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P15273

- YOPH_YEREN

UniProt

P15273 - YOPH_YEREN

Protein

Tyrosine-protein phosphatase YopH

Gene

yopH

Organism
Yersinia enterocolitica
Status
Reviewed - Annotation score: 4 out of 5- Experimental evidence at protein leveli
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    • History
      Entry version 114 (01 Oct 2014)
      Sequence version 1 (01 Apr 1990)
      Previous versions | rss
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    Functioni

    Essential virulence determinant. This protein is a protein tyrosine phosphatase. The essential function of YopH in Yersinia pathogenesis is host-protein dephosphorylation. It contributes to the ability of the bacteria to resist phagocytosis by peritoneal macrophages.1 Publication

    Catalytic activityi

    Protein tyrosine phosphate + H2O = protein tyrosine + phosphate.1 PublicationPROSITE-ProRule annotation

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Active sitei403 – 4031Phosphocysteine intermediate

    GO - Molecular functioni

    1. protein tyrosine phosphatase activity Source: UniProtKB-EC

    GO - Biological processi

    1. pathogenesis Source: UniProtKB-KW

    Keywords - Molecular functioni

    Hydrolase, Protein phosphatase

    Keywords - Biological processi

    Virulence

    Enzyme and pathway databases

    SABIO-RKP15273.

    Protein family/group databases

    PptaseDBiP3D0412160.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Tyrosine-protein phosphatase YopH (EC:3.1.3.48)
    Alternative name(s):
    Virulence protein
    Gene namesi
    Name:yopH
    Synonyms:yop51
    Encoded oniPlasmid pYV0 Publication
    OrganismiYersinia enterocolitica
    Taxonomic identifieri630 [NCBI]
    Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaEnterobacterialesEnterobacteriaceaeYersinia

    Subcellular locationi

    Secreted
    Note: Secreted via type III secretion system.

    GO - Cellular componenti

    1. extracellular region Source: UniProtKB-SubCell

    Keywords - Cellular componenti

    Secreted

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 468468Tyrosine-protein phosphatase YopHPRO_0000094861Add
    BLAST

    Expressioni

    Inductioni

    At 37 degrees Celsius in the absence of calcium.

    Interactioni

    Subunit structurei

    Monomer.

    Structurei

    Secondary structure

    1
    468
    Legend: HelixTurnBeta strand
    Show more details
    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Beta strandi37 – 393
    Beta strandi42 – 465
    Helixi50 – 6213
    Helixi191 – 20818
    Beta strandi221 – 2244
    Helixi236 – 2383
    Beta strandi239 – 2413
    Beta strandi246 – 2516
    Beta strandi254 – 2596
    Helixi264 – 2663
    Helixi267 – 27610
    Beta strandi282 – 2843
    Helixi288 – 2925
    Helixi294 – 2963
    Beta strandi302 – 3043
    Beta strandi306 – 3083
    Beta strandi311 – 32414
    Beta strandi327 – 33812
    Beta strandi344 – 3518
    Beta strandi356 – 3583
    Helixi362 – 38524
    Helixi389 – 3924
    Beta strandi394 – 3974
    Beta strandi400 – 4023
    Beta strandi404 – 4085
    Helixi409 – 41911
    Helixi422 – 4243
    Helixi429 – 43911
    Helixi448 – 46114

    3D structure databases

    Select the link destinations:
    PDBe
    RCSB PDB
    PDBj
    Links Updated
    EntryMethodResolution (Å)ChainPositionsPDBsum
    1LYVX-ray1.36A163-468[»]
    1PA9X-ray2.00A185-468[»]
    1XXPX-ray3.00A/B163-468[»]
    1XXVX-ray2.50A/B163-468[»]
    1YPTX-ray2.50A/B164-468[»]
    1YTNX-ray2.40A164-468[»]
    1YTSX-ray2.50A191-468[»]
    1YTWX-ray2.40A164-468[»]
    2I42X-ray2.20A164-468[»]
    3BLTX-ray2.20A164-468[»]
    3BLUX-ray2.00A164-468[»]
    3BM8X-ray2.70A164-468[»]
    3F99X-ray1.65A164-468[»]
    3F9AX-ray1.69A164-468[»]
    3F9BX-ray1.42A164-468[»]
    3U96X-ray1.80A/B163-468[»]
    4GF3X-ray1.90B21-63[»]
    ProteinModelPortaliP15273.
    SMRiP15273. Positions 1-129, 182-468.
    ModBaseiSearch...
    MobiDBiSearch...

    Miscellaneous databases

    EvolutionaryTraceiP15273.

    Family & Domainsi

    Domains and Repeats

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Domaini152 – 461310Tyrosine-protein phosphatasePROSITE-ProRule annotationAdd
    BLAST

    Sequence similaritiesi

    Contains 1 tyrosine-protein phosphatase domain.PROSITE-ProRule annotation

    Family and domain databases

    Gene3Di3.30.1570.10. 1 hit.
    3.90.190.10. 1 hit.
    InterProiIPR029021. Prot-tyrosine_phosphatase-like.
    IPR015103. ProtTyrPase_YopH_N.
    IPR000387. Tyr/Dual-sp_Pase.
    IPR016130. Tyr_Pase_AS.
    IPR000242. Tyr_Pase_rcpt/non-rcpt.
    IPR003546. Tyr_Pase_SptP/YopH.
    [Graphical view]
    PfamiPF00102. Y_phosphatase. 1 hit.
    PF09013. YopH_N. 1 hit.
    [Graphical view]
    PRINTSiPR01371. BACYPHPHTASE.
    PR00700. PRTYPHPHTASE.
    SMARTiSM00194. PTPc. 1 hit.
    [Graphical view]
    SUPFAMiSSF52799. SSF52799. 1 hit.
    SSF64449. SSF64449. 1 hit.
    PROSITEiPS00383. TYR_PHOSPHATASE_1. 1 hit.
    PS50056. TYR_PHOSPHATASE_2. 1 hit.
    PS50055. TYR_PHOSPHATASE_PTP. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    P15273-1 [UniParc]FASTAAdd to Basket

    « Hide

    MNLSLSDLHR QVSRLVQQES GDCTGKLRGN VAANKETTFQ GLTIASGARE    50
    SEKVFAQTVL SHVANIVLTQ EDTAKLLQST VKHNLNNYEL RSVGNGNSVL 100
    VSLRSDQMTL QDAKVLLEAA LRQESGARGH VSSHSHSVLH APGTPVREGL 150
    RSHLDPRTPP LPPRERPHTS GHHGAGEARA TAPSTVSPYG PEARAELSSR 200
    LTTLRNTLAP ATNDPRYLQA CGGEKLNRFR DIQCCRQTAV RADLNANYIQ 250
    VGNTRTIACQ YPLQSQLESH FRMLAENRTP VLAVLASSSE IANQRFGMPD 300
    YFRQSGTYGS ITVESKMTQQ VGLGDGIMAD MYTLTIREAG QKTISVPVVH 350
    VGNWPDQTAV SSEVTKALAS LVDQTAETKR NMYESKGSSA VADDSKLRPV 400
    IHCRAGVGRT AQLIGAMCMN DSRNSQLSVE DMVSQMRVQR NGIMVQKDEQ 450
    LDVLIKLAEG QGRPLLNS 468
    Length:468
    Mass (Da):50,939
    Last modified:April 1, 1990 - v1
    Checksum:iB207D0B1AC830AF9
    GO

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    M30457 Unassigned DNA. Translation: AAA19860.1.
    PIRiA53889.
    RefSeqiNP_052424.1. NC_002120.1.

    Genome annotation databases

    GeneIDi1239155.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    M30457 Unassigned DNA. Translation: AAA19860.1 .
    PIRi A53889.
    RefSeqi NP_052424.1. NC_002120.1.

    3D structure databases

    Select the link destinations:
    PDBe
    RCSB PDB
    PDBj
    Links Updated
    Entry Method Resolution (Å) Chain Positions PDBsum
    1LYV X-ray 1.36 A 163-468 [» ]
    1PA9 X-ray 2.00 A 185-468 [» ]
    1XXP X-ray 3.00 A/B 163-468 [» ]
    1XXV X-ray 2.50 A/B 163-468 [» ]
    1YPT X-ray 2.50 A/B 164-468 [» ]
    1YTN X-ray 2.40 A 164-468 [» ]
    1YTS X-ray 2.50 A 191-468 [» ]
    1YTW X-ray 2.40 A 164-468 [» ]
    2I42 X-ray 2.20 A 164-468 [» ]
    3BLT X-ray 2.20 A 164-468 [» ]
    3BLU X-ray 2.00 A 164-468 [» ]
    3BM8 X-ray 2.70 A 164-468 [» ]
    3F99 X-ray 1.65 A 164-468 [» ]
    3F9A X-ray 1.69 A 164-468 [» ]
    3F9B X-ray 1.42 A 164-468 [» ]
    3U96 X-ray 1.80 A/B 163-468 [» ]
    4GF3 X-ray 1.90 B 21-63 [» ]
    ProteinModelPortali P15273.
    SMRi P15273. Positions 1-129, 182-468.
    ModBasei Search...
    MobiDBi Search...

    Chemistry

    BindingDBi P15273.
    ChEMBLi CHEMBL4404.

    Protein family/group databases

    PptaseDBi P3D0412160.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    GeneIDi 1239155.

    Enzyme and pathway databases

    SABIO-RK P15273.

    Miscellaneous databases

    EvolutionaryTracei P15273.

    Family and domain databases

    Gene3Di 3.30.1570.10. 1 hit.
    3.90.190.10. 1 hit.
    InterProi IPR029021. Prot-tyrosine_phosphatase-like.
    IPR015103. ProtTyrPase_YopH_N.
    IPR000387. Tyr/Dual-sp_Pase.
    IPR016130. Tyr_Pase_AS.
    IPR000242. Tyr_Pase_rcpt/non-rcpt.
    IPR003546. Tyr_Pase_SptP/YopH.
    [Graphical view ]
    Pfami PF00102. Y_phosphatase. 1 hit.
    PF09013. YopH_N. 1 hit.
    [Graphical view ]
    PRINTSi PR01371. BACYPHPHTASE.
    PR00700. PRTYPHPHTASE.
    SMARTi SM00194. PTPc. 1 hit.
    [Graphical view ]
    SUPFAMi SSF52799. SSF52799. 1 hit.
    SSF64449. SSF64449. 1 hit.
    PROSITEi PS00383. TYR_PHOSPHATASE_1. 1 hit.
    PS50056. TYR_PHOSPHATASE_2. 1 hit.
    PS50055. TYR_PHOSPHATASE_PTP. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "Nucleotide sequence and transcription analysis of yop51 from Yersinia enterocolitica W22703."
      Michiels T., Cornelis G.
      Microb. Pathog. 5:449-459(1988) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
      Strain: W22703 / Serotype O:9 / Biotype 2.
    2. "Protein tyrosine phosphatase activity of an essential virulence determinant in Yersinia."
      Guan K.L., Dixon J.E.
      Science 249:553-556(1990) [PubMed] [Europe PMC] [Abstract]
      Cited for: FUNCTION, CATALYTIC ACTIVITY.
    3. "Expression, purification, and physicochemical characterization of a recombinant Yersinia protein tyrosine phosphatase."
      Zhang Z.-Y., Clemens J.C., Schubert H.L., Stuckey J.A., Fischer M.W.F., Hume D.M., Saper M.A., Dixon J.E.
      J. Biol. Chem. 267:23759-23766(1992) [PubMed] [Europe PMC] [Abstract]
      Cited for: CHARACTERIZATION.
    4. "Crystal structure of Yersinia protein tyrosine phosphatase at 2.5 A and the complex with tungstate."
      Stuckey J.A., Schubert H.L., Fauman E.B., Zhang Z.-Y., Dixon J.E., Saper M.A.
      Nature 370:571-575(1994) [PubMed] [Europe PMC] [Abstract]
      Cited for: X-RAY CRYSTALLOGRAPHY (2.5 ANGSTROMS).
    5. "The X-ray crystal structures of Yersinia tyrosine phosphatase with bound tungstate and nitrate. Mechanistic implications."
      Fauman E.B., Yuvaniyama C., Schubert H.L., Stuckey J.A., Saper M.A.
      J. Biol. Chem. 271:18780-18788(1996) [PubMed] [Europe PMC] [Abstract]
      Cited for: X-RAY CRYSTALLOGRAPHY (2.4 ANGSTROMS) OF 186-468.

    Entry informationi

    Entry nameiYOPH_YEREN
    AccessioniPrimary (citable) accession number: P15273
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: April 1, 1990
    Last sequence update: April 1, 1990
    Last modified: October 1, 2014
    This is version 114 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programProkaryotic Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    3D-structure, Plasmid

    Documents

    1. PDB cross-references
      Index of Protein Data Bank (PDB) cross-references
    2. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3