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Protein

Hepatocyte nuclear factor 1-alpha

Gene

Hnf1a

Organism
Rattus norvegicus (Rat)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Transcriptional activator that regulates the tissue specific expression of multiple genes, especially in pancreatic islet cells and in liver. Required for the expression of several liver specific genes. Binds to the inverted palindrome 5'-GTTAATNATTAAC-3'.

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
DNA bindingi199 – 27981Homeobox; HNF1-typePROSITE-ProRule annotationAdd
BLAST

GO - Molecular functioni

  • DNA binding Source: MGI
  • double-stranded DNA binding Source: RGD
  • RNA polymerase II core promoter sequence-specific DNA binding Source: Ensembl
  • RNA polymerase II regulatory region sequence-specific DNA binding Source: GO_Central
  • sequence-specific DNA binding Source: RGD
  • transcriptional activator activity, RNA polymerase II core promoter proximal region sequence-specific binding Source: BHF-UCL
  • transcription cofactor binding Source: RGD
  • transcription factor activity, sequence-specific DNA binding Source: MGI
  • transcription factor binding Source: RGD
  • transcription regulatory region DNA binding Source: UniProtKB

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Activator

Keywords - Biological processi

Transcription, Transcription regulation

Keywords - Ligandi

DNA-binding

Names & Taxonomyi

Protein namesi
Recommended name:
Hepatocyte nuclear factor 1-alpha
Short name:
HNF-1-alpha
Short name:
HNF-1A
Alternative name(s):
Liver-specific transcription factor LF-B1
Short name:
LFB1
Transcription factor 1
Short name:
TCF-1
Gene namesi
Name:Hnf1a
Synonyms:Hnf-1, Hnf-1a, Tcf1
OrganismiRattus norvegicus (Rat)
Taxonomic identifieri10116 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeRattus
Proteomesi
  • UP000002494 Componenti: Chromosome 12

Organism-specific databases

RGDi3828. Hnf1a.

Subcellular locationi

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Nucleus

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 628628Hepatocyte nuclear factor 1-alphaPRO_0000049117Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei70 – 701PhosphoserineBy similarity
Modified residuei74 – 741PhosphothreonineCombined sources
Modified residuei93 – 931PhosphoserineBy similarity
Modified residuei247 – 2471PhosphoserineCombined sources
Modified residuei313 – 3131PhosphoserineBy similarity

Keywords - PTMi

Phosphoprotein

Proteomic databases

PaxDbiP15257.
PRIDEiP15257.

PTM databases

iPTMnetiP15257.
PhosphoSiteiP15257.

Expressioni

Tissue specificityi

Liver.

Gene expression databases

BgeeiENSRNOG00000001183.
GenevisibleiP15257. RN.

Interactioni

Subunit structurei

Binds DNA as a dimer. Interacts with PCBD1. Heterotetramer with PCBD1; formed by a dimer of dimers (By similarity).By similarity

GO - Molecular functioni

  • transcription cofactor binding Source: RGD
  • transcription factor binding Source: RGD

Protein-protein interaction databases

BioGridi246939. 1 interaction.
STRINGi10116.ENSRNOP00000001565.

Structurei

Secondary structure

1
628
Legend: HelixTurnBeta strand
Show more details
Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Helixi208 – 21811Combined sources
Helixi226 – 24116Combined sources
Turni242 – 2454Combined sources
Turni251 – 2544Combined sources
Helixi255 – 2573Combined sources
Helixi261 – 27313Combined sources

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
1LFBX-ray2.80A195-286[»]
2LFBNMR-A195-286[»]
ProteinModelPortaliP15257.
SMRiP15257. Positions 1-31, 85-286.
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiP15257.

Family & Domainsi

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni1 – 3131DimerizationAdd
BLAST
Regioni130 – 1323Interaction with DNABy similarity
Regioni143 – 1497Interaction with DNABy similarity
Regioni155 – 1584Interaction with DNABy similarity
Regioni203 – 2064Interaction with DNABy similarity
Regioni263 – 2653Interaction with DNABy similarity
Regioni270 – 2734Interaction with DNABy similarity

Motif

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Motifi197 – 2059Nuclear localization signalSequence analysis

Compositional bias

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Compositional biasi71 – 8010Asp/Glu-rich (acidic; potential involvement with transcription)

Sequence similaritiesi

Belongs to the HNF1 homeobox family.Curated
Contains 1 homeobox DNA-binding domain.PROSITE-ProRule annotation

Keywords - Domaini

Homeobox

Phylogenomic databases

eggNOGiENOG410IFA0. Eukaryota.
ENOG410ZZZ0. LUCA.
GeneTreeiENSGT00730000110937.
HOGENOMiHOG000015305.
HOVERGENiHBG005980.
InParanoidiP15257.
KOiK08036.
OMAiQSHVAQS.
OrthoDBiEOG091G052F.
PhylomeDBiP15257.
TreeFamiTF320327.

Family and domain databases

Gene3Di1.10.10.60. 1 hit.
1.10.260.40. 1 hit.
InterProiIPR006899. HNF-1_N.
IPR023219. HNF1_dimer_dom.
IPR006898. HNF1a_C.
IPR006897. HNF1b_C.
IPR001356. Homeobox_dom.
IPR009057. Homeodomain-like.
IPR010982. Lambda_DNA-bd_dom.
[Graphical view]
PfamiPF04814. HNF-1_N. 1 hit.
PF04813. HNF-1A_C. 1 hit.
PF04812. HNF-1B_C. 1 hit.
PF00046. Homeobox. 1 hit.
[Graphical view]
SMARTiSM00389. HOX. 1 hit.
[Graphical view]
SUPFAMiSSF100957. SSF100957. 1 hit.
SSF46689. SSF46689. 1 hit.
SSF47413. SSF47413. 1 hit.
PROSITEiPS00027. HOMEOBOX_1. 1 hit.
PS50071. HOMEOBOX_2. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

P15257-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MVSKLSQLQT ELLAALLESG LSKEALIQAL GEPGPYLMVG DGPLDKGESC
60 70 80 90 100
GGTRGDLTEL PNGLGETRGS EDDTDDDGED FAPPILKELE NLSPEEAAHQ
110 120 130 140 150
KAVVESLLQE DPWRVAKMVK SYLQQHNIPQ REVVDTTGLN QSHLSQHLNK
160 170 180 190 200
GTPMKTQKRA ALYTWYVRKQ REVAQQFTHA GQGGLIEEPT GDELPTKKGR
210 220 230 240 250
RNRFKWGPAS QQILFQAYER QKNPSKEERE TLVEECNRAE CIQRGVSPSQ
260 270 280 290 300
AQGLGSNLVT EVRVYNWFAN RRKEEAFRHK LAMDTYNGPP PGPGPGPALP
310 320 330 340 350
AHSSPGLPTT TLSPSKVHGV RYGQSATSEA AEVPSSSGGP LVTVSAALHQ
360 370 380 390 400
VSPTGLEPSS LLSTEAKLVS ATGGPLPPVS TLTALHSLEQ TSPGLNQQPQ
410 420 430 440 450
NLIMASLPGV MTIGPGEPAS LGPTFTNTGA STLVIGLAST QAQSVPVINS
460 470 480 490 500
MGSSLTTLQP VQFSQPLHPS YQQPLMPPVQ SHVAQSPFMA TMAQLQSPHA
510 520 530 540 550
LYSHKPEVAQ YTHTSLLPQT MLITDTNLST LASLTPTKQV FTSDTEASSE
560 570 580 590 600
PGLHEPSSPA TTIHIPSQDP SNIQHLQPAH RLSTSPTVSS SSLVLYQSSD
610 620
SNGHSHLLPS NHGVIETFIS TQMASSSQ
Length:628
Mass (Da):67,213
Last modified:April 1, 1990 - v1
Checksum:i8D28099308C86A52
GO

Sequence cautioni

The sequence CAA37387 differs from that shown. Reason: Erroneous initiation. Curated

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
J03170 mRNA. Translation: AAA41524.1.
X54423 mRNA. Translation: CAA38295.1.
X67649 Genomic DNA. Translation: CAA47891.1.
X53297 mRNA. Translation: CAA37387.1. Different initiation.
PIRiA33333.
S25485.
RefSeqiNP_036801.1. NM_012669.1.
UniGeneiRn.9660.

Genome annotation databases

EnsembliENSRNOT00000001565; ENSRNOP00000001565; ENSRNOG00000001183.
GeneIDi24817.
KEGGirno:24817.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
J03170 mRNA. Translation: AAA41524.1.
X54423 mRNA. Translation: CAA38295.1.
X67649 Genomic DNA. Translation: CAA47891.1.
X53297 mRNA. Translation: CAA37387.1. Different initiation.
PIRiA33333.
S25485.
RefSeqiNP_036801.1. NM_012669.1.
UniGeneiRn.9660.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
1LFBX-ray2.80A195-286[»]
2LFBNMR-A195-286[»]
ProteinModelPortaliP15257.
SMRiP15257. Positions 1-31, 85-286.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi246939. 1 interaction.
STRINGi10116.ENSRNOP00000001565.

PTM databases

iPTMnetiP15257.
PhosphoSiteiP15257.

Proteomic databases

PaxDbiP15257.
PRIDEiP15257.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSRNOT00000001565; ENSRNOP00000001565; ENSRNOG00000001183.
GeneIDi24817.
KEGGirno:24817.

Organism-specific databases

CTDi6927.
RGDi3828. Hnf1a.

Phylogenomic databases

eggNOGiENOG410IFA0. Eukaryota.
ENOG410ZZZ0. LUCA.
GeneTreeiENSGT00730000110937.
HOGENOMiHOG000015305.
HOVERGENiHBG005980.
InParanoidiP15257.
KOiK08036.
OMAiQSHVAQS.
OrthoDBiEOG091G052F.
PhylomeDBiP15257.
TreeFamiTF320327.

Miscellaneous databases

EvolutionaryTraceiP15257.
PROiP15257.

Gene expression databases

BgeeiENSRNOG00000001183.
GenevisibleiP15257. RN.

Family and domain databases

Gene3Di1.10.10.60. 1 hit.
1.10.260.40. 1 hit.
InterProiIPR006899. HNF-1_N.
IPR023219. HNF1_dimer_dom.
IPR006898. HNF1a_C.
IPR006897. HNF1b_C.
IPR001356. Homeobox_dom.
IPR009057. Homeodomain-like.
IPR010982. Lambda_DNA-bd_dom.
[Graphical view]
PfamiPF04814. HNF-1_N. 1 hit.
PF04813. HNF-1A_C. 1 hit.
PF04812. HNF-1B_C. 1 hit.
PF00046. Homeobox. 1 hit.
[Graphical view]
SMARTiSM00389. HOX. 1 hit.
[Graphical view]
SUPFAMiSSF100957. SSF100957. 1 hit.
SSF46689. SSF46689. 1 hit.
SSF47413. SSF47413. 1 hit.
PROSITEiPS00027. HOMEOBOX_1. 1 hit.
PS50071. HOMEOBOX_2. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiHNF1A_RAT
AccessioniPrimary (citable) accession number: P15257
Entry historyi
Integrated into UniProtKB/Swiss-Prot: April 1, 1990
Last sequence update: April 1, 1990
Last modified: September 7, 2016
This is version 164 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

Documents

  1. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.