Skip Header

You are using a version of browser that may not display all the features of this website. Please consider upgrading your browser.
Basket 0
(max 400 entries)x

Your basket is currently empty.

Select item(s) and click on "Add to basket" to create your own collection here
(400 entries max)

P15226

- SCX1_TITSE

UniProt

P15226 - SCX1_TITSE

Protein

Beta-mammal/insect toxin Ts1

Gene
N/A
Organism
Tityus serrulatus (Brazilian scorpion)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at protein leveli
  1. Functioni

    Beta toxins bind voltage-independently at site-4 of sodium channels (Nav) and shift the voltage of activation toward more negative potentials thereby affecting sodium channel activation and promoting spontaneous and repetitive firing. In addition, it stimulates the release of NO, IL-6 and TNF-alpha in J774.1 cells (PubMed:21549737). This toxin is active against both mammals and insects.1 Publication

    GO - Molecular functioni

    1. ion channel inhibitor activity Source: InterPro

    GO - Biological processi

    1. defense response Source: InterPro

    Keywords - Molecular functioni

    Ion channel impairing toxin, Neurotoxin, Toxin, Voltage-gated sodium channel impairing toxin

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Beta-mammal/insect toxin Ts1
    Alternative name(s):
    PT-Mice-Ins-beta NaTx6.1
    Tityustoxin VII
    Short name:
    Ts VII
    Short name:
    Ts7
    Short name:
    TsTX-VII
    Toxin II-11
    Toxin III-10
    Toxin T2-IV
    Toxin gamma
    TsTX-I
    OrganismiTityus serrulatus (Brazilian scorpion)
    Taxonomic identifieri6887 [NCBI]
    Taxonomic lineageiEukaryotaMetazoaEcdysozoaArthropodaChelicerataArachnidaScorpionesButhidaButhoideaButhidaeTityus

    Subcellular locationi

    GO - Cellular componenti

    1. extracellular region Source: UniProtKB-SubCell

    Keywords - Cellular componenti

    Secreted

    Pathology & Biotechi

    Toxic dosei

    LD50 is 826 +/- 156 µg/kg by intravenous injection into mice.1 Publication
    LD50 is 11 +/- 9 µg/kg by intracistenal injection into mice.1 Publication
    LD50 is 0.6 ng, intracerebroventricular.1 Publication

    Mutagenesis

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Mutagenesisi81 – 811C → CG: Decrease of affinity of this non amidated toxin (Ts1-G) for sodium channel. 1 Publication

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Signal peptidei1 – 20204 PublicationsAdd
    BLAST
    Chaini21 – 8161Beta-mammal/insect toxin Ts1PRO_0000035305Add
    BLAST

    Amino acid modifications

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Disulfide bondi31 ↔ 811 Publication
    Disulfide bondi35 ↔ 571 Publication
    Disulfide bondi43 ↔ 621 Publication
    Disulfide bondi47 ↔ 641 Publication
    Modified residuei81 – 811Cysteine amide

    Keywords - PTMi

    Amidation, Disulfide bond

    Expressioni

    Tissue specificityi

    Expressed by the venom gland.

    Structurei

    Secondary structure

    1
    84
    Legend: HelixTurnBeta strand
    Show more details
    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Helixi42 – 498
    Beta strandi53 – 586
    Beta strandi61 – 666
    Turni76 – 783

    3D structure databases

    Select the link destinations:
    PDBe
    RCSB PDB
    PDBj
    Links Updated
    EntryMethodResolution (Å)ChainPositionsPDBsum
    1B7DX-ray1.73A21-81[»]
    1NPIX-ray1.16A21-81[»]
    ProteinModelPortaliP15226.
    SMRiP15226. Positions 21-81.
    ModBaseiSearch...
    MobiDBiSearch...

    Miscellaneous databases

    EvolutionaryTraceiP15226.

    Family & Domainsi

    Sequence similaritiesi

    Keywords - Domaini

    Signal

    Family and domain databases

    Gene3Di3.30.30.10. 1 hit.
    InterProiIPR003614. Scorpion_toxin-like.
    IPR018218. Scorpion_toxinL.
    IPR002061. Scorpion_toxinL/defensin.
    [Graphical view]
    PfamiPF00537. Toxin_3. 1 hit.
    [Graphical view]
    PRINTSiPR00285. SCORPNTOXIN.
    SMARTiSM00505. Knot1. 1 hit.
    [Graphical view]
    SUPFAMiSSF57095. SSF57095. 1 hit.

    Sequencei

    Sequence statusi: Complete.

    Sequence processingi: The displayed sequence is further processed into a mature form.

    P15226-1 [UniParc]FASTAAdd to Basket

    « Hide

    MKGMILFISC LLLIGIVVEC KEGYLMDHEG CKLSCFIRPS GYCGRECGIK   50
    KGSSGYCAWP ACYCYGLPNW VKVWDRATNK CGKK 84
    Length:84
    Mass (Da):9,382
    Last modified:July 1, 1993 - v2
    Checksum:i430F3FCA74823E77
    GO

    Mass spectrometryi

    Molecular mass is 6879.4 Da from positions 21 - 81. Determined by MALDI. 1 Publication

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    X66256 mRNA. Translation: CAA46982.1.
    S66941 Genomic DNA. Translation: AAB29128.1.
    PIRiS21158.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    X66256 mRNA. Translation: CAA46982.1 .
    S66941 Genomic DNA. Translation: AAB29128.1 .
    PIRi S21158.

    3D structure databases

    Select the link destinations:
    PDBe
    RCSB PDB
    PDBj
    Links Updated
    Entry Method Resolution (Å) Chain Positions PDBsum
    1B7D X-ray 1.73 A 21-81 [» ]
    1NPI X-ray 1.16 A 21-81 [» ]
    ProteinModelPortali P15226.
    SMRi P15226. Positions 21-81.
    ModBasei Search...
    MobiDBi Search...

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Miscellaneous databases

    EvolutionaryTracei P15226.

    Family and domain databases

    Gene3Di 3.30.30.10. 1 hit.
    InterProi IPR003614. Scorpion_toxin-like.
    IPR018218. Scorpion_toxinL.
    IPR002061. Scorpion_toxinL/defensin.
    [Graphical view ]
    Pfami PF00537. Toxin_3. 1 hit.
    [Graphical view ]
    PRINTSi PR00285. SCORPNTOXIN.
    SMARTi SM00505. Knot1. 1 hit.
    [Graphical view ]
    SUPFAMi SSF57095. SSF57095. 1 hit.
    ProtoNeti Search...

    Publicationsi

    1. "Molecular cloning and nucleotide sequence analysis of a cDNA encoding the main beta-neurotoxin from the venom of the South American scorpion Tityus serrulatus."
      Martin-Eauclaire M.-F., Ceard B., Ribeiro A.M., Diniz C.R., Rochat H., Bougis P.E.
      FEBS Lett. 302:220-222(1992) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [MRNA].
      Tissue: Venom gland.
    2. "The genomic region encoding toxin gamma from the scorpion Tityus serrulatus contains an intron."
      Becerril B., Corona M., Mejia M.C., Martin B.M., Lucas S., Bolivar F., Possani L.D.
      FEBS Lett. 335:6-8(1993) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
    3. "Amino acid sequence of toxin VII, a beta-toxin from the venom of the scorpion Tityus serrulatus."
      Bechis G., Sampieri F., Yuan P.-M., Brando T., Martin M.-F., Diniz C.R., Rochat H.
      Biochem. Biophys. Res. Commun. 122:1146-1153(1984) [PubMed] [Europe PMC] [Abstract]
      Cited for: PROTEIN SEQUENCE OF 21-81.
      Tissue: Venom.
    4. "Discharge effect on pancreatic exocrine secretion produced by toxins purified from Tityus serrulatus scorpion venom."
      Possani L.D., Martin B.M., Fletcher M.D., Fletcher P.L. Jr.
      J. Biol. Chem. 266:3178-3185(1991) [PubMed] [Europe PMC] [Abstract]
      Cited for: PROTEIN SEQUENCE OF 21-81.
      Tissue: Venom.
    5. "Purification and chemical characterization of the major toxins from the venom of the Brazilian scorpion Titus serrulatus Lutz and Mello."
      Possani L.D., Martin B.M., Mochca-Morales J., Svendsen I.
      Carlsberg Res. Commun. 46:195-205(1981)
      Cited for: PROTEIN SEQUENCE OF 21-62.
      Tissue: Venom.
    6. "Further characterization of toxins T1IV (TsTX-III) and T2IV from Tityus serrulatus scorpion venom."
      Sampaio S.V., Arantes E.C., Prado W.A., Riccioppo Neto F., Giglio J.R.
      Toxicon 29:663-672(1991) [PubMed] [Europe PMC] [Abstract]
      Cited for: PROTEIN SEQUENCE OF 21-46.
      Tissue: Venom.
    7. "A simplified procedure for the fractionation of Tityus serrulatus venom: isolation and partial characterization of TsTX-IV, a new neurotoxin."
      Arantes E.C., Prado W.A., Sampaio S.V., Giglio J.R.
      Toxicon 27:907-916(1989) [PubMed] [Europe PMC] [Abstract]
      Cited for: AMINO ACID COMPOSITION, LETHAL DOSE.
      Tissue: Venom.
    8. "Tityus serrulatus venom and toxins Ts1, Ts2 and Ts6 induce macrophage activation and production of immune mediators."
      Zoccal K.F., Bitencourt C.S., Secatto A., Sorgi C.A., Bordon K.C., Sampaio S.V., Arantes E.C., Faccioli L.H.
      Toxicon 57:1101-1108(2011) [PubMed] [Europe PMC] [Abstract]
      Cited for: FUNCTION.
    9. "Role of lysine and tryptophan residues in the biological activity of toxin VII (Ts gamma) from the scorpion Tityus serrulatus."
      Hassani O., Mansuelle P., Cestele S., Bourdeaux M., Rochat H., Sampieri F.
      Eur. J. Biochem. 260:76-86(1999) [PubMed] [Europe PMC] [Abstract]
      Cited for: IMPORTANCE OF LYS-12; TRP-39 AND TRP-54.
    10. "Functional and structural study comparing the C-terminal amidated beta-neurotoxin Ts1 with its isoform Ts1-G isolated from Tityus serrulatus venom."
      Coelho V.A., Cremonez C.M., Anjolette F.A., Aguiar J.F., Varanda W.A., Arantes E.C.
      Toxicon 83C:15-21(2014) [PubMed] [Europe PMC] [Abstract]
      Cited for: MUTAGENESIS OF CYS-81, MASS SPECTROMETRY.
    11. Cited for: NOMENCLATURE.
    12. "Crystal structure of neurotoxin Ts1 from Tityus serrulatus provides insights into the specificity and toxicity of scorpion toxins."
      Polikarpov I., Junior M.S.M., Marangoni S., Toyama M.H., Teplyakov A.
      J. Mol. Biol. 290:175-184(1999) [PubMed] [Europe PMC] [Abstract]
      Cited for: X-RAY CRYSTALLOGRAPHY (1.73 ANGSTROMS) OF 21-81.
    13. "Structural analysis of Tityus serrulatus Ts1 neurotoxin at atomic resolution: insights into interactions with Na+ channels."
      Pinheiro C.B., Marangoni S., Toyama M.H., Polikarpov I.
      Acta Crystallogr. D 59:405-415(2003) [PubMed] [Europe PMC] [Abstract]
      Cited for: X-RAY CRYSTALLOGRAPHY (1.16 ANGSTROMS) OF 21-81, DISULFIDE BONDS.
    14. "Identification and phylogenetic analysis of Tityus pachyurus and Tityus obscurus novel putative Na+-channel scorpion toxins."
      Guerrero-Vargas J.A., Mourao C.B., Quintero-Hernandez V., Possani L.D., Schwartz E.F.
      PLoS ONE 7:E30478-E30478(2012) [PubMed] [Europe PMC] [Abstract]
      Cited for: NOMENCLATURE.

    Entry informationi

    Entry nameiSCX1_TITSE
    AccessioniPrimary (citable) accession number: P15226
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: April 1, 1990
    Last sequence update: July 1, 1993
    Last modified: October 1, 2014
    This is version 97 of the entry and version 2 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programAnimal Toxin Annotation Program

    Miscellaneousi

    Miscellaneous

    Is the main neurotoxin of the venom of T.serrulatus.

    Caution

    The "mutant" Ts1-G was isolated from the venom before the last step maturation (i.e. amidation) thanks to a right purification technique (PubMed:24560880). It is indicated here as a mutant, since it does not correspond to the mature toxin.1 Publication

    Keywords - Technical termi

    3D-structure, Direct protein sequencing

    Documents

    1. PDB cross-references
      Index of Protein Data Bank (PDB) cross-references
    2. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3