	852710.
GenomeReviews	Gene locus YGL166W in contig Y13135_GR.
KEGG	sce:YGL166W.
NMPDR	fig|4932.3.peg.2453.
Organism-specific databases
CYGD	YGL166w.
SGD	S000003134. CUP2.
Phylogenomic databases
HOGENOM	P15315.
Gene expression databases
ArrayExpress	P15315.
Genevestigator	P15315.
GermOnline	YGL166W. Saccharomyces cerevisiae.
Family and domain databases
InterPro	IPR018373. Cu_fist_DNA-bd_CS. IPR001083. Cu_fist_DNA_bd. [Graphical view]
Gene3D	G3DSA:3.90.430.10. Cu_fist_DNA_bd. 1 hit.
Pfam	PF00649. Copper-fist. 1 hit. [Graphical view]
PRINTS	PR00617. COPPERFIST.
ProDom	PD009050. Copper-fist. 1 hit. [Graphical view] [Entries sharing at least one domain]
SMART	SM00412. Cu_FIST. 1 hit. [Graphical view]
PROSITE	PS01119. COPPER_FIST_1. 1 hit. PS50073. COPPER_FIST_2. 1 hit. [Graphical view]
ProtoNet	Search...
Other Resources
NextBio	972073.

Entry information

Entry name

ACE1_YEAST

Accession

Primary (citable) accession number: P15315

Entry history

Integrated into UniProtKB/Swiss-Prot:	April 1, 1990
Last sequence update:	April 1, 1990
Last modified:	October 13, 2009
This is version 83 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

FPAP (Fungal Proteome Annotation Project)