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P15228

- AEP1_MESMA

UniProt

P15228 - AEP1_MESMA

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Protein

Toxin BmKAEP

Gene
N/A
Organism
Mesobuthus martensii (Manchurian scorpion) (Buthus martensii)
Status
Reviewed - Annotation score: 4 out of 5- Experimental evidence at protein leveli

Functioni

Shows anti-epileptic activity. Shares high homology with depressant insect toxins, but shows very weak toxicity against mammals and insects and no obvious symptoms on insect larvae. May target voltage-gated sodium channel (Nav).2 Publications

GO - Molecular functioni

  1. sodium channel inhibitor activity Source: UniProtKB

GO - Biological processi

  1. defense response Source: InterPro
  2. pathogenesis Source: UniProtKB
Complete GO annotation...

Keywords - Molecular functioni

Ion channel impairing toxin, Neurotoxin, Toxin, Voltage-gated sodium channel impairing toxin

Names & Taxonomyi

Protein namesi
Recommended name:
Toxin BmKAEP
Alternative name(s):
Anti-epilepsy peptide
BmK AEP
OrganismiMesobuthus martensii (Manchurian scorpion) (Buthus martensii)
Taxonomic identifieri34649 [NCBI]
Taxonomic lineageiEukaryotaMetazoaEcdysozoaArthropodaChelicerataArachnidaScorpionesButhidaButhoideaButhidaeMesobuthus

Subcellular locationi

GO - Cellular componenti

  1. extracellular region Source: UniProtKB
Complete GO annotation...

Keywords - Cellular componenti

Secreted

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Signal peptidei1 – 21211 PublicationAdd
BLAST
Chaini22 – 8261Toxin BmKAEPPRO_0000035212Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Disulfide bondi31 ↔ 81By similarity
Disulfide bondi35 ↔ 56By similarity
Disulfide bondi42 ↔ 63By similarity
Disulfide bondi46 ↔ 65By similarity
Modified residuei82 – 821Glycine amide

Keywords - PTMi

Amidation, Disulfide bond

Expressioni

Tissue specificityi

Expressed by the venom gland.

Structurei

3D structure databases

ProteinModelPortaliP15228.
SMRiP15228. Positions 22-82.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Keywords - Domaini

Signal

Family and domain databases

Gene3Di3.30.30.10. 1 hit.
InterProiIPR003614. Scorpion_toxin-like.
IPR018218. Scorpion_toxinL.
IPR002061. Scorpion_toxinL/defensin.
[Graphical view]
PfamiPF00537. Toxin_3. 1 hit.
[Graphical view]
PRINTSiPR00285. SCORPNTOXIN.
SMARTiSM00505. Knot1. 1 hit.
[Graphical view]
SUPFAMiSSF57095. SSF57095. 1 hit.

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

P15228-1 [UniParc]FASTAAdd to Basket

« Hide

        10         20         30         40         50
MKLFLLLVIS ASMLIDGLVN ADGYIRGSNG CKVSCLLGNE GCNKECRAYG
60 70 80
ASYGYCWTWK LACWCQGLPD DKTWKSESNT CGGKK
Length:85
Mass (Da):9,296
Last modified:October 24, 2003 - v2
Checksum:iCC321DADAAEEF35E
GO

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti29 – 302NG → DN AA sequence (PubMed:2930463)Curated
Sequence conflicti59 – 591W → V AA sequence (PubMed:2930463)Curated
Sequence conflicti63 – 642CW → QD AA sequence (PubMed:2930463)Curated
Sequence conflicti66 – 661Q → E AA sequence (PubMed:11298767)Curated
Sequence conflicti66 – 661Q → E AA sequence (PubMed:2930463)Curated
Sequence conflicti71 – 711D → T AA sequence (PubMed:2930463)Curated

Mass spectrometryi

Molecular mass is 6730.4 Da from positions 22 - 82. Determined by ESI. 1 Publication

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
AF160868 mRNA. Translation: AAD43570.2.
PIRiS02174.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
AF160868 mRNA. Translation: AAD43570.2 .
PIRi S02174.

3D structure databases

ProteinModelPortali P15228.
SMRi P15228. Positions 22-82.
ModBasei Search...
MobiDBi Search...

Protocols and materials databases

Structural Biology Knowledgebase Search...

Family and domain databases

Gene3Di 3.30.30.10. 1 hit.
InterProi IPR003614. Scorpion_toxin-like.
IPR018218. Scorpion_toxinL.
IPR002061. Scorpion_toxinL/defensin.
[Graphical view ]
Pfami PF00537. Toxin_3. 1 hit.
[Graphical view ]
PRINTSi PR00285. SCORPNTOXIN.
SMARTi SM00505. Knot1. 1 hit.
[Graphical view ]
SUPFAMi SSF57095. SSF57095. 1 hit.
ProtoNeti Search...

Publicationsi

  1. "Molecular characterization of an anti-epilepsy peptide from the scorpion Buthus martensi Karsch."
    Wang C.-G., He X.-L., Shao F., Liu W., Ling M.-H., Wang D.-C., Chi C.-W.
    Eur. J. Biochem. 268:2480-2485(2001) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA], PROTEIN SEQUENCE OF 22-75, FUNCTION, MASS SPECTROMETRY.
    Tissue: Venom and Venom gland.
  2. "Purification and N-terminal partial sequence of anti-epilepsy peptide from venom of the scorpion Buthus martensii Karsch."
    Zhou X.-H., Yang D., Zhang J.-H., Liu C.-M., Lei K.-J.
    Biochem. J. 257:509-517(1989) [PubMed] [Europe PMC] [Abstract]
    Cited for: PRELIMINARY PROTEIN SEQUENCE OF 22-71, FUNCTION.
    Tissue: Venom.

Entry informationi

Entry nameiAEP1_MESMA
AccessioniPrimary (citable) accession number: P15228
Secondary accession number(s): Q9Y0B8
Entry historyi
Integrated into UniProtKB/Swiss-Prot: April 1, 1990
Last sequence update: October 24, 2003
Last modified: October 1, 2014
This is version 76 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programAnimal Toxin Annotation Program

Miscellaneousi

Keywords - Technical termi

Direct protein sequencing

Documents

  1. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3