Reviewed, UniProtKB/Swiss-Prot P15284 (MBHL_RHOCA)

Names and origin

Protein names

Recommended name:
    Uptake hydrogenase large subunit
    EC=1.12.99.6
Alternative name(s):
    Hydrogenlyase
    Membrane-bound hydrogenase large subunit

Gene names

Name:	hupB
Synonyms:	hupL

Organism

Rhodobacter capsulatus (Rhodopseudomonas capsulata)

Taxonomic identifier

1061 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Alphaproteobacteria › Rhodobacterales › Rhodobacteraceae › Rhodobacter

Protein attributes

Sequence length	597 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Inferred from homology.

General annotation (Comments)

Function	This enzyme recycles the H2 produced by nitrogenase to increase the production of ATP and to protect nitrogenase against inhibition or damage by O2 under carbon- or phosphate-limited conditions.
Catalytic activity	H2 + A = AH2.
Cofactor	Binds 1 nickel ion per subunit By similarity.
Subunit structure	Heterodimer of a large and a small subunit.
Subcellular location	Cell membrane; Peripheral membrane protein.
Sequence similarities	Belongs to the [NiFe]/[NiFeSe] hydrogenase large subunit family.

Ontologies

Keywords
Cellular component	Cell membrane Membrane
Ligand	Metal-binding Nickel
Molecular function	Oxidoreductase
Gene Ontology (GO)
Biological process	oxidation reduction Inferred from electronic annotation. Source: UniProtKB-KW
Cellular component	plasma membrane Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	ferredoxin hydrogenase activity Inferred from electronic annotation. Source: InterPro hydrogenase (acceptor) activity Inferred from electronic annotation. Source: EC nickel ion binding Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 597	597	Uptake hydrogenase large subunit		PRO_0000199716
Sites
	Metal binding	75	1	Nickel Potential
	Metal binding	78	1	Nickel Potential
	Metal binding	576	1	Nickel Potential
	Metal binding	579	1	Nickel Potential

Sequences

	Sequence		Length	Mass (Da)
	P15284-1 [UniParc]. Last modified April 1, 1990. Version 1. Checksum: C038E25E05EFB9C8	FASTA	597	66,046
	10 20 30 40 50 60 MTTQTPNGFT LDNAGKRIVV DPVTRIEGHM RCEVNVNDQG IITNAVSTGT MWRGLEVILK 70 80 90 100 110 120 GRDPRDAWAF TERICGVCTG THALTSVRAV ESALGITIPD NANSIRNMMQ LNLQIHDHIV 130 140 150 160 170 180 HFYHLHALDW VNPVNALRAD PKATSELQQM VSPSHPLSSP GYFRDVQNRL KKFVESGQLG 190 200 210 220 230 240 LFKNGYWDNP AYKLPPEADL MATTHYLEAL DLQKEVVKVH TIFGGKNPHP NWLVGGVPCP 250 260 270 280 290 300 INVDGVGAVG AINMERLNLV SSIIDRCTEF TRNVYLPDLK AIGGFYKEWL YGGGLSGQSV 310 320 330 340 350 360 LSYGDIPENP NDFSAGQLHL PRGAIINGNL NEVHDVDTTD PEQVQEFVDH SWYDYGEPGM 370 380 390 400 410 420 GLHPWDGRTE PKFELGPNLK GTRTNIENID EGAKYSWIKA PRWRGNAMEV GPLAATSSVT 430 440 450 460 470 480 RKGHEDIKNQ VEGLLRDMNL PVSALFSTLG RTAARALEAE YCCRLQKHFF DKLVTNIKNG 490 500 510 520 530 540 DSSTANVEKW DPSTWPKEAK GVGMTEAPRG ALGHWVKIKD GRIENYQCVV PTTWNGSPRD 550 560 570 580 590 SKGNIGAFEA SLLNTKMERP EEPVEILRTL HSFDPCLACS THVMSAEGAP LTTVKVR

References

[1]	"Cloning and sequencing of the genes encoding the large and the small subunits of the H2 uptake hydrogenase (hup) of Rhodobacter capsulatus." Leclerc M., Colbeau A., Cauvin B., Vignais P.M. Mol. Gen. Genet. 214:97-107(1988) [PubMed: 3067084] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Strain: ATCC 33303 / B10.
[2]	Erratum Leclerc M., Colbeau A., Cauvin B., Vignais P.M. Mol. Gen. Genet. 215:368-368(1989) Cited for: SEQUENCE REVISION.

Cross-references

Sequence databases
EMBLGenBankDDBJ	X13520 Genomic DNA. Translation: CAA31870.1.
PIR	S08317.
3D structure databases
HSSP	HSSP built from PDB template 2FRV based on UniProtKB P12944.
ModBase	Search...
Enzyme and pathway databases
BRENDA	1.12.99.6. 567.
Family and domain databases
InterPro	IPR001501. Ni-dep_hyd_lsu. IPR018194. Ni-dep_hyd_lsu_Ni_BS. [Graphical view]
Pfam	PF00374. NiFeSe_Hases. 1 hit. [Graphical view]
PROSITE	PS00507. NI_HGENASE_L_1. 1 hit. PS00508. NI_HGENASE_L_2. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

MBHL_RHOCA

Accession

Primary (citable) accession number: P15284

Entry history

Integrated into UniProtKB/Swiss-Prot:	April 1, 1990
Last sequence update:	April 1, 1990
Last modified:	June 16, 2009
This is version 60 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)