Reviewed, UniProtKB/Swiss-Prot P15309 (PPAP_HUMAN)

Names and origin

Protein names

Recommended name:
    Prostatic acid phosphatase
    EC=3.1.3.2

Gene names

Name:

ACPP

Organism

Homo sapiens (Human) [Complete proteome]

Taxonomic identifier

9606 [NCBI]

Taxonomic lineage

Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Primates › Haplorrhini › Catarrhini › Hominidae › Homo

Protein attributes

Sequence length	386 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Evidence at protein level.

General annotation (Comments)

Catalytic activity	A phosphate monoester + H2O = an alcohol + phosphate.
Subunit structure	Homodimer.
Subcellular location	Isoform 1: Secreted By similarity. Isoform 2: Lysosome membrane; Single-pass type I membrane protein By similarity. Note: Predominantly localized in the plasma membrane but also detected in intracellular vesicles By similarity.
Sequence similarities	Belongs to the histidine acid phosphatase family.

Ontologies

Keywords
Cellular component	Lysosome Membrane Secreted
Coding sequence diversity	Alternative splicing Polymorphism
Domain	Signal
Molecular function	Hydrolase
PTM	Disulfide bond Glycoprotein
Technical term	3D-structure Complete proteome Direct protein sequencing
Gene Ontology (GO)
Cellular component	extracellular region Inferred from electronic annotation. Source: UniProtKB-SubCell integral to membrane Inferred from electronic annotation. Source: UniProtKB-SubCell lysosome Inferred from electronic annotation. Source: UniProtKB-KW
Molecular function	acid phosphatase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Alternative products

This entry describes 2 isoforms produced by alternative splicing. [Align] [Select]

Isoform 1 (identifier: P15309-1)

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

Isoform 2 (identifier: P15309-2)

The sequence of this isoform differs from the canonical sequence as follows:
     380-386: GTEDSTD → VLKVIFAVAFCLISAVLMVLLFIHIRRGLCWQRESYGNI

Note: Predominantly localized in the plasma membrane but also detected in intracellular vesicles (By similarity).

Sequence annotation (Features)

Feature key

Position(s)

Length

Description

Graphical view

Feature identifier

Molecule processing

Signal peptide

1 – 32

32

Chain

33 – 386

354

Prostatic acid phosphatase

PRO_0000023963

Sites

Active site

44

1

Nucleophile By similarity

Active site

290

1

Proton donor Ref.2

Amino acid modifications

Glycosylation

94

1

N-linked (GlcNAc...)

Glycosylation

220

1

N-linked (GlcNAc...)

Glycosylation

333

1

N-linked (GlcNAc...)

Disulfide bond

161 ↔ 372

Ref.2

Disulfide bond

215 ↔ 313

Ref.2

Disulfide bond

347 ↔ 351

Ref.2

Natural variations

Alternative sequence

380 – 386

7

GTEDSTD → VLKVIFAVAFCLISAVLMVL LFIHIRRGLCWQRESYGNI in isoform 2.

VSP_036023

Natural variant

15

1

S → N: dbSNP rs17850347. Ref.7

VAR_047960

Natural variant

124

1

F → V: dbSNP rs17856254. Ref.7

VAR_047961

Natural variant

226

1

W → R: dbSNP rs17856253. Ref.7

VAR_047962

Natural variant

330

1

Y → H: dbSNP rs17851392. Ref.7

VAR_047963

Natural variant

360

1

V → A: dbSNP rs17850198. Ref.7

VAR_047964

Experimental info

Sequence conflict

15 – 24

10

SLGFLFLLFF → AFASCFCFFC in CAA37673. Ref.5

Sequence conflict

15 – 24

10

SLGFLFLLFF → ALASCFCFFC in AAA60022. Ref.3

Sequence conflict

15 – 24

10

SLGFLFLLFF → ALASCFCFFC in CAA36422. Ref.4

Sequence conflict

46

1

D → H in CAA37673. Ref.5

Sequence conflict

66 – 73

8

GFGQLTQL → RIWPTHPA in CAA37673. Ref.5

Sequence conflict

66 – 73

8

GFGQLTQL → WIWPTHPA in CAA36422. Ref.4

Sequence conflict

95

1

E → D in AAA60022. Ref.3

Sequence conflict

116

1

A → R in AAA60022. Ref.3

Sequence conflict

139

1

Q → E in CAA37673. Ref.5

Sequence conflict

157

1

P → R in CAA37673. Ref.5

Sequence conflict

212

1

P → A in CAA36422. Ref.4

Sequence conflict

215

1

C → S in AAA60022. Ref.3

Sequence conflict

294

1

S → T in AAA60022. Ref.3

Sequence conflict

372

1

C → V in AAA60022. Ref.3

Sequence conflict

383

1

D → N in CAA37673. Ref.5

Secondary structure

1

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386

Helix Strand Turn

Details...

Beta strand

34 – 43

10

Helix

60 – 62

3

Helix

72 – 88

17

Turn

89 – 93

5

Helix

99 – 101

3

Beta strand

102 – 108

7

Helix

110 – 123

14

Helix

128 – 130

3

Beta strand

144 – 146

3

Helix

148 – 150

3

Beta strand

152 – 155

4

Helix

162 – 173

12

Helix

175 – 181

7

Helix

182 – 184

3

Helix

185 – 195

11

Helix

202 – 208

7

Helix

210 – 218

9

Helix

229 – 247

19

Beta strand

248 – 251

4

Helix

252 – 258

7

Helix

261 – 276

16

Beta strand

282 – 288

7

Helix

290 – 299

10

Beta strand

313 – 321

9

Beta strand

324 – 332

9

Beta strand

349 – 352

4

Helix

353 – 360

8

Helix

361 – 363

3

Helix

368 – 371

4

Sequences

	Sequence		Length	Mass (Da)
	Isoform 1 [UniParc]. Last modified March 1, 1992. Version 3. Checksum: EF81E11DFAECADEA	FASTA	386	44,566
	10 20 30 40 50 60 MRAAPLLLAR AASLSLGFLF LLFFWLDRSV LAKELKFVTL VFRHGDRSPI DTFPTDPIKE 70 80 90 100 110 120 SSWPQGFGQL TQLGMEQHYE LGEYIRKRYR KFLNESYKHE QVYIRSTDVD RTLMSAMTNL 130 140 150 160 170 180 AALFPPEGVS IWNPILLWQP IPVHTVPLSE DQLLYLPFRN CPRFQELESE TLKSEEFQKR 190 200 210 220 230 240 LHPYKDFIAT LGKLSGLHGQ DLFGIWSKVY DPLYCESVHN FTLPSWATED TMTKLRELSE 250 260 270 280 290 300 LSLLSLYGIH KQKEKSRLQG GVLVNEILNH MKRATQIPSY KKLIMYSAHD TTVSGLQMAL 310 320 330 340 350 360 DVYNGLLPPY ASCHLTELYF EKGEYFVEMY YRNETQHEPY PLMLPGCSPS CPLERFAELV 370 380 GPVIPQDWST ECMTTNSHQG TEDSTD
	Isoform 2. Checksum: 68E1131197595362	FASTA	418	48,336
	10 20 30 40 50 60 MRAAPLLLAR AASLSLGFLF LLFFWLDRSV LAKELKFVTL VFRHGDRSPI DTFPTDPIKE 70 80 90 100 110 120 SSWPQGFGQL TQLGMEQHYE LGEYIRKRYR KFLNESYKHE QVYIRSTDVD RTLMSAMTNL 130 140 150 160 170 180 AALFPPEGVS IWNPILLWQP IPVHTVPLSE DQLLYLPFRN CPRFQELESE TLKSEEFQKR 190 200 210 220 230 240 LHPYKDFIAT LGKLSGLHGQ DLFGIWSKVY DPLYCESVHN FTLPSWATED TMTKLRELSE 250 260 270 280 290 300 LSLLSLYGIH KQKEKSRLQG GVLVNEILNH MKRATQIPSY KKLIMYSAHD TTVSGLQMAL 310 320 330 340 350 360 DVYNGLLPPY ASCHLTELYF EKGEYFVEMY YRNETQHEPY PLMLPGCSPS CPLERFAELV 370 380 390 400 410 GPVIPQDWST ECMTTNSHQV LKVIFAVAFC LISAVLMVLL FIHIRRGLCW QRESYGNI

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Structure of human prostatic acid phosphatase gene." Sharief F.S., Li S.S.-L. Biochem. Biophys. Res. Commun. 184:1468-1476(1992) [PubMed: 1375464] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] (ISOFORM 1).
[2]	"Covalent structure, disulfide bonding, and identification of reactive surface and active site residues of human prostatic acid phosphatase." van Etten R.L., Davidson R., Stevis P.E., Macarthur H., Moore D.L. J. Biol. Chem. 266:2313-2319(1991) [PubMed: 1989985] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 1), PARTIAL PROTEIN SEQUENCE, DISULFIDE BONDS, ACTIVE SITE.
[3]	"Human prostatic acid phosphatase: cDNA cloning, gene mapping and protein sequence homology with lysosomal acid phosphatase." Sharief F.S., Lee H., Leuderman M.M., Lundwall A., Deaven L.L., Lee C.-L., Li S.S.-L. Biochem. Biophys. Res. Commun. 160:79-86(1989) [PubMed: 2712834] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 1).
[4]	"Molecular cloning and sequence analysis of cDNA encoding human prostatic acid phosphatase." Vihko P., Virkkunen P., Henttu P., Roiko K., Solin T., Huhtala M.L. FEBS Lett. 236:275-281(1988) [PubMed: 2842184] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 1), PARTIAL PROTEIN SEQUENCE. Tissue: Prostate.
[5]	"Nucleotide sequence of human prostatic acid phosphatase determined from a full-length cDNA clone." Tailor P.G., Govindan M.V., Patel P.C. Nucleic Acids Res. 18:4928-4928(1990) [PubMed: 2395659] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 1). Tissue: Prostate.
[6]	"Nucleotide sequence of human prostatic acid phosphatase ACPP gene, including seven Alu repeats." Sharief F.S., Li S.S.-L. Biochem. Mol. Biol. Int. 33:561-565(1994) [PubMed: 7951074] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] (ISOFORM 1).
[7]	"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)." The MGC Project Team Genome Res. 14:2121-2127(2004) [PubMed: 15489334] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORMS 1 AND 2), VARIANTS ASN-15; VAL-124; ARG-226; HIS-330 AND ALA-360. Tissue: Prostate.
[8]	"Structural origins of L(+)-tartrate inhibition of human prostatic acid phosphatase." Lacount M.W., Handy G., Lebioda L. J. Biol. Chem. 273:30406-30409(1998) [PubMed: 9804805] [Abstract] Cited for: X-RAY CRYSTALLOGRAPHY (2.9 ANGSTROMS).
+	Additional computationally mapped references.

Cross-references

Sequence databases

EMBLGenBankDDBJ

M97589 , M97580, M97581, M97582, M97583, M97584, M97585, M97586, M97587, M97588 Genomic DNA. Translation: AAA60021.1.
M34840 mRNA. Translation: AAA69694.1.
M24902 mRNA. Translation: AAA60022.1.
X52174 mRNA. Translation: CAA36422.1.
X53605 mRNA. Translation: CAA37673.1.
U07097 , U07083, U07085, U07086, U07088, U07091, U07092, U07093, U07095 Genomic DNA. Translation: AAB60640.1.
BC007460 mRNA. Translation: AAH07460.1.
BC008493 mRNA. Translation: AAH08493.1.
BC016344 mRNA. Translation: AAH16344.1.

IPI

IPI00289983.
IPI00396434.

PIR

JH0610.

RefSeq

NP_001090.2.
NP_001127666.1.

UniGene

Hs.433060

3D structure databases

PDBPDBe

Entry	Method	Resolution (Å)	Chain	Positions	PDBsum
1CVI	X-ray	3.20	A/B/C/D	33-374	[»]
1ND5	X-ray	2.90	A/B/C/D	33-386	[»]
1ND6	X-ray	2.40	A/B/C/D	33-386	[»]
2HPA	X-ray	2.90	A/B/C/D	33-374	[»]

ModBase

Search...

Protein-protein interaction databases

STRING

P15309.

Genome annotation databases

Ensembl

ENST00000336375; ENSP00000337471; ENSG00000014257; Homo sapiens. [Genome view]
ENST00000351273; ENSP00000323036; ENSG00000014257; Homo sapiens. [Genome view]

GeneID

55.

KEGG

hsa:55.

UCSC

uc003eop.2. human.
uc010htp.1. human.

Organism-specific databases

CTD

55.

GeneCards

GC03P133518.

H-InvDB

HIX0022603.

HGNC

HGNC:125. ACPP.

HPA

CAB000071.
HPA004335.

MIM

171790. gene.

PharmGKB

PA24449.

GenAtlas

Search...

Phylogenomic databases

HOVERGEN

P15309.

OMA

VHNFTLP.

Enzyme and pathway databases

BRENDA

3.1.3.2. 247.

Gene expression databases

ArrayExpress

P15309.

Bgee

P15309.

CleanEx

HS_ACPP.

Genevestigator

P15309.

GermOnline

ENSG00000014257. Homo sapiens.

Family and domain databases

InterPro

IPR000560. Histidine_acid_Pase.
[Graphical view]

Pfam

PF00328. Acid_phosphat_A. 1 hit.
[Graphical view]

PROSITE

PS00616. HIS_ACID_PHOSPHAT_1. 1 hit.
PS00778. HIS_ACID_PHOSPHAT_2. 1 hit.
[Graphical view]

ProtoNet

Search...

Other Resources

NextBio

221.

PMAP-CutDB

P15309.

SOURCE

Search...

Entry information

Entry name

PPAP_HUMAN

Accession

Primary (citable) accession number: P15309
Secondary accession number(s): Q96KY0, Q96QK9, Q96QM0

Entry history

Integrated into UniProtKB/Swiss-Prot:	April 1, 1990
Last sequence update:	March 1, 1992
Last modified:	November 3, 2009
This is version 97 of the entry and version 3 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HPI (Human Proteome Initiative)

Disclaimer

Any medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.