P15290 (GER2_WHEAT) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names	Recommended name: Oxalate oxidase GF-2.8 EC=1.2.3.4 Alternative name(s): Germin GF-2.8
Organism	Triticum aestivum (Wheat)
Taxonomic identifier	4565 [NCBI]
Taxonomic lineage	Eukaryota › Viridiplantae › Streptophyta › Embryophyta › Tracheophyta › Spermatophyta › Magnoliophyta › Liliopsida › Poales › Poaceae › BEP clade › Pooideae › Triticeae › Triticum

Protein attributes

Sequence length	224 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	Produces developmental and stress-related release of hydrogen peroxide in the apoplast. May play an important role in several aspects of plant growth and defense mechanisms.
Catalytic activity	Oxalate + O2 + 2 H+ = 2 CO2 + H2O2.
Subunit structure	Oligomer (believed to be a pentamer but probably hexamer).
Subcellular location	Secreted › extracellular space › apoplast. Cytoplasm. Secreted › cell wall. Note: Found in the apoplast and the cytoplasm of germinating embryo cells. Associated with the cell wall.
Induction	By auxin, pathogens and heavy metal ions.
Miscellaneous	Associated mostly with highly substituted forms of glucuronogalactoarabinoxylans.
Sequence similarities	Belongs to the germin family.

Ontologies

Keywords
Cellular component	Apoplast Cell wall Cytoplasm Secreted
Domain	Signal
Ligand	Manganese Metal-binding
Molecular function	Oxidoreductase
PTM	Disulfide bond Glycoprotein
Gene Ontology (GO)
Cellular component	apoplast Inferred from electronic annotation. Source: UniProtKB-SubCell cell wall Inferred from electronic annotation. Source: UniProtKB-SubCell cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	manganese ion binding Inferred from electronic annotation. Source: InterPro nutrient reservoir activity Inferred from electronic annotation. Source: InterPro oxalate oxidase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Signal peptide	1 – 23	23
	Chain	24 – 224	201	Oxalate oxidase GF-2.8		PRO_0000010834
Sites
	Metal binding	111	1	Manganese By similarity
	Metal binding	113	1	Manganese By similarity
	Metal binding	118	1	Manganese By similarity
	Metal binding	160	1	Manganese By similarity
Amino acid modifications
	Glycosylation	70	1	N-linked (GlcNAc...) Potential
	Glycosylation	75	1	N-linked (GlcNAc...) Potential
	Disulfide bond	33 ↔ 49		By similarity

Sequences

	Sequence		Length	Mass (Da)
	P15290 [UniParc]. Last modified April 1, 1990. Version 1. Checksum: 2D6DCAC3362CE962	FASTA	224	23,612
	10 20 30 40 50 60 MGYSKTLVAG LFAMLLLAPA VLATDPDPLQ DFCVADLDGK AVSVNGHTCK PMSEAGDDFL 70 80 90 100 110 120 FSSKLAKAGN TSTPNGSAVT ELDVAEWPGT NTLGVSMNRV DFAPGGTNPP HIHPRATEIG 130 140 150 160 170 180 IVMKGELLVG ILGSLDSGNK LYSRVVRAGE TFLIPRGLMH FQFNVGKTEA SMVVSFNSQN 190 200 210 220 PGIVFVPLTL FGSNPPIPTP VLTKALRVEA RVVELLKSKF AAGF

References

[1]	"Homologies between members of the germin gene family in hexaploid wheat and similarities between these wheat germins and certain Physarum spherulins." Lane B.G., Bernier F., Dratewka-Kos E., Shafai R., Kennedy T.D., Pyne C., Munro J.R., Vaughan T., Walters D., Altomare F. J. Biol. Chem. 266:10461-10469(1991) [PubMed: 2037593] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
[2]	"Polypeptide structure of germin as deduced from cDNA sequencing." Dratewka-Kos E., Rahman S., Grzekzak Z.F., Kennedy T.D., Murray R.K., Lane B.G. J. Biol. Chem. 264:4896-4900(1989) [PubMed: 2925674] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA].
[3]	"Regulation by biotic and abiotic stress of a wheat germin gene encoding oxalate oxidase, a H2O2-producing enzyme." Berna A., Bernier F. Plant Mol. Biol. 39:539-549(1999) [PubMed: 10092181] [Abstract] Cited for: CHARACTERIZATION.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	M21962 mRNA. Translation: AAA34268.1. M63223 Genomic DNA. Translation: AAA34270.1.
PIR	A33268. A40391.
UniGene	Ta.56254.
3D structure databases
ProteinModelPortal	P15290.
SMR	P15290. Positions 24-223.
ModBase	Search...
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Organism-specific databases
Gramene	P15290.
Family and domain databases
InterPro	IPR006045. Cupin_1. IPR011051. Cupin_RmlC_type. IPR001929. Germin. IPR019780. Germin_Mn-BS. IPR014710. RmlC-like_jellyroll. [Graphical view]
Gene3D	G3DSA:2.60.120.10. RmlC-like_jellyroll. 1 hit.
Pfam	PF00190. Cupin_1. 1 hit. [Graphical view]
PRINTS	PR00325. GERMIN.
SMART	SM00835. Cupin_1. 1 hit. [Graphical view]
SUPFAM	SSF51182. RmlC_like_cupin. 1 hit.
PROSITE	PS00725. GERMIN. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

GER2_WHEAT

Accession

Primary (citable) accession number: P15290

Entry history

Integrated into UniProtKB/Swiss-Prot:	April 1, 1990
Last sequence update:	April 1, 1990
Last modified:	June 28, 2011
This is version 78 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Plant Protein Annotation Program