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P15285 (PSPB_RABIT) Reviewed, UniProtKB/Swiss-Prot

Last modified February 19, 2014. Version 106. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (1) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Pulmonary surfactant-associated protein B

Short name=SP-B
Alternative name(s):
6 kDa protein
Pulmonary surfactant-associated proteolipid SPL(Phe)
Gene names
Name:SFTPB
Synonyms:SFTP3
OrganismOryctolagus cuniculus (Rabbit) [Reference proteome]
Taxonomic identifier9986 [NCBI]
Taxonomic lineageEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresLagomorphaLeporidaeOryctolagus

Protein attributes

Sequence length370 AA.
Sequence statusComplete.
Sequence processingThe displayed sequence is further processed into a mature form.
Protein existenceEvidence at transcript level

General annotation (Comments)

Function

Pulmonary surfactant-associated proteins promote alveolar stability by lowering the surface tension at the air-liquid interface in the peripheral air spaces. SP-B increases the collapse pressure of palmitic acid to nearly 70 millinewtons per meter.

Subunit structure

Homodimer; disulfide-linked.

Subcellular location

Secretedextracellular spacesurface film.

Miscellaneous

Pulmonary surfactant consists of 90% lipid and 10% protein. There are 4 surfactant-associated proteins: 2 collagenous, carbohydrate-binding glycoproteins (SP-A and SP-D) and 2 small hydrophobic proteins (SP-B and SP-C).

Sequence similarities

Contains 1 saposin A-type domain.

Contains 3 saposin B-type domains.

Ontologies

Keywords
   Biological processGaseous exchange
   Cellular componentSecreted
Surface film
   DomainRepeat
Signal
   PTMDisulfide bond
Glycoprotein
   Technical termComplete proteome
Reference proteome
Gene Ontology (GO)
   Biological_processlipid metabolic process

Inferred from electronic annotation. Source: InterPro

respiratory gaseous exchange

Inferred from electronic annotation. Source: UniProtKB-KW

   Cellular_componentextracellular space

Inferred from electronic annotation. Source: UniProtKB-SubCell

Complete GO annotation...

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Signal peptide1 – 2424 Potential
Propeptide25 – 184160
PRO_0000031653
Chain185 – 26379Pulmonary surfactant-associated protein B
PRO_0000031654
Propeptide264 – 370107
PRO_0000031655

Regions

Domain26 – 6641Saposin A-type
Domain66 – 14883Saposin B-type 1
Domain188 – 26578Saposin B-type 2
Domain284 – 35976Saposin B-type 3

Amino acid modifications

Glycosylation3001N-linked (GlcNAc...) Potential
Disulfide bond70 ↔ 144 By similarity
Disulfide bond73 ↔ 138 By similarity
Disulfide bond101 ↔ 113 By similarity
Disulfide bond192 ↔ 261 By similarity
Disulfide bond195 ↔ 255 By similarity
Disulfide bond219 ↔ 230 By similarity
Disulfide bond232Interchain By similarity
Disulfide bond288 ↔ 355 By similarity
Disulfide bond291 ↔ 349 By similarity
Disulfide bond314 ↔ 324 By similarity

Experimental info

Sequence conflict151Missing in AAA67934. Ref.2
Sequence conflict1291Q → L in AAB48076. Ref.3
Sequence conflict1841R → P in AAA31466. Ref.1
Sequence conflict2321C → R in AAA67934. Ref.2
Sequence conflict2891R → P in AAB48076. Ref.3
Sequence conflict329 – 35527ELHTP…ALGAC → AAHAPAAEPAVQGLGCPRNL PGPEGRV in AAA31466. Ref.1

Sequences

Sequence LengthMass (Da)Tools
P15285 [UniParc].

Last modified October 1, 1996. Version 2.
Checksum: 423047A69B12DCB5

FASTA37040,610
        10         20         30         40         50         60 
MAKSHLPPWL LLLLLPTLCG PGTAVWATSP LACAQGPEFW CQSLEQALQC KALGHCLQEV 

        70         80         90        100        110        120 
WGHVGADDLC QECQDIVNIL TKMTKEAIFQ DTIRKFLEHE CDVLPLKLLV PQCHHVLDVY 

       130        140        150        160        170        180 
FPLTITYFQS QINAKAICQH LGLCQPGSPE PPLDPLPDKL VLPTLLGALP AKPGPHTQDL 

       190        200        210        220        230        240 
SAQRFPIPLP LCWLCRTLLK RIQAMIPKGV LAMAVAQVCH VVPLVVGGIC QCLAERYTVI 

       250        260        270        280        290        300 
LLEVLLGHVL PQLVCGLVLR CSSVDSIGQV PPTLEALPGE WLPQDPECRL CMSVTTQARN 

       310        320        330        340        350        360 
ISEQTRPQAV YHACLSSQLD KQECEQFVEL HTPQLLSLLS RGWDARAICQ ALGACVATLS 

       370 
PLQCIQSPHF 

« Hide

References

[1]"Isolation and characterization of the cDNA for pulmonary surfactant-associated protein-B (SP-B) in the rabbit."
Xu J., Richardson C., Ford C., Spencer T., Li-Juan Y., Mackie G., Hammond G., Possmayer F.
Biochem. Biophys. Res. Commun. 160:325-332(1989) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA].
Tissue: Lung.
[2]"Transcription and mRNA stability regulate developmental and hormonal expression of rabbit surfactant protein B gene."
Margana R.K., Boggaram V.
Am. J. Physiol. 268:L481-L490(1995) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA].
Strain: New Zealand white.
[3]"Rabbit surfactant protein B gene: structure and functional characterization of the promoter."
Margana R.K., Boggaram V.
Am. J. Physiol. 270:L601-L612(1996) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
Tissue: Liver.
[4]"The upstream region of the SP-B gene: intrinsic promoter activity and glucocorticoid responsiveness related to a new DNA-binding protein."
Luzi P., Anceschi M., Strayer D.S.
Gene 165:285-290(1995) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 1-34.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
M24901 mRNA. Translation: AAA31466.1.
U17106 mRNA. Translation: AAA67934.1.
U40853 Genomic DNA. Translation: AAB48076.1.
S80649 Genomic DNA. Translation: AAD14335.1.
PIRLNRBB. A32421.
I46531.
RefSeqNP_001075812.1. NM_001082343.1.
UniGeneOcu.1963.

3D structure databases

ProteinModelPortalP15285.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

STRING9986.ENSOCUP00000025658.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblENSOCUT00000021287; ENSOCUP00000025658; ENSOCUG00000020733.
GeneID100009194.

Organism-specific databases

CTD100009194.

Phylogenomic databases

eggNOGNOG286450.
GeneTreeENSGT00530000063434.
HOGENOMHOG000115745.
HOVERGENHBG006905.
OMACWLCRTL.
TreeFamTF316942.

Family and domain databases

Gene3D1.10.225.10. 3 hits.
InterProIPR003119. SapA.
IPR007856. SapB_1.
IPR008138. SapB_2.
IPR011001. Saposin-like.
IPR008139. SaposinB.
[Graphical view]
PfamPF02199. SapA. 1 hit.
PF05184. SapB_1. 1 hit.
PF03489. SapB_2. 2 hits.
[Graphical view]
SMARTSM00162. SAPA. 1 hit.
SM00741. SapB. 3 hits.
[Graphical view]
SUPFAMSSF47862. SSF47862. 3 hits.
PROSITEPS51110. SAP_A. 1 hit.
PS50015. SAP_B. 3 hits.
[Graphical view]
ProtoNetSearch...

Entry information

Entry namePSPB_RABIT
AccessionPrimary (citable) accession number: P15285
Secondary accession number(s): P79333
Entry history
Integrated into UniProtKB/Swiss-Prot: April 1, 1990
Last sequence update: October 1, 1996
Last modified: February 19, 2014
This is version 106 of the entry and version 2 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families