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Protein

Insectotoxin-I5A

Gene
N/A
Organism
Mesobuthus eupeus (Lesser Asian scorpion) (Buthus eupeus)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Experimental evidence at protein leveli

Functioni

Toxin with unknown function in healthy organisms. On glioma cells, interacts with chloride channels (probably ClC-3/CLCN3) and MMP2 at the surface of glioma cells. This complex is then internalized via caveolae, thus inhibiting the chloride channels necessary for cell shrinkage and tumor propagation (By similarity).By similarity

GO - Biological processi

Keywordsi

Molecular functionChloride channel impairing toxin, Ion channel impairing toxin, Neurotoxin, Toxin, Voltage-gated chloride channel impairing toxin

Names & Taxonomyi

Protein namesi
Recommended name:
Insectotoxin-I5A
Short name:
BeIT5A
OrganismiMesobuthus eupeus (Lesser Asian scorpion) (Buthus eupeus)
Taxonomic identifieri34648 [NCBI]
Taxonomic lineageiEukaryotaMetazoaEcdysozoaArthropodaChelicerataArachnidaScorpionesButhidaButhoideaButhidaeMesobuthus

Subcellular locationi

GO - Cellular componenti

Keywords - Cellular componenti

Secreted

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
PeptideiPRO_00000449391 – 35Insectotoxin-I5AAdd BLAST35

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Disulfide bondi2 ↔ 19
Disulfide bondi5 ↔ 26
Disulfide bondi16 ↔ 31
Disulfide bondi20 ↔ 33
Modified residuei35Arginine amide1 Publication1

Keywords - PTMi

Amidation, Disulfide bond

Expressioni

Tissue specificityi

Expressed by the venom gland.

Structurei

Secondary structure

135
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details
Feature keyPosition(s)DescriptionActionsGraphical viewLength
Helixi12 – 19Combined sources8
Turni20 – 22Combined sources3
Beta strandi24 – 27Combined sources4
Beta strandi30 – 33Combined sources4

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
1SISNMR-A1-35[»]
ProteinModelPortaliP15222.
SMRiP15222.
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiP15222.

Family & Domainsi

Domaini

The presence of a 'disulfide through disulfide knot' structurally defines this protein as a knottin.

Sequence similaritiesi

Belongs to the short scorpion toxin superfamily. Chloride channel inhibitor family.PROSITE-ProRule annotation

Keywords - Domaini

Knottin

Family and domain databases

InterProiView protein in InterPro
IPR003614. Scorpion_toxin-like.
IPR007958. Scorpion_toxinS_Cl_inh.
PfamiView protein in Pfam
PF05294. Toxin_5. 1 hit.
SUPFAMiSSF57095. SSF57095. 1 hit.
PROSITEiView protein in PROSITE
PS51200. SHORT_SCORPION_CHLORIDE. 1 hit.

Sequencei

Sequence statusi: Complete.

P15222-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30 
MCMPCFTTDP NMAKKCRDCC GGNGKCFGPQ CLCNR
Length:35
Mass (Da):3,835
Last modified:October 1, 1994 - v2
Checksum:i47D269810B64318F
GO

Sequence databases

PIRiJN0361.

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.

Entry informationi

Entry nameiCTX5A_MESEU
AccessioniPrimary (citable) accession number: P15222
Entry historyiIntegrated into UniProtKB/Swiss-Prot: April 1, 1990
Last sequence update: October 1, 1994
Last modified: March 15, 2017
This is version 80 of the entry and version 2 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programAnimal Toxin Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Direct protein sequencing

Documents

  1. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  2. SIMILARITY comments
    Index of protein domains and families