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P15304

- LIPS_RAT

UniProt

P15304 - LIPS_RAT

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Protein

Hormone-sensitive lipase

Gene

Lipe

Organism
Rattus norvegicus (Rat)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at protein leveli

Functioni

In adipose tissue and heart, it primarily hydrolyzes stored triglycerides to free fatty acids, while in steroidogenic tissues, it principally converts cholesteryl esters to free cholesterol for steroid hormone production.

Catalytic activityi

Diacylglycerol + H2O = monoacylglycerol + a carboxylate.
Triacylglycerol + H2O = diacylglycerol + a carboxylate.
Monoacylglycerol + H2O = glycerol + a carboxylate.

Enzyme regulationi

Rapidly activated by cAMP-dependent phosphorylation under the influence of catecholamines. Dephosphorylation and inactivation are controlled by insulin.

Pathwayi

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Active sitei649 – 6491PROSITE-ProRule annotation
Active sitei723 – 7231PROSITE-ProRule annotation

GO - Molecular functioni

  1. acylglycerol lipase activity Source: RGD
  2. hormone-sensitive lipase activity Source: UniProtKB-EC
  3. hydrolase activity, acting on ester bonds Source: RGD
  4. rRNA primary transcript binding Source: UniProtKB

GO - Biological processi

  1. cholesterol metabolic process Source: UniProtKB-KW
  2. female pregnancy Source: RGD
  3. lipid catabolic process Source: UniProtKB
  4. response to drug Source: RGD
  5. termination of RNA polymerase I transcription Source: UniProtKB
  6. transcription initiation from RNA polymerase I promoter Source: UniProtKB
  7. triglyceride catabolic process Source: RGD
Complete GO annotation...

Keywords - Molecular functioni

Hydrolase

Keywords - Biological processi

Cholesterol metabolism, Lipid degradation, Lipid metabolism, Steroid metabolism, Sterol metabolism

Enzyme and pathway databases

UniPathwayiUPA00256.

Protein family/group databases

MEROPSiS09.993.

Names & Taxonomyi

Protein namesi
Recommended name:
Hormone-sensitive lipase (EC:3.1.1.79)
Short name:
HSL
Gene namesi
Name:Lipe
OrganismiRattus norvegicus (Rat)
Taxonomic identifieri10116 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeRattus
ProteomesiUP000002494: Unplaced

Organism-specific databases

RGDi3010. Lipe.

Subcellular locationi

Cell membrane By similarity. Membranecaveola By similarity. Cytoplasmcytosol By similarity
Note: Found in the high-density caveolae. Translocates to the cytoplasm from the caveolae upon insulin stimulation.By similarity

GO - Cellular componenti

  1. caveola Source: UniProtKB
  2. cytoplasm Source: UniProtKB
  3. cytosol Source: Reactome
  4. extracellular space Source: RGD
  5. lipid particle Source: UniProtKB
  6. mitochondrion Source: UniProtKB
  7. nucleus Source: UniProtKB
Complete GO annotation...

Keywords - Cellular componenti

Cell membrane, Cytoplasm, Membrane

Pathology & Biotechi

Mutagenesis

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Mutagenesisi863 – 8631S → A or E: No effect on activation by PKA. 1 Publication
Mutagenesisi865 – 8651S → A: Increases activation by PKA. 1 Publication
Mutagenesisi865 – 8651S → E: No effect on activation by PKA. 1 Publication
Mutagenesisi959 – 9591S → A: Slightly decreases activation by PKA. Abolishes activation by PKA; when associated with A-960. 1 Publication
Mutagenesisi960 – 9601S → A: No effect on activation by PKA. Abolishes activation by PKA; when associated with A-959. 1 Publication

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 10681068Hormone-sensitive lipasePRO_0000071550Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei863 – 8631Phosphoserine; by PKA1 Publication
Modified residuei865 – 8651Phosphoserine; by AMPKBy similarity
Modified residuei959 – 9591Phosphoserine; by PKA1 Publication
Modified residuei960 – 9601Phosphoserine; by PKA1 Publication

Post-translational modificationi

Phosphorylation by AMPK may block translocation to lipid droplets.By similarity

Keywords - PTMi

Phosphoprotein

Proteomic databases

PaxDbiP15304.
PRIDEiP15304.

PTM databases

PhosphoSiteiP15304.

Expressioni

Gene expression databases

GenevestigatoriP15304.

Interactioni

Subunit structurei

Interacts with PTRF in the adipocyte cytoplasm (By similarity). Interacts with PLIN5.By similarity1 Publication

Protein-protein interaction databases

BioGridi247369. 2 interactions.
IntActiP15304. 1 interaction.
MINTiMINT-4783535.

Structurei

3D structure databases

ProteinModelPortaliP15304.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Motif

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Motifi649 – 6513Involved in the stabilization of the negatively charged intermediate by the formation of the oxyanion holeBy similarity

Sequence similaritiesi

Belongs to the 'GDXG' lipolytic enzyme family.Curated

Phylogenomic databases

eggNOGiCOG0657.
HOGENOMiHOG000047722.
HOVERGENiHBG000187.
InParanoidiP15304.
KOiK07188.
PhylomeDBiP15304.

Family and domain databases

Gene3Di3.40.50.1820. 2 hits.
InterProiIPR029058. AB_hydrolase.
IPR013094. AB_hydrolase_3.
IPR010468. HSL_N.
IPR002168. Lipase_GDXG_AS.
[Graphical view]
PfamiPF07859. Abhydrolase_3. 2 hits.
PF06350. HSL_N. 1 hit.
[Graphical view]
SUPFAMiSSF53474. SSF53474. 2 hits.
PROSITEiPS01173. LIPASE_GDXG_HIS. 1 hit.
PS01174. LIPASE_GDXG_SER. 1 hit.
[Graphical view]

Sequences (2)i

Sequence statusi: Complete.

This entry describes 2 isoformsi produced by alternative splicing. Align

Isoform 1 (identifier: P15304-1) [UniParc]FASTAAdd to Basket

Also known as: Testicular

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MKPRRPISFT REITAMEPSS TSVSRPEWRP EAQQTLTDYP GSRELQEFGI
60 70 80 90 100
PQKQSLPNEA TAQQGAEFQQ EQGVQQSTLL QKLLTPLAFP VPQQSFPSHK
110 120 130 140 150
VHSDQQEATS QNGPGAGKVH TTQKELEHRD EHVGTAESGP AEPPPATEVE
160 170 180 190 200
ATSIAQAVSG PDKKLPTQTD LVSQERAEQS DPTAQQTPLV QGVKSDQGSL
210 220 230 240 250
IESGILARLQ KLAIQQPSQE WKTFLDCVTE SDMEKYLNSS SKSNPPEPSG
260 270 280 290 300
GTVIPGTLPS KQKPDCGKMS GYGGKLPHGK KGILQKHKHY WDTASAFSHS
310 320 330 340 350
MDLRTMTQSL VALAEDNMAF FSSQGPGETA RRLSNVFAGV REQALGLEPT
360 370 380 390 400
LGQLLGVAHH FDLDTETPAN GYRSLVHTAR CCLAHLLHKS RYVASNRRSI
410 420 430 440 450
FFRASHNLAE LEAYLAALTQ LRALAYYAQR LLTINRPGVL FFEGDEGLSA
460 470 480 490 500
DFLQDYVTLH KGCFYGRCLG FQFTPAIRPF LQTLSIGLVS FGEHYKRNET
510 520 530 540 550
GLSVTASSLF TGGRFAIDPE LRGAEFERII QNLDVHFWKA FWNITEIEVL
560 570 580 590 600
SSLANMASTT VRVSRLLSLP PEAFEMPLTS DPKLTVTISP PLAHTGPGPV
610 620 630 640 650
LARLISYDLR EGQDSKMLNS LAKSEGPRLE LRPRPQQAPR SRALVVHIHG
660 670 680 690 700
GGFVAQTSKS HEPYLKNWAQ ELGVPIISID YSLAPEAPFP RALEECFFAY
710 720 730 740 750
CWAVKHCELL GSTGERICLA GDSAGGNLCI TVSLRAAAYG VRVPDGIMAA
760 770 780 790 800
YPVTTLQSSA SPSRLLSLMD PLLPLSVLSK CVSAYSGTET EDHFDSDQKA
810 820 830 840 850
LGVMGLVQRD TSLFLRDLRL GASSWLNSFL ELSGRKPHKT PLPATETLRP
860 870 880 890 900
TDSGRLTESM RRSVSEAALA QPEGLLGTDS LKKLTIKDLS FKGNSEPSDS
910 920 930 940 950
PEMSQSMETL GPSTPSDVNF FLRSGNSQEE AETRDDISPM DGIPRVRAAF
960 970 980 990 1000
PDGFHPRRSS QGVLHMPLYS SPIVKNPFMS PLLAPDVMLK TLPPVHLVAC
1010 1020 1030 1040 1050
ALDPMLDDSV MFARRLKDLG QPVTLKVVED LPHGFLSLAA LCRETRQAAE
1060
LCVQRIRLIL TPPAAPLT
Length:1,068
Mass (Da):116,812
Last modified:January 24, 2006 - v3
Checksum:i13B10C9315AC87F1
GO
Isoform 2 (identifier: P15304-2) [UniParc]FASTAAdd to Basket

The sequence of this isoform differs from the canonical sequence as follows:
     1-300: Missing.

Show »
Length:768
Mass (Da):84,170
Checksum:i90A1F0432DAC8B4C
GO

Alternative sequence

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Alternative sequencei1 – 300300Missing in isoform 2. 2 PublicationsVSP_017117Add
BLAST

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
X51415 mRNA. Translation: CAA35777.1.
U40001 mRNA. Translation: AAC52771.1.
PIRiS03672. LIRTH.
RefSeqiNP_036991.1. NM_012859.1. [P15304-1]
XP_006228456.1. XM_006228394.2. [P15304-2]
UniGeneiRn.10566.

Genome annotation databases

GeneIDi25330.
KEGGirno:25330.
UCSCiRGD:3010. rat. [P15304-1]

Keywords - Coding sequence diversityi

Alternative splicing

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
X51415 mRNA. Translation: CAA35777.1 .
U40001 mRNA. Translation: AAC52771.1 .
PIRi S03672. LIRTH.
RefSeqi NP_036991.1. NM_012859.1. [P15304-1 ]
XP_006228456.1. XM_006228394.2. [P15304-2 ]
UniGenei Rn.10566.

3D structure databases

ProteinModelPortali P15304.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

BioGridi 247369. 2 interactions.
IntActi P15304. 1 interaction.
MINTi MINT-4783535.

Chemistry

BindingDBi P15304.
ChEMBLi CHEMBL5582.

Protein family/group databases

MEROPSi S09.993.

PTM databases

PhosphoSitei P15304.

Proteomic databases

PaxDbi P15304.
PRIDEi P15304.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

GeneIDi 25330.
KEGGi rno:25330.
UCSCi RGD:3010. rat. [P15304-1 ]

Organism-specific databases

CTDi 3991.
RGDi 3010. Lipe.

Phylogenomic databases

eggNOGi COG0657.
HOGENOMi HOG000047722.
HOVERGENi HBG000187.
InParanoidi P15304.
KOi K07188.
PhylomeDBi P15304.

Enzyme and pathway databases

UniPathwayi UPA00256 .

Miscellaneous databases

NextBioi 606207.
PROi P15304.

Gene expression databases

Genevestigatori P15304.

Family and domain databases

Gene3Di 3.40.50.1820. 2 hits.
InterProi IPR029058. AB_hydrolase.
IPR013094. AB_hydrolase_3.
IPR010468. HSL_N.
IPR002168. Lipase_GDXG_AS.
[Graphical view ]
Pfami PF07859. Abhydrolase_3. 2 hits.
PF06350. HSL_N. 1 hit.
[Graphical view ]
SUPFAMi SSF53474. SSF53474. 2 hits.
PROSITEi PS01173. LIPASE_GDXG_HIS. 1 hit.
PS01174. LIPASE_GDXG_SER. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

  1. "Nucleotide sequence of rat adipose hormone sensitive lipase cDNA."
    Holm C., Kirchgessner T.G., Svenson K.L., Lusis A.J., Belfrage P., Schotz M.C.
    Nucleic Acids Res. 16:9879-9879(1988) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 2).
    Tissue: Adipose tissue.
  2. Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 2).
    Tissue: Adipose tissue.
  3. Holm C.
    Submitted (JUL-1995) to the EMBL/GenBank/DDBJ databases
    Cited for: SEQUENCE REVISION TO 842-855 AND 1046-1068.
  4. "Molecular cloning, genomic organization, and expression of a testicular isoform of hormone-sensitive lipase."
    Holst L.S., Langin D., Mulder H., Laurell H., Grober J., Bergh A., Mohrenweiser H.W., Edgren G., Holm C.
    Genomics 35:441-447(1996) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 1).
    Strain: Sprague-Dawley.
    Tissue: Testis.
  5. "Identification of novel phosphorylation sites in hormone-sensitive lipase that are phosphorylated in response to isoproterenol and govern activation properties in vitro."
    Anthonsen M.W., Roennstrand L., Wernstedt C., Degerman E., Holm C.
    J. Biol. Chem. 273:215-221(1998) [PubMed] [Europe PMC] [Abstract]
    Cited for: PHOSPHORYLATION AT SER-863; SER-959 AND SER-960, MUTAGENESIS OF SER-863; SER-865; SER-959 AND SER-960.
  6. "Skeletal muscle PLIN3 and PLIN5 are serine phosphorylated at rest and following lipolysis during adrenergic or contractile stimulation."
    Macpherson R.E., Vandenboom R., Roy B.D., Peters S.J.
    Physiol. Rep. 1:E00084-E00084(2013) [PubMed] [Europe PMC] [Abstract]
    Cited for: INTERACTION WITH LIPE AND PNPLA2, PHOSPHORYLATION.

Entry informationi

Entry nameiLIPS_RAT
AccessioniPrimary (citable) accession number: P15304
Secondary accession number(s): Q6LCQ2
Entry historyi
Integrated into UniProtKB/Swiss-Prot: April 1, 1990
Last sequence update: January 24, 2006
Last modified: October 29, 2014
This is version 127 of the entry and version 3 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3