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Protein

Pulmonary surfactant-associated protein B

Gene

SFTPB

Organism
Oryctolagus cuniculus (Rabbit)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Experimental evidence at transcript leveli

Functioni

Pulmonary surfactant-associated proteins promote alveolar stability by lowering the surface tension at the air-liquid interface in the peripheral air spaces. SP-B increases the collapse pressure of palmitic acid to nearly 70 millinewtons per meter.

GO - Biological processi

  1. lipid metabolic process Source: InterPro
  2. respiratory gaseous exchange Source: UniProtKB-KW
Complete GO annotation...

Keywords - Biological processi

Gaseous exchange

Names & Taxonomyi

Protein namesi
Recommended name:
Pulmonary surfactant-associated protein B
Short name:
SP-B
Alternative name(s):
6 kDa protein
Pulmonary surfactant-associated proteolipid SPL(Phe)
Gene namesi
Name:SFTPB
Synonyms:SFTP3
OrganismiOryctolagus cuniculus (Rabbit)
Taxonomic identifieri9986 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresLagomorphaLeporidaeOryctolagus
ProteomesiUP000001811: Chromosome 2

Subcellular locationi

GO - Cellular componenti

  1. cytoplasm Source: Ensembl
  2. extracellular space Source: UniProtKB-SubCell
Complete GO annotation...

Keywords - Cellular componenti

Secreted, Surface film

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Signal peptidei1 – 2424Sequence AnalysisAdd
BLAST
Propeptidei25 – 184160PRO_0000031653Add
BLAST
Chaini185 – 26379Pulmonary surfactant-associated protein BPRO_0000031654Add
BLAST
Propeptidei264 – 370107PRO_0000031655Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Disulfide bondi70 ↔ 144PROSITE-ProRule annotation
Disulfide bondi73 ↔ 138PROSITE-ProRule annotation
Disulfide bondi101 ↔ 113PROSITE-ProRule annotation
Disulfide bondi192 ↔ 261PROSITE-ProRule annotation
Disulfide bondi195 ↔ 255PROSITE-ProRule annotation
Disulfide bondi219 ↔ 230PROSITE-ProRule annotation
Disulfide bondi232 – 232InterchainPROSITE-ProRule annotation
Disulfide bondi288 ↔ 355PROSITE-ProRule annotation
Disulfide bondi291 ↔ 349PROSITE-ProRule annotation
Glycosylationi300 – 3001N-linked (GlcNAc...)PROSITE-ProRule annotation
Disulfide bondi314 ↔ 324PROSITE-ProRule annotation

Keywords - PTMi

Disulfide bond, Glycoprotein

Interactioni

Subunit structurei

Homodimer; disulfide-linked.

Protein-protein interaction databases

STRINGi9986.ENSOCUP00000025658.

Structurei

3D structure databases

ProteinModelPortaliP15285.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini26 – 6641Saposin A-typePROSITE-ProRule annotationAdd
BLAST
Domaini66 – 14883Saposin B-type 1PROSITE-ProRule annotationAdd
BLAST
Domaini188 – 26578Saposin B-type 2PROSITE-ProRule annotationAdd
BLAST
Domaini284 – 35976Saposin B-type 3PROSITE-ProRule annotationAdd
BLAST

Sequence similaritiesi

Contains 1 saposin A-type domain.PROSITE-ProRule annotation
Contains 3 saposin B-type domains.PROSITE-ProRule annotation

Keywords - Domaini

Repeat, Signal

Phylogenomic databases

eggNOGiNOG286450.
GeneTreeiENSGT00530000063434.
HOGENOMiHOG000115745.
HOVERGENiHBG006905.
InParanoidiP15285.
OMAiCWLCRTL.
TreeFamiTF316942.

Family and domain databases

Gene3Di1.10.225.10. 3 hits.
InterProiIPR003119. SapA.
IPR007856. SapB_1.
IPR008138. SapB_2.
IPR011001. Saposin-like.
IPR008139. SaposinB.
[Graphical view]
PfamiPF02199. SapA. 1 hit.
PF05184. SapB_1. 1 hit.
PF03489. SapB_2. 2 hits.
[Graphical view]
SMARTiSM00162. SAPA. 1 hit.
SM00741. SapB. 3 hits.
[Graphical view]
SUPFAMiSSF47862. SSF47862. 3 hits.
PROSITEiPS51110. SAP_A. 1 hit.
PS50015. SAP_B. 3 hits.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

P15285-1 [UniParc]FASTAAdd to Basket

« Hide

        10         20         30         40         50
MAKSHLPPWL LLLLLPTLCG PGTAVWATSP LACAQGPEFW CQSLEQALQC
60 70 80 90 100
KALGHCLQEV WGHVGADDLC QECQDIVNIL TKMTKEAIFQ DTIRKFLEHE
110 120 130 140 150
CDVLPLKLLV PQCHHVLDVY FPLTITYFQS QINAKAICQH LGLCQPGSPE
160 170 180 190 200
PPLDPLPDKL VLPTLLGALP AKPGPHTQDL SAQRFPIPLP LCWLCRTLLK
210 220 230 240 250
RIQAMIPKGV LAMAVAQVCH VVPLVVGGIC QCLAERYTVI LLEVLLGHVL
260 270 280 290 300
PQLVCGLVLR CSSVDSIGQV PPTLEALPGE WLPQDPECRL CMSVTTQARN
310 320 330 340 350
ISEQTRPQAV YHACLSSQLD KQECEQFVEL HTPQLLSLLS RGWDARAICQ
360 370
ALGACVATLS PLQCIQSPHF
Length:370
Mass (Da):40,610
Last modified:October 1, 1996 - v2
Checksum:i423047A69B12DCB5
GO

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti15 – 151Missing in AAA67934. (PubMed:7900830)Curated
Sequence conflicti129 – 1291Q → L in AAB48076. (PubMed:8928820)Curated
Sequence conflicti184 – 1841R → P in AAA31466. (PubMed:2469419)Curated
Sequence conflicti232 – 2321C → R in AAA67934. (PubMed:7900830)Curated
Sequence conflicti289 – 2891R → P in AAB48076. (PubMed:8928820)Curated
Sequence conflicti329 – 35527ELHTP…ALGAC → AAHAPAAEPAVQGLGCPRNL PGPEGRV in AAA31466. (PubMed:2469419)CuratedAdd
BLAST

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
M24901 mRNA. Translation: AAA31466.1.
U17106 mRNA. Translation: AAA67934.1.
U40853 Genomic DNA. Translation: AAB48076.1.
S80649 Genomic DNA. Translation: AAD14335.1.
PIRiA32421. LNRBB.
I46531.
RefSeqiNP_001075812.1. NM_001082343.1.
XP_008250836.1. XM_008252614.1.
XP_008250841.1. XM_008252619.1.
XP_008250852.1. XM_008252630.1.
UniGeneiOcu.1963.

Genome annotation databases

EnsembliENSOCUT00000021287; ENSOCUP00000025658; ENSOCUG00000020733.
GeneIDi100009194.
KEGGiocu:100009194.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
M24901 mRNA. Translation: AAA31466.1.
U17106 mRNA. Translation: AAA67934.1.
U40853 Genomic DNA. Translation: AAB48076.1.
S80649 Genomic DNA. Translation: AAD14335.1.
PIRiA32421. LNRBB.
I46531.
RefSeqiNP_001075812.1. NM_001082343.1.
XP_008250836.1. XM_008252614.1.
XP_008250841.1. XM_008252619.1.
XP_008250852.1. XM_008252630.1.
UniGeneiOcu.1963.

3D structure databases

ProteinModelPortaliP15285.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi9986.ENSOCUP00000025658.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSOCUT00000021287; ENSOCUP00000025658; ENSOCUG00000020733.
GeneIDi100009194.
KEGGiocu:100009194.

Organism-specific databases

CTDi6439.

Phylogenomic databases

eggNOGiNOG286450.
GeneTreeiENSGT00530000063434.
HOGENOMiHOG000115745.
HOVERGENiHBG006905.
InParanoidiP15285.
OMAiCWLCRTL.
TreeFamiTF316942.

Family and domain databases

Gene3Di1.10.225.10. 3 hits.
InterProiIPR003119. SapA.
IPR007856. SapB_1.
IPR008138. SapB_2.
IPR011001. Saposin-like.
IPR008139. SaposinB.
[Graphical view]
PfamiPF02199. SapA. 1 hit.
PF05184. SapB_1. 1 hit.
PF03489. SapB_2. 2 hits.
[Graphical view]
SMARTiSM00162. SAPA. 1 hit.
SM00741. SapB. 3 hits.
[Graphical view]
SUPFAMiSSF47862. SSF47862. 3 hits.
PROSITEiPS51110. SAP_A. 1 hit.
PS50015. SAP_B. 3 hits.
[Graphical view]
ProtoNetiSearch...

Publicationsi

  1. "Isolation and characterization of the cDNA for pulmonary surfactant-associated protein-B (SP-B) in the rabbit."
    Xu J., Richardson C., Ford C., Spencer T., Li-Juan Y., Mackie G., Hammond G., Possmayer F.
    Biochem. Biophys. Res. Commun. 160:325-332(1989) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA].
    Tissue: Lung.
  2. "Transcription and mRNA stability regulate developmental and hormonal expression of rabbit surfactant protein B gene."
    Margana R.K., Boggaram V.
    Am. J. Physiol. 268:L481-L490(1995) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA].
    Strain: New Zealand white.
  3. "Rabbit surfactant protein B gene: structure and functional characterization of the promoter."
    Margana R.K., Boggaram V.
    Am. J. Physiol. 270:L601-L612(1996) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
    Tissue: Liver.
  4. "The upstream region of the SP-B gene: intrinsic promoter activity and glucocorticoid responsiveness related to a new DNA-binding protein."
    Luzi P., Anceschi M., Strayer D.S.
    Gene 165:285-290(1995) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 1-34.

Entry informationi

Entry nameiPSPB_RABIT
AccessioniPrimary (citable) accession number: P15285
Secondary accession number(s): P79333
Entry historyi
Integrated into UniProtKB/Swiss-Prot: April 1, 1990
Last sequence update: October 1, 1996
Last modified: February 4, 2015
This is version 110 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Miscellaneous

Pulmonary surfactant consists of 90% lipid and 10% protein. There are 4 surfactant-associated proteins: 2 collagenous, carbohydrate-binding glycoproteins (SP-A and SP-D) and 2 small hydrophobic proteins (SP-B and SP-C).

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.