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P15292 (P3P_LACLS) Reviewed, UniProtKB/Swiss-Prot

Last modified May 1, 2013. Version 107. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
PIII-type proteinase
EC=3.4.21.96
Alternative name(s):
Cell wall-associated serine proteinase
Lactocepin
Gene names
Name:prtP
Ordered Locus Names:LACR_C42
Encoded onPlasmid pSK111 Ref.1
Plasmid pLACR3 Ref.2
OrganismLactococcus lactis subsp. cremoris (strain SK11) [Complete proteome] [HAMAP]
Taxonomic identifier272622 [NCBI]
Taxonomic lineageBacteria › Firmicutes › Bacilli › Lactobacillales › Streptococcaceae › Lactococcus ›

Protein attributes

Sequence length1962 AA.
Sequence statusComplete.
Sequence processingThe displayed sequence is further processed into a mature form.
Protein existenceEvidence at protein level

General annotation (Comments)

Function

Protease which breaks down milk proteins during the growth of the bacteria on milk.

Catalytic activity

Endopeptidase activity with very broad specificity, although some subsite preference have been noted, e.g. large hydrophobic residues in the P1 and P4 positions, and Pro in the P2 position. Best known for its action on caseins, although it has been shown to hydrolyze hemoglobin and oxidized insulin B-chain.

Subcellular location

Secreted › cell wall; Peptidoglycan-anchor Potential.

Sequence similarities

Belongs to the peptidase S8 family.

Contains 2 FIVAR domains.

Ontologies

Keywords
   Cellular componentCell wall
Secreted
   DomainRepeat
Signal
   Molecular functionHydrolase
Protease
Serine protease
   PTMPeptidoglycan-anchor
Zymogen
   Technical termComplete proteome
Direct protein sequencing
Plasmid
Gene Ontology (GO)
   Biological_processproteolysis

Inferred from electronic annotation. Source: UniProtKB-KW

   Cellular_componentcell wall

Inferred from electronic annotation. Source: UniProtKB-SubCell

extracellular region

Inferred from electronic annotation. Source: UniProtKB-KW

membrane

Inferred from electronic annotation. Source: InterPro

   Molecular_functionserine-type endopeptidase activity

Inferred from electronic annotation. Source: InterPro

Complete GO annotation...

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Signal peptide1 – 3333
Propeptide34 – 187154
PRO_0000027095
Chain188 – 19301743PIII-type proteinase
PRO_0000027096
Propeptide1931 – 196232Removed by sortase Potential
PRO_0000027097

Regions

Domain1589 – 164153FIVAR 1
Domain1691 – 174252FIVAR 2
Motif1927 – 19315LPXTG sorting signal Potential

Sites

Active site2171Charge relay system By similarity
Active site2811Charge relay system By similarity
Active site6201Charge relay system By similarity

Amino acid modifications

Modified residue19301Pentaglycyl murein peptidoglycan amidated threonine Potential

Experimental info

Sequence conflict1091T → I in AAA03533. Ref.1
Sequence conflict2491A → V in AAA03533. Ref.1
Sequence conflict2761T → K in AAA03533. Ref.1
Sequence conflict3181T → S in AAA03533. Ref.1
Sequence conflict3251T → K in AAA03533. Ref.1
Sequence conflict3311L → V in AAA03533. Ref.1
Sequence conflict3531D → N in AAA03533. Ref.1
Sequence conflict8991E → D in AAA03533. Ref.1
Sequence conflict12131T → M in AAA03533. Ref.1
Sequence conflict12511D → H in AAA03533. Ref.1
Sequence conflict12821E → K in AAA03533. Ref.1
Sequence conflict13041N → K in AAA03533. Ref.1
Sequence conflict13131T → K in AAA03533. Ref.1
Sequence conflict1316 – 13183ANE → TNK in AAA03533. Ref.1
Sequence conflict13941T → S in AAA03533. Ref.1
Sequence conflict14481V → E in AAA03533. Ref.1
Sequence conflict15151K → Q in AAA03533. Ref.1
Sequence conflict15231S → A in AAA03533. Ref.1
Sequence conflict15501V → I in AAA03533. Ref.1
Sequence conflict16731A → D in AAA03533. Ref.1
Sequence conflict16851S → P in AAA03533. Ref.1
Sequence conflict17191V → I in AAA03533. Ref.1
Sequence conflict17221A → E in AAA03533. Ref.1
Sequence conflict1859 – 191860Missing in AAA03533. Ref.1

Sequences

Sequence LengthMass (Da)Tools
P15292 [UniParc].

Last modified January 9, 2007. Version 2.
Checksum: 39B5FD18598DC5CC

FASTA1,962205,996
        10         20         30         40         50         60 
MQRKKKGLSI LLAGTVALGA LAVLPVGEIQ AKAAISQQTK GSSLANTVTA ATAKQAATDT 

        70         80         90        100        110        120 
TAATTNQAIA TQLAAKGIDY NKLNKVQQQD IYVDVIVQMS AAPASENGTL RTDYSSTAEI 

       130        140        150        160        170        180 
QQETNKVIAA QASVKAAVEQ VTQQTAGESY GYVVNGFSTK VRVVDIPKLK QIAGVKTVTL 

       190        200        210        220        230        240 
AKVYYPTDAK ANSMANVQAV WSNYKYKGEG TVVSVIDSGI DPTHKDMRLS DDKDVKLTKS 

       250        260        270        280        290        300 
DVEKFTDTAK HGRYFNSKVP YGFNYADNND TITDDTVDEQ HGMHVAGIIG ANGTGDDPAK 

       310        320        330        340        350        360 
SVVGVAPEAQ LLAMKVFTNS DTSATTGSAT LVSAIEDSAK IGADVLNMSL GSDSGNQTLE 

       370        380        390        400        410        420 
DPELAAVQNA NESGTAAVIS AGNSGTSGSA TEGVNKDYYG LQDNEMVGSP GTSRGATTVA 

       430        440        450        460        470        480 
SAENTDVITQ AVTITDGTGL QLGPETIQLS SHDFTGSFDQ KKFYIVKDAS GNLSKGALAD 

       490        500        510        520        530        540 
YTADAKGKIA IVKRGEFSFD DKQKYAQAAG AAGLIIVNTD GTATPMTSIA LTTTFPTFGL 

       550        560        570        580        590        600 
SSVTGQKLVD WVTAHPDDSL GVKITLAMLP NQKYTEDKMS DFTSYGPVSN LSFKPDITAP 

       610        620        630        640        650        660 
GGNIWSTQNN NGYTNMSGTS MASPFIAGSQ ALLKQALNNK NNPFYAYYKQ LKGTALTDFL 

       670        680        690        700        710        720 
KTVEMNTAQP INDINYNNVI VSPRRQGAGL VDVKAAIDAL EKNPSTVVAE NGYPAVELKD 

       730        740        750        760        770        780 
FTSTDKTFKL TFTNRTTHEL TYQMDSNTDT NAVYTSATDP NSGVLYDKKI DGAAIKAGSN 

       790        800        810        820        830        840 
ITVPAGKTAQ IEFTLSLPKS FDQQQFVEGF LNFKGSDGSR LNLPYMGFFG DWNDGKIVDS 

       850        860        870        880        890        900 
LNGITYSPAG GNFGTVPLLK NKNTGTQYYG GMVTDADGNK TVDDQAIAFS SDKNALYNEI 

       910        920        930        940        950        960 
SMKYYLLRNI SNVQVDILDG QGNKVTTLSS STNRKKTYYN AHSQQYIYYN APAWDGTYYD 

       970        980        990       1000       1010       1020 
QRDGNIKTAD DGSYTYRISG VPEGGDKRQV FDVPFKLDSK APTVRHVALS AKTENGKTQY 

      1030       1040       1050       1060       1070       1080 
YLTAEAKDDL SGLDATKSVK TEINEVTNLD ATFTDAGTTA DGYTKIETPL SDEQAQALGN 

      1090       1100       1110       1120       1130       1140 
GDNSAELYLT DNASNATDQD ASVQKPGSTS FDLIVNGGGI PDKISSTTTG YEANTQGGGT 

      1150       1160       1170       1180       1190       1200 
YTFSGTYPAA VDGTYTDAQG KKHDLNTTYD AATNSFTASM PVTNADYAAQ VDLYADKAHT 

      1210       1220       1230       1240       1250       1260 
QLLKHFDTKV RLTAPTFTDL KFNNGSDQTS EATIKVTGTV SADTKTVNVG DTVAALDAQH 

      1270       1280       1290       1300       1310       1320 
HFSVDVPVNY GDNTIKVTAT DEDGNTTTEQ KTITSSYDPD MLKNSVTFDQ GVTFGANEFN 

      1330       1340       1350       1360       1370       1380 
ATSAKFYDPK TGIATITGKV KHPTTTLQVD GKQIPIKDDL TFSFTLDLGT LGQKPFGVVV 

      1390       1400       1410       1420       1430       1440 
GDTTQNKTFQ EALTFILDAV APTLSLDSST DAPVYTNDPN FQITGTATDN AQYLSLSING 

      1450       1460       1470       1480       1490       1500 
SSVASQYVDI NINSGKPGHM AIDQPVKLLE GKNVLTVAVT DSEDNTTTKN ITVYYEPKKT 

      1510       1520       1530       1540       1550       1560 
LAAPTVTPST TEPAKTVTLT ANSAATGETV QYSADGGKTY QDVPAAGVTV TANGTFKFKS 

      1570       1580       1590       1600       1610       1620 
TDLYGNESPA VDYVVTNIKA DDPAQLQAAK QELTNLIASA KTLSASGKYD DATTTALAAA 

      1630       1640       1650       1660       1670       1680 
TQKAQTALDQ TNASVDSLTG ANRDLQTAIN QLAAKLPADK KTSLLNQLQS VKAALGTDLG 

      1690       1700       1710       1720       1730       1740 
NQTDSSTGKT FTAALDDLVA QAQAGTQTDD QLQATLAKVL DAVLAKLAEG IKAATPAEVG 

      1750       1760       1770       1780       1790       1800 
NAKDAATGKT WYADIADTLT SGQASADASD KLAHLQALQS LKTKVAAAVE AAKTVGKGDG 

      1810       1820       1830       1840       1850       1860 
TTGTSDKGGG QGTPAPAPGD TGKDKGDEGS QPSSGGNIPT KPATTTSTTT DDTTDRNGQL 

      1870       1880       1890       1900       1910       1920 
TSGTSDKGGG QGTPAPAPGD IGKDKGDEGS QPSSGGNIPT NPATTTSTTT DDTTDRNGQL 

      1930       1940       1950       1960 
TSGKGALPKT GETTERPAFG FLGVIVVSLM GVLGLKRKQR EE

« Hide

Cross-references

Sequence databases

EMBL
GenBank
DDBJ		J04962 Unassigned DNA. Translation: AAA03533.1. Sequence problems.
CP000428 Genomic DNA. Translation: ABJ74078.1.
RefSeqYP_796509.1. NC_008505.1.

3D structure databases

ModBaseSearch...

Protein-protein interaction databases

STRING272622.LACR_C42.

Protein family/group databases

MEROPSS08.116.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaABJ74078; ABJ74078; LACR_C42.
GeneID4405830.
KEGGllc:LACR_C42.
PATRIC22286542. VBILacLac38071_0071.

Organism-specific databases

CMRSearch...

Phylogenomic databases

eggNOGCOG1404.
HOGENOMHOG000068537.
KOK01361.
OMATSMASPF.
ProtClustDBCLSK586542.

Enzyme and pathway databases

BioCycLLAC272622:GJUG-2653-MONOMER.
SABIO-RKP15292.

Family and domain databases

Gene3D3.40.50.200. 2 hits.
InterProIPR019948. Gram-positive_anchor.
IPR019931. LPXTG-motif_cell_wall_anchor.
IPR000209. Peptidase_S8/S53_dom.
IPR023827. Peptidase_S8_Asp-AS.
IPR022398. Peptidase_S8_His-AS.
IPR023828. Peptidase_S8_Ser-AS.
IPR015500. Peptidase_S8_subtilisin-rel.
IPR010435. Peptidase_S8A_DUF1034.
IPR003137. Protease-assoc_domain.
[Graphical view]
PANTHERPTHR10795. PTHR10795. 1 hit.
PfamPF06280. DUF1034. 1 hit.
PF00746. Gram_pos_anchor. 1 hit.
PF02225. PA. 1 hit.
PF00082. Peptidase_S8. 1 hit.
[Graphical view]
PRINTSPR00723. SUBTILISIN.
SUPFAMSSF52743. Pept_S8_S53. 1 hit.
TIGRFAMsTIGR01167. LPXTG_anchor. 1 hit.
PROSITEPS50847. GRAM_POS_ANCHORING. 1 hit.
PS00136. SUBTILASE_ASP. 1 hit.
PS00137. SUBTILASE_HIS. 1 hit.
PS00138. SUBTILASE_SER. 1 hit.
[Graphical view]
ProtoNetSearch...

Entry information

Entry nameP3P_LACLS
AccessionPrimary (citable) accession number: P15292
Secondary accession number(s): Q02VE4
Entry history
Integrated into UniProtKB/Swiss-Prot: April 1, 1990
Last sequence update: January 9, 2007
Last modified: May 1, 2013
This is version 107 of the entry and version 2 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Relevant documents

Peptidase families

Classification of peptidase families and list of entries

SIMILARITY comments

Index of protein domains and families

to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·Documents