P15279 (DHM1_METOR) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Methanol dehydrogenase [cytochrome c] subunit 1
EC=1.1.2.7

Alternative name(s):
MDH large subunit alpha
MEDH

Gene names

Name:

moxF

Organism

Methylobacterium organophilum

Taxonomic identifier

410 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Alphaproteobacteria › Rhizobiales › Methylobacteriaceae › Methylobacterium

Protein attributes

Sequence length	626 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	Catalyzes the oxidation of primary alcohols including methanol By similarity.
Catalytic activity	A primary alcohol + 2 cytochrome c(L) = an aldehyde + 2 reduced cytochrome c(L).
Cofactor	Binds 1 PQQ group per subunit. PQQ is inserted between disulfide Cys-130-Cys-131 and the indole ring of Trp-270 By similarity. Binds 1 calcium ion per subunit By similarity.
Subunit structure	Heterotetramer composed of 2 alpha and 2 beta subunits.
Subcellular location	Cell inner membrane; Peripheral membrane protein; Periplasmic side. Note: Periplasmic, but associated with inner membrane.
Sequence similarities	Belongs to the bacterial PQQ dehydrogenase family.

Ontologies

Keywords
Biological process	Methanol utilization
Cellular component	Cell inner membrane Cell membrane Membrane
Domain	Signal
Ligand	Calcium Metal-binding PQQ
Molecular function	Oxidoreductase
PTM	Disulfide bond
Technical term	Direct protein sequencing
Gene Ontology (GO)
Biological process	methanol metabolic process Inferred from electronic annotation. Source: UniProtKB-KW
Cellular component	outer membrane-bounded periplasmic space Inferred from electronic annotation. Source: InterPro plasma membrane Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	calcium ion binding Inferred from electronic annotation. Source: InterPro oxidoreductase activity, acting on CH-OH group of donors Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Signal peptide	1 – 27	27	Ref.1
	Chain	28 – 626	599	Methanol dehydrogenase [cytochrome c] subunit 1		PRO_0000025567
Sites
	Active site	330	1	Proton acceptor By similarity
	Metal binding	204	1	Calcium By similarity
	Metal binding	288	1	Calcium By similarity
Amino acid modifications
	Disulfide bond	130 ↔ 131		By similarity
	Disulfide bond	413 ↔ 442		By similarity

Sequences

	Sequence		Length	Mass (Da)
	P15279 [UniParc]. Last modified April 1, 1990. Version 1. Checksum: 8768F6B8371E5DFF	FASTA	626	68,677
	10 20 30 40 50 60 MSRFVTSVSA LAMLALAPAA LSSVAYANDK LVELSKSDDN WVMPGKNYDS NNYSELKQVN 70 80 90 100 110 120 KSNVKQLRPA WTFSTGLLNG HEGAPLVVDG KMYVHTSFPN NTFALDLDDP GHILWQDKPK 130 140 150 160 170 180 QNPAARAVAC CDLVNRGLAY WPGDGKTPAL ILKTQLDRHV VALNAETGET VWKVENSDIK 190 200 210 220 230 240 VGSTLTIAPY VVKDKVIIGS SGAELGVRGY LTAYDVKTGG QVWRAYATGP DKDLLLADDF 250 260 270 280 290 300 NVKNAHYGQK GLGTATWEGD AWKIGGGTNW GWYAYDPGTN LIYFGTGNPA PWNETMRPGD 310 320 330 340 350 360 NKWTMTIFGR DADTGEAKFG YQKTPHDEWD YAGVNVMMPS EQKDKDGKTR KLLTHPDRNG 370 380 390 400 410 420 IVYTLDRTDG ALVSANKLDD TVNVFKTVDL KTGQPVRDPE YGTRMDHLAK DVCPSAMGYH 430 440 450 460 470 480 NQGHDSYDPK RELFFMGINH ICMDWEPFML PYRAGQFFVG ATLNMYPGPK GDRQNYEGLG 490 500 510 520 530 540 QIKAYNAITG SYKWEKMERF AVWGGTLATA GDLVFYGTLD GYLKARDSDT GDLLWKFKIP 550 560 570 580 590 600 SGAIGYPMTY THKGTQYVAI YYGVGGWPGV GLVFDLADPT AGLGAVGAFK KLANYTQQGG 610 620 GVIVFSLDGK GPYDDPNVGE WKSASK

References

[1]	"Nucleotide sequence and transcriptional start site of the Methylobacterium organophilum XX methanol dehydrogenase structural gene." Machlin S.M., Hanson R.S. J. Bacteriol. 170:4739-4747(1988) [PubMed: 2459109] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA], PROTEIN SEQUENCE OF 28-43. Strain: ATCC 27886 / DSM 760 / JCM 2833 / NBRC 15689 / NCIMB 11278 / VKM B-2066 / XX.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	M22629 Genomic DNA. Translation: AAA50289.1.
3D structure databases
ProteinModelPortal	P15279.
SMR	P15279. Positions 28-622.
ModBase	Search...
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Enzyme and pathway databases
BioCyc	MetaCyc:MONOMER-3927.
Family and domain databases
InterPro	IPR019556. PQQ-dependent_C. IPR019551. PQQ-dependent_N. IPR018391. PQQ_beta_propeller_repeat. IPR017512. PQQ_MeOH/EtOH_DH. IPR002372. PQQ_repeat. IPR011047. Quinonprotein_ADH-like. IPR001479. Quinoprotein_DH_CS. [Graphical view]
Gene3D	G3DSA:2.140.10.10. Quinoprotein_alc_DH-like. 1 hit.
Pfam	PF01011. PQQ. 4 hits. PF10535. PQQ_C. 1 hit. PF10527. PQQ_N. 1 hit. [Graphical view]
SMART	SM00564. PQQ. 3 hits. [Graphical view]
SUPFAM	SSF50998. Quin_alc_DH_like. 1 hit.
TIGRFAMs	TIGR03075. PQQ_enz_alc_DH. 1 hit.
PROSITE	PS00363. BACTERIAL_PQQ_1. 1 hit. PS00364. BACTERIAL_PQQ_2. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

DHM1_METOR

Accession

Primary (citable) accession number: P15279

Entry history

Integrated into UniProtKB/Swiss-Prot:	April 1, 1990
Last sequence update:	April 1, 1990
Last modified:	July 27, 2011
This is version 89 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program