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P15228

- AEP1_MESMA

UniProt

P15228 - AEP1_MESMA

Protein

Toxin BmKAEP

Gene
N/A
Organism
Mesobuthus martensii (Manchurian scorpion) (Buthus martensii)
Status
Reviewed - Annotation score: 4 out of 5- Experimental evidence at protein leveli
  1. Functioni

    Shows anti-epileptic activity. Shares high homology with depressant insect toxins, but shows very weak toxicity against mammals and insects and no obvious symptoms on insect larvae. May target voltage-gated sodium channel (Nav).2 Publications

    GO - Molecular functioni

    1. sodium channel inhibitor activity Source: UniProtKB

    GO - Biological processi

    1. defense response Source: InterPro
    2. pathogenesis Source: UniProtKB

    Keywords - Molecular functioni

    Ion channel impairing toxin, Neurotoxin, Toxin, Voltage-gated sodium channel impairing toxin

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Toxin BmKAEP
    Alternative name(s):
    Anti-epilepsy peptide
    BmK AEP
    OrganismiMesobuthus martensii (Manchurian scorpion) (Buthus martensii)
    Taxonomic identifieri34649 [NCBI]
    Taxonomic lineageiEukaryotaMetazoaEcdysozoaArthropodaChelicerataArachnidaScorpionesButhidaButhoideaButhidaeMesobuthus

    Subcellular locationi

    GO - Cellular componenti

    1. extracellular region Source: UniProtKB

    Keywords - Cellular componenti

    Secreted

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Signal peptidei1 – 21211 PublicationAdd
    BLAST
    Chaini22 – 8261Toxin BmKAEPPRO_0000035212Add
    BLAST

    Amino acid modifications

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Disulfide bondi31 ↔ 81By similarity
    Disulfide bondi35 ↔ 56By similarity
    Disulfide bondi42 ↔ 63By similarity
    Disulfide bondi46 ↔ 65By similarity
    Modified residuei82 – 821Glycine amide

    Keywords - PTMi

    Amidation, Disulfide bond

    Expressioni

    Tissue specificityi

    Expressed by the venom gland.

    Structurei

    3D structure databases

    ProteinModelPortaliP15228.
    SMRiP15228. Positions 22-82.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Sequence similaritiesi

    Keywords - Domaini

    Signal

    Family and domain databases

    Gene3Di3.30.30.10. 1 hit.
    InterProiIPR003614. Scorpion_toxin-like.
    IPR018218. Scorpion_toxinL.
    IPR002061. Scorpion_toxinL/defensin.
    [Graphical view]
    PfamiPF00537. Toxin_3. 1 hit.
    [Graphical view]
    PRINTSiPR00285. SCORPNTOXIN.
    SMARTiSM00505. Knot1. 1 hit.
    [Graphical view]
    SUPFAMiSSF57095. SSF57095. 1 hit.

    Sequencei

    Sequence statusi: Complete.

    Sequence processingi: The displayed sequence is further processed into a mature form.

    P15228-1 [UniParc]FASTAAdd to Basket

    « Hide

    MKLFLLLVIS ASMLIDGLVN ADGYIRGSNG CKVSCLLGNE GCNKECRAYG   50
    ASYGYCWTWK LACWCQGLPD DKTWKSESNT CGGKK 85
    Length:85
    Mass (Da):9,296
    Last modified:October 24, 2003 - v2
    Checksum:iCC321DADAAEEF35E
    GO

    Experimental Info

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Sequence conflicti29 – 302NG → DN AA sequence (PubMed:2930463)Curated
    Sequence conflicti59 – 591W → V AA sequence (PubMed:2930463)Curated
    Sequence conflicti63 – 642CW → QD AA sequence (PubMed:2930463)Curated
    Sequence conflicti66 – 661Q → E AA sequence (PubMed:11298767)Curated
    Sequence conflicti66 – 661Q → E AA sequence (PubMed:2930463)Curated
    Sequence conflicti71 – 711D → T AA sequence (PubMed:2930463)Curated

    Mass spectrometryi

    Molecular mass is 6730.4 Da from positions 22 - 82. Determined by ESI. 1 Publication

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AF160868 mRNA. Translation: AAD43570.2.
    PIRiS02174.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AF160868 mRNA. Translation: AAD43570.2 .
    PIRi S02174.

    3D structure databases

    ProteinModelPortali P15228.
    SMRi P15228. Positions 22-82.
    ModBasei Search...
    MobiDBi Search...

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Family and domain databases

    Gene3Di 3.30.30.10. 1 hit.
    InterProi IPR003614. Scorpion_toxin-like.
    IPR018218. Scorpion_toxinL.
    IPR002061. Scorpion_toxinL/defensin.
    [Graphical view ]
    Pfami PF00537. Toxin_3. 1 hit.
    [Graphical view ]
    PRINTSi PR00285. SCORPNTOXIN.
    SMARTi SM00505. Knot1. 1 hit.
    [Graphical view ]
    SUPFAMi SSF57095. SSF57095. 1 hit.
    ProtoNeti Search...

    Publicationsi

    1. "Molecular characterization of an anti-epilepsy peptide from the scorpion Buthus martensi Karsch."
      Wang C.-G., He X.-L., Shao F., Liu W., Ling M.-H., Wang D.-C., Chi C.-W.
      Eur. J. Biochem. 268:2480-2485(2001) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [MRNA], PROTEIN SEQUENCE OF 22-75, FUNCTION, MASS SPECTROMETRY.
      Tissue: Venom and Venom gland.
    2. "Purification and N-terminal partial sequence of anti-epilepsy peptide from venom of the scorpion Buthus martensii Karsch."
      Zhou X.-H., Yang D., Zhang J.-H., Liu C.-M., Lei K.-J.
      Biochem. J. 257:509-517(1989) [PubMed] [Europe PMC] [Abstract]
      Cited for: PRELIMINARY PROTEIN SEQUENCE OF 22-71, FUNCTION.
      Tissue: Venom.

    Entry informationi

    Entry nameiAEP1_MESMA
    AccessioniPrimary (citable) accession number: P15228
    Secondary accession number(s): Q9Y0B8
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: April 1, 1990
    Last sequence update: October 24, 2003
    Last modified: October 1, 2014
    This is version 76 of the entry and version 2 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programAnimal Toxin Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Direct protein sequencing

    Documents

    1. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3