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Protein

Tyrosine-protein phosphatase YopH

Gene

yopH

Organism
Yersinia enterocolitica
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Experimental evidence at protein leveli

Functioni

Essential virulence determinant. This protein is a protein tyrosine phosphatase. The essential function of YopH in Yersinia pathogenesis is host-protein dephosphorylation. It contributes to the ability of the bacteria to resist phagocytosis by peritoneal macrophages.1 Publication

Catalytic activityi

Protein tyrosine phosphate + H2O = protein tyrosine + phosphate.PROSITE-ProRule annotation1 Publication

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Active sitei403 – 4031Phosphocysteine intermediate

GO - Molecular functioni

  1. protein tyrosine phosphatase activity Source: UniProtKB-EC

GO - Biological processi

  1. pathogenesis Source: UniProtKB-KW
Complete GO annotation...

Keywords - Molecular functioni

Hydrolase, Protein phosphatase

Keywords - Biological processi

Virulence

Enzyme and pathway databases

BRENDAi3.1.3.48. 6741.
SABIO-RKP15273.

Names & Taxonomyi

Protein namesi
Recommended name:
Tyrosine-protein phosphatase YopH (EC:3.1.3.48)
Alternative name(s):
Virulence protein
Gene namesi
Name:yopH
Synonyms:yop51
Encoded oniPlasmid pYV0 Publication
OrganismiYersinia enterocolitica
Taxonomic identifieri630 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaEnterobacterialesEnterobacteriaceaeYersinia

Subcellular locationi

  1. Secreted

  2. Note: Secreted via type III secretion system.

GO - Cellular componenti

  1. extracellular region Source: UniProtKB-SubCell
Complete GO annotation...

Keywords - Cellular componenti

Secreted

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 468468Tyrosine-protein phosphatase YopHPRO_0000094861Add
BLAST

Expressioni

Inductioni

At 37 degrees Celsius in the absence of calcium.

Interactioni

Subunit structurei

Monomer.

Structurei

Secondary structure

1
468
Legend: HelixTurnBeta strand
Show more details
Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Beta strandi37 – 393Combined sources
Beta strandi42 – 465Combined sources
Helixi50 – 6213Combined sources
Helixi191 – 20818Combined sources
Beta strandi221 – 2244Combined sources
Helixi236 – 2383Combined sources
Beta strandi239 – 2413Combined sources
Beta strandi246 – 2516Combined sources
Beta strandi254 – 2596Combined sources
Helixi264 – 2663Combined sources
Helixi267 – 27610Combined sources
Beta strandi282 – 2843Combined sources
Helixi288 – 2925Combined sources
Helixi294 – 2963Combined sources
Beta strandi302 – 3043Combined sources
Beta strandi306 – 3083Combined sources
Beta strandi311 – 32414Combined sources
Beta strandi327 – 33812Combined sources
Beta strandi344 – 3518Combined sources
Beta strandi356 – 3583Combined sources
Helixi362 – 38524Combined sources
Helixi389 – 3924Combined sources
Beta strandi394 – 3974Combined sources
Beta strandi400 – 4023Combined sources
Beta strandi404 – 4085Combined sources
Helixi409 – 41911Combined sources
Helixi422 – 4243Combined sources
Helixi429 – 43911Combined sources
Helixi448 – 46114Combined sources

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
1LYVX-ray1.36A163-468[»]
1PA9X-ray2.00A185-468[»]
1XXPX-ray3.00A/B163-468[»]
1XXVX-ray2.50A/B163-468[»]
1YPTX-ray2.50A/B164-468[»]
1YTNX-ray2.40A164-468[»]
1YTSX-ray2.50A191-468[»]
1YTWX-ray2.40A164-468[»]
2I42X-ray2.20A164-468[»]
3BLTX-ray2.20A164-468[»]
3BLUX-ray2.00A164-468[»]
3BM8X-ray2.70A164-468[»]
3F99X-ray1.65A164-468[»]
3F9AX-ray1.69A164-468[»]
3F9BX-ray1.42A164-468[»]
3U96X-ray1.80A/B163-468[»]
4GF3X-ray1.90B21-63[»]
ProteinModelPortaliP15273.
SMRiP15273. Positions 1-129, 182-468.
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiP15273.

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini152 – 461310Tyrosine-protein phosphatasePROSITE-ProRule annotationAdd
BLAST

Sequence similaritiesi

Contains 1 tyrosine-protein phosphatase domain.PROSITE-ProRule annotation

Family and domain databases

Gene3Di3.30.1570.10. 1 hit.
3.90.190.10. 1 hit.
InterProiIPR029021. Prot-tyrosine_phosphatase-like.
IPR015103. ProtTyrPase_YopH_N.
IPR000387. Tyr/Dual-sp_Pase.
IPR016130. Tyr_Pase_AS.
IPR000242. Tyr_Pase_rcpt/non-rcpt.
IPR003546. Tyr_Pase_SptP/YopH.
[Graphical view]
PfamiPF00102. Y_phosphatase. 1 hit.
PF09013. YopH_N. 1 hit.
[Graphical view]
PRINTSiPR01371. BACYPHPHTASE.
PR00700. PRTYPHPHTASE.
SMARTiSM00194. PTPc. 1 hit.
[Graphical view]
SUPFAMiSSF52799. SSF52799. 1 hit.
SSF64449. SSF64449. 1 hit.
PROSITEiPS00383. TYR_PHOSPHATASE_1. 1 hit.
PS50056. TYR_PHOSPHATASE_2. 1 hit.
PS50055. TYR_PHOSPHATASE_PTP. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

P15273-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MNLSLSDLHR QVSRLVQQES GDCTGKLRGN VAANKETTFQ GLTIASGARE
60 70 80 90 100
SEKVFAQTVL SHVANIVLTQ EDTAKLLQST VKHNLNNYEL RSVGNGNSVL
110 120 130 140 150
VSLRSDQMTL QDAKVLLEAA LRQESGARGH VSSHSHSVLH APGTPVREGL
160 170 180 190 200
RSHLDPRTPP LPPRERPHTS GHHGAGEARA TAPSTVSPYG PEARAELSSR
210 220 230 240 250
LTTLRNTLAP ATNDPRYLQA CGGEKLNRFR DIQCCRQTAV RADLNANYIQ
260 270 280 290 300
VGNTRTIACQ YPLQSQLESH FRMLAENRTP VLAVLASSSE IANQRFGMPD
310 320 330 340 350
YFRQSGTYGS ITVESKMTQQ VGLGDGIMAD MYTLTIREAG QKTISVPVVH
360 370 380 390 400
VGNWPDQTAV SSEVTKALAS LVDQTAETKR NMYESKGSSA VADDSKLRPV
410 420 430 440 450
IHCRAGVGRT AQLIGAMCMN DSRNSQLSVE DMVSQMRVQR NGIMVQKDEQ
460
LDVLIKLAEG QGRPLLNS
Length:468
Mass (Da):50,939
Last modified:April 1, 1990 - v1
Checksum:iB207D0B1AC830AF9
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
M30457 Unassigned DNA. Translation: AAA19860.1.
PIRiA53889.
RefSeqiNP_052424.1. NC_002120.1.
WP_010891234.1. NZ_KN150737.1.

Genome annotation databases

GeneIDi1239155.
KEGGipg:1239155.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
M30457 Unassigned DNA. Translation: AAA19860.1.
PIRiA53889.
RefSeqiNP_052424.1. NC_002120.1.
WP_010891234.1. NZ_KN150737.1.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
1LYVX-ray1.36A163-468[»]
1PA9X-ray2.00A185-468[»]
1XXPX-ray3.00A/B163-468[»]
1XXVX-ray2.50A/B163-468[»]
1YPTX-ray2.50A/B164-468[»]
1YTNX-ray2.40A164-468[»]
1YTSX-ray2.50A191-468[»]
1YTWX-ray2.40A164-468[»]
2I42X-ray2.20A164-468[»]
3BLTX-ray2.20A164-468[»]
3BLUX-ray2.00A164-468[»]
3BM8X-ray2.70A164-468[»]
3F99X-ray1.65A164-468[»]
3F9AX-ray1.69A164-468[»]
3F9BX-ray1.42A164-468[»]
3U96X-ray1.80A/B163-468[»]
4GF3X-ray1.90B21-63[»]
ProteinModelPortaliP15273.
SMRiP15273. Positions 1-129, 182-468.
ModBaseiSearch...
MobiDBiSearch...

Chemistry

BindingDBiP15273.
ChEMBLiCHEMBL4404.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

GeneIDi1239155.
KEGGipg:1239155.

Enzyme and pathway databases

BRENDAi3.1.3.48. 6741.
SABIO-RKP15273.

Miscellaneous databases

EvolutionaryTraceiP15273.
PROiP15273.

Family and domain databases

Gene3Di3.30.1570.10. 1 hit.
3.90.190.10. 1 hit.
InterProiIPR029021. Prot-tyrosine_phosphatase-like.
IPR015103. ProtTyrPase_YopH_N.
IPR000387. Tyr/Dual-sp_Pase.
IPR016130. Tyr_Pase_AS.
IPR000242. Tyr_Pase_rcpt/non-rcpt.
IPR003546. Tyr_Pase_SptP/YopH.
[Graphical view]
PfamiPF00102. Y_phosphatase. 1 hit.
PF09013. YopH_N. 1 hit.
[Graphical view]
PRINTSiPR01371. BACYPHPHTASE.
PR00700. PRTYPHPHTASE.
SMARTiSM00194. PTPc. 1 hit.
[Graphical view]
SUPFAMiSSF52799. SSF52799. 1 hit.
SSF64449. SSF64449. 1 hit.
PROSITEiPS00383. TYR_PHOSPHATASE_1. 1 hit.
PS50056. TYR_PHOSPHATASE_2. 1 hit.
PS50055. TYR_PHOSPHATASE_PTP. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

  1. "Nucleotide sequence and transcription analysis of yop51 from Yersinia enterocolitica W22703."
    Michiels T., Cornelis G.
    Microb. Pathog. 5:449-459(1988) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
    Strain: W22703 / Serotype O:9 / Biotype 2.
  2. "Protein tyrosine phosphatase activity of an essential virulence determinant in Yersinia."
    Guan K.L., Dixon J.E.
    Science 249:553-556(1990) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION, CATALYTIC ACTIVITY.
  3. "Expression, purification, and physicochemical characterization of a recombinant Yersinia protein tyrosine phosphatase."
    Zhang Z.-Y., Clemens J.C., Schubert H.L., Stuckey J.A., Fischer M.W.F., Hume D.M., Saper M.A., Dixon J.E.
    J. Biol. Chem. 267:23759-23766(1992) [PubMed] [Europe PMC] [Abstract]
    Cited for: CHARACTERIZATION.
  4. "Crystal structure of Yersinia protein tyrosine phosphatase at 2.5 A and the complex with tungstate."
    Stuckey J.A., Schubert H.L., Fauman E.B., Zhang Z.-Y., Dixon J.E., Saper M.A.
    Nature 370:571-575(1994) [PubMed] [Europe PMC] [Abstract]
    Cited for: X-RAY CRYSTALLOGRAPHY (2.5 ANGSTROMS).
  5. "The X-ray crystal structures of Yersinia tyrosine phosphatase with bound tungstate and nitrate. Mechanistic implications."
    Fauman E.B., Yuvaniyama C., Schubert H.L., Stuckey J.A., Saper M.A.
    J. Biol. Chem. 271:18780-18788(1996) [PubMed] [Europe PMC] [Abstract]
    Cited for: X-RAY CRYSTALLOGRAPHY (2.4 ANGSTROMS) OF 186-468.

Entry informationi

Entry nameiYOPH_YEREN
AccessioniPrimary (citable) accession number: P15273
Entry historyi
Integrated into UniProtKB/Swiss-Prot: April 1, 1990
Last sequence update: April 1, 1990
Last modified: April 29, 2015
This is version 120 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Plasmid

Documents

  1. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.