P15229 (SCX1_BUTSI) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names	Recommended name: Small toxin Alternative name(s): Chlorotoxin-like peptide Bs 8 Short name=Bs8 Peptide I
Organism	Buthus sindicus (Scorpion)
Taxonomic identifier	42519 [NCBI]
Taxonomic lineage	cellular organisms › Eukaryota › Opisthokonta › Metazoa › Eumetazoa › Bilateria › Protostomia › Ecdysozoa › Panarthropoda › Arthropoda › Chelicerata › Arachnida › Scorpiones › Buthida › Buthoidea › Buthidae › Mesobuthus › Mesobuthus tamulus

Protein attributes

Sequence length	35 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	Probable chloride channel ligand, which blocks small-conductance chloride channels By similarity.
Subcellular location	Secreted.
Tissue specificity	Expressed by the venom gland.
Domain	The presence of a 'disulfide through disulfide knot' structurally defines this protein as a knottin By similarity.
Sequence similarities	Belongs to the short scorpion toxin superfamily. Chloride channel inhibitor family.
Mass spectrometry	Molecular mass is 3745.4 Da from positions 1 - 35. Determined by MALDI. Ref.2

Ontologies

Keywords
Cellular component	Secreted
Domain	Knottin
Molecular function	Chloride channel inhibitor Ion channel impairing toxin Neurotoxin Toxin
PTM	Disulfide bond
Technical term	Direct protein sequencing
Gene Ontology (GO)
Biological_process	defense response Inferred from electronic annotation. Source: InterPro pathogenesis Inferred from electronic annotation. Source: InterPro
Cellular_component	extracellular region Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular_function	chloride channel inhibitor activity Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Peptide	1 – 35	35	Small toxin		PRO_0000044940
Amino acid modifications
	Disulfide bond	2 ↔ 19		By similarity
	Disulfide bond	5 ↔ 28		By similarity
	Disulfide bond	16 ↔ 33		By similarity
	Disulfide bond	20 ↔ 35		By similarity

Sequences

	Sequence		Length	Mass (Da)
	P15229 [UniParc]. Last modified April 1, 1990. Version 1. Checksum: 7E16A1524D6B83B8	FASTA	35	3,746
	10 20 30 RCKPCFTTDP QMSKKCADCC GGKGKGKCYG PQCLC

References

[1]	"Characterization of two different peptides from the venom of the scorpion Buthus sindicus." Fazal A., Beg O.U., Shafqat J., Zaidi Z.H., Joernvall H. FEBS Lett. 257:260-262(1989) [PubMed] [Europe PMC] [Abstract] Cited for: PROTEIN SEQUENCE. Tissue: Venom.
[2]	"Purification and primary structure of low molecular mass peptides from scorpion (Buthus sindicus) venom." Ali S.A., Stoeva S., Schuetz J., Kayed R., Abbasi A., Zaidi Z.H., Voelter W. Comp. Biochem. Physiol. 121A:323-332(1998) [PubMed] [Europe PMC] [Abstract] Cited for: PROTEIN SEQUENCE, MASS SPECTROMETRY. Tissue: Venom.

Cross-references

Sequence databases
PIR	S06667.
3D structure databases
ModBase	Search...
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Family and domain databases
InterPro	IPR003614. Scorpion_toxin-like. IPR007958. Scorpion_toxinS_Cl_inh. [Graphical view]
Pfam	PF05294. Toxin_5. 1 hit. [Graphical view]
SUPFAM	SSF57095. SSF57095. 1 hit.
PROSITE	PS51200. SHORT_SCORPION_CHLORIDE. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

SCX1_BUTSI

Accession

Primary (citable) accession number: P15229

Entry history

Integrated into UniProtKB/Swiss-Prot:	April 1, 1990
Last sequence update:	April 1, 1990
Last modified:	May 29, 2013
This is version 64 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Animal Toxin Annotation Program