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P15284 (MBHL_RHOCA) Reviewed, UniProtKB/Swiss-Prot

Last modified September 21, 2011. Version 71. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (1) | Third-party data text xml rdf/xml gff fasta
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Names and origin

Protein namesRecommended name:
Uptake hydrogenase large subunit
EC=1.12.99.6
Alternative name(s):
Hydrogenlyase
Membrane-bound hydrogenase large subunit
Gene names
Name:hupB
Synonyms:hupL
OrganismRhodobacter capsulatus (Rhodopseudomonas capsulata)
Taxonomic identifier1061 [NCBI]
Taxonomic lineageBacteria › Proteobacteria › Alphaproteobacteria › Rhodobacterales › Rhodobacteraceae › Rhodobacter

Protein attributes

Sequence length597 AA.
Sequence statusComplete.
Protein existenceInferred from homology

General annotation (Comments)

Function

This enzyme recycles the H2 produced by nitrogenase to increase the production of ATP and to protect nitrogenase against inhibition or damage by O2 under carbon- or phosphate-limited conditions.

Catalytic activity

H2 + A = AH2.

Cofactor

Binds 1 nickel ion per subunit By similarity.

Subunit structure

Heterodimer of a large and a small subunit.

Subcellular location

Cell membrane; Peripheral membrane protein.

Sequence similarities

Belongs to the [NiFe]/[NiFeSe] hydrogenase large subunit family.

Ontologies

Keywords
   Cellular componentCell membrane
Membrane
   LigandMetal-binding
Nickel
   Molecular functionOxidoreductase
Gene Ontology (GO)
   Cellular componentplasma membrane

Inferred from electronic annotation. Source: UniProtKB-SubCell

   Molecular functionferredoxin hydrogenase activity

Inferred from electronic annotation. Source: InterPro

hydrogenase (acceptor) activity

Inferred from electronic annotation. Source: EC

nickel cation binding

Inferred from electronic annotation. Source: InterPro

Complete GO annotation...

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 597597Uptake hydrogenase large subunit
PRO_0000199716

Sites

Metal binding751Nickel Potential
Metal binding781Nickel Potential
Metal binding5761Nickel Potential
Metal binding5791Nickel Potential

Sequences

Sequence LengthMass (Da)Tools
P15284 [UniParc].

Last modified April 1, 1990. Version 1.
Checksum: C038E25E05EFB9C8

FASTA59766,046
        10         20         30         40         50         60 
MTTQTPNGFT LDNAGKRIVV DPVTRIEGHM RCEVNVNDQG IITNAVSTGT MWRGLEVILK 

        70         80         90        100        110        120 
GRDPRDAWAF TERICGVCTG THALTSVRAV ESALGITIPD NANSIRNMMQ LNLQIHDHIV 

       130        140        150        160        170        180 
HFYHLHALDW VNPVNALRAD PKATSELQQM VSPSHPLSSP GYFRDVQNRL KKFVESGQLG 

       190        200        210        220        230        240 
LFKNGYWDNP AYKLPPEADL MATTHYLEAL DLQKEVVKVH TIFGGKNPHP NWLVGGVPCP 

       250        260        270        280        290        300 
INVDGVGAVG AINMERLNLV SSIIDRCTEF TRNVYLPDLK AIGGFYKEWL YGGGLSGQSV 

       310        320        330        340        350        360 
LSYGDIPENP NDFSAGQLHL PRGAIINGNL NEVHDVDTTD PEQVQEFVDH SWYDYGEPGM 

       370        380        390        400        410        420 
GLHPWDGRTE PKFELGPNLK GTRTNIENID EGAKYSWIKA PRWRGNAMEV GPLAATSSVT 

       430        440        450        460        470        480 
RKGHEDIKNQ VEGLLRDMNL PVSALFSTLG RTAARALEAE YCCRLQKHFF DKLVTNIKNG 

       490        500        510        520        530        540 
DSSTANVEKW DPSTWPKEAK GVGMTEAPRG ALGHWVKIKD GRIENYQCVV PTTWNGSPRD 

       550        560        570        580        590 
SKGNIGAFEA SLLNTKMERP EEPVEILRTL HSFDPCLACS THVMSAEGAP LTTVKVR

« Hide

References

[1]"Cloning and sequencing of the genes encoding the large and the small subunits of the H2 uptake hydrogenase (hup) of Rhodobacter capsulatus."
Leclerc M., Colbeau A., Cauvin B., Vignais P.M.
Mol. Gen. Genet. 214:97-107(1988) [PubMed: 3067084] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
Strain: ATCC 33303 / B10.
[2]Erratum
Leclerc M., Colbeau A., Cauvin B., Vignais P.M.
Mol. Gen. Genet. 215:368-368(1989)
Cited for: SEQUENCE REVISION.
+Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ		X13520 Genomic DNA. Translation: CAA31870.1.
PIRS08317.

3D structure databases

ProteinModelPortalP15284.
ModBaseSearch...

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Family and domain databases

InterProIPR001501. Ni-dep_hyd_lsu.
IPR018194. Ni-dep_hyd_lsu_Ni_BS.
[Graphical view]
PfamPF00374. NiFeSe_Hases. 1 hit.
[Graphical view]
PROSITEPS00507. NI_HGENASE_L_1. 1 hit.
PS00508. NI_HGENASE_L_2. 1 hit.
[Graphical view]
ProtoNetSearch...

Entry information

Entry nameMBHL_RHOCA
AccessionPrimary (citable) accession number: P15284
Entry history
Integrated into UniProtKB/Swiss-Prot: April 1, 1990
Last sequence update: April 1, 1990
Last modified: September 21, 2011
This is version 71 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

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