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Reviewed, UniProtKB/Swiss-Prot P15309 (PPAP_HUMAN)

Last modified November 3, 2009. Version 97. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (6) | Third-party data | Customize display text xml rdf/xml gff fasta
Names and origin · Protein attributes · General annotation (Comments) · Ontologies · Alternative products · Sequence annotation (Features) · Sequences · References · Cross-references · Entry information · Relevant documents

Names and origin

Protein namesRecommended name:
    Prostatic acid phosphatase
    EC=3.1.3.2
Gene names
Name: ACPP
OrganismHomo sapiens (Human) [Complete proteome]
Taxonomic identifier9606 [NCBI]
Taxonomic lineageEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo

Protein attributes

Sequence length386 AA.
Sequence statusComplete.
Sequence processingThe displayed sequence is further processed into a mature form.
Protein existenceEvidence at protein level.

General annotation (Comments)

Catalytic activity

A phosphate monoester + H2O = an alcohol + phosphate.

Subunit structure

Homodimer.

Subcellular location

Isoform 1: Secreted By similarity.

Isoform 2: Lysosome membrane; Single-pass type I membrane protein By similarity. Note: Predominantly localized in the plasma membrane but also detected in intracellular vesicles By similarity.

Sequence similarities

Belongs to the histidine acid phosphatase family.

Ontologies

Keywords
   Cellular componentLysosome
Membrane
Secreted
   Coding sequence diversityAlternative splicing
Polymorphism
   DomainSignal
   Molecular functionHydrolase
   PTMDisulfide bond
Glycoprotein
   Technical term3D-structure
Complete proteome
Direct protein sequencing
Gene Ontology (GO)
   Cellular componentextracellular region

Inferred from electronic annotation. Source: UniProtKB-SubCell

integral to membrane

Inferred from electronic annotation. Source: UniProtKB-SubCell

lysosome

Inferred from electronic annotation. Source: UniProtKB-KW

   Molecular functionacid phosphatase activity

Inferred from electronic annotation. Source: EC

Complete GO annotation...

Alternative products

This entry describes 2 isoforms produced by alternative splicing. [Align] [Select]
Isoform 1 (identifier: P15309-1)

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.
Isoform 2 (identifier: P15309-2)

The sequence of this isoform differs from the canonical sequence as follows:
     380-386: GTEDSTD → VLKVIFAVAFCLISAVLMVLLFIHIRRGLCWQRESYGNI
Note: Predominantly localized in the plasma membrane but also detected in intracellular vesicles (By similarity).

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Signal peptide1 – 3232
Chain33 – 386354Prostatic acid phosphatase
PRO_0000023963

Sites

Active site441Nucleophile By similarity
Active site2901Proton donor Ref.2

Amino acid modifications

Glycosylation941N-linked (GlcNAc...)
Glycosylation2201N-linked (GlcNAc...)
Glycosylation3331N-linked (GlcNAc...)
Disulfide bond161 ↔ 372 Ref.2
Disulfide bond215 ↔ 313 Ref.2
Disulfide bond347 ↔ 351 Ref.2

Natural variations

Alternative sequence380 – 3867GTEDSTD → VLKVIFAVAFCLISAVLMVL LFIHIRRGLCWQRESYGNI in isoform 2.
VSP_036023
Natural variant151S → N: dbSNP rs17850347. Ref.7
VAR_047960
Natural variant1241F → V: dbSNP rs17856254. Ref.7
VAR_047961
Natural variant2261W → R: dbSNP rs17856253. Ref.7
VAR_047962
Natural variant3301Y → H: dbSNP rs17851392. Ref.7
VAR_047963
Natural variant3601V → A: dbSNP rs17850198. Ref.7
VAR_047964

Experimental info

Sequence conflict15 – 2410SLGFLFLLFF → AFASCFCFFC in CAA37673. Ref.5
Sequence conflict15 – 2410SLGFLFLLFF → ALASCFCFFC in AAA60022. Ref.3
Sequence conflict15 – 2410SLGFLFLLFF → ALASCFCFFC in CAA36422. Ref.4
Sequence conflict461D → H in CAA37673. Ref.5
Sequence conflict66 – 738GFGQLTQL → RIWPTHPA in CAA37673. Ref.5
Sequence conflict66 – 738GFGQLTQL → WIWPTHPA in CAA36422. Ref.4
Sequence conflict951E → D in AAA60022. Ref.3
Sequence conflict1161A → R in AAA60022. Ref.3
Sequence conflict1391Q → E in CAA37673. Ref.5
Sequence conflict1571P → R in CAA37673. Ref.5
Sequence conflict2121P → A in CAA36422. Ref.4
Sequence conflict2151C → S in AAA60022. Ref.3
Sequence conflict2941S → T in AAA60022. Ref.3
Sequence conflict3721C → V in AAA60022. Ref.3
Sequence conflict3831D → N in CAA37673. Ref.5

Secondary structure

................................................... 386
Helix Strand Turn

Details...

Sequences

Sequence LengthMass (Da)Tools
Isoform 1 [UniParc].

Last modified March 1, 1992. Version 3.
Checksum: EF81E11DFAECADEA

FASTA38644,566
        10         20         30         40         50         60 
MRAAPLLLAR AASLSLGFLF LLFFWLDRSV LAKELKFVTL VFRHGDRSPI DTFPTDPIKE 

        70         80         90        100        110        120 
SSWPQGFGQL TQLGMEQHYE LGEYIRKRYR KFLNESYKHE QVYIRSTDVD RTLMSAMTNL 

       130        140        150        160        170        180 
AALFPPEGVS IWNPILLWQP IPVHTVPLSE DQLLYLPFRN CPRFQELESE TLKSEEFQKR 

       190        200        210        220        230        240 
LHPYKDFIAT LGKLSGLHGQ DLFGIWSKVY DPLYCESVHN FTLPSWATED TMTKLRELSE 

       250        260        270        280        290        300 
LSLLSLYGIH KQKEKSRLQG GVLVNEILNH MKRATQIPSY KKLIMYSAHD TTVSGLQMAL 

       310        320        330        340        350        360 
DVYNGLLPPY ASCHLTELYF EKGEYFVEMY YRNETQHEPY PLMLPGCSPS CPLERFAELV 

       370        380 
GPVIPQDWST ECMTTNSHQG TEDSTD 

« Hide

Isoform 2.

Checksum: 68E1131197595362
Show »

FASTA41848,336

References

« Hide 'large scale' references
[1]"Structure of human prostatic acid phosphatase gene."
Sharief F.S., Li S.S.-L.
Biochem. Biophys. Res. Commun. 184:1468-1476(1992) [PubMed: 1375464] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] (ISOFORM 1).
[2]"Covalent structure, disulfide bonding, and identification of reactive surface and active site residues of human prostatic acid phosphatase."
van Etten R.L., Davidson R., Stevis P.E., Macarthur H., Moore D.L.
J. Biol. Chem. 266:2313-2319(1991) [PubMed: 1989985] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 1), PARTIAL PROTEIN SEQUENCE, DISULFIDE BONDS, ACTIVE SITE.
[3]"Human prostatic acid phosphatase: cDNA cloning, gene mapping and protein sequence homology with lysosomal acid phosphatase."
Sharief F.S., Lee H., Leuderman M.M., Lundwall A., Deaven L.L., Lee C.-L., Li S.S.-L.
Biochem. Biophys. Res. Commun. 160:79-86(1989) [PubMed: 2712834] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 1).
[4]"Molecular cloning and sequence analysis of cDNA encoding human prostatic acid phosphatase."
Vihko P., Virkkunen P., Henttu P., Roiko K., Solin T., Huhtala M.L.
FEBS Lett. 236:275-281(1988) [PubMed: 2842184] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 1), PARTIAL PROTEIN SEQUENCE.
Tissue: Prostate.
[5]"Nucleotide sequence of human prostatic acid phosphatase determined from a full-length cDNA clone."
Tailor P.G., Govindan M.V., Patel P.C.
Nucleic Acids Res. 18:4928-4928(1990) [PubMed: 2395659] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 1).
Tissue: Prostate.
[6]"Nucleotide sequence of human prostatic acid phosphatase ACPP gene, including seven Alu repeats."
Sharief F.S., Li S.S.-L.
Biochem. Mol. Biol. Int. 33:561-565(1994) [PubMed: 7951074] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] (ISOFORM 1).
[7]"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
The MGC Project Team
Genome Res. 14:2121-2127(2004) [PubMed: 15489334] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORMS 1 AND 2), VARIANTS ASN-15; VAL-124; ARG-226; HIS-330 AND ALA-360.
Tissue: Prostate.
[8]"Structural origins of L(+)-tartrate inhibition of human prostatic acid phosphatase."
Lacount M.W., Handy G., Lebioda L.
J. Biol. Chem. 273:30406-30409(1998) [PubMed: 9804805] [Abstract]
Cited for: X-RAY CRYSTALLOGRAPHY (2.9 ANGSTROMS).
+Additional computationally mapped references.

Cross-references

Sequence databases

M97589 expand/collapse EMBL AC list , M97580, M97581, M97582, M97583, M97584, M97585, M97586, M97587, M97588 Genomic DNA. Translation: AAA60021.1.
M34840 mRNA. Translation: AAA69694.1.
M24902 mRNA. Translation: AAA60022.1.
X52174 mRNA. Translation: CAA36422.1.
X53605 mRNA. Translation: CAA37673.1.
U07097 expand/collapse EMBL AC list , U07083, U07085, U07086, U07088, U07091, U07092, U07093, U07095 Genomic DNA. Translation: AAB60640.1.
BC007460 mRNA. Translation: AAH07460.1.
BC008493 mRNA. Translation: AAH08493.1.
BC016344 mRNA. Translation: AAH16344.1.
IPIIPI00289983.
IPI00396434.
PIRJH0610.
RefSeqNP_001090.2.
NP_001127666.1.
UniGeneHs.433060

3D structure databases

EntryMethodResolution (Å)ChainPositionsPDBsum
1CVIX-ray3.20A/B/C/D33-374[»]
1ND5X-ray2.90A/B/C/D33-386[»]
1ND6X-ray2.40A/B/C/D33-386[»]
2HPAX-ray2.90A/B/C/D33-374[»]
ModBaseSearch...

Protein-protein interaction databases

STRINGP15309.

Genome annotation databases

EnsemblENST00000336375; ENSP00000337471; ENSG00000014257; Homo sapiens. [Genome view]
ENST00000351273; ENSP00000323036; ENSG00000014257; Homo sapiens. [Genome view]
GeneID55.
KEGGhsa:55.
UCSCuc003eop.2. human.
uc010htp.1. human.

Organism-specific databases

CTD55.
GeneCardsGC03P133518.
H-InvDBHIX0022603.
HGNCHGNC:125. ACPP.
HPACAB000071.
HPA004335.
MIM171790. gene.
PharmGKBPA24449.
GenAtlasSearch...

Phylogenomic databases

HOVERGENP15309.
OMAVHNFTLP.

Enzyme and pathway databases

BRENDA3.1.3.2. 247.

Gene expression databases

ArrayExpressP15309.
BgeeP15309.
CleanExHS_ACPP.
GenevestigatorP15309.
GermOnlineENSG00000014257. Homo sapiens.

Family and domain databases

InterProIPR000560. Histidine_acid_Pase.
[Graphical view]
PfamPF00328. Acid_phosphat_A. 1 hit.
[Graphical view]
PROSITEPS00616. HIS_ACID_PHOSPHAT_1. 1 hit.
PS00778. HIS_ACID_PHOSPHAT_2. 1 hit.
[Graphical view]
ProtoNetSearch...

Other Resources

NextBio221.
PMAP-CutDBP15309.
SOURCESearch...

Entry information

Entry namePPAP_HUMAN
AccessionPrimary (citable) accession number: P15309
Secondary accession number(s): Q96KY0, Q96QK9, Q96QM0
Entry history
Integrated into UniProtKB/Swiss-Prot: April 1, 1990
Last sequence update: March 1, 1992
Last modified: November 3, 2009
This is version 97 of the entry and version 3 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation projectHPI (Human Proteome Initiative)
DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

Relevant documents

Human chromosome 3

Human chromosome 3: entries, gene names and cross-references to MIM

Human entries with polymorphisms or disease mutations

List of human entries with polymorphisms or disease mutations

Human polymorphisms and disease mutations

Index of human polymorphisms and disease mutations

MIM cross-references

Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot

PDB cross-references

Index of Protein Data Bank (PDB) cross-references

SIMILARITY comments

Index of protein domains and families

Names and origin · Protein attributes · General annotation (Comments) · Ontologies · Alternative products · Sequence annotation (Features) · Sequences · References · Cross-references · Entry information · Relevant documents