Reviewed, UniProtKB/Swiss-Prot P15254 (PUR4_ECOLI)

Names and origin

Protein names

Recommended name:
    Phosphoribosylformylglycinamidine synthase
      Short name=FGAM synthase
      Short name=FGAMS
    EC=6.3.5.3
Alternative name(s):
    Formylglycinamide ribotide amidotransferase
      Short name=FGARAT
    Formylglycinamide ribotide synthetase

Gene names

Name:	purL
Synonyms:	purI
Ordered Locus Names:	b2557, JW2541

Organism

Escherichia coli (strain K12) [Complete proteome] [HAMAP]

Taxonomic identifier

83333 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Gammaproteobacteria › Enterobacteriales › Enterobacteriaceae › Escherichia

Protein attributes

Sequence length	1295 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Evidence at protein level.

General annotation (Comments)

Catalytic activity	ATP + N(2)-formyl-N(1)-(5-phospho-D-ribosyl)glycinamide + L-glutamine + H2O = ADP + phosphate + 2-(formamido)-N(1)-(5-phospho-D-ribosyl)acetamidine + L-glutamate. HAMAP MF_00419
Pathway	Purine metabolism; IMP biosynthesis via de novo pathway; 5-amino-1-(5-phospho-D-ribosyl)imidazole from N(2)-formyl-N(1)-(5-phospho-D-ribosyl)glycinamide: step 1/2. HAMAP MF_00419
Subunit structure	Monomer. Ref.1
Subcellular location	Cytoplasm. HAMAP MF_00419
Post-translational modification	Both N-terminus methionine truncation and retention have been observed for this protein (Ref.1). HAMAP MF_00419
Sequence similarities	In the N-terminal section; belongs to the FGAMS family. Contains 1 glutamine amidotransferase type-1 domain.
Biophysicochemical properties	Kinetic parameters: KM=64 µM for glutamine HAMAP MF_00419 KM=51 µM for ATP KM=30 µM for FGAR (formylglycinamide ribonucleotide) pH dependence: Optimum pH is 7.2.

Ontologies

Keywords
Biological process	Purine biosynthesis
Cellular component	Cytoplasm
Domain	Glutamine amidotransferase
Ligand	ATP-binding Nucleotide-binding
Molecular function	Ligase
Technical term	Complete proteome Direct protein sequencing
Gene Ontology (GO)
Biological process	'de novo' IMP biosynthetic process Inferred from electronic annotation. Source: InterPro glutamine metabolic process Inferred from electronic annotation. Source: UniProtKB-KW
Cellular component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	ATP binding Inferred from electronic annotation. Source: HAMAP phosphoribosylformylglycinamidine synthase activity Inferred from electronic annotation. Source: HAMAP
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 1295	1295	Phosphoribosylformylglycinamidine synthase HAMAP MF_00419		PRO_0000100406
Regions
	Domain	1042 – 1295	254	Glutamine amidotransferase type-1
	Nucleotide binding	307 – 318	12	ATP Potential
Sites
	Active site	1135	1	Nucleophile By similarity
	Active site	1260	1	By similarity
	Active site	1262	1	By similarity

Sequences

	Sequence		Length	Mass (Da)
	P15254-1 [UniParc]. Last modified December 4, 2007. Version 3. Checksum: 30943AA218D758FE	FASTA	1,295	141,403
	10 20 30 40 50 60 MMEILRGSPA LSAFRINKLL ARFQAARLPV HNIYAEYVHF ADLNAPLNDD EHAQLERLLK 70 80 90 100 110 120 YGPALASHAP QGKLLLVTPR PGTISPWSSK ATDIAHNCGL QQVNRLERGV AYYIEAGTLT 130 140 150 160 170 180 NEQWQQVTAE LHDRMMETVF FALDDAEQLF AHHQPTPVTS VDLLGQGRQA LIDANLRLGL 190 200 210 220 230 240 ALAEDEIDYL QDAFTKLGRN PNDIELYMFA QANSEHCRHK IFNADWVIDG EQQPKSLFKM 250 260 270 280 290 300 IKNTFETTPD HVLSAYKDNA AVMEGSEVGR YFADHETGRY DFHQEPAHIL MKVETHNHPT 310 320 330 340 350 360 AISPWPGAAT GSGGEIRDEG ATGRGAKPKA GLVGFSVSNL RIPGFEQPWE EDFGKPERIV 370 380 390 400 410 420 TALDIMTEGP LGGAAFNNEF GRPALNGYFR TYEEKVNSHN GEELRGYHKP IMLAGGIGNI 430 440 450 460 470 480 RADHVQKGEI NVGAKLVVLG GPAMNIGLGG GAASSMASGQ SDADLDFASV QRDNPEMERR 490 500 510 520 530 540 CQEVIDRCWQ LGDANPILFI HDVGAGGLSN AMPELVSDGG RGGKFELREI LSDEPGMSPL 550 560 570 580 590 600 EIWCNESQER YVLAVAADQL PLFDELCKRE RAPYAVIGEA TEELHLSLHD RHFDNQPIDL 610 620 630 640 650 660 PLDVLLGKTP KMTRDVQTLK AKGDALAREG ITIADAVKRV LHLPTVAEKT FLVTIGDRSV 670 680 690 700 710 720 TGMVARDQMV GPWQVPVANC AVTTASLDSY YGEAMAIGER APVALLDFAA SARLAVGEAL 730 740 750 760 770 780 TNIAATQIGD IKRIKLSANW MAAAGHPGED AGLYEAVKAV GEELCPALGL TIPVGKDSMS 790 800 810 820 830 840 MKTRWQEGNE EREMTSPLSL VISAFARVED VRHTITPQLS TEDNALLLID LGKGNNALGA 850 860 870 880 890 900 TALAQVYRQL GDKPADVRDV AQLKGFYDAI QALVAQRKLL AYHDRSDGGL LVTLAEMAFA 910 920 930 940 950 960 GHCGIDADIA TLGDDRLAAL FNEELGAVIQ VRAADREAVE SVLAQHGLAD CVHYVGQAVS 970 980 990 1000 1010 1020 GDRFVITANG QTVFSESRTT LRVWWAETTW QMQRLRDNPE CADQEHQAKS NDADPGLNVK 1030 1040 1050 1060 1070 1080 LSFDINEDVA APYIATGARP KVAVLREQGV NSHVEMAAAF HRAGFDAIDV HMSDLLTGRT 1090 1100 1110 1120 1130 1140 GLEDFHALVA CGGFSYGDVL GAGEGWAKSI LFNDRVRDEF ATFFHRPQTL ALGVCNGCQM 1150 1160 1170 1180 1190 1200 MSNLRELIPG SELWPRFVRN TSDRFEARFS LVEVTQSPSL LLQGMVGSQM PIAVSHGEGR 1210 1220 1230 1240 1250 1260 VEVRDAAHLA ALESKGLVAL RYVDNFGKVT ETYPANPNGS PNGITAVTTE SGRVTIMMPH 1270 1280 1290 PERVFRTVSN SWHPENWGED GPWMRIFRNA RKQLG

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Formylglycinamide ribonucleotide synthetase from Escherichia coli: cloning, sequencing, overproduction, isolation, and characterization." Schendel F.J., Mueller E., Stubbe J., Shiau A., Smith J.M. Biochemistry 28:2459-2471(1989) [PubMed: 2659070] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA], PROTEIN SEQUENCE OF 1-13, SUBUNIT, CHARACTERIZATION.
[2]	"The organization of the purL gene encoding 5'-phosphoribosylformylglycinamide amidotransferase of Escherichia coli." Sampei G., Mizobuchi K. J. Biol. Chem. 264:21230-21238(1989) [PubMed: 2531746] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
[3]	"The complete genome sequence of Escherichia coli K-12." Blattner F.R., Plunkett G. III, Bloch C.A., Perna N.T., Burland V., Riley M., Collado-Vides J., Glasner J.D., Rode C.K., Mayhew G.F., Gregor J., Davis N.W., Kirkpatrick H.A., Goeden M.A., Rose D.J., Mau B., Shao Y. Science 277:1453-1474(1997) [PubMed: 9278503] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: K12 / MG1655 / ATCC 47076.
[4]	"Escherichia coli K-12: a cooperatively developed annotation snapshot -- 2005." Riley M., Abe T., Arnaud M.B., Berlyn M.K.B., Blattner F.R., Chaudhuri R.R., Glasner J.D., Horiuchi T., Keseler I.M., Kosuge T., Mori H., Perna N.T., Plunkett G. III, Rudd K.E., Serres M.H., Thomas G.H., Thomson N.R., Wishart D., Wanner B.L. Nucleic Acids Res. 34:1-9(2006) [PubMed: 16397293] [Abstract] Cited for: SEQUENCE REVISION TO 847.
[5]	"Highly accurate genome sequences of Escherichia coli K-12 strains MG1655 and W3110." Hayashi K., Morooka N., Yamamoto Y., Fujita K., Isono K., Choi S., Ohtsubo E., Baba T., Wanner B.L., Mori H., Horiuchi T. Mol. Syst. Biol. 2:E1-E5(2006) [PubMed: 16738553] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: K12 / W3110 / ATCC 27325 / DSM 5911.

Cross-references

Sequence databases
EMBLGenBankDDBJ	M19501 Genomic DNA. Translation: AAA24456.1. U36841 Genomic DNA. Translation: AAA79819.1. U00096 Genomic DNA. Translation: AAT48143.1. AP009048 Genomic DNA. Translation: BAE76733.1.
PIR	SYECPG. D65033.
RefSeq	AP_003143.1. YP_026170.1.
3D structure databases
SMR	P15254. Positions 1-1294.
ModBase	Search...
Protein-protein interaction databases
DIP	DIP:10614N.
IntAct	P15254. 8 interactions.
STRING	P15254.
Genome annotation databases
GeneID	947032.
GenomeReviews	Gene locus JW2541 in contig AP009048_GR. Gene locus b2557 in contig U00096_GR.
KEGG	ecj:JW2541. eco:b2557.
Organism-specific databases
EchoBASE	EB0790.
EcoGene	EG10797. purL.
CMR	Search...
Phylogenomic databases
HOGENOM	P15254.
OMA	ERGIAYY.
Enzyme and pathway databases
BioCyc	EcoCyc:FGAMSYN-MON. MetaCyc:FGAMSYN-MON.
Gene expression databases
Genevestigator	P15254.
Family and domain databases
HAMAP	MF_00419. [Tree]
InterPro	IPR000728. AIR_synth. IPR010918. AIR_synth_C. IPR017926. GATASE_1. IPR010073. PRibForGlyAmidine_synth. [Graphical view]
Pfam	PF00586. AIRS. 1 hit. PF02769. AIRS_C. 2 hits. [Graphical view]
TIGRFAMs	TIGR01735. FGAM_synt. 1 hit.
PROSITE	PS51273. GATASE_TYPE_1. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

PUR4_ECOLI

Accession

Primary (citable) accession number: P15254
Secondary accession number(s): P78097, Q2MAH3

Entry history

Integrated into UniProtKB/Swiss-Prot:	April 1, 1990
Last sequence update:	December 4, 2007
Last modified:	November 3, 2009
This is version 102 of the entry and version 3 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)