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Protein

AMP deaminase

Gene

AMD1

Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

AMP deaminase plays a critical role in energy metabolism.

Miscellaneous

Present with 3910 molecules/cell in log phase SD medium.1 Publication

Catalytic activityi

AMP + H2O = IMP + NH3.

Cofactori

Zn2+By similarityNote: Binds 1 zinc ion per subunit.By similarity

Pathwayi: IMP biosynthesis via salvage pathway

This protein is involved in step 1 of the subpathway that synthesizes IMP from AMP.
Proteins known to be involved in this subpathway in this organism are:
  1. AMP deaminase (AMD1)
This subpathway is part of the pathway IMP biosynthesis via salvage pathway, which is itself part of Purine metabolism.
View all proteins of this organism that are known to be involved in the subpathway that synthesizes IMP from AMP, the pathway IMP biosynthesis via salvage pathway and in Purine metabolism.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Metal bindingi362Zinc; catalyticBy similarity1
Metal bindingi364Zinc; catalyticBy similarity1
Binding sitei364SubstrateBy similarity1
Metal bindingi630Zinc; catalyticBy similarity1
Binding sitei633SubstrateBy similarity1
Active sitei652Proton acceptorPROSITE-ProRule annotation1
Metal bindingi707Zinc; catalyticBy similarity1

GO - Molecular functioni

  • AMP deaminase activity Source: SGD
  • metal ion binding Source: UniProtKB-KW

GO - Biological processi

  • guanine salvage Source: SGD
  • IMP salvage Source: UniProtKB-UniPathway
  • purine nucleotide metabolic process Source: SGD

Keywordsi

Molecular functionHydrolase
Biological processNucleotide metabolism
LigandMetal-binding, Zinc

Enzyme and pathway databases

BioCyciYEAST:YML035C-MONOMER
ReactomeiR-SCE-6798695 Neutrophil degranulation
R-SCE-74217 Purine salvage
UniPathwayiUPA00591; UER00663

Names & Taxonomyi

Protein namesi
Recommended name:
AMP deaminase (EC:3.5.4.6)
Alternative name(s):
Myoadenylate deaminase
Gene namesi
Name:AMD1
Synonyms:AMD
Ordered Locus Names:YML035C
OrganismiSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Taxonomic identifieri559292 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces
Proteomesi
  • UP000002311 Componenti: Chromosome XIII

Organism-specific databases

EuPathDBiFungiDB:YML035C
SGDiS000004498 AMD1

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00001944141 – 810AMP deaminaseAdd BLAST810

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei19PhosphoserineCombined sources1
Modified residuei58PhosphoserineCombined sources1
Modified residuei61PhosphoserineCombined sources1
Modified residuei138PhosphoserineCombined sources1

Keywords - PTMi

Phosphoprotein

Proteomic databases

MaxQBiP15274
PaxDbiP15274
PRIDEiP15274

PTM databases

iPTMnetiP15274

Interactioni

Subunit structurei

Homotetramer.

Binary interactionsi

WithEntry#Exp.IntActNotes
PRP40P332032EBI-2548,EBI-701

Protein-protein interaction databases

BioGridi35135, 208 interactors
DIPiDIP-1949N
IntActiP15274, 11 interactors
MINTiP15274
STRINGi4932.YML035C

Structurei

3D structure databases

ProteinModelPortaliP15274
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni433 – 438Substrate bindingBy similarity6
Regioni708 – 711Substrate bindingBy similarity4

Sequence similaritiesi

Phylogenomic databases

GeneTreeiENSGT00390000008190
HOGENOMiHOG000092200
InParanoidiP15274
KOiK01490
OMAiQTLMEVF
OrthoDBiEOG092C0YBC

Family and domain databases

CDDicd01319 AMPD, 1 hit
InterProiView protein in InterPro
IPR006650 A/AMP_deam_AS
IPR001365 A/AMP_deaminase_dom
IPR006329 AMPD
IPR032466 Metal_Hydrolase
PANTHERiPTHR11359 PTHR11359, 1 hit
PfamiView protein in Pfam
PF00962 A_deaminase, 1 hit
PIRSFiPIRSF001251 AMP_deaminase_met, 1 hit
SUPFAMiSSF51556 SSF51556, 1 hit
TIGRFAMsiTIGR01429 AMP_deaminase, 1 hit
PROSITEiView protein in PROSITE
PS00485 A_DEAMINASE, 1 hit

Sequencei

Sequence statusi: Complete.

P15274-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MDNQATQRLN DLSLEPAPSH DEQDGSGLVI DIDQRKIGDE QAGVVVDDET
60 70 80 90 100
PPLEQQDSHE SLAADSRNAN FSYHENQQLL ENGTKQLALD EHDSHSAILE
110 120 130 140 150
QPSHSTNCSS SNIAAMNKGH DSADHASQNS GGKPRTLSAS AQHILPETLK
160 170 180 190 200
SFAGAPVVNK QVRTSASYKM GMLADDASQQ FLDDPSSELI DLYSKVAECR
210 220 230 240 250
NLRAKYQTIS VQNDDQNPKN KPGWVVYPPP PKPSYNSDTK TVVPVTNKPD
260 270 280 290 300
AEVFDFTKCE IPGEDPDWEF TLNDDDSYVV HRSGKTDELI AQIPTLRDYY
310 320 330 340 350
LDLEKMISIS SDGPAKSFAY RRLQYLEARW NLYYLLNEYQ ETSVSKRNPH
360 370 380 390 400
RDFYNVRKVD THVHHSACMN QKHLLRFIKH KLRHSKDEKV IFRDGKLLTL
410 420 430 440 450
DEVFRSLHLT GYDLSIDTLD MHAHKDTFHR FDKFNLKYNP IGESRLREIF
460 470 480 490 500
LKTNNYIKGT YLADITKQVI FDLENSKYQN CEYRISVYGR SLDEWDKLAS
510 520 530 540 550
WVIDNKVISH NVRWLVQIPR LYDIYKKTGI VQSFQDICKN LFQPLFEVTK
560 570 580 590 600
NPQSHPKLHV FLQRVIGFDS VDDESKVDRR FHRKYPKPSL WEAPQNPPYS
610 620 630 640 650
YYLYYLYSNV ASLNQWRAKR GFNTLVLRPH CGEAGDPEHL VSAYLLAHGI
660 670 680 690 700
SHGILLRKVP FVQYLYYLDQ VGIAMSPLSN NALFLTYDKN PFPRYFKRGL
710 720 730 740 750
NVSLSTDDPL QFSYTREPLI EEYSVAAQIY KLSNVDMCEL ARNSVLQSGW
760 770 780 790 800
EAQIKKHWIG KDFDKSGVEG NDVVRTNVPD IRINYRYDTL STELELVNHF
810
ANFKRTIEEK
Length:810
Mass (Da):93,302
Last modified:February 1, 1996 - v2
Checksum:i7A6DCB43B9B45C93
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti568F → C in AAA34420 (PubMed:2690949).Curated1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
M30449 Genomic DNA Translation: AAA34420.1
Z46659 Genomic DNA Translation: CAA86620.1
BK006946 Genomic DNA Translation: DAA09864.1
PIRiS49744
RefSeqiNP_013677.1, NM_001182392.1

Genome annotation databases

EnsemblFungiiYML035C; YML035C; YML035C
GeneIDi854973
KEGGisce:YML035C

Similar proteinsi

Entry informationi

Entry nameiAMPD_YEAST
AccessioniPrimary (citable) accession number: P15274
Secondary accession number(s): D6VZE0
Entry historyiIntegrated into UniProtKB/Swiss-Prot: April 1, 1990
Last sequence update: February 1, 1996
Last modified: May 23, 2018
This is version 172 of the entry and version 2 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

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