Skip Header

You are using a version of Internet Explorer that may not display all features of this website. Please upgrade to a modern browser.
Contribute Send feedback
Read comments (?) or add your own

P15273 (YOPH_YEREN) Reviewed, UniProtKB/Swiss-Prot

Last modified July 9, 2014. Version 113. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Tyrosine-protein phosphatase YopH

EC=3.1.3.48
Alternative name(s):
Virulence protein
Gene names
Name:yopH
Synonyms:yop51
Encoded onPlasmid pYV
OrganismYersinia enterocolitica
Taxonomic identifier630 [NCBI]
Taxonomic lineageBacteriaProteobacteriaGammaproteobacteriaEnterobacterialesEnterobacteriaceaeYersinia

Protein attributes

Sequence length468 AA.
Sequence statusComplete.
Protein existenceEvidence at protein level

General annotation (Comments)

Function

Essential virulence determinant. This protein is a protein tyrosine phosphatase. The essential function of YopH in Yersinia pathogenesis is host-protein dephosphorylation. It contributes to the ability of the bacteria to resist phagocytosis by peritoneal macrophages. Ref.2

Catalytic activity

Protein tyrosine phosphate + H2O = protein tyrosine + phosphate. Ref.2

Subunit structure

Monomer.

Subcellular location

Secreted. Note: Secreted via type III secretion system.

Induction

At 37 degrees Celsius in the absence of calcium.

Sequence similarities

Belongs to the protein-tyrosine phosphatase family. Non-receptor class subfamily.

Contains 1 tyrosine-protein phosphatase domain.

Ontologies

Keywords
   Biological processVirulence
   Cellular componentSecreted
   Molecular functionHydrolase
Protein phosphatase
   Technical term3D-structure
Plasmid
Gene Ontology (GO)
   Biological_processpathogenesis

Inferred from electronic annotation. Source: UniProtKB-KW

   Cellular_componentextracellular region

Inferred from electronic annotation. Source: UniProtKB-SubCell

   Molecular_functionprotein tyrosine phosphatase activity

Inferred from electronic annotation. Source: UniProtKB-EC

Complete GO annotation...

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 468468Tyrosine-protein phosphatase YopH
PRO_0000094861

Regions

Domain152 – 461310Tyrosine-protein phosphatase

Sites

Active site4031Phosphocysteine intermediate

Secondary structure

........................................................ 468
Helix Strand Turn

Details...

Sequences

Sequence LengthMass (Da)Tools
P15273 [UniParc].

Last modified April 1, 1990. Version 1.
Checksum: B207D0B1AC830AF9

FASTA46850,939
        10         20         30         40         50         60 
MNLSLSDLHR QVSRLVQQES GDCTGKLRGN VAANKETTFQ GLTIASGARE SEKVFAQTVL 

        70         80         90        100        110        120 
SHVANIVLTQ EDTAKLLQST VKHNLNNYEL RSVGNGNSVL VSLRSDQMTL QDAKVLLEAA 

       130        140        150        160        170        180 
LRQESGARGH VSSHSHSVLH APGTPVREGL RSHLDPRTPP LPPRERPHTS GHHGAGEARA 

       190        200        210        220        230        240 
TAPSTVSPYG PEARAELSSR LTTLRNTLAP ATNDPRYLQA CGGEKLNRFR DIQCCRQTAV 

       250        260        270        280        290        300 
RADLNANYIQ VGNTRTIACQ YPLQSQLESH FRMLAENRTP VLAVLASSSE IANQRFGMPD 

       310        320        330        340        350        360 
YFRQSGTYGS ITVESKMTQQ VGLGDGIMAD MYTLTIREAG QKTISVPVVH VGNWPDQTAV 

       370        380        390        400        410        420 
SSEVTKALAS LVDQTAETKR NMYESKGSSA VADDSKLRPV IHCRAGVGRT AQLIGAMCMN 

       430        440        450        460 
DSRNSQLSVE DMVSQMRVQR NGIMVQKDEQ LDVLIKLAEG QGRPLLNS 

« Hide

References

[1]"Nucleotide sequence and transcription analysis of yop51 from Yersinia enterocolitica W22703."
Michiels T., Cornelis G.
Microb. Pathog. 5:449-459(1988) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
Strain: W22703 / Serotype O:9 / Biotype 2.
[2]"Protein tyrosine phosphatase activity of an essential virulence determinant in Yersinia."
Guan K.L., Dixon J.E.
Science 249:553-556(1990) [PubMed] [Europe PMC] [Abstract]
Cited for: FUNCTION, CATALYTIC ACTIVITY.
[3]"Expression, purification, and physicochemical characterization of a recombinant Yersinia protein tyrosine phosphatase."
Zhang Z.-Y., Clemens J.C., Schubert H.L., Stuckey J.A., Fischer M.W.F., Hume D.M., Saper M.A., Dixon J.E.
J. Biol. Chem. 267:23759-23766(1992) [PubMed] [Europe PMC] [Abstract]
Cited for: CHARACTERIZATION.
[4]"Crystal structure of Yersinia protein tyrosine phosphatase at 2.5 A and the complex with tungstate."
Stuckey J.A., Schubert H.L., Fauman E.B., Zhang Z.-Y., Dixon J.E., Saper M.A.
Nature 370:571-575(1994) [PubMed] [Europe PMC] [Abstract]
Cited for: X-RAY CRYSTALLOGRAPHY (2.5 ANGSTROMS).
[5]"The X-ray crystal structures of Yersinia tyrosine phosphatase with bound tungstate and nitrate. Mechanistic implications."
Fauman E.B., Yuvaniyama C., Schubert H.L., Stuckey J.A., Saper M.A.
J. Biol. Chem. 271:18780-18788(1996) [PubMed] [Europe PMC] [Abstract]
Cited for: X-RAY CRYSTALLOGRAPHY (2.4 ANGSTROMS) OF 186-468.
+Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
M30457 Unassigned DNA. Translation: AAA19860.1.
PIRA53889.
RefSeqNP_052424.1. NC_002120.1.

3D structure databases

PDBe
RCSB-PDB
PDBj
EntryMethodResolution (Å)ChainPositionsPDBsum
1LYVX-ray1.36A163-468[»]
1PA9X-ray2.00A185-468[»]
1XXPX-ray3.00A/B163-468[»]
1XXVX-ray2.50A/B163-468[»]
1YPTX-ray2.50A/B164-468[»]
1YTNX-ray2.40A164-468[»]
1YTSX-ray2.50A191-468[»]
1YTWX-ray2.40A164-468[»]
2I42X-ray2.20A164-468[»]
3BLTX-ray2.20A164-468[»]
3BLUX-ray2.00A164-468[»]
3BM8X-ray2.70A164-468[»]
3F99X-ray1.65A164-468[»]
3F9AX-ray1.69A164-468[»]
3F9BX-ray1.42A164-468[»]
3U96X-ray1.80A/B163-468[»]
4GF3X-ray1.90B21-63[»]
ProteinModelPortalP15273.
SMRP15273. Positions 1-129, 182-468.
ModBaseSearch...
MobiDBSearch...

Chemistry

BindingDBP15273.
ChEMBLCHEMBL4404.

Protein family/group databases

PptaseDBP3D0412160.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

GeneID1239155.

Enzyme and pathway databases

SABIO-RKP15273.

Family and domain databases

Gene3D3.30.1570.10. 1 hit.
3.90.190.10. 1 hit.
InterProIPR029021. Prot-tyrosine_phosphatase-like.
IPR015103. ProtTyrPase_YopH_N.
IPR000387. Tyr/Dual-sp_Pase.
IPR016130. Tyr_Pase_AS.
IPR000242. Tyr_Pase_rcpt/non-rcpt.
IPR003546. Tyr_Pase_SptP/YopH.
[Graphical view]
PfamPF00102. Y_phosphatase. 1 hit.
PF09013. YopH_N. 1 hit.
[Graphical view]
PRINTSPR01371. BACYPHPHTASE.
PR00700. PRTYPHPHTASE.
SMARTSM00194. PTPc. 1 hit.
[Graphical view]
SUPFAMSSF52799. SSF52799. 1 hit.
SSF64449. SSF64449. 1 hit.
PROSITEPS00383. TYR_PHOSPHATASE_1. 1 hit.
PS50056. TYR_PHOSPHATASE_2. 1 hit.
PS50055. TYR_PHOSPHATASE_PTP. 1 hit.
[Graphical view]
ProtoNetSearch...

Other

EvolutionaryTraceP15273.

Entry information

Entry nameYOPH_YEREN
AccessionPrimary (citable) accession number: P15273
Entry history
Integrated into UniProtKB/Swiss-Prot: April 1, 1990
Last sequence update: April 1, 1990
Last modified: July 9, 2014
This is version 113 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

PDB cross-references

Index of Protein Data Bank (PDB) cross-references