Reviewed,
UniProtKB/Swiss-Prot P15311 (EZRI_HUMAN)
Last modified
July 7, 2009.
Version 112.
History...
Clusters with 100%,
90%,
50% identity |
Documents (6) |
Third-party data |
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Names and origin
| Protein names | Recommended name: Ezrin Alternative name(s): p81 Cytovillin Villin-2 | ||||
| Gene names |
| ||||
| Organism | Homo sapiens (Human) [Complete proteome] | ||||
| Taxonomic identifier | 9606 [NCBI] | ||||
| Taxonomic lineage | Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Primates › Haplorrhini › Catarrhini › Hominidae › Homo |
Protein attributes
| Sequence length | 586 AA. |
| Sequence status | Complete. |
| Sequence processing | The displayed sequence is further processed into a mature form. |
| Protein existence | Evidence at protein level. |
General annotation (Comments)
| Function | Probably involved in connections of major cytoskeletal structures to the plasma membrane. In epithelial cells, required for the formation of microvilli and membrane ruffles on the apical pole. Along with PLEKHG6, required for normal macropinocytosis. Ref.14 |
| Subunit structure | Interacts with MPP5 By similarity. Interacts with SLC9A3R1 and SCYL3/PACE1. Interacts with PLEKHG6. Interacts with NGX6. |
| Subcellular location | Apical cell membrane; Peripheral membrane protein; Cytoplasmic side. Cell projection. Cell projection › microvillus membrane; Peripheral membrane protein; Cytoplasmic side. Cell projection › ruffle membrane; Peripheral membrane protein; Cytoplasmic side. Cytoplasm › cell cortex. Cytoplasm › cytoskeleton. Note: Localization to the apical membrane of parietal cells depends on the interaction with MPP5. Localizes to cell extensions and peripheral processes of astrocytes By similarity. Microvillar peripheral membrane protein (cytoplasmic side). |
| Tissue specificity | Expressed in cerebral cortex, basal ganglia, hippocampus, hypophysis, and optic nerve. Weakly expressed in brain stem and diencephalon. Stronger expression was detected in gray matter of frontal lobe compared to white matter (at protein level). Component of the microvilli of intestinal epithelial cells. Preferentially expressed in astrocytes of hippocampus, frontal cortex, thalamus, parahippocampal cortex, amygdala, insula, and corpus callosum. Not detected in neurons in most tissues studied. Ref.13 |
| Developmental stage | Very strong staining is detected in the Purkinje cell layer and in part of the molecular layer of the infant brain compared to adult brain. Ref.13 |
| Post-translational modification | |
| Sequence similarities | Contains 1 FERM domain. |
Ontologies
Binary interactions
With | Entry | #Exp. | IntAct | Notes |
|---|---|---|---|---|
| GABARAPL2 | P60520 | 1 | EBI-1056902,EBI-720116 | |
| HLA-B | P30480 | 1 | EBI-1056902,EBI-1054175 | |
| MCC | P23508 | 1 | EBI-1056902,EBI-307531 | |
| PRKAB1 | Q9Y478 | 1 | EBI-1056902,EBI-719769 | |
| SCYL3 | Q8IZE3 | 2 | EBI-1056902,EBI-1380680 |
Sequence annotation (Features)
| Feature key | Position(s) | Length | Description | Graphical view | Feature identifier | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Molecule processing | |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
| Initiator methionine | 1 | 1 | Removed | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
| Chain | 2 – 586 | 585 | Ezrin | PRO_0000219408 | |||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Regions | |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
| Domain | 2 – 295 | 294 | FERM | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
| Region | 244 – 586 | 343 | Interaction with SCYL3 | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Amino acid modifications | |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
| Modified residue | 146 | 1 | Phosphotyrosine; by PDGFR | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
| Modified residue | 354 | 1 | Phosphotyrosine; by PDGFR | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Natural variations | |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
| Natural variant | 180 | 1 | R → C: dbSNP rs3103004. | VAR_030572 | |||||||||||||||||||||||||||||||||||||||||||||||||||||||||
| Natural variant | 494 | 1 | A → P: dbSNP rs2230143. | VAR_030573 | |||||||||||||||||||||||||||||||||||||||||||||||||||||||||
| Natural variant | 532 | 1 | L → V Ref.1 Ref.2 | VAR_015112 | |||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Secondary structure | |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Helix Strand Turn | |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
| Beta strand | 5 – 11 | 7 | |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
| Beta strand | 14 – 20 | 7 | |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
| Helix | 26 – 37 | 12 | |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
| Helix | 42 – 44 | 3 | |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
| Beta strand | 45 – 51 | 7 | |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
| Beta strand | 56 – 58 | 3 | |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
| Beta strand | 61 – 64 | 4 | |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
| Helix | 65 – 67 | 3 | |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
| Beta strand | 74 – 84 | 11 | |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
| Helix | 89 – 92 | 4 | |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
| Helix | 96 – 111 | 16 | |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
| Helix | 119 – 134 | 16 | |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
| Turn | 139 – 141 | 3 | |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
| Beta strand | 146 – 149 | 4 | |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
| Helix | 155 – 160 | 6 | |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
| Helix | 165 – 178 | 14 | |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
| Helix | 184 – 195 | 12 | |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
| Turn | 199 – 202 | 4 | |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
| Beta strand | 204 – 210 | 7 | |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
| Beta strand | 215 – 220 | 6 | |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
| Beta strand | 222 – 229 | 8 | |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
| Beta strand | 232 – 235 | 4 | |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
| Beta strand | 237 – 242 | 6 | |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
| Beta strand | 245 – 253 | 9 | |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
| Beta strand | 255 – 262 | 8 | |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
| Beta strand | 268 – 270 | 3 | |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
| Helix | 274 – 293 | 20 | |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Sequences
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References
| « Hide 'large scale' references | |
| [1] | "cDNA cloning and sequencing of the protein-tyrosine kinase substrate, ezrin, reveals homology to band 4.1." Gould K.L., Bretscher A., Esch F.S., Hunter T. EMBO J. 8:4133-4142(1989) [PubMed: 2591371] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA], PARTIAL PROTEIN SEQUENCE, VARIANT VAL-532. |
| [2] | "Cytovillin, a microvillar Mr 75,000 protein. cDNA sequence, prokaryotic expression, and chromosomal localization." Turunen O., Winqvist R., Pakkanen R., Grzeschik K.-H., Wahlstroem T., Vaheri A. J. Biol. Chem. 264:16727-16732(1989) [PubMed: 2674140] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA], VARIANT VAL-532. Tissue: Placenta. |
| [3] | "The full-ORF clone resource of the German cDNA consortium." Bechtel S., Rosenfelder H., Duda A., Schmidt C.P., Ernst U., Wellenreuther R., Mehrle A., Schuster C., Bahr A., Blocker H., Heubner D., Hoerlein A., Michel G., Wedler H., Kohrer K., Ottenwalder B., Poustka A., Wiemann S., Schupp I. BMC Genomics 8:399-399(2007) [PubMed: 17974005] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. Tissue: Melanoma. |
| [4] | "The DNA sequence and analysis of human chromosome 6." Mungall A.J., Palmer S.A., Sims S.K., Edwards C.A., Ashurst J.L., Wilming L., Jones M.C., Horton R., Hunt S.E., Scott C.E., Gilbert J.G.R., Clamp M.E., Bethel G., Milne S., Ainscough R., Almeida J.P., Ambrose K.D., Andrews T.D. Beck S.Nature 425:805-811(2003) [PubMed: 14574404] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. |
| [5] | "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)." The MGC Project Team Genome Res. 14:2121-2127(2004) [PubMed: 15489334] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. Tissue: Colon. |
| [6] | "Identification of the 70kD heat shock cognate protein (Hsc70) and alpha-actinin-1 as novel phosphotyrosine-containing proteins in T lymphocytes." Egerton M., Moritz R.L., Druker B., Kelso A., Simpson R.J. Biochem. Biophys. Res. Commun. 224:666-674(1996) [PubMed: 8713105] [Abstract] Cited for: PROTEIN SEQUENCE OF 172-180 AND 343-350. |
| [7] | Lubec G., Chen W.-Q., Sun Y. Submitted (DEC-2008) to UniProtKB Cited for: PROTEIN SEQUENCE OF 264-273; 428-435 AND 530-542, MASS SPECTROMETRY. Tissue: Fetal brain cortex. |
| [8] | "Identification of the two major epidermal growth factor-induced tyrosine phosphorylation sites in the microvillar core protein ezrin." Krieg J., Hunter T. J. Biol. Chem. 267:19258-19265(1992) [PubMed: 1382070] [Abstract] Cited for: PHOSPHORYLATION BY PDGFR. |
| [9] | "Identification of ezrin as an 81-kDa tyrosine-phosphorylated protein in T cells." Egerton M., Burgess W.H., Chen D., Druker B.J., Bretscher A., Samelson L.E. J. Immunol. 149:1847-1852(1992) [PubMed: 1381389] [Abstract] Cited for: PHOSPHORYLATION. |
| [10] | "Identification of EBP50: a PDZ-containing phosphoprotein that associates with members of the ezrin-radixin-moesin family." Reczek D., Berryman M., Bretscher A. J. Cell Biol. 139:169-179(1997) [PubMed: 9314537] [Abstract] Cited for: INTERACTION WITH SLC9A3R1. |
| [11] | "PACE-1, a novel protein that interacts with the C-terminal domain of ezrin." Sullivan A., Uff C.R., Isacke C.M., Thorne R.F. Exp. Cell Res. 284:224-238(2003) [PubMed: 12651155] [Abstract] Cited for: INTERACTION WITH SCYL3. Tissue: Kidney. |
| [12] | "Role of a novel EGF-like domain-containing gene NGX6 in cell adhesion modulation in nasopharyngeal carcinoma cells." Ma J., Zhou J., Fan S., Wang L.-L., Li X.-L., Yan Q., Zhou M., Liu H.-Y., Zhang Q., Zhou H., Gan K., Li Z., Peng C., Xiong W., Tan C., Shen S.-R., Yang J., Li J., Li G.-Y. Carcinogenesis 26:281-291(2005) [PubMed: 15498789] [Abstract] Cited for: INTERACTION WITH NGX6. |
| [13] | "Characterization of the NF2 protein merlin and the ERM protein ezrin in human, rat, and mouse central nervous system." Groenholm M., Teesalu T., Tyynelaa J., Piltti K., Boehling T., Wartiovaara K., Vaheri A., Carpen O. Mol. Cell. Neurosci. 28:683-693(2005) [PubMed: 15797715] [Abstract] Cited for: TISSUE SPECIFICITY, DEVELOPMENTAL STAGE. |
| [14] | "Interaction of ezrin with the novel guanine nucleotide exchange factor PLEKHG6 promotes RhoG-dependent apical cytoskeleton rearrangements in epithelial cells." D'Angelo R., Aresta S., Blangy A., Del Maestro L., Louvard D., Arpin M. Mol. Biol. Cell 18:4780-4793(2007) [PubMed: 17881735] [Abstract] Cited for: FUNCTION, INTERACTION WITH PLEKHG6. |
| [15] | Colinge J., Superti-Furga G., Bennett K.L. Submitted (OCT-2008) to UniProtKB Cited for: IDENTIFICATION [LARGE SCALE ANALYSIS], MASS SPECTROMETRY. |
| [16] | "Structure of the active N-terminal domain of Ezrin. Conformational and mobility changes identify keystone interactions." Smith W.J., Nassar N., Bretscher A., Cerione R.A., Karplus P.A. J. Biol. Chem. 278:4949-4956(2003) [PubMed: 12429733] [Abstract] Cited for: X-RAY CRYSTALLOGRAPHY (2.3 ANGSTROMS) OF 1-297. |
| + | Additional computationally mapped references. |
Cross-references
Sequence databases | |||||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
| X51521 mRNA. Translation: CAA35893.1. J05021 mRNA. Translation: AAA61278.1. Different initiation. AL162086 mRNA. Translation: CAB82418.1. Different initiation. AL589931 Genomic DNA. Translation: CAI95307.1. BC013903 mRNA. Translation: AAH13903.1. | |||||||||||||
| IPI | IPI00843975. | ||||||||||||
| PIR | A34400. | ||||||||||||
| RefSeq | NP_001104547.1. NP_003370.2. | ||||||||||||
| UniGene | Hs.487027 | ||||||||||||
3D structure databases | |||||||||||||
| |||||||||||||
| ModBase | Search... | ||||||||||||
Protein-protein interaction databases | |||||||||||||
| IntAct | P15311. 14 interactions. | ||||||||||||
PTM databases | |||||||||||||
| PhosphoSite | P15311. | ||||||||||||
2-D gel databases | |||||||||||||
| SWISS-2DPAGE | P15311. | ||||||||||||
| DOSAC-COBS-2DPAGE | P15311. | ||||||||||||
| REPRODUCTION-2DPAGE | IPI00843975. | ||||||||||||
Proteomic databases | |||||||||||||
| PRIDE | P15311. | ||||||||||||
Genome annotation databases | |||||||||||||
| Ensembl | ENSG00000092820. Homo sapiens. [Contig view] | ||||||||||||
| GeneID | 7430. | ||||||||||||
| KEGG | hsa:7430. | ||||||||||||
| UCSC | uc003qrt.2. human. | ||||||||||||
Organism-specific databases | |||||||||||||
| GeneCards | GC06M159106. | ||||||||||||
| HGNC | HGNC:12691. EZR. | ||||||||||||
| HPA | CAB004035. | ||||||||||||
| MIM | 123900. gene. | ||||||||||||
| PharmGKB | PA37310. | ||||||||||||
| GenAtlas | Search... | ||||||||||||
Phylogenomic databases | |||||||||||||
| HOVERGEN | P15311. | ||||||||||||
Enzyme and pathway databases | |||||||||||||
| Pathway_Interaction_DB | syndecan_2_pathway. Syndecan-2-mediated signaling events. | ||||||||||||
Gene expression databases | |||||||||||||
| ArrayExpress | P15311. | ||||||||||||
| Bgee | P15311. | ||||||||||||
| CleanEx | HS_EZR. | ||||||||||||
| GermOnline | ENSG00000092820. Homo sapiens. | ||||||||||||
Family and domain databases | |||||||||||||
| InterPro | IPR019749. Band_41_domain. IPR019750. Band_41_sg. IPR011174. ERM. IPR011259. ERM_C. IPR000798. Ez/rad/moesin. IPR014352. FERM/acyl-CoA_bd_prot_3-hlx. IPR019748. FERM_central. IPR019747. FERM_CS. IPR000299. FERM_domain. IPR018979. FERM_N. IPR018980. FERM_PH-like_C. IPR011993. PH_type. [Graphical view] | ||||||||||||
| Gene3D | G3DSA:1.20.80.10. ACBP. 1 hit. G3DSA:2.30.29.30. PH_type. 1 hit. | ||||||||||||
| Pfam | PF00769. ERM. 1 hit. PF09380. FERM_C. 1 hit. PF00373. FERM_M. 1 hit. PF09379. FERM_N. 1 hit. [Graphical view] | ||||||||||||
| PIRSF | PIRSF002305. ERM. 1 hit. | ||||||||||||
| PRINTS | PR00935. BAND41. PR00661. ERMFAMILY. | ||||||||||||
| SMART | SM00295. B41. 1 hit. [Graphical view] | ||||||||||||
| PROSITE | PS00660. FERM_1. 1 hit. PS00661. FERM_2. 1 hit. PS50057. FERM_3. 1 hit. [Graphical view] | ||||||||||||
| ProtoNet | Search... | ||||||||||||
Other Resources | |||||||||||||
| NextBio | 29100. | ||||||||||||
| SOURCE | Search... | ||||||||||||
Entry information
| Entry name | EZRI_HUMAN | ||||||||
| Accession | Primary (citable) accession number: P15311 Secondary accession number(s): P23714 Q9NSJ4 | ||||||||
| Entry history |
| ||||||||
| Entry status | Reviewed (UniProtKB/Swiss-Prot) | ||||||||
| Annotation project | HPI (Human Proteome Initiative) | ||||||||
Relevant documents
| Human chromosome 6 Human chromosome 6: entries, gene names and cross-references to MIM |
| Human entries with polymorphisms or disease mutations List of human entries with polymorphisms or disease mutations |
| Human polymorphisms and disease mutations Index of human polymorphisms and disease mutations |
| MIM cross-references Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot |
| PDB cross-references Index of Protein Data Bank (PDB) cross-references |
| SIMILARITY comments Index of protein domains and families |

Clusters with


