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Protein

Protein-L-isoaspartate(D-aspartate) O-methyltransferase

Gene

PCMT1

Organism
Bos taurus (Bovine)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Experimental evidence at protein leveli

Functioni

Catalyzes the methyl esterification of L-isoaspartyl and D-aspartyl residues in peptides and proteins that result from spontaneous decomposition of normal L-aspartyl and L-asparaginyl residues. It plays a role in the repair and/or degradation of damaged proteins. Acts on EIF4EBP2, microtubule-associated protein 2, calreticulin, clathrin light chains a and b, Ubiquitin carboxyl-terminal hydrolase isozyme L1, phosphatidylethanolamine-binding protein 1, stathmin, beta-synuclein and alpha-synuclein.By similarity

Catalytic activityi

S-adenosyl-L-methionine + protein L-isoaspartate = S-adenosyl-L-homocysteine + protein L-isoaspartate alpha-methyl ester.

GO - Molecular functioni

Keywordsi

Molecular functionMethyltransferase, Transferase
LigandS-adenosyl-L-methionine

Enzyme and pathway databases

ReactomeiR-BTA-5676934. Protein repair.

Names & Taxonomyi

Protein namesi
Recommended name:
Protein-L-isoaspartate(D-aspartate) O-methyltransferase (EC:2.1.1.77)
Short name:
PIMT
Alternative name(s):
L-isoaspartyl protein carboxyl methyltransferase
Protein L-isoaspartyl/D-aspartyl methyltransferase
Protein-beta-aspartate methyltransferase
Gene namesi
Name:PCMT1
OrganismiBos taurus (Bovine)
Taxonomic identifieri9913 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaLaurasiatheriaCetartiodactylaRuminantiaPecoraBovidaeBovinaeBos
Proteomesi
  • UP000009136 Componenti: Chromosome 9

Subcellular locationi

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Initiator methionineiRemovedBy similarity
ChainiPRO_00001118742 – 227Protein-L-isoaspartate(D-aspartate) O-methyltransferaseAdd BLAST226

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei2N-acetylalanineBy similarity1
Disulfide bondi43 ↔ 95

Keywords - PTMi

Acetylation, Disulfide bond

Proteomic databases

PaxDbiP15246.
PeptideAtlasiP15246.
PRIDEiP15246.

Expressioni

Gene expression databases

BgeeiENSBTAG00000010204.
ExpressionAtlasiP15246. baseline.

Interactioni

Subunit structurei

Monomer.

Protein-protein interaction databases

STRINGi9913.ENSBTAP00000055150.

Structurei

3D structure databases

ProteinModelPortaliP15246.
SMRiP15246.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Phylogenomic databases

eggNOGiKOG1661. Eukaryota.
COG2518. LUCA.
GeneTreeiENSGT00510000046974.
HOGENOMiHOG000257189.
HOVERGENiHBG004483.
InParanoidiP15246.
KOiK00573.
OMAiPYTVAFQ.

Family and domain databases

InterProiView protein in InterPro
IPR000682. PCMT.
IPR029063. SAM-dependent_MTases.
PANTHERiPTHR11579. PTHR11579. 1 hit.
SUPFAMiSSF53335. SSF53335. 1 hit.
TIGRFAMsiTIGR00080. pimt. 1 hit.
PROSITEiView protein in PROSITE
PS01279. PCMT. 1 hit.

Sequences (2)i

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

This entry describes 2 isoformsi produced by alternative splicing. AlignAdd to basket

Isoform 1 (identifier: P15246-1) [UniParc]FASTAAdd to basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MAWKSGGASH SELIHNLRKN GIIKTDKVFE VMLATDRSHY AKCNPYMDSP
60 70 80 90 100
QSIGFQATIS APHMHAYALE LLFDQLNEGA KALDVGSGSG ILTACFARMV
110 120 130 140 150
GPSGKVIGID HIKELVDDSI NNVRKDDPML LSSGRVQLVV GDGRMGYAAE
160 170 180 190 200
APYDAIHVGA AAPVVPQALI DQLKPGGRLI LPVGPAGGNQ MLEQYDKLQD
210 220
GSVKMKPLMG VIYVPLTDKE KQWSRWK
Length:227
Mass (Da):24,565
Last modified:January 23, 2007 - v2
Checksum:i8566921A7B7AFE37
GO
Isoform 2 (identifier: P15246-2) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     226-227: WK → DEL

Show »
Length:228
Mass (Da):24,608
Checksum:i4EDD5F421A7B7AFE
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti133S → T in AAI09664 (Ref. 1) Curated1

Alternative sequence

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Alternative sequenceiVSP_012044226 – 227WK → DEL in isoform 2. Curated2

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
BC109663 mRNA. Translation: AAI09664.1.
PIRiA43292.
RefSeqiNP_001073085.1. NM_001079617.2.
UniGeneiBt.22615.

Genome annotation databases

EnsembliENSBTAT00000013464; ENSBTAP00000013464; ENSBTAG00000010204. [P15246-1]
GeneIDi613854.
KEGGibta:613854.

Keywords - Coding sequence diversityi

Alternative splicing

Similar proteinsi

Entry informationi

Entry nameiPIMT_BOVIN
AccessioniPrimary (citable) accession number: P15246
Secondary accession number(s): Q32LB2
Entry historyiIntegrated into UniProtKB/Swiss-Prot: April 1, 1990
Last sequence update: January 23, 2007
Last modified: October 25, 2017
This is version 129 of the entry and version 2 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Direct protein sequencing, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families