P15273 (YOPH_YEREN) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Tyrosine-protein phosphatase YopH
EC=3.1.3.48

Alternative name(s):
Virulence protein

Gene names

Name:	yopH
Synonyms:	yop51

Encoded on

Plasmid pYV

Organism

Yersinia enterocolitica

Taxonomic identifier

630 [NCBI]

Taxonomic lineage

cellular organisms › Bacteria › Proteobacteria › Gammaproteobacteria › Enterobacteriales › Enterobacteriaceae › Yersinia

Protein attributes

Sequence length	468 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	Essential virulence determinant. This protein is a protein tyrosine phosphatase. The essential function of YopH in Yersinia pathogenesis is host-protein dephosphorylation. It contributes to the ability of the bacteria to resist phagocytosis by peritoneal macrophages. Ref.2
Catalytic activity	Protein tyrosine phosphate + H2O = protein tyrosine + phosphate. Ref.2
Subunit structure	Monomer.
Subcellular location	Secreted. Note: Secreted via type III secretion system.
Induction	At 37 degrees Celsius in the absence of calcium.
Sequence similarities	Belongs to the protein-tyrosine phosphatase family. Non-receptor class subfamily. Contains 1 tyrosine-protein phosphatase domain.

Ontologies

Keywords
Biological process	Virulence
Cellular component	Secreted
Molecular function	Hydrolase Protein phosphatase
Technical term	3D-structure Plasmid
Gene Ontology (GO)
Biological_process	pathogenesis Inferred from electronic annotation. Source: UniProtKB-KW peptidyl-tyrosine dephosphorylation Inferred from electronic annotation. Source: GOC
Cellular_component	extracellular region Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular_function	protein tyrosine phosphatase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

Feature key

Position(s)

Length

Description

Graphical view

Feature identifier

Molecule processing

Chain

1 – 468

468

Tyrosine-protein phosphatase YopH

PRO_0000094861

Regions

Domain

152 – 461

310

Tyrosine-protein phosphatase

Sites

Active site

403

1

Phosphocysteine intermediate

Secondary structure

1

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468

Helix Strand Turn

Details...

Helix

191 – 208

18

Beta strand

221 – 224

4

Helix

236 – 238

3

Beta strand

239 – 241

3

Beta strand

246 – 251

6

Beta strand

254 – 259

6

Helix

264 – 266

3

Helix

267 – 276

10

Beta strand

282 – 284

3

Helix

288 – 292

5

Helix

294 – 296

3

Beta strand

302 – 304

3

Beta strand

306 – 308

3

Beta strand

311 – 324

14

Beta strand

327 – 338

12

Beta strand

344 – 351

8

Beta strand

356 – 358

3

Helix

362 – 385

24

Helix

389 – 392

4

Beta strand

394 – 397

4

Beta strand

400 – 402

3

Beta strand

404 – 408

5

Helix

409 – 419

11

Helix

422 – 424

3

Helix

429 – 439

11

Helix

448 – 461

14

Sequences

	Sequence		Length	Mass (Da)
	P15273 [UniParc]. Last modified April 1, 1990. Version 1. Checksum: B207D0B1AC830AF9	FASTA	468	50,939
	10 20 30 40 50 60 MNLSLSDLHR QVSRLVQQES GDCTGKLRGN VAANKETTFQ GLTIASGARE SEKVFAQTVL 70 80 90 100 110 120 SHVANIVLTQ EDTAKLLQST VKHNLNNYEL RSVGNGNSVL VSLRSDQMTL QDAKVLLEAA 130 140 150 160 170 180 LRQESGARGH VSSHSHSVLH APGTPVREGL RSHLDPRTPP LPPRERPHTS GHHGAGEARA 190 200 210 220 230 240 TAPSTVSPYG PEARAELSSR LTTLRNTLAP ATNDPRYLQA CGGEKLNRFR DIQCCRQTAV 250 260 270 280 290 300 RADLNANYIQ VGNTRTIACQ YPLQSQLESH FRMLAENRTP VLAVLASSSE IANQRFGMPD 310 320 330 340 350 360 YFRQSGTYGS ITVESKMTQQ VGLGDGIMAD MYTLTIREAG QKTISVPVVH VGNWPDQTAV 370 380 390 400 410 420 SSEVTKALAS LVDQTAETKR NMYESKGSSA VADDSKLRPV IHCRAGVGRT AQLIGAMCMN 430 440 450 460 DSRNSQLSVE DMVSQMRVQR NGIMVQKDEQ LDVLIKLAEG QGRPLLNS

References

[1]	"Nucleotide sequence and transcription analysis of yop51 from Yersinia enterocolitica W22703." Michiels T., Cornelis G. Microb. Pathog. 5:449-459(1988) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Strain: W22703 / Serotype O:9 / Biotype 2.
[2]	"Protein tyrosine phosphatase activity of an essential virulence determinant in Yersinia." Guan K.L., Dixon J.E. Science 249:553-556(1990) [PubMed] [Europe PMC] [Abstract] Cited for: FUNCTION, CATALYTIC ACTIVITY.
[3]	"Expression, purification, and physicochemical characterization of a recombinant Yersinia protein tyrosine phosphatase." Zhang Z.-Y., Clemens J.C., Schubert H.L., Stuckey J.A., Fischer M.W.F., Hume D.M., Saper M.A., Dixon J.E. J. Biol. Chem. 267:23759-23766(1992) [PubMed] [Europe PMC] [Abstract] Cited for: CHARACTERIZATION.
[4]	"Crystal structure of Yersinia protein tyrosine phosphatase at 2.5 A and the complex with tungstate." Stuckey J.A., Schubert H.L., Fauman E.B., Zhang Z.-Y., Dixon J.E., Saper M.A. Nature 370:571-575(1994) [PubMed] [Europe PMC] [Abstract] Cited for: X-RAY CRYSTALLOGRAPHY (2.5 ANGSTROMS).
[5]	"The X-ray crystal structures of Yersinia tyrosine phosphatase with bound tungstate and nitrate. Mechanistic implications." Fauman E.B., Yuvaniyama C., Schubert H.L., Stuckey J.A., Saper M.A. J. Biol. Chem. 271:18780-18788(1996) [PubMed] [Europe PMC] [Abstract] Cited for: X-RAY CRYSTALLOGRAPHY (2.4 ANGSTROMS) OF 186-468.
+	Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ

M30457 Unassigned DNA. Translation: AAA19860.1.

PIR

A53889.

RefSeq

NP_052424.1. NC_002120.1.

3D structure databases

PDBe
RCSB PDB
PDBj

Entry	Method	Resolution (Å)	Chain	Positions	PDBsum
1LYV	X-ray	1.36	A	164-468	[»]
1PA9	X-ray	2.00	A	185-468	[»]
1XXP	X-ray	3.00	A/B	163-468	[»]
1XXV	X-ray	2.50	A/B	163-468	[»]
1YPT	X-ray	2.50	A/B	164-468	[»]
1YTN	X-ray	2.40	A	164-468	[»]
1YTS	X-ray	2.50	A	191-468	[»]
1YTW	X-ray	2.40	A	164-468	[»]
2I42	X-ray	2.20	A	164-468	[»]
3BLT	X-ray	2.20	A	164-468	[»]
3BLU	X-ray	2.00	A	164-468	[»]
3BM8	X-ray	2.70	A	164-468	[»]
3F99	X-ray	1.65	A	164-468	[»]
3F9A	X-ray	1.69	A	164-468	[»]
3F9B	X-ray	1.42	A	164-468	[»]
3U96	X-ray	1.80	A/B	163-468	[»]
4GF3	X-ray	1.90	B	21-63	[»]

ProteinModelPortal

P15273.

SMR

P15273. Positions 1-129, 182-468.

ModBase

Search...

Protein family/group databases

PptaseDB

P3D0412160.

Protocols and materials databases

StructuralBiologyKnowledgebase

Search...

Genome annotation databases

GeneID

1239155.

Phylogenomic databases

ProtClustDB

CLSK862151.

Enzyme and pathway databases

SABIO-RK

P15273.

Family and domain databases

Gene3D

3.30.1570.10. 1 hit.

InterPro

IPR015103. ProtTyrPase_YopH_N.
IPR000387. Tyr/Dual-sp_Pase.
IPR016130. Tyr_Pase_AS.
IPR000242. Tyr_Pase_rcpt/non-rcpt.
IPR003546. Tyr_Pase_SptP/YopH.
[Graphical view]

Pfam

PF00102. Y_phosphatase. 1 hit.
PF09013. YopH_N. 1 hit.
[Graphical view]

PRINTS

PR01371. BACYPHPHTASE.
PR00700. PRTYPHPHTASE.

SMART

SM00194. PTPc. 1 hit.
[Graphical view]

SUPFAM

SSF64449. ProtTyrPase_YopH_N. 1 hit.

PROSITE

PS00383. TYR_PHOSPHATASE_1. 1 hit.
PS50056. TYR_PHOSPHATASE_2. 1 hit.
PS50055. TYR_PHOSPHATASE_PTP. 1 hit.
[Graphical view]

ProtoNet

Search...

Other

BindingDB

P15273.

ChEMBL

CHEMBL4404.

EvolutionaryTrace

P15273.

Entry information

Entry name

YOPH_YEREN

Accession

Primary (citable) accession number: P15273

Entry history

Integrated into UniProtKB/Swiss-Prot:	April 1, 1990
Last sequence update:	April 1, 1990
Last modified:	May 29, 2013
This is version 104 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program