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P15228 (AEP1_MESMA) Reviewed, UniProtKB/Swiss-Prot

Last modified March 19, 2014. Version 75. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (1) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Toxin BmKAEP
Alternative name(s):
Anti-epilepsy peptide
BmK AEP
OrganismMesobuthus martensii (Manchurian scorpion) (Buthus martensii)
Taxonomic identifier34649 [NCBI]
Taxonomic lineageEukaryotaMetazoaEcdysozoaArthropodaChelicerataArachnidaScorpionesButhidaButhoideaButhidaeMesobuthus

Protein attributes

Sequence length85 AA.
Sequence statusComplete.
Sequence processingThe displayed sequence is further processed into a mature form.
Protein existenceEvidence at protein level

General annotation (Comments)

Function

Shows anti-epileptic activity. Shares high homology with depressant insect toxins, but shows very weak toxicity against mammals and insects and no obvious symptoms on insect larvae. May target voltage-gated sodium channel (Nav). Ref.1 Ref.2

Subcellular location

Secreted.

Tissue specificity

Expressed by the venom gland.

Sequence similarities

Belongs to the long (4 C-C) scorpion toxin superfamily. Sodium channel inhibitor family. Beta subfamily.

Mass spectrometry

Molecular mass is 6730.4 Da from positions 22 - 82. Determined by ESI. Ref.1

Ontologies

Keywords
   Cellular componentSecreted
   DomainSignal
   Molecular functionIon channel impairing toxin
Neurotoxin
Toxin
Voltage-gated sodium channel impairing toxin
   PTMAmidation
Disulfide bond
   Technical termDirect protein sequencing
Gene Ontology (GO)
   Biological_processdefense response

Inferred from electronic annotation. Source: InterPro

pathogenesis

Inferred from direct assay Ref.2. Source: UniProtKB

   Cellular_componentextracellular region

Inferred from direct assay Ref.2. Source: UniProtKB

   Molecular_functionsodium channel inhibitor activity

Non-traceable author statement Ref.1. Source: UniProtKB

Complete GO annotation...

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Signal peptide1 – 2121 Ref.1 Ref.2
Chain22 – 8261Toxin BmKAEP
PRO_0000035212

Amino acid modifications

Modified residue821Glycine amide
Disulfide bond31 ↔ 81 By similarity
Disulfide bond35 ↔ 56 By similarity
Disulfide bond42 ↔ 63 By similarity
Disulfide bond46 ↔ 65 By similarity

Experimental info

Sequence conflict29 – 302NG → DN AA sequence Ref.2
Sequence conflict591W → V AA sequence Ref.2
Sequence conflict63 – 642CW → QD AA sequence Ref.2
Sequence conflict661Q → E AA sequence Ref.1
Sequence conflict661Q → E AA sequence Ref.2
Sequence conflict711D → T AA sequence Ref.2

Sequences

Sequence LengthMass (Da)Tools
P15228 [UniParc].

Last modified October 24, 2003. Version 2.
Checksum: CC321DADAAEEF35E

FASTA859,296
        10         20         30         40         50         60 
MKLFLLLVIS ASMLIDGLVN ADGYIRGSNG CKVSCLLGNE GCNKECRAYG ASYGYCWTWK 

        70         80 
LACWCQGLPD DKTWKSESNT CGGKK 

« Hide

References

[1]"Molecular characterization of an anti-epilepsy peptide from the scorpion Buthus martensi Karsch."
Wang C.-G., He X.-L., Shao F., Liu W., Ling M.-H., Wang D.-C., Chi C.-W.
Eur. J. Biochem. 268:2480-2485(2001) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA], PROTEIN SEQUENCE OF 22-75, FUNCTION, MASS SPECTROMETRY.
Tissue: Venom and Venom gland.
[2]"Purification and N-terminal partial sequence of anti-epilepsy peptide from venom of the scorpion Buthus martensii Karsch."
Zhou X.-H., Yang D., Zhang J.-H., Liu C.-M., Lei K.-J.
Biochem. J. 257:509-517(1989) [PubMed] [Europe PMC] [Abstract]
Cited for: PRELIMINARY PROTEIN SEQUENCE OF 22-71, FUNCTION.
Tissue: Venom.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
AF160868 mRNA. Translation: AAD43570.2.
PIRS02174.

3D structure databases

ProteinModelPortalP15228.
SMRP15228. Positions 22-82.
ModBaseSearch...
MobiDBSearch...

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Family and domain databases

Gene3D3.30.30.10. 1 hit.
InterProIPR003614. Scorpion_toxin-like.
IPR018218. Scorpion_toxinL.
IPR002061. Scorpion_toxinL/defensin.
[Graphical view]
PfamPF00537. Toxin_3. 1 hit.
[Graphical view]
PRINTSPR00285. SCORPNTOXIN.
SMARTSM00505. Knot1. 1 hit.
[Graphical view]
SUPFAMSSF57095. SSF57095. 1 hit.
ProtoNetSearch...

Entry information

Entry nameAEP1_MESMA
AccessionPrimary (citable) accession number: P15228
Secondary accession number(s): Q9Y0B8
Entry history
Integrated into UniProtKB/Swiss-Prot: April 1, 1990
Last sequence update: October 24, 2003
Last modified: March 19, 2014
This is version 75 of the entry and version 2 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programAnimal Toxin Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families