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Protein

Protein transport protein SEC23

Gene

SEC23

Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Component of the coat protein complex II (COPII) which promotes the formation of transport vesicles from the endoplasmic reticulum (ER). The coat has two main functions, the physical deformation of the endoplasmic reticulum membrane into vesicles and the selection of cargo molecules. SEC23 interacts with BET3 in order to target TRAPPI complex to COPII involved in internalisation of plasma membrane proteins like the maltose transporter.22 Publications

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Metal bindingi56 – 561Zinc1 Publication
Metal bindingi61 – 611Zinc1 Publication
Metal bindingi80 – 801Zinc1 Publication
Metal bindingi83 – 831Zinc1 Publication

GO - Molecular functioni

  • GTPase activator activity Source: FlyBase
  • zinc ion binding Source: InterPro

GO - Biological processi

  • ER to Golgi vesicle-mediated transport Source: InterPro
  • intracellular protein transport Source: InterPro
  • macroautophagy Source: SGD
  • positive regulation of GTPase activity Source: FlyBase
  • regulation of COPII vesicle coating Source: SGD
Complete GO annotation...

Keywords - Biological processi

ER-Golgi transport, Protein transport, Transport

Keywords - Ligandi

Metal-binding, Zinc

Enzyme and pathway databases

BioCyciYEAST:G3O-34306-MONOMER.
ReactomeiR-SCE-204005. COPII (Coat Protein 2) Mediated Vesicle Transport.
R-SCE-5694530. Cargo concentration in the ER.
R-SCE-983170. Antigen Presentation: Folding, assembly and peptide loading of class I MHC.

Names & Taxonomyi

Protein namesi
Recommended name:
Protein transport protein SEC23
Gene namesi
Name:SEC23
Ordered Locus Names:YPR181C
ORF Names:P9705.14
OrganismiSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Taxonomic identifieri559292 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces
Proteomesi
  • UP000002311 Componenti: Chromosome XVI

Organism-specific databases

EuPathDBiFungiDB:YPR181C.
SGDiS000006385. SEC23.

Subcellular locationi

GO - Cellular componenti

  • COPII vesicle coat Source: SGD
  • endoplasmic reticulum membrane Source: UniProtKB-SubCell
  • Golgi membrane Source: UniProtKB-SubCell
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm, Cytoplasmic vesicle, Endoplasmic reticulum, Golgi apparatus, Membrane

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 768768Protein transport protein SEC23PRO_0000205145Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei1 – 11N-acetylmethionineCombined sources

Post-translational modificationi

Ubiquitinated. Ubiquitination is required for the formation of the SEC23/24 complex. Deubiquitinated by the UBP3/BRE5 complex.1 Publication

Keywords - PTMi

Acetylation, Ubl conjugation

Proteomic databases

MaxQBiP15303.

PTM databases

iPTMnetiP15303.

Interactioni

Subunit structurei

The COPII coat is composed of at least 7 proteins: the SEC23/24 complex, the SEC13/31 complex, SFB2, SFB3 and the protein SAR1. Forms two other heterodimeric complexes with SFB3 and PDR17. Interacts with BET1, BET3, BOS1, EMP24, GRH1, SEC16, SEC22 and SYS1.18 Publications

Binary interactionsi

WithEntry#Exp.IntActNotes
SEC16P484155EBI-16584,EBI-16551
SEC24P404824EBI-16584,EBI-16592
SEC31P389684EBI-16584,EBI-20524
SFB2P539535EBI-16584,EBI-17006

Protein-protein interaction databases

BioGridi36353. 131 interactions.
DIPiDIP-2232N.
IntActiP15303. 36 interactions.
MINTiMINT-540233.

Structurei

Secondary structure

1
768
Legend: HelixTurnBeta strand
Show more details
Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Helixi3 – 108Combined sources
Beta strandi11 – 2212Combined sources
Helixi23 – 286Combined sources
Beta strandi33 – 375Combined sources
Beta strandi48 – 514Combined sources
Turni59 – 613Combined sources
Beta strandi69 – 724Combined sources
Turni73 – 764Combined sources
Beta strandi77 – 793Combined sources
Turni81 – 833Combined sources
Beta strandi86 – 883Combined sources
Helixi91 – 933Combined sources
Beta strandi98 – 1003Combined sources
Helixi103 – 1053Combined sources
Beta strandi108 – 1136Combined sources
Beta strandi123 – 1297Combined sources
Helixi134 – 14916Combined sources
Beta strandi156 – 16813Combined sources
Beta strandi172 – 18312Combined sources
Helixi190 – 1989Combined sources
Beta strandi221 – 2233Combined sources
Helixi224 – 2274Combined sources
Beta strandi228 – 2303Combined sources
Helixi231 – 24313Combined sources
Helixi262 – 27615Combined sources
Beta strandi283 – 2908Combined sources
Beta strandi294 – 2974Combined sources
Helixi311 – 3155Combined sources
Helixi322 – 33918Combined sources
Beta strandi342 – 3487Combined sources
Helixi355 – 36511Combined sources
Beta strandi369 – 3735Combined sources
Helixi378 – 3869Combined sources
Beta strandi394 – 3974Combined sources
Beta strandi399 – 40810Combined sources
Beta strandi412 – 4209Combined sources
Beta strandi437 – 4393Combined sources
Beta strandi443 – 4508Combined sources
Beta strandi456 – 4627Combined sources
Beta strandi484 – 49512Combined sources
Turni496 – 4983Combined sources
Beta strandi499 – 51214Combined sources
Helixi517 – 5215Combined sources
Helixi525 – 53915Combined sources
Helixi545 – 56319Combined sources
Beta strandi564 – 5674Combined sources
Helixi571 – 5733Combined sources
Turni578 – 5814Combined sources
Helixi582 – 59211Combined sources
Turni594 – 5963Combined sources
Helixi603 – 61311Combined sources
Helixi618 – 6258Combined sources
Beta strandi628 – 6325Combined sources
Beta strandi634 – 6363Combined sources
Helixi645 – 6473Combined sources
Beta strandi653 – 6575Combined sources
Beta strandi659 – 6668Combined sources
Helixi668 – 6769Combined sources
Helixi678 – 6803Combined sources
Helixi682 – 6843Combined sources
Helixi685 – 70319Combined sources
Beta strandi710 – 7156Combined sources
Helixi719 – 7213Combined sources
Helixi722 – 7254Combined sources
Beta strandi740 – 7423Combined sources
Helixi752 – 76312Combined sources

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
1M2OX-ray2.50A/C1-768[»]
1M2VX-ray2.75A1-768[»]
2QTVX-ray2.50A2-768[»]
4BZIelectron microscopy23.00A/D/G1-768[»]
ProteinModelPortaliP15303.
SMRiP15303. Positions 2-768.
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiP15303.

Family & Domainsi

Sequence similaritiesi

Belongs to the SEC23/SEC24 family. SEC23 subfamily.Curated

Phylogenomic databases

GeneTreeiENSGT00390000006916.
HOGENOMiHOG000231690.
InParanoidiP15303.
KOiK14006.
OMAiEYITARP.
OrthoDBiEOG092C0WSG.

Family and domain databases

Gene3Di3.40.20.10. 1 hit.
3.40.50.410. 1 hit.
InterProiIPR029006. ADF-H/Gelsolin-like_dom.
IPR007123. Gelsolin-like_dom.
IPR006900. Sec23/24_helical_dom.
IPR006896. Sec23/24_trunk_dom.
IPR012990. Sec23_24_beta_S.
IPR002035. VWF_A.
IPR006895. Znf_Sec23_Sec24.
[Graphical view]
PfamiPF00626. Gelsolin. 1 hit.
PF08033. Sec23_BS. 1 hit.
PF04815. Sec23_helical. 1 hit.
PF04811. Sec23_trunk. 1 hit.
PF04810. zf-Sec23_Sec24. 1 hit.
[Graphical view]
SUPFAMiSSF53300. SSF53300. 1 hit.
SSF81811. SSF81811. 1 hit.
SSF82754. SSF82754. 1 hit.
SSF82919. SSF82919. 1 hit.

Sequencei

Sequence statusi: Complete.

P15303-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MDFETNEDIN GVRFTWNVFP STRSDANSNV VPVGCLYTPL KEYDELNVAP
60 70 80 90 100
YNPVVCSGPH CKSILNPYCV IDPRNSSWSC PICNSRNHLP PQYTNLSQEN
110 120 130 140 150
MPLELQSTTI EYITNKPVTV PPIFFFVVDL TSETENLDSL KESIITSLSL
160 170 180 190 200
LPPNALIGLI TYGNVVQLHD LSSETIDRCN VFRGDREYQL EALTEMLTGQ
210 220 230 240 250
KPTGPGGAAS HLPNAMNKVT PFSLNRFFLP LEQVEFKLNQ LLENLSPDQW
260 270 280 290 300
SVPAGHRPLR ATGSALNIAS LLLQGCYKNI PARIILFASG PGTVAPGLIV
310 320 330 340 350
NSELKDPLRS HHDIDSDHAQ HYKKACKFYN QIAQRVAANG HTVDIFAGCY
360 370 380 390 400
DQIGMSEMKQ LTDSTGGVLL LTDAFSTAIF KQSYLRLFAK DEEGYLKMAF
410 420 430 440 450
NGNMAVKTSK DLKVQGLIGH ASAVKKTDAN NISESEIGIG ATSTWKMASL
460 470 480 490 500
SPYHSYAIFF EIANTAANSN PMMSAPGSAD RPHLAYTQFI TTYQHSSGTN
510 520 530 540 550
RIRVTTVANQ LLPFGTPAIA ASFDQEAAAV LMARIAVHKA ETDDGADVIR
560 570 580 590 600
WLDRTLIKLC QKYADYNKDD PQSFRLAPNF SLYPQFTYYL RRSQFLSVFN
610 620 630 640 650
NSPDETAFYR HIFTREDTTN SLIMIQPTLT SFSMEDDPQP VLLDSISVKP
660 670 680 690 700
NTILLLDTFF FILIYHGEQI AQWRKAGYQD DPQYADFKAL LEEPKLEAAE
710 720 730 740 750
LLVDRFPLPR FIDTEAGGSQ ARFLLSKLNP SDNYQDMARG GSTIVLTDDV
760
SLQNFMTHLQ QVAVSGQA
Length:768
Mass (Da):85,385
Last modified:April 1, 1990 - v1
Checksum:i69811913848265FB
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X15474 Genomic DNA. Translation: CAA33501.1.
U25842 Genomic DNA. Translation: AAB68114.1.
BK006949 Genomic DNA. Translation: DAA11597.1.
PIRiS05742. BVBY23.
RefSeqiNP_015507.1. NM_001184278.1.

Genome annotation databases

EnsemblFungiiYPR181C; YPR181C; YPR181C.
GeneIDi856311.
KEGGisce:YPR181C.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X15474 Genomic DNA. Translation: CAA33501.1.
U25842 Genomic DNA. Translation: AAB68114.1.
BK006949 Genomic DNA. Translation: DAA11597.1.
PIRiS05742. BVBY23.
RefSeqiNP_015507.1. NM_001184278.1.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
1M2OX-ray2.50A/C1-768[»]
1M2VX-ray2.75A1-768[»]
2QTVX-ray2.50A2-768[»]
4BZIelectron microscopy23.00A/D/G1-768[»]
ProteinModelPortaliP15303.
SMRiP15303. Positions 2-768.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi36353. 131 interactions.
DIPiDIP-2232N.
IntActiP15303. 36 interactions.
MINTiMINT-540233.

PTM databases

iPTMnetiP15303.

Proteomic databases

MaxQBiP15303.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblFungiiYPR181C; YPR181C; YPR181C.
GeneIDi856311.
KEGGisce:YPR181C.

Organism-specific databases

EuPathDBiFungiDB:YPR181C.
SGDiS000006385. SEC23.

Phylogenomic databases

GeneTreeiENSGT00390000006916.
HOGENOMiHOG000231690.
InParanoidiP15303.
KOiK14006.
OMAiEYITARP.
OrthoDBiEOG092C0WSG.

Enzyme and pathway databases

BioCyciYEAST:G3O-34306-MONOMER.
ReactomeiR-SCE-204005. COPII (Coat Protein 2) Mediated Vesicle Transport.
R-SCE-5694530. Cargo concentration in the ER.
R-SCE-983170. Antigen Presentation: Folding, assembly and peptide loading of class I MHC.

Miscellaneous databases

EvolutionaryTraceiP15303.
PROiP15303.

Family and domain databases

Gene3Di3.40.20.10. 1 hit.
3.40.50.410. 1 hit.
InterProiIPR029006. ADF-H/Gelsolin-like_dom.
IPR007123. Gelsolin-like_dom.
IPR006900. Sec23/24_helical_dom.
IPR006896. Sec23/24_trunk_dom.
IPR012990. Sec23_24_beta_S.
IPR002035. VWF_A.
IPR006895. Znf_Sec23_Sec24.
[Graphical view]
PfamiPF00626. Gelsolin. 1 hit.
PF08033. Sec23_BS. 1 hit.
PF04815. Sec23_helical. 1 hit.
PF04811. Sec23_trunk. 1 hit.
PF04810. zf-Sec23_Sec24. 1 hit.
[Graphical view]
SUPFAMiSSF53300. SSF53300. 1 hit.
SSF81811. SSF81811. 1 hit.
SSF82754. SSF82754. 1 hit.
SSF82919. SSF82919. 1 hit.
ProtoNetiSearch...

Entry informationi

Entry nameiSEC23_YEAST
AccessioniPrimary (citable) accession number: P15303
Secondary accession number(s): D6W4I1
Entry historyi
Integrated into UniProtKB/Swiss-Prot: April 1, 1990
Last sequence update: April 1, 1990
Last modified: September 7, 2016
This is version 164 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

Documents

  1. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  2. SIMILARITY comments
    Index of protein domains and families
  3. Yeast
    Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD
  4. Yeast chromosome XVI
    Yeast (Saccharomyces cerevisiae) chromosome XVI: entries and gene names

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.