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Protein

Flagellar regulator flk

Gene

flk

Organism
Escherichia coli (strain K12)
Status
Reviewed-Annotation score: Annotation score: 2 out of 5-Protein inferred from homologyi

Functioni

Acts as a regulator of flagellar gene expression by modulating the protein level of the anti sigma factor FlgM upon sensing ring completion or hook elongation. Flk could inhibit FlgM secretion by acting as a braking system for the flagellar-associated type III secretion (T3S) system. Plays a role in hindering to flip the flagellar T3S specificity switch from the rod and hook-type substrates to filament-type substrates prior to hook-basal body (HBB) completion possibly by preventing interaction of FliK with FlhB (By similarity).By similarity

GO - Biological processi

Complete GO annotation...

Enzyme and pathway databases

BioCyciEcoCyc:EG10229-MONOMER.
ECOL316407:JW2318-MONOMER.

Names & Taxonomyi

Protein namesi
Recommended name:
Flagellar regulator flk
Alternative name(s):
Fluke
Gene namesi
Name:flk
Synonyms:div
Ordered Locus Names:b2321, JW2318
OrganismiEscherichia coli (strain K12)
Taxonomic identifieri83333 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaEnterobacterialesEnterobacteriaceaeEscherichia
Proteomesi
  • UP000000318 Componenti: Chromosome
  • UP000000625 Componenti: Chromosome

Organism-specific databases

EcoGeneiEG10229. flk.

Subcellular locationi

Topology

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Topological domaini1 – 309309CytoplasmicSequence analysisAdd
BLAST
Transmembranei310 – 33021HelicalSequence analysisAdd
BLAST
Topological domaini331 – 3311PeriplasmicSequence analysis

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Cell inner membrane, Cell membrane, Membrane

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 331331Flagellar regulator flkPRO_0000079923Add
BLAST

Proteomic databases

PaxDbiP15286.

Interactioni

Protein-protein interaction databases

BioGridi4263217. 44 interactions.
DIPiDIP-9448N.
IntActiP15286. 4 interactions.
STRINGi511145.b2321.

Structurei

3D structure databases

ProteinModelPortaliP15286.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the flk family.Curated

Keywords - Domaini

Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiENOG4106WZ1. Bacteria.
ENOG410YMCE. LUCA.
HOGENOMiHOG000122477.
OMAiFTHLVTW.

Family and domain databases

InterProiIPR023597. Flagellar_regulator_Flk.
[Graphical view]
PIRSFiPIRSF020588. Flk. 1 hit.

Sequencei

Sequence statusi: Complete.

P15286-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MIQPISGPPP GQPPGQGDNL PSGTGNQPLS SQQRTSLESL MTKVTSLTQQ
60 70 80 90 100
QRAELWAGIR HDIGLSGDSP LLSRHFPAAE HNLAQRLLAA QKSHSARQLL
110 120 130 140 150
AQLGEYLRLG NNRQAVTDYI RHNFGQTPLN QLSPEQLKTI LTLLQEGKMV
160 170 180 190 200
IPQPQQREAT DRPLLPAEHN ALKQLVTKLA AATGEPSKQI WQSMLELSGV
210 220 230 240 250
KDGELIPAKL FNHLVTWLQA RQTLSQQNTP TLESLQMTLK QPLDASELAA
260 270 280 290 300
LSAYIQQKYG LSAQSSLSSA QAEDILNQLY QRRVKGIDPR VMQPLLNPFP
310 320 330
PMMDTLQNMA TRPALWILLV AIILMLVWLV R
Length:331
Mass (Da):36,668
Last modified:November 1, 1997 - v2
Checksum:i9201E5BF4B9D27FB
GO

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti2 – 21Missing in AAA24307 (PubMed:2681152).Curated

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U00096 Genomic DNA. Translation: AAC75381.1.
AP009048 Genomic DNA. Translation: BAA16178.1.
U76961 Genomic DNA. Translation: AAB36529.1.
M29962 Genomic DNA. Translation: AAA24307.2.
PIRiG65004.
RefSeqiNP_416824.1. NC_000913.3.
WP_000615834.1. NZ_LN832404.1.

Genome annotation databases

EnsemblBacteriaiAAC75381; AAC75381; b2321.
BAA16178; BAA16178; BAA16178.
GeneIDi946776.
KEGGiecj:JW2318.
eco:b2321.
PATRICi32120015. VBIEscCol129921_2417.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U00096 Genomic DNA. Translation: AAC75381.1.
AP009048 Genomic DNA. Translation: BAA16178.1.
U76961 Genomic DNA. Translation: AAB36529.1.
M29962 Genomic DNA. Translation: AAA24307.2.
PIRiG65004.
RefSeqiNP_416824.1. NC_000913.3.
WP_000615834.1. NZ_LN832404.1.

3D structure databases

ProteinModelPortaliP15286.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi4263217. 44 interactions.
DIPiDIP-9448N.
IntActiP15286. 4 interactions.
STRINGi511145.b2321.

Proteomic databases

PaxDbiP15286.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiAAC75381; AAC75381; b2321.
BAA16178; BAA16178; BAA16178.
GeneIDi946776.
KEGGiecj:JW2318.
eco:b2321.
PATRICi32120015. VBIEscCol129921_2417.

Organism-specific databases

EchoBASEiEB0225.
EcoGeneiEG10229. flk.

Phylogenomic databases

eggNOGiENOG4106WZ1. Bacteria.
ENOG410YMCE. LUCA.
HOGENOMiHOG000122477.
OMAiFTHLVTW.

Enzyme and pathway databases

BioCyciEcoCyc:EG10229-MONOMER.
ECOL316407:JW2318-MONOMER.

Miscellaneous databases

PROiP15286.

Family and domain databases

InterProiIPR023597. Flagellar_regulator_Flk.
[Graphical view]
PIRSFiPIRSF020588. Flk. 1 hit.
ProtoNetiSearch...

Entry informationi

Entry nameiFLK_ECOLI
AccessioniPrimary (citable) accession number: P15286
Secondary accession number(s): P77706, Q9JN62
Entry historyi
Integrated into UniProtKB/Swiss-Prot: April 1, 1990
Last sequence update: November 1, 1997
Last modified: September 7, 2016
This is version 103 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Escherichia coli
    Escherichia coli (strain K12): entries and cross-references to EcoGene
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.