Reviewed, UniProtKB/Swiss-Prot P15283 (MBHS_RHOCA)

Names and origin

Protein names

Recommended name:
    Uptake hydrogenase small subunit
    EC=1.12.99.6
Alternative name(s):
    Hydrogenlyase
    Membrane-bound hydrogenase small subunit

Gene names

Name:	hupA
Synonyms:	hupS

Organism

Rhodobacter capsulatus (Rhodopseudomonas capsulata)

Taxonomic identifier

1061 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Alphaproteobacteria › Rhodobacterales › Rhodobacteraceae › Rhodobacter

Protein attributes

Sequence length	358 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Inferred from homology.

General annotation (Comments)

Function	This enzyme recycles the H2 produced by nitrogenase to increase the production of ATP and to protect nitrogenase against inhibition or damage by O2 under carbon- or phosphate-limited conditions.
Catalytic activity	H2 + A = AH2.
Cofactor	Binds 1 3Fe-4S cluster By similarity. Binds 2 4Fe-4S clusters By similarity.
Subunit structure	Heterodimer of a large and a small subunit.
Subcellular location	Cell membrane; Peripheral membrane protein.
Sequence similarities	Belongs to the [NiFe]/[NiFeSe] hydrogenase small subunit family.

Ontologies

Keywords
Cellular component	Cell membrane Membrane
Domain	Signal
Ligand	3Fe-4S 4Fe-4S Iron Iron-sulfur Metal-binding
Molecular function	Oxidoreductase
Gene Ontology (GO)
Biological process	oxidation reduction Inferred from electronic annotation. Source: UniProtKB-KW
Cellular component	ferredoxin hydrogenase complex Inferred from electronic annotation. Source: InterPro plasma membrane Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	3 iron, 4 sulfur cluster binding Inferred from electronic annotation. Source: UniProtKB-KW 4 iron, 4 sulfur cluster binding Inferred from electronic annotation. Source: UniProtKB-KW NADH dehydrogenase (ubiquinone) activity Inferred from electronic annotation. Source: InterPro ferredoxin hydrogenase activity Inferred from electronic annotation. Source: InterPro hydrogenase (acceptor) activity Inferred from electronic annotation. Source: EC iron ion binding Inferred from electronic annotation. Source: UniProtKB-KW quinone binding Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Signal peptide	1 – 45	45
	Chain	46 – 358	313	Uptake hydrogenase small subunit		PRO_0000013435
Sites
	Metal binding	62	1	Iron-sulfur 1 (4Fe-4S) By similarity
	Metal binding	65	1	Iron-sulfur 1 (4Fe-4S) By similarity
	Metal binding	160	1	Iron-sulfur 1 (4Fe-4S) By similarity
	Metal binding	194	1	Iron-sulfur 1 (4Fe-4S) By similarity
	Metal binding	232	1	Iron-sulfur 2 (4Fe-4S); via pros nitrogen By similarity
	Metal binding	235	1	Iron-sulfur 2 (4Fe-4S) By similarity
	Metal binding	260	1	Iron-sulfur 2 (4Fe-4S) By similarity
	Metal binding	266	1	Iron-sulfur 2 (4Fe-4S) By similarity
	Metal binding	275	1	Iron-sulfur 3 (3Fe-4S) By similarity
	Metal binding	294	1	Iron-sulfur 3 (3Fe-4S) By similarity
	Metal binding	297	1	Iron-sulfur 3 (3Fe-4S) By similarity

Sequences

	Sequence		Length	Mass (Da)
	P15283-1 [UniParc]. Last modified April 1, 1990. Version 1. Checksum: D08C1887F1376237	FASTA	358	39,403
	10 20 30 40 50 60 MSDIETFYDV MRRQGITRRS FMKSVRSPQH VLGLGPSFVP KIGEAMETKP RTPVVWVHGL 70 80 90 100 110 120 ECTCCSESFI RSAHPLAKDV VLSMISLDYD DTLMAAAGHA AEAAFEETIA KYKGNYILAV 130 140 150 160 170 180 EGNPPLNEDG MFCITGGKPF VEKLRHAAEG AKAIISWGAC ASYGCVQAAA PNPTQATPVH 190 200 210 220 230 240 KVITDKPIIK VPGCPPIAEV MTGVITYMLT FDRMPELDRQ GRPAMFYSQR IHDKCYRRPH 250 260 270 280 290 300 FDAGQFVEHW DDENARKGYC LYKMGCKGPT TYNACSTVPL ERRRHFPIQS GHGCIGCSED 310 320 330 340 350 GFWDQGSFYD RLTTIKQFGI EATADQIGWT ATGLVGAAVA AHAAVSVLKR AQKKNEEA

References

[1]	"Cloning and sequencing of the genes encoding the large and the small subunits of the H2 uptake hydrogenase (hup) of Rhodobacter capsulatus." Leclerc M., Colbeau A., Cauvin B., Vignais P.M. Mol. Gen. Genet. 214:97-107(1988) [PubMed: 3067084] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Strain: ATCC 33303 / B10.
[2]	Erratum Leclerc M., Colbeau A., Cauvin B., Vignais P.M. Mol. Gen. Genet. 215:368-368(1989) Cited for: SEQUENCE REVISION.
[3]	"Organization of the genes necessary for hydrogenase expression in Rhodobacter capsulatus. Sequence analysis and identification of two hyp regulatory mutants." Colbeau A., Richaud P., Toussaint B., Caballero F.J., Elster C., Delphin C., Smith R.L., Chabert J., Vignais P.M. Mol. Microbiol. 8:15-29(1993) [PubMed: 8497190] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 1-18. Strain: ATCC 33303 / B10.

Cross-references

Sequence databases
EMBLGenBankDDBJ	X13520 Genomic DNA. Translation: CAA31869.1. Sequence problems. M37261 Genomic DNA. Translation: AAA69668.1. Different termination.
PIR	S08316.
3D structure databases
HSSP	HSSP built from PDB template 1FRF based on UniProtKB P18187.
ModBase	Search...
Enzyme and pathway databases
BRENDA	1.12.99.6. 567.
Family and domain databases
InterPro	IPR006137. NADH_UbQ_OxRdtase-like_20kDa. IPR001821. NiFe_hydrogenase_ssu. [Graphical view]
Pfam	PF01058. Oxidored_q6. 1 hit. [Graphical view]
PIRSF	PIRSF000310. NiFe_hyd_ssu. 1 hit.
PRINTS	PR00614. NIHGNASESMLL.
TIGRFAMs	TIGR00391. hydA. 1 hit.
ProtoNet	Search...

Entry information

Entry name

MBHS_RHOCA

Accession

Primary (citable) accession number: P15283

Entry history

Integrated into UniProtKB/Swiss-Prot:	April 1, 1990
Last sequence update:	April 1, 1990
Last modified:	June 16, 2009
This is version 67 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)