Reviewed, UniProtKB/Swiss-Prot P15278 (FAS3_DROME)

Names and origin

Protein names

Recommended name:
    Fasciclin-3
Alternative name(s):
    Fasciclin III
      Short name=FAS III

Gene names

Name:	Fas3
ORF Names:	CG5803

Organism

Drosophila melanogaster (Fruit fly) [Complete proteome]

Taxonomic identifier

7227 [NCBI]

Taxonomic lineage

Eukaryota › Metazoa › Arthropoda › Hexapoda › Insecta › Pterygota › Neoptera › Endopterygota › Diptera › Brachycera › Muscomorpha › Ephydroidea › Drosophilidae › Drosophila › Sophophora

Protein attributes

Sequence length	508 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Evidence at transcript level.

General annotation (Comments)

Function	Mediates cell adhesion in a Ca2+-independent manner. It plays a role in axon outgrowth, guidance and fasciculation of the developing nervous system.
Subcellular location	Membrane; Single-pass type I membrane protein.
Tissue specificity	Expressed on different subsets of axon bundles (fascicles) in insect embryos.
Sequence similarities	Contains 2 Ig-like C2-type (immunoglobulin-like) domains. Contains 1 Ig-like V-type (immunoglobulin-like) domain.

Ontologies

Keywords
Biological process	Cell adhesion Differentiation Neurogenesis
Cellular component	Membrane
Coding sequence diversity	Alternative splicing
Domain	Immunoglobulin domain Repeat Signal Transmembrane
Molecular function	Developmental protein
PTM	Disulfide bond Glycoprotein Phosphoprotein Pyrrolidone carboxylic acid
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	axon guidance Ref.1 Inferred from expression pattern. Source: FlyBase axonal fasciculation Traceable author statement. Source: FlyBase homophilic cell adhesion Traceable author statement. Source: FlyBase olfactory learning Inferred from mutant phenotype. Source: FlyBase ovarian follicle cell development Traceable author statement. Source: FlyBase synaptic target attraction Traceable author statement. Source: FlyBase
Cellular component	basolateral plasma membrane Non-traceable author statement. Source: FlyBase integral to membrane Inferred from electronic annotation. Source: UniProtKB-KW lateral plasma membrane Traceable author statement. Source: FlyBase septate junction Traceable author statement. Source: FlyBase
Molecular function	protein binding Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Alternative products

This entry describes 2 isoforms produced by alternative splicing. [Align] [Select]

Isoform A (identifier: P15278-1)

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

Isoform B (identifier: P15278-2)

The sequence of this isoform differs from the canonical sequence as follows:
     391-401: SDTESADIKAT → KTNNSSMLNLY
     402-508: Missing.

Note: No experimental confirmation available.

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Signal peptide	1 – 20	20
	Chain	21 – 508	488	Fasciclin-3		PRO_0000014760
Regions
	Topological domain	21 – 346	326	Extracellular Potential
	Transmembrane	347 – 370	24	Potential
	Topological domain	371 – 508	138	Cytoplasmic Potential
	Domain	44 – 106	63	Ig-like V-type
	Domain	126 – 223	98	Ig-like C2-type 1
	Domain	236 – 310	75	Ig-like C2-type 2
Amino acid modifications
	Modified residue	21	1	Pyrrolidone carboxylic acid Probable
	Modified residue	382	1	Phosphoserine Potential
	Modified residue	459	1	Phosphoserine Potential
	Glycosylation	62	1	N-linked (GlcNAc...) Potential
	Glycosylation	160	1	N-linked (GlcNAc...) Potential
	Glycosylation	257	1	N-linked (GlcNAc...) Potential
	Glycosylation	300	1	N-linked (GlcNAc...) Potential
	Disulfide bond	150 ↔ 211		Potential
Natural variations
	Alternative sequence	391 – 401	11	SDTESADIKAT → KTNNSSMLNLY in isoform B.		VSP_007949
	Alternative sequence	402 – 508	107	Missing in isoform B.		VSP_007950

Sequences

	Sequence		Length	Mass (Da)
	Isoform A [UniParc]. Last modified April 1, 1990. Version 1. Checksum: 6E39EA0580697D4F	FASTA	508	55,884
	10 20 30 40 50 60 MSRIVFICLA AILTDALTWA QVNVEPNTAL LNEGDRTELL CRYGRSINYC RIEIPGEQKV 70 80 90 100 110 120 LNLSPEWSKT PGFTYFGAGL TAGQCGVSIE RVKASNNGQV KCSLGVEGEE LSGTIDLVVA 130 140 150 160 170 180 LRPQQPIIEL LSRPNREGYF NEGTEFRARC SVRDGRPPAN ISWYIDNMPA NKRTTPLEVM 190 200 210 220 230 240 SSTNDNVELS TSVQEIQWHL SPEDSNRKLV CRSHHQTDRE SVPPQEAAYI INVRYAPVHQ 250 260 270 280 290 300 PDAAVYGLYL EHTAIVNITI RASPQPKIEW TIDGAIVGQG RTDGRYSAYE PQYLGNDEYN 310 320 330 340 350 360 VTLAIAGLTL EDTTKIYNLR ASNELGLTDY QVRISSSSKP PSSSLDVAAI VGIVVAVAVL 370 380 390 400 410 420 VLVVLLIVFA RATGRWCFGG KSIKTPTNET SDTESADIKA TSTATATTTM GGVGVSAEEE 430 440 450 460 470 480 ETVNEQESPQ EQQQQQQKKA KRLPAFAAAI LRRFNEKDSR KYKDNQESLN IVEGSVQEIP 490 500 ATNNAIDGND NEPKAIVWQS TSPVWTFK
	Isoform B. Checksum: C2557D6AE0BB0DDC	FASTA	401	44,182
	10 20 30 40 50 60 MSRIVFICLA AILTDALTWA QVNVEPNTAL LNEGDRTELL CRYGRSINYC RIEIPGEQKV 70 80 90 100 110 120 LNLSPEWSKT PGFTYFGAGL TAGQCGVSIE RVKASNNGQV KCSLGVEGEE LSGTIDLVVA 130 140 150 160 170 180 LRPQQPIIEL LSRPNREGYF NEGTEFRARC SVRDGRPPAN ISWYIDNMPA NKRTTPLEVM 190 200 210 220 230 240 SSTNDNVELS TSVQEIQWHL SPEDSNRKLV CRSHHQTDRE SVPPQEAAYI INVRYAPVHQ 250 260 270 280 290 300 PDAAVYGLYL EHTAIVNITI RASPQPKIEW TIDGAIVGQG RTDGRYSAYE PQYLGNDEYN 310 320 330 340 350 360 VTLAIAGLTL EDTTKIYNLR ASNELGLTDY QVRISSSSKP PSSSLDVAAI VGIVVAVAVL 370 380 390 400 VLVVLLIVFA RATGRWCFGG KSIKTPTNET KTNNSSMLNL Y

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Fasciclin III: a novel homophilic adhesion molecule in Drosophila." Snow P.M., Bieber A.J., Goodman C.S. Cell 59:313-323(1989) [PubMed: 2509076] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM A).
[2]	"The genome sequence of Drosophila melanogaster." Adams M.D., Celniker S.E., Holt R.A., Evans C.A., Gocayne J.D., Amanatides P.G., Scherer S.E., Li P.W., Hoskins R.A., Galle R.F., George R.A., Lewis S.E., Richards S., Ashburner M., Henderson S.N., Sutton G.G., Wortman J.R., Yandell M.D. , Zhang Q., Chen L.X., Brandon R.C., Rogers Y.-H.C., Blazej R.G., Champe M., Pfeiffer B.D., Wan K.H., Doyle C., Baxter E.G., Helt G., Nelson C.R., Miklos G.L.G., Abril J.F., Agbayani A., An H.-J., Andrews-Pfannkoch C., Baldwin D., Ballew R.M., Basu A., Baxendale J., Bayraktaroglu L., Beasley E.M., Beeson K.Y., Benos P.V., Berman B.P., Bhandari D., Bolshakov S., Borkova D., Botchan M.R., Bouck J., Brokstein P., Brottier P., Burtis K.C., Busam D.A., Butler H., Cadieu E., Center A., Chandra I., Cherry J.M., Cawley S., Dahlke C., Davenport L.B., Davies P., de Pablos B., Delcher A., Deng Z., Mays A.D., Dew I., Dietz S.M., Dodson K., Doup L.E., Downes M., Dugan-Rocha S., Dunkov B.C., Dunn P., Durbin K.J., Evangelista C.C., Ferraz C., Ferriera S., Fleischmann W., Fosler C., Gabrielian A.E., Garg N.S., Gelbart W.M., Glasser K., Glodek A., Gong F., Gorrell J.H., Gu Z., Guan P., Harris M., Harris N.L., Harvey D.A., Heiman T.J., Hernandez J.R., Houck J., Hostin D., Houston K.A., Howland T.J., Wei M.-H., Ibegwam C., Jalali M., Kalush F., Karpen G.H., Ke Z., Kennison J.A., Ketchum K.A., Kimmel B.E., Kodira C.D., Kraft C.L., Kravitz S., Kulp D., Lai Z., Lasko P., Lei Y., Levitsky A.A., Li J.H., Li Z., Liang Y., Lin X., Liu X., Mattei B., McIntosh T.C., McLeod M.P., McPherson D., Merkulov G., Milshina N.V., Mobarry C., Morris J., Moshrefi A., Mount S.M., Moy M., Murphy B., Murphy L., Muzny D.M., Nelson D.L., Nelson D.R., Nelson K.A., Nixon K., Nusskern D.R., Pacleb J.M., Palazzolo M., Pittman G.S., Pan S., Pollard J., Puri V., Reese M.G., Reinert K., Remington K., Saunders R.D.C., Scheeler F., Shen H., Shue B.C., Siden-Kiamos I., Simpson M., Skupski M.P., Smith T.J., Spier E., Spradling A.C., Stapleton M., Strong R., Sun E., Svirskas R., Tector C., Turner R., Venter E., Wang A.H., Wang X., Wang Z.-Y., Wassarman D.A., Weinstock G.M., Weissenbach J., Williams S.M., Woodage T., Worley K.C., Wu D., Yang S., Yao Q.A., Ye J., Yeh R.-F., Zaveri J.S., Zhan M., Zhang G., Zhao Q., Zheng L., Zheng X.H., Zhong F.N., Zhong W., Zhou X., Zhu S.C., Zhu X., Smith H.O., Gibbs R.A., Myers E.W., Rubin G.M., Venter J.C. Science 287:2185-2195(2000) [PubMed: 10731132] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: Berkeley.
[3]	"Annotation of the Drosophila melanogaster euchromatic genome: a systematic review." Misra S., Crosby M.A., Mungall C.J., Matthews B.B., Campbell K.S., Hradecky P., Huang Y., Kaminker J.S., Millburn G.H., Prochnik S.E., Smith C.D., Tupy J.L., Whitfield E.J., Bayraktaroglu L., Berman B.P., Bettencourt B.R., Celniker S.E., de Grey A.D.N.J. , Drysdale R.A., Harris N.L., Richter J., Russo S., Schroeder A.J., Shu S.Q., Stapleton M., Yamada C., Ashburner M., Gelbart W.M., Rubin G.M., Lewis S.E. Genome Biol. 3:RESEARCH0083.1-RESEARCH0083.22(2002) [PubMed: 12537572] [Abstract] Cited for: GENOME REANNOTATION, ALTERNATIVE SPLICING.
[4]	"A Drosophila full-length cDNA resource." Stapleton M., Carlson J.W., Brokstein P., Yu C., Champe M., George R.A., Guarin H., Kronmiller B., Pacleb J.M., Park S., Wan K.H., Rubin G.M., Celniker S.E. Genome Biol. 3:RESEARCH0080.1-RESEARCH0080.8(2002) [PubMed: 12537569] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM B). Strain: Berkeley. Tissue: Embryo.

Cross-references

Sequence databases
EMBLGenBankDDBJ	M27813 mRNA. Translation: AAA28532.1. AE014134 Genomic DNA. Translation: AAF53665.2. AE014134 Genomic DNA. Translation: AAN11002.1. AY075516 mRNA. Translation: AAL68324.1.
PIR	A33378.
RefSeq	NP_724106.1. NP_724107.1.
UniGene	Dm.6733
3D structure databases
ModBase	Search...
Protein-protein interaction databases
DIP	DIP:21336N.
IntAct	P15278. 5 interactions.
Proteomic databases
PRIDE	P15278.
Genome annotation databases
Ensembl	FBgn0000636. Drosophila melanogaster. [Contig view]
GeneID	35097.
Organism-specific databases
FlyBase	FBgn0000636. Fas3.
Phylogenomic databases
HOGENOM	P15278.
OMA	P15278. DTESADI.
Enzyme and pathway databases
BioCyc	DMEL-XXX-02:DMEL-XXX-02-009341-MON.
Gene expression databases
ArrayExpress	P15278.
GermOnline	CG5803. Drosophila melanogaster.
Family and domain databases
InterPro	IPR013162. CD80_C2-set. IPR007110. Ig-like. [Graphical view]
Pfam	PF08205. C2-set_2. 1 hit. [Graphical view]
PROSITE	PS50835. IG_LIKE. 1 hit. [Graphical view]
ProtoNet	Search...
Other Resources
NextBio	791841.

Entry information

Entry name

FAS3_DROME

Accession

Primary (citable) accession number: P15278
Secondary accession number(s): Q8SXX9, Q9VJ89

Entry history

Integrated into UniProtKB/Swiss-Prot:	April 1, 1990
Last sequence update:	April 1, 1990
Last modified:	June 16, 2009
This is version 98 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

Drosophila annotation project