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P15273

- YOPH_YEREN

UniProt

P15273 - YOPH_YEREN

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Protein
Tyrosine-protein phosphatase YopH
Gene
yopH, yop51
Organism
Yersinia enterocolitica
Status
Reviewed - Annotation score: 4 out of 5 - Experimental evidence at protein leveli

Functioni

Essential virulence determinant. This protein is a protein tyrosine phosphatase. The essential function of YopH in Yersinia pathogenesis is host-protein dephosphorylation. It contributes to the ability of the bacteria to resist phagocytosis by peritoneal macrophages.1 Publication

Catalytic activityi

Protein tyrosine phosphate + H2O = protein tyrosine + phosphate.1 Publication

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Active sitei403 – 4031Phosphocysteine intermediate

GO - Molecular functioni

  1. protein tyrosine phosphatase activity Source: UniProtKB-EC
Complete GO annotation...

GO - Biological processi

  1. pathogenesis Source: UniProtKB-KW
Complete GO annotation...

Keywords - Molecular functioni

Hydrolase, Protein phosphatase

Keywords - Biological processi

Virulence

Enzyme and pathway databases

SABIO-RKP15273.

Protein family/group databases

PptaseDBiP3D0412160.

Names & Taxonomyi

Protein namesi
Recommended name:
Tyrosine-protein phosphatase YopH (EC:3.1.3.48)
Alternative name(s):
Virulence protein
Gene namesi
Name:yopH
Synonyms:yop51
Encoded oniPlasmid pYV0 Publication
OrganismiYersinia enterocolitica
Taxonomic identifieri630 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaEnterobacterialesEnterobacteriaceaeYersinia

Subcellular locationi

Secreted
Note: Secreted via type III secretion system.

GO - Cellular componenti

  1. extracellular region Source: UniProtKB-SubCell
Complete GO annotation...

Keywords - Cellular componenti

Secreted

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 468468Tyrosine-protein phosphatase YopH
PRO_0000094861Add
BLAST

Expressioni

Inductioni

At 37 degrees Celsius in the absence of calcium.

Interactioni

Subunit structurei

Monomer.

Structurei

Secondary structure

Legend: HelixTurnBeta strand
Show more details
Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Beta strandi37 – 393
Beta strandi42 – 465
Helixi50 – 6213
Helixi191 – 20818
Beta strandi221 – 2244
Helixi236 – 2383
Beta strandi239 – 2413
Beta strandi246 – 2516
Beta strandi254 – 2596
Helixi264 – 2663
Helixi267 – 27610
Beta strandi282 – 2843
Helixi288 – 2925
Helixi294 – 2963
Beta strandi302 – 3043
Beta strandi306 – 3083
Beta strandi311 – 32414
Beta strandi327 – 33812
Beta strandi344 – 3518
Beta strandi356 – 3583
Helixi362 – 38524
Helixi389 – 3924
Beta strandi394 – 3974
Beta strandi400 – 4023
Beta strandi404 – 4085
Helixi409 – 41911
Helixi422 – 4243
Helixi429 – 43911
Helixi448 – 46114

3D structure databases

Select the link destinations:
PDBe
RCSB PDB
PDBj
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
1LYVX-ray1.36A163-468[»]
1PA9X-ray2.00A185-468[»]
1XXPX-ray3.00A/B163-468[»]
1XXVX-ray2.50A/B163-468[»]
1YPTX-ray2.50A/B164-468[»]
1YTNX-ray2.40A164-468[»]
1YTSX-ray2.50A191-468[»]
1YTWX-ray2.40A164-468[»]
2I42X-ray2.20A164-468[»]
3BLTX-ray2.20A164-468[»]
3BLUX-ray2.00A164-468[»]
3BM8X-ray2.70A164-468[»]
3F99X-ray1.65A164-468[»]
3F9AX-ray1.69A164-468[»]
3F9BX-ray1.42A164-468[»]
3U96X-ray1.80A/B163-468[»]
4GF3X-ray1.90B21-63[»]
ProteinModelPortaliP15273.
SMRiP15273. Positions 1-129, 182-468.

Miscellaneous databases

EvolutionaryTraceiP15273.

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini152 – 461310Tyrosine-protein phosphatase
Add
BLAST

Sequence similaritiesi

Family and domain databases

Gene3Di3.30.1570.10. 1 hit.
3.90.190.10. 1 hit.
InterProiIPR029021. Prot-tyrosine_phosphatase-like.
IPR015103. ProtTyrPase_YopH_N.
IPR000387. Tyr/Dual-sp_Pase.
IPR016130. Tyr_Pase_AS.
IPR000242. Tyr_Pase_rcpt/non-rcpt.
IPR003546. Tyr_Pase_SptP/YopH.
[Graphical view]
PfamiPF00102. Y_phosphatase. 1 hit.
PF09013. YopH_N. 1 hit.
[Graphical view]
PRINTSiPR01371. BACYPHPHTASE.
PR00700. PRTYPHPHTASE.
SMARTiSM00194. PTPc. 1 hit.
[Graphical view]
SUPFAMiSSF52799. SSF52799. 1 hit.
SSF64449. SSF64449. 1 hit.
PROSITEiPS00383. TYR_PHOSPHATASE_1. 1 hit.
PS50056. TYR_PHOSPHATASE_2. 1 hit.
PS50055. TYR_PHOSPHATASE_PTP. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

P15273-1 [UniParc]FASTAAdd to Basket

« Hide

MNLSLSDLHR QVSRLVQQES GDCTGKLRGN VAANKETTFQ GLTIASGARE    50
SEKVFAQTVL SHVANIVLTQ EDTAKLLQST VKHNLNNYEL RSVGNGNSVL 100
VSLRSDQMTL QDAKVLLEAA LRQESGARGH VSSHSHSVLH APGTPVREGL 150
RSHLDPRTPP LPPRERPHTS GHHGAGEARA TAPSTVSPYG PEARAELSSR 200
LTTLRNTLAP ATNDPRYLQA CGGEKLNRFR DIQCCRQTAV RADLNANYIQ 250
VGNTRTIACQ YPLQSQLESH FRMLAENRTP VLAVLASSSE IANQRFGMPD 300
YFRQSGTYGS ITVESKMTQQ VGLGDGIMAD MYTLTIREAG QKTISVPVVH 350
VGNWPDQTAV SSEVTKALAS LVDQTAETKR NMYESKGSSA VADDSKLRPV 400
IHCRAGVGRT AQLIGAMCMN DSRNSQLSVE DMVSQMRVQR NGIMVQKDEQ 450
LDVLIKLAEG QGRPLLNS 468
Length:468
Mass (Da):50,939
Last modified:April 1, 1990 - v1
Checksum:iB207D0B1AC830AF9
GO

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
M30457 Unassigned DNA. Translation: AAA19860.1.
PIRiA53889.
RefSeqiNP_052424.1. NC_002120.1.

Genome annotation databases

GeneIDi1239155.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
M30457 Unassigned DNA. Translation: AAA19860.1 .
PIRi A53889.
RefSeqi NP_052424.1. NC_002120.1.

3D structure databases

Select the link destinations:
PDBe
RCSB PDB
PDBj
Links Updated
Entry Method Resolution (Å) Chain Positions PDBsum
1LYV X-ray 1.36 A 163-468 [» ]
1PA9 X-ray 2.00 A 185-468 [» ]
1XXP X-ray 3.00 A/B 163-468 [» ]
1XXV X-ray 2.50 A/B 163-468 [» ]
1YPT X-ray 2.50 A/B 164-468 [» ]
1YTN X-ray 2.40 A 164-468 [» ]
1YTS X-ray 2.50 A 191-468 [» ]
1YTW X-ray 2.40 A 164-468 [» ]
2I42 X-ray 2.20 A 164-468 [» ]
3BLT X-ray 2.20 A 164-468 [» ]
3BLU X-ray 2.00 A 164-468 [» ]
3BM8 X-ray 2.70 A 164-468 [» ]
3F99 X-ray 1.65 A 164-468 [» ]
3F9A X-ray 1.69 A 164-468 [» ]
3F9B X-ray 1.42 A 164-468 [» ]
3U96 X-ray 1.80 A/B 163-468 [» ]
4GF3 X-ray 1.90 B 21-63 [» ]
ProteinModelPortali P15273.
SMRi P15273. Positions 1-129, 182-468.
ModBasei Search...

Chemistry

BindingDBi P15273.
ChEMBLi CHEMBL4404.

Protein family/group databases

PptaseDBi P3D0412160.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

GeneIDi 1239155.

Enzyme and pathway databases

SABIO-RK P15273.

Miscellaneous databases

EvolutionaryTracei P15273.

Family and domain databases

Gene3Di 3.30.1570.10. 1 hit.
3.90.190.10. 1 hit.
InterProi IPR029021. Prot-tyrosine_phosphatase-like.
IPR015103. ProtTyrPase_YopH_N.
IPR000387. Tyr/Dual-sp_Pase.
IPR016130. Tyr_Pase_AS.
IPR000242. Tyr_Pase_rcpt/non-rcpt.
IPR003546. Tyr_Pase_SptP/YopH.
[Graphical view ]
Pfami PF00102. Y_phosphatase. 1 hit.
PF09013. YopH_N. 1 hit.
[Graphical view ]
PRINTSi PR01371. BACYPHPHTASE.
PR00700. PRTYPHPHTASE.
SMARTi SM00194. PTPc. 1 hit.
[Graphical view ]
SUPFAMi SSF52799. SSF52799. 1 hit.
SSF64449. SSF64449. 1 hit.
PROSITEi PS00383. TYR_PHOSPHATASE_1. 1 hit.
PS50056. TYR_PHOSPHATASE_2. 1 hit.
PS50055. TYR_PHOSPHATASE_PTP. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

  1. "Nucleotide sequence and transcription analysis of yop51 from Yersinia enterocolitica W22703."
    Michiels T., Cornelis G.
    Microb. Pathog. 5:449-459(1988) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
    Strain: W22703 / Serotype O:9 / Biotype 2.
  2. "Protein tyrosine phosphatase activity of an essential virulence determinant in Yersinia."
    Guan K.L., Dixon J.E.
    Science 249:553-556(1990) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION, CATALYTIC ACTIVITY.
  3. "Expression, purification, and physicochemical characterization of a recombinant Yersinia protein tyrosine phosphatase."
    Zhang Z.-Y., Clemens J.C., Schubert H.L., Stuckey J.A., Fischer M.W.F., Hume D.M., Saper M.A., Dixon J.E.
    J. Biol. Chem. 267:23759-23766(1992) [PubMed] [Europe PMC] [Abstract]
    Cited for: CHARACTERIZATION.
  4. "Crystal structure of Yersinia protein tyrosine phosphatase at 2.5 A and the complex with tungstate."
    Stuckey J.A., Schubert H.L., Fauman E.B., Zhang Z.-Y., Dixon J.E., Saper M.A.
    Nature 370:571-575(1994) [PubMed] [Europe PMC] [Abstract]
    Cited for: X-RAY CRYSTALLOGRAPHY (2.5 ANGSTROMS).
  5. "The X-ray crystal structures of Yersinia tyrosine phosphatase with bound tungstate and nitrate. Mechanistic implications."
    Fauman E.B., Yuvaniyama C., Schubert H.L., Stuckey J.A., Saper M.A.
    J. Biol. Chem. 271:18780-18788(1996) [PubMed] [Europe PMC] [Abstract]
    Cited for: X-RAY CRYSTALLOGRAPHY (2.4 ANGSTROMS) OF 186-468.

Entry informationi

Entry nameiYOPH_YEREN
AccessioniPrimary (citable) accession number: P15273
Entry historyi
Integrated into UniProtKB/Swiss-Prot: April 1, 1990
Last sequence update: April 1, 1990
Last modified: July 9, 2014
This is version 113 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Plasmid

Documents

  1. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

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