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P15226

- SCX1_TITSE

UniProt

P15226 - SCX1_TITSE

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Protein
Beta-mammal/insect toxin Ts1
Gene
N/A
Organism
Tityus serrulatus (Brazilian scorpion)
Status
Reviewed - Annotation score: 5 out of 5 - Experimental evidence at protein leveli

Functioni

Beta toxins bind voltage-independently at site-4 of sodium channels (Nav) and shift the voltage of activation toward more negative potentials thereby affecting sodium channel activation and promoting spontaneous and repetitive firing. In addition, it stimulates the release of NO, IL-6 and TNF-alpha in J774.1 cells (1 Publication). This toxin is active against both mammals and insects.1 Publication

GO - Molecular functioni

  1. ion channel inhibitor activity Source: InterPro

GO - Biological processi

  1. defense response Source: InterPro
Complete GO annotation...

Keywords - Molecular functioni

Ion channel impairing toxin, Neurotoxin, Toxin, Voltage-gated sodium channel impairing toxin

Names & Taxonomyi

Protein namesi
Recommended name:
Beta-mammal/insect toxin Ts1
Alternative name(s):
PT-Mice-Ins-beta NaTx6.1
Tityustoxin VII
Short name:
Ts VII
Short name:
Ts7
Short name:
TsTX-VII
Toxin II-11
Toxin III-10
Toxin T2-IV
Toxin gamma
TsTX-I
OrganismiTityus serrulatus (Brazilian scorpion)
Taxonomic identifieri6887 [NCBI]
Taxonomic lineageiEukaryotaMetazoaEcdysozoaArthropodaChelicerataArachnidaScorpionesButhidaButhoideaButhidaeTityus

Subcellular locationi

GO - Cellular componenti

  1. extracellular region Source: UniProtKB-SubCell
Complete GO annotation...

Keywords - Cellular componenti

Secreted

Pathology & Biotechi

Toxic dosei

LD50 is 826 +/- 156 µg/kg by intravenous injection into mice (1 Publication).1 Publication
LD50 is 11 +/- 9 µg/kg by intracistenal injection into mice (1 Publication).1 Publication
LD50 is 0.6 ng, intracerebroventricular.1 Publication

Mutagenesis

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Mutagenesisi81 – 811C → CG: Decrease of affinity of this non amidated toxin (Ts1-G) for sodium channel. 1 Publication

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Signal peptidei1 – 20204 Publications
Add
BLAST
Chaini21 – 8161Beta-mammal/insect toxin Ts1
PRO_0000035305Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Disulfide bondi31 ↔ 811 Publication
Disulfide bondi35 ↔ 571 Publication
Disulfide bondi43 ↔ 621 Publication
Disulfide bondi47 ↔ 641 Publication
Modified residuei81 – 811Cysteine amide

Keywords - PTMi

Amidation, Disulfide bond

Expressioni

Tissue specificityi

Expressed by the venom gland.

Structurei

Secondary structure

Legend: HelixTurnBeta strand
Show more details
Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Helixi42 – 498
Beta strandi53 – 586
Beta strandi61 – 666
Turni76 – 783

3D structure databases

Select the link destinations:
PDBe
RCSB PDB
PDBj
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
1B7DX-ray1.73A21-81[»]
1NPIX-ray1.16A21-81[»]
ProteinModelPortaliP15226.
SMRiP15226. Positions 21-81.

Miscellaneous databases

EvolutionaryTraceiP15226.

Family & Domainsi

Sequence similaritiesi

Keywords - Domaini

Signal

Family and domain databases

Gene3Di3.30.30.10. 1 hit.
InterProiIPR003614. Scorpion_toxin-like.
IPR018218. Scorpion_toxinL.
IPR002061. Scorpion_toxinL/defensin.
[Graphical view]
PfamiPF00537. Toxin_3. 1 hit.
[Graphical view]
PRINTSiPR00285. SCORPNTOXIN.
SMARTiSM00505. Knot1. 1 hit.
[Graphical view]
SUPFAMiSSF57095. SSF57095. 1 hit.

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

P15226-1 [UniParc]FASTAAdd to Basket

« Hide

MKGMILFISC LLLIGIVVEC KEGYLMDHEG CKLSCFIRPS GYCGRECGIK   50
KGSSGYCAWP ACYCYGLPNW VKVWDRATNK CGKK 84
Length:84
Mass (Da):9,382
Last modified:July 1, 1993 - v2
Checksum:i430F3FCA74823E77
GO

Mass spectrometryi

Molecular mass is 6879.4 Da from positions 21 - 81. Determined by MALDI. 1 Publication

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
X66256 mRNA. Translation: CAA46982.1.
S66941 Genomic DNA. Translation: AAB29128.1.
PIRiS21158.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
X66256 mRNA. Translation: CAA46982.1 .
S66941 Genomic DNA. Translation: AAB29128.1 .
PIRi S21158.

3D structure databases

Select the link destinations:
PDBe
RCSB PDB
PDBj
Links Updated
Entry Method Resolution (Å) Chain Positions PDBsum
1B7D X-ray 1.73 A 21-81 [» ]
1NPI X-ray 1.16 A 21-81 [» ]
ProteinModelPortali P15226.
SMRi P15226. Positions 21-81.
ModBasei Search...
MobiDBi Search...

Protocols and materials databases

Structural Biology Knowledgebase Search...

Miscellaneous databases

EvolutionaryTracei P15226.

Family and domain databases

Gene3Di 3.30.30.10. 1 hit.
InterProi IPR003614. Scorpion_toxin-like.
IPR018218. Scorpion_toxinL.
IPR002061. Scorpion_toxinL/defensin.
[Graphical view ]
Pfami PF00537. Toxin_3. 1 hit.
[Graphical view ]
PRINTSi PR00285. SCORPNTOXIN.
SMARTi SM00505. Knot1. 1 hit.
[Graphical view ]
SUPFAMi SSF57095. SSF57095. 1 hit.
ProtoNeti Search...

Publicationsi

  1. "Molecular cloning and nucleotide sequence analysis of a cDNA encoding the main beta-neurotoxin from the venom of the South American scorpion Tityus serrulatus."
    Martin-Eauclaire M.-F., Ceard B., Ribeiro A.M., Diniz C.R., Rochat H., Bougis P.E.
    FEBS Lett. 302:220-222(1992) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA].
    Tissue: Venom gland.
  2. "The genomic region encoding toxin gamma from the scorpion Tityus serrulatus contains an intron."
    Becerril B., Corona M., Mejia M.C., Martin B.M., Lucas S., Bolivar F., Possani L.D.
    FEBS Lett. 335:6-8(1993) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
  3. "Amino acid sequence of toxin VII, a beta-toxin from the venom of the scorpion Tityus serrulatus."
    Bechis G., Sampieri F., Yuan P.-M., Brando T., Martin M.-F., Diniz C.R., Rochat H.
    Biochem. Biophys. Res. Commun. 122:1146-1153(1984) [PubMed] [Europe PMC] [Abstract]
    Cited for: PROTEIN SEQUENCE OF 21-81.
    Tissue: Venom.
  4. "Discharge effect on pancreatic exocrine secretion produced by toxins purified from Tityus serrulatus scorpion venom."
    Possani L.D., Martin B.M., Fletcher M.D., Fletcher P.L. Jr.
    J. Biol. Chem. 266:3178-3185(1991) [PubMed] [Europe PMC] [Abstract]
    Cited for: PROTEIN SEQUENCE OF 21-81.
    Tissue: Venom.
  5. "Purification and chemical characterization of the major toxins from the venom of the Brazilian scorpion Titus serrulatus Lutz and Mello."
    Possani L.D., Martin B.M., Mochca-Morales J., Svendsen I.
    Carlsberg Res. Commun. 46:195-205(1981)
    Cited for: PROTEIN SEQUENCE OF 21-62.
    Tissue: Venom.
  6. "Further characterization of toxins T1IV (TsTX-III) and T2IV from Tityus serrulatus scorpion venom."
    Sampaio S.V., Arantes E.C., Prado W.A., Riccioppo Neto F., Giglio J.R.
    Toxicon 29:663-672(1991) [PubMed] [Europe PMC] [Abstract]
    Cited for: PROTEIN SEQUENCE OF 21-46.
    Tissue: Venom.
  7. "A simplified procedure for the fractionation of Tityus serrulatus venom: isolation and partial characterization of TsTX-IV, a new neurotoxin."
    Arantes E.C., Prado W.A., Sampaio S.V., Giglio J.R.
    Toxicon 27:907-916(1989) [PubMed] [Europe PMC] [Abstract]
    Cited for: AMINO ACID COMPOSITION, LETHAL DOSE.
    Tissue: Venom.
  8. "Tityus serrulatus venom and toxins Ts1, Ts2 and Ts6 induce macrophage activation and production of immune mediators."
    Zoccal K.F., Bitencourt C.S., Secatto A., Sorgi C.A., Bordon K.C., Sampaio S.V., Arantes E.C., Faccioli L.H.
    Toxicon 57:1101-1108(2011) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION.
  9. "Role of lysine and tryptophan residues in the biological activity of toxin VII (Ts gamma) from the scorpion Tityus serrulatus."
    Hassani O., Mansuelle P., Cestele S., Bourdeaux M., Rochat H., Sampieri F.
    Eur. J. Biochem. 260:76-86(1999) [PubMed] [Europe PMC] [Abstract]
    Cited for: IMPORTANCE OF LYS-12; TRP-39 AND TRP-54.
  10. "Functional and structural study comparing the C-terminal amidated beta-neurotoxin Ts1 with its isoform Ts1-G isolated from Tityus serrulatus venom."
    Coelho V.A., Cremonez C.M., Anjolette F.A., Aguiar J.F., Varanda W.A., Arantes E.C.
    Toxicon 83C:15-21(2014) [PubMed] [Europe PMC] [Abstract]
    Cited for: MUTAGENESIS OF CYS-81, MASS SPECTROMETRY.
  11. Cited for: NOMENCLATURE.
  12. "Crystal structure of neurotoxin Ts1 from Tityus serrulatus provides insights into the specificity and toxicity of scorpion toxins."
    Polikarpov I., Junior M.S.M., Marangoni S., Toyama M.H., Teplyakov A.
    J. Mol. Biol. 290:175-184(1999) [PubMed] [Europe PMC] [Abstract]
    Cited for: X-RAY CRYSTALLOGRAPHY (1.73 ANGSTROMS) OF 21-81.
  13. "Structural analysis of Tityus serrulatus Ts1 neurotoxin at atomic resolution: insights into interactions with Na+ channels."
    Pinheiro C.B., Marangoni S., Toyama M.H., Polikarpov I.
    Acta Crystallogr. D 59:405-415(2003) [PubMed] [Europe PMC] [Abstract]
    Cited for: X-RAY CRYSTALLOGRAPHY (1.16 ANGSTROMS) OF 21-81, DISULFIDE BONDS.
  14. "Identification and phylogenetic analysis of Tityus pachyurus and Tityus obscurus novel putative Na+-channel scorpion toxins."
    Guerrero-Vargas J.A., Mourao C.B., Quintero-Hernandez V., Possani L.D., Schwartz E.F.
    PLoS ONE 7:E30478-E30478(2012) [PubMed] [Europe PMC] [Abstract]
    Cited for: NOMENCLATURE.

Entry informationi

Entry nameiSCX1_TITSE
AccessioniPrimary (citable) accession number: P15226
Entry historyi
Integrated into UniProtKB/Swiss-Prot: April 1, 1990
Last sequence update: July 1, 1993
Last modified: May 14, 2014
This is version 96 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programAnimal Toxin Annotation Program

Miscellaneousi

Miscellaneous

Is the main neurotoxin of the venom of T.serrulatus.

Caution

The "mutant" Ts1-G was isolated from the venom before the last step maturation (i.e. amidation) thanks to a right purification technique (1 Publication). It is indicated here as a mutant, since it does not correspond to the mature toxin.

Keywords - Technical termi

3D-structure, Direct protein sequencing

Documents

  1. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

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