ID   DHM1_METOR              Reviewed;         626 AA.
AC   P15279;
DT   01-APR-1990, integrated into UniProtKB/Swiss-Prot.
DT   01-APR-1990, sequence version 1.
DT   16-JUN-2009, entry version 75.
DE   RecName: Full=Methanol dehydrogenase subunit 1;
DE            EC=1.1.99.8;
DE   AltName: Full=MDH large subunit alpha;
DE   AltName: Full=MEDH;
DE   Flags: Precursor;
GN   Name=moxF;
OS   Methylobacterium organophilum XX.
OC   Bacteria; Proteobacteria; Alphaproteobacteria; Rhizobiales;
OC   Methylobacteriaceae; Methylobacterium.
OX   NCBI_TaxID=410;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA], AND PROTEIN SEQUENCE OF 28-43.
RC   STRAIN=ATCC 27886 / DSM 760 / IFO 15689 / JCM 2833 / NCIB 11278;
RX   MEDLINE=89008094; PubMed=2459109;
RA   Machlin S.M., Hanson R.S.;
RT   "Nucleotide sequence and transcriptional start site of the
RT   Methylobacterium organophilum XX methanol dehydrogenase structural
RT   gene.";
RL   J. Bacteriol. 170:4739-4747(1988).
CC   -!- CATALYTIC ACTIVITY: A primary alcohol + acceptor = an aldehyde +
CC       reduced acceptor.
CC   -!- COFACTOR: Binds 1 PQQ group per subunit. PQQ is inserted between
CC       disulfide Cys-130-Cys-131 and the indole ring of Trp-270 (By
CC       similarity).
CC   -!- COFACTOR: Binds 1 calcium ion per subunit (By similarity).
CC   -!- SUBUNIT: Heterotetramer composed of 2 alpha and 2 beta subunits.
CC   -!- SUBCELLULAR LOCATION: Cell inner membrane; Peripheral membrane
CC       protein; Periplasmic side. Note=Periplasmic, but associated with
CC       inner membrane.
CC   -!- SIMILARITY: Belongs to the bacterial PQQ dehydrogenase family.
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DR   EMBL; M22629; AAA50289.1; -; Genomic_DNA.
DR   HSSP; P16027; 1H4J.
DR   SMR; P15279; 28-622.
DR   BioCyc; MetaCyc:MON-3927; -.
DR   BRENDA; 1.1.99.8; 190843.
DR   GO; GO:0030288; C:outer membrane-bounded periplasmic space; IEA:InterPro.
DR   GO; GO:0005886; C:plasma membrane; IEA:UniProtKB-SubCell.
DR   GO; GO:0018468; F:alcohol dehydrogenase (acceptor) activity; IEA:EC.
DR   GO; GO:0005509; F:calcium ion binding; IEA:UniProtKB-KW.
DR   GO; GO:0009055; F:electron carrier activity; IEA:InterPro.
DR   GO; GO:0015945; P:methanol metabolic process; IEA:UniProtKB-KW.
DR   GO; GO:0055114; P:oxidation reduction; IEA:UniProtKB-KW.
DR   InterPro; IPR019556; PQQ-dependent_C.
DR   InterPro; IPR019551; PQQ-dependent_N.
DR   InterPro; IPR018391; PQQ_beta_propeller_repeat.
DR   InterPro; IPR017512; PQQ_MeOH/EtOH_DH.
DR   InterPro; IPR002372; PQQ_repeat.
DR   InterPro; IPR011047; Quino_AlcDH-like.
DR   InterPro; IPR001479; Quinoprotein_DH_CS.
DR   Gene3D; G3DSA:2.140.10.10; Quinoprotein_alc_DH-like; 1.
DR   Pfam; PF01011; PQQ; 5.
DR   Pfam; PF10535; PQQ_C; 1.
DR   Pfam; PF10527; PQQ_N; 1.
DR   SMART; SM00564; PQQ; 3.
DR   TIGRFAMs; TIGR03075; PQQ_enz_alc_DH; 1.
DR   PROSITE; PS00363; BACTERIAL_PQQ_1; 1.
DR   PROSITE; PS00364; BACTERIAL_PQQ_2; 1.
PE   1: Evidence at protein level;
KW   Calcium; Cell inner membrane; Cell membrane;
KW   Direct protein sequencing; Disulfide bond; Membrane; Metal-binding;
KW   Methanol utilization; Oxidoreductase; PQQ; Signal.
FT   SIGNAL        1     27
FT   CHAIN        28    626       Methanol dehydrogenase subunit 1.
FT                                /FTId=PRO_0000025567.
FT   ACT_SITE    330    330       Proton acceptor (By similarity).
FT   METAL       204    204       Calcium (By similarity).
FT   METAL       288    288       Calcium (By similarity).
FT   DISULFID    130    131       By similarity.
FT   DISULFID    413    442       By similarity.
SQ   SEQUENCE   626 AA;  68677 MW;  8768F6B8371E5DFF CRC64;
     MSRFVTSVSA LAMLALAPAA LSSVAYANDK LVELSKSDDN WVMPGKNYDS NNYSELKQVN
     KSNVKQLRPA WTFSTGLLNG HEGAPLVVDG KMYVHTSFPN NTFALDLDDP GHILWQDKPK
     QNPAARAVAC CDLVNRGLAY WPGDGKTPAL ILKTQLDRHV VALNAETGET VWKVENSDIK
     VGSTLTIAPY VVKDKVIIGS SGAELGVRGY LTAYDVKTGG QVWRAYATGP DKDLLLADDF
     NVKNAHYGQK GLGTATWEGD AWKIGGGTNW GWYAYDPGTN LIYFGTGNPA PWNETMRPGD
     NKWTMTIFGR DADTGEAKFG YQKTPHDEWD YAGVNVMMPS EQKDKDGKTR KLLTHPDRNG
     IVYTLDRTDG ALVSANKLDD TVNVFKTVDL KTGQPVRDPE YGTRMDHLAK DVCPSAMGYH
     NQGHDSYDPK RELFFMGINH ICMDWEPFML PYRAGQFFVG ATLNMYPGPK GDRQNYEGLG
     QIKAYNAITG SYKWEKMERF AVWGGTLATA GDLVFYGTLD GYLKARDSDT GDLLWKFKIP
     SGAIGYPMTY THKGTQYVAI YYGVGGWPGV GLVFDLADPT AGLGAVGAFK KLANYTQQGG
     GVIVFSLDGK GPYDDPNVGE WKSASK
//