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Protein

Cytosol non-specific dipeptidase

Gene

pepD

Organism
Escherichia coli (strain K12)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Dipeptidase with broad substrate specificity. Requires dipeptide substrates with an unblocked N-terminus and the amino group in the alpha or beta position. Non-protein amino acids and proline are not accepted in the C-terminal position, whereas some dipeptide amides and formyl amino acids are hydrolyzed. Also shows cysteinylglycinase activity, which is sufficient for E.coli to utilize cysteinylglycine as a cysteine source.4 Publications

Catalytic activityi

Hydrolysis of dipeptides, preferentially hydrophobic dipeptides including prolyl amino acids.2 Publications

Cofactori

Zn2+1 Publication, Co2+1 PublicationNote: Binds 2 Zn2+ ions per subunit. Can also use Co2+.1 Publication

Enzyme regulationi

Inhibited by metal chelators.1 Publication

pH dependencei

Optimum pH is 9.1 Publication

Temperature dependencei

Optimum temperature is 37 degrees Celsius.1 Publication

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Metal bindingi76Zinc 2By similarity1
Active sitei78By similarity1
Metal bindingi115Zinc 1By similarity1
Metal bindingi115Zinc 2By similarity1
Active sitei145Proton acceptorBy similarity1
Metal bindingi146Zinc 1By similarity1
Metal bindingi169Zinc 2By similarity1
Metal bindingi457Zinc 1By similarity1

GO - Molecular functioni

  • alanylglutamate dipeptidase activity Source: UniProtKB-EC
  • cytosolic dipeptidase activity Source: UniProtKB-EC
  • dipeptidase activity Source: EcoCyc
  • metallodipeptidase activity Source: EcoCyc
  • zinc ion binding Source: EcoCyc

GO - Biological processi

  • peptide catabolic process Source: EcoliWiki

Keywordsi

Molecular functionDipeptidase, Hydrolase, Metalloprotease, Protease
LigandCobalt, Metal-binding, Zinc

Enzyme and pathway databases

BioCyciEcoCyc:EG10695-MONOMER
MetaCyc:EG10695-MONOMER

Protein family/group databases

MEROPSiM20.007

Names & Taxonomyi

Protein namesi
Recommended name:
Cytosol non-specific dipeptidase (EC:3.4.13.18)
Alternative name(s):
Aminoacyl-histidine dipeptidase
Beta-alanyl-histidine dipeptidase
Carnosinase
Cysteinylglycinase
Peptidase D
Xaa-His dipeptidase
Short name:
X-His dipeptidase
Gene namesi
Name:pepD
Synonyms:pepH
Ordered Locus Names:b0237, JW0227
OrganismiEscherichia coli (strain K12)
Taxonomic identifieri83333 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaEnterobacteralesEnterobacteriaceaeEscherichia
Proteomesi
  • UP000000318 Componenti: Chromosome
  • UP000000625 Componenti: Chromosome

Organism-specific databases

EcoGeneiEG10695 pepD

Subcellular locationi

GO - Cellular componenti

  • cytosol Source: EcoCyc

Pathology & Biotechi

Disruption phenotypei

A quadruple peptidase disruption (pepA, pepB, pepD and pepN) does not grow in M9 minimal medium, grows better when supplemented with casamino acids (PubMed:20067529).1 Publication

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Initiator methionineiRemoved1 Publication
ChainiPRO_00001853542 – 485Cytosol non-specific dipeptidaseAdd BLAST484

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei296N6-acetyllysine1 Publication1

Keywords - PTMi

Acetylation

Proteomic databases

EPDiP15288
PaxDbiP15288
PRIDEiP15288

2D gel databases

SWISS-2DPAGEiP15288

PTM databases

iPTMnetiP15288

Interactioni

Binary interactionsi

Show more details

Protein-protein interaction databases

BioGridi425976543 interactors.
DIPiDIP-10456N
IntActiP15288 69 interactors.
STRINGi316385.ECDH10B_0219

Structurei

3D structure databases

ProteinModelPortaliP15288
SMRiP15288
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the peptidase M20C family.Curated

Phylogenomic databases

eggNOGiENOG4105CIN Bacteria
COG2195 LUCA
HOGENOMiHOG000282306
InParanoidiP15288
KOiK01270
OMAiKGGYPGW
PhylomeDBiP15288

Family and domain databases

CDDicd03890 M20_pepD, 1 hit
InterProiView protein in InterPro
IPR002933 Peptidase_M20
IPR011650 Peptidase_M20_dimer
IPR001160 Peptidase_M20C
PANTHERiPTHR43501 PTHR43501, 1 hit
PfamiView protein in Pfam
PF07687 M20_dimer, 1 hit
PF01546 Peptidase_M20, 1 hit
PIRSFiPIRSF016599 Xaa-His_dipept, 1 hit
PRINTSiPR00934 XHISDIPTASE
TIGRFAMsiTIGR01893 aa-his-dipept, 1 hit

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

P15288-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MSELSQLSPQ PLWDIFAKIC SIPHPSYHEE QLAEYIVGWA KEKGFHVERD
60 70 80 90 100
QVGNILIRKP ATAGMENRKP VVLQAHLDMV PQKNNDTVHD FTKDPIQPYI
110 120 130 140 150
DGEWVKARGT TLGADNGIGM ASALAVLADE NVVHGPLEVL LTMTEEAGMD
160 170 180 190 200
GAFGLQGNWL QADILINTDS EEEGEIYMGC AGGIDFTSNL HLDREAVPAG
210 220 230 240 250
FETFKLTLKG LKGGHSGGEI HVGLGNANKL LVRFLAGHAE ELDLRLIDFN
260 270 280 290 300
GGTLRNAIPR EAFATIAVAA DKVDVLKSLV NTYQEILKNE LAEKEKNLAL
310 320 330 340 350
LLDSVANDKA ALIAKSRDTF IRLLNATPNG VIRNSDVAKG VVETSLNVGV
360 370 380 390 400
VTMTDNNVEI HCLIRSLIDS GKDYVVSMLD SLGKLAGAKT EAKGAYPGWQ
410 420 430 440 450
PDANSPVMHL VRETYQRLFN KTPNIQIIHA GLECGLFKKP YPEMDMVSIG
460 470 480
PTITGPHSPD EQVHIESVGH YWTLLTELLK EIPAK
Length:485
Mass (Da):52,915
Last modified:January 23, 2007 - v3
Checksum:iCD870032876460E1
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
M34034 Unassigned DNA Translation: AAA16111.1
U70214 Genomic DNA Translation: AAB08657.1
U00096 Genomic DNA Translation: AAC73341.1
AP009048 Genomic DNA Translation: BAA77906.1
X14790 Genomic DNA Translation: CAA32892.1
PIRiJU0300
RefSeqiNP_414772.1, NC_000913.3
WP_001292994.1, NZ_LN832404.1

Genome annotation databases

EnsemblBacteriaiAAC73341; AAC73341; b0237
BAA77906; BAA77906; BAA77906
GeneIDi945013
KEGGiecj:JW0227
eco:b0237
PATRICifig|1411691.4.peg.2046

Similar proteinsi

Entry informationi

Entry nameiPEPD_ECOLI
AccessioniPrimary (citable) accession number: P15288
Entry historyiIntegrated into UniProtKB/Swiss-Prot: April 1, 1990
Last sequence update: January 23, 2007
Last modified: March 28, 2018
This is version 156 of the entry and version 3 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Direct protein sequencing, Reference proteome