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Reviewed, UniProtKB/Swiss-Prot P15209 (NTRK2_MOUSE)

Last modified March 2, 2010. Version 123. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (3) | Third-party data | Customize display text xml rdf/xml gff fasta
Names and origin · Protein attributes · General annotation (Comments) · Ontologies · Alternative products · Sequence annotation (Features) · Sequences · References · Cross-references · Entry information · Relevant documents

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Names and origin

Protein namesRecommended name:
BDNF/NT-3 growth factors receptor
EC=2.7.10.1
Alternative name(s):
Neurotrophic tyrosine kinase receptor type 2
TrkB tyrosine kinase
GP145-TrkB/GP95-TrkB
Short name=Trk-B
Gene names
Name:Ntrk2
Synonyms:Trkb
OrganismMus musculus (Mouse)
Taxonomic identifier10090 [NCBI]
Taxonomic lineageEukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Glires › Rodentia › Sciurognathi › Muroidea › Muridae › Murinae › Mus

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Protein attributes

Sequence length821 AA.
Sequence statusComplete.
Sequence processingThe displayed sequence is further processed into a mature form.
Protein existenceEvidence at protein level.

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General annotation (Comments)

Function

Receptor for brain-derived neurotrophic factor (BDNF), neurotrophin-3 and neurotrophin-4/5 but not nerve growth factor (NGF). Involved in the development and/or maintenance of the nervous system. This is a tyrosine-protein kinase receptor. Known substrates for the TRK receptors are SHC1, PI-3 kinase, and PLC-gamma-1. Ref.6

Catalytic activity

ATP + a [protein]-L-tyrosine = ADP + a [protein]-L-tyrosine phosphate.

Subunit structure

Exists in a dynamic equilibrium between monomeric (low affinity) and dimeric (high affinity) structures. Binds SH2B2. Interacts with SQSTM1 and KIDINS220 By similarity.

Subcellular location

Membrane; Single-pass type I membrane protein.

Tissue specificity

The different forms are differentially expressed in various cell types.

Post-translational modification

Ligand-mediated auto-phosphorylation.

Sequence similarities

Belongs to the protein kinase superfamily. Tyr protein kinase family. Insulin receptor subfamily.

Contains 2 Ig-like C2-type (immunoglobulin-like) domains.

Contains 2 LRR (leucine-rich) repeats.

Contains 1 protein kinase domain.

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Alternative products

This entry describes 4 isoforms produced by alternative splicing. [Align] [Select]

Note: Additional isoforms seem to exist.
Isoform GP145-TRKB (identifier: P15209-1)

Also known as: L3;

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.
Isoform GP95-TRKB (identifier: P15209-2)

Also known as: T1;

The sequence of this isoform differs from the canonical sequence as follows:
     466-476: PASVISNDDDS → FVLFHKIPLDG
     477-821: Missing.
Isoform L1 (identifier: P15209-3)

The sequence of this isoform differs from the canonical sequence as follows:
     72-120: Missing.
Isoform L10 (identifier: P15209-4)

The sequence of this isoform differs from the canonical sequence as follows:
     71-71: I → M
     72-143: Missing.

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Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Signal peptide1 – 3131
Chain32 – 821790BDNF/NT-3 growth factors receptor
PRO_0000016728

Regions

Topological domain32 – 429398Extracellular Potential
Transmembrane430 – 45324 Potential
Topological domain454 – 821368Cytoplasmic Potential
Repeat72 – 9322LRR 1
Repeat96 – 11722LRR 2
Domain197 – 28286Ig-like C2-type 1
Domain301 – 36565Ig-like C2-type 2
Domain537 – 806270Protein kinase
Nucleotide binding543 – 5519ATP By similarity

Sites

Active site6751Proton acceptor By similarity
Binding site5711ATP By similarity
Site5151Interaction with SHC1 By similarity
Site8161Interaction with PLC-gamma-1 By similarity

Amino acid modifications

Modified residue5151Phosphotyrosine; by autocatalysis By similarity
Modified residue7011Phosphotyrosine; by autocatalysis By similarity
Modified residue7051Phosphotyrosine; by autocatalysis By similarity
Modified residue7061Phosphotyrosine; by autocatalysis By similarity
Modified residue8161Phosphotyrosine; by autocatalysis By similarity
Glycosylation671N-linked (GlcNAc...) Potential
Glycosylation951N-linked (GlcNAc...) Potential
Glycosylation1211N-linked (GlcNAc...) Potential
Glycosylation1781N-linked (GlcNAc...) Potential
Glycosylation2051N-linked (GlcNAc...) Potential
Glycosylation2411N-linked (GlcNAc...) Potential
Glycosylation2541N-linked (GlcNAc...) Potential
Glycosylation2801N-linked (GlcNAc...) Potential
Glycosylation3251N-linked (GlcNAc...) Potential
Glycosylation3381N-linked (GlcNAc...) Potential
Glycosylation4111N-linked (GlcNAc...) Potential
Disulfide bond32 ↔ 38 By similarity
Disulfide bond36 ↔ 45 By similarity
Disulfide bond152 ↔ 176 By similarity
Disulfide bond154 ↔ 194 By similarity
Disulfide bond218 ↔ 266 By similarity
Disulfide bond302 ↔ 345 By similarity

Natural variations

Alternative sequence711I → M in isoform L10.
VSP_002905
Alternative sequence72 – 14372Missing in isoform L10.
VSP_002906
Alternative sequence72 – 12049Missing in isoform L1.
VSP_002907
Alternative sequence466 – 47611PASVISNDDDS → FVLFHKIPLDG in isoform GP95-TRKB.
VSP_002908
Alternative sequence477 – 821345Missing in isoform GP95-TRKB.
VSP_002909

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Sequences

Sequence LengthMass (Da)Tools
Isoform GP145-TRKB (L3) [UniParc].

Last modified April 1, 1990. Version 1.
Checksum: 50E08D5FF86D8F30

FASTA82192,133
        10         20         30         40         50         60 
MSPWLKWHGP AMARLWGLCL LVLGFWRASL ACPTSCKCSS ARIWCTEPSP GIVAFPRLEP 

        70         80         90        100        110        120 
NSVDPENITE ILIANQKRLE IINEDDVEAY VGLRNLTIVD SGLKFVAYKA FLKNSNLRHI 

       130        140        150        160        170        180 
NFTRNKLTSL SRRHFRHLDL SDLILTGNPF TCSCDIMWLK TLQETKSSPD TQDLYCLNES 

       190        200        210        220        230        240 
SKNMPLANLQ IPNCGLPSAR LAAPNLTVEE GKSVTLSCSV GGDPLPTLYW DVGNLVSKHM 

       250        260        270        280        290        300 
NETSHTQGSL RITNISSDDS GKQISCVAEN LVGEDQDSVN LTVHFAPTIT FLESPTSDHH 

       310        320        330        340        350        360 
WCIPFTVRGN PKPALQWFYN GAILNESKYI CTKIHVTNHT EYHGCLQLDN PTHMNNGDYT 

       370        380        390        400        410        420 
LMAKNEYGKD ERQISAHFMG RPGVDYETNP NYPEVLYEDW TTPTDIGDTT NKSNEIPSTD 

       430        440        450        460        470        480 
VADQSNREHL SVYAVVVIAS VVGFCLLVML LLLKLARHSK FGMKGPASVI SNDDDSASPL 

       490        500        510        520        530        540 
HHISNGSNTP SSSEGGPDAV IIGMTKIPVI ENPQYFGITN SQLKPDTFVQ HIKRHNIVLK 

       550        560        570        580        590        600 
RELGEGAFGK VFLAECYNLC PEQDKILVAV KTLKDASDNA RKDFHREAEL LTNLQHEHIV 

       610        620        630        640        650        660 
KFYGVCVEGD PLIMVFEYMK HGDLNKFLRA HGPDAVLMAE GNPPTELTQS QMLHIAQQIA 

       670        680        690        700        710        720 
AGMVYLASQH FVHRDLATRN CLVGENLLVK IGDFGMSRDV YSTDYYRVGG HTMLPIRWMP 

       730        740        750        760        770        780 
PESIMYRKFT TESDVWSLGV VLWEIFTYGK QPWYQLSNNE VIECITQGRV LQRPRTCPQE 

       790        800        810        820 
VYELMLGCWQ REPHTRKNIK SIHTLLQNLA KASPVYLDIL G

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Isoform GP95-TRKB (T1).

Checksum: 20A8B375ED397ACE
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FASTA47653,186
Isoform L1.

Checksum: E3E800582F9D3FB6
Show »

FASTA77286,535
Isoform L10.

Checksum: 791E6861C97AA53F
Show »

FASTA74983,744

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References

« Hide 'large scale' references
[1]"trkB, a novel tyrosine protein kinase receptor expressed during mouse neural development."
Klein R., Parada L.F., Coulier F., Barbacid M.
EMBO J. 8:3701-3709(1989) [PubMed: 2555172] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM GP145-TRKB).
Tissue: Brain.
[2]"The trkB tyrosine protein kinase gene codes for a second neurogenic receptor that lacks the catalytic kinase domain."
Klein R., Conway D., Parada L.F., Barbacid M.
Cell 61:647-656(1990) [PubMed: 2160854] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORMS GP145-TRKB AND GP95-TRKB).
Tissue: Brain.
[3]"The transcriptional landscape of the mammalian genome."
Carninci P., Kasukawa T., Katayama S., Gough J., Frith M.C., Maeda N., Oyama R., Ravasi T., Lenhard B., Wells C., Kodzius R., Shimokawa K., Bajic V.B., Brenner S.E., Batalov S., Forrest A.R., Zavolan M., Davis M.J. expand/collapse author list , Wilming L.G., Aidinis V., Allen J.E., Ambesi-Impiombato A., Apweiler R., Aturaliya R.N., Bailey T.L., Bansal M., Baxter L., Beisel K.W., Bersano T., Bono H., Chalk A.M., Chiu K.P., Choudhary V., Christoffels A., Clutterbuck D.R., Crowe M.L., Dalla E., Dalrymple B.P., de Bono B., Della Gatta G., di Bernardo D., Down T., Engstrom P., Fagiolini M., Faulkner G., Fletcher C.F., Fukushima T., Furuno M., Futaki S., Gariboldi M., Georgii-Hemming P., Gingeras T.R., Gojobori T., Green R.E., Gustincich S., Harbers M., Hayashi Y., Hensch T.K., Hirokawa N., Hill D., Huminiecki L., Iacono M., Ikeo K., Iwama A., Ishikawa T., Jakt M., Kanapin A., Katoh M., Kawasawa Y., Kelso J., Kitamura H., Kitano H., Kollias G., Krishnan S.P., Kruger A., Kummerfeld S.K., Kurochkin I.V., Lareau L.F., Lazarevic D., Lipovich L., Liu J., Liuni S., McWilliam S., Madan Babu M., Madera M., Marchionni L., Matsuda H., Matsuzawa S., Miki H., Mignone F., Miyake S., Morris K., Mottagui-Tabar S., Mulder N., Nakano N., Nakauchi H., Ng P., Nilsson R., Nishiguchi S., Nishikawa S., Nori F., Ohara O., Okazaki Y., Orlando V., Pang K.C., Pavan W.J., Pavesi G., Pesole G., Petrovsky N., Piazza S., Reed J., Reid J.F., Ring B.Z., Ringwald M., Rost B., Ruan Y., Salzberg S.L., Sandelin A., Schneider C., Schoenbach C., Sekiguchi K., Semple C.A., Seno S., Sessa L., Sheng Y., Shibata Y., Shimada H., Shimada K., Silva D., Sinclair B., Sperling S., Stupka E., Sugiura K., Sultana R., Takenaka Y., Taki K., Tammoja K., Tan S.L., Tang S., Taylor M.S., Tegner J., Teichmann S.A., Ueda H.R., van Nimwegen E., Verardo R., Wei C.L., Yagi K., Yamanishi H., Zabarovsky E., Zhu S., Zimmer A., Hide W., Bult C., Grimmond S.M., Teasdale R.D., Liu E.T., Brusic V., Quackenbush J., Wahlestedt C., Mattick J.S., Hume D.A., Kai C., Sasaki D., Tomaru Y., Fukuda S., Kanamori-Katayama M., Suzuki M., Aoki J., Arakawa T., Iida J., Imamura K., Itoh M., Kato T., Kawaji H., Kawagashira N., Kawashima T., Kojima M., Kondo S., Konno H., Nakano K., Ninomiya N., Nishio T., Okada M., Plessy C., Shibata K., Shiraki T., Suzuki S., Tagami M., Waki K., Watahiki A., Okamura-Oho Y., Suzuki H., Kawai J., Hayashizaki Y.
Science 309:1559-1563(2005) [PubMed: 16141072] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORMS GP145-TRKB AND GP95-TRKB).
Strain: C57BL/6J and NOD.
Tissue: Thymus.
[4]"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
The MGC Project Team
Genome Res. 14:2121-2127(2004) [PubMed: 15489334] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM GP95-TRKB).
Strain: C57BL/6.
Tissue: Brain.
[5]"TrkB variants with deletions in the leucine-rich motifs of the extracellular domain."
Ninkina N., Grashchuck M., Buchman V.L., Davies A.M.
J. Biol. Chem. 272:13019-13025(1997) [PubMed: 9148911] [Abstract]
Cited for: PARTIAL NUCLEOTIDE SEQUENCE (ISOFORMS L1 AND L10).
Tissue: Trigeminal ganglion.
[6]"The neurotrophic factors brain-derived neurotrophic factor and neurotrophin-3 are ligands for the trkB tyrosine kinase receptor."
Soppet D., Escandon E., Maragos J., Middlemas D.S., Reid S.W., Blair J., Burton L.E., Stanton B.R., Kaplan D.R., Hunter T., Nicolics K., Parada L.F.
Cell 65:895-903(1991) [PubMed: 1645620] [Abstract]
Cited for: FUNCTION.
[7]"Large-scale identification and evolution indexing of tyrosine phosphorylation sites from murine brain."
Ballif B.A., Carey G.R., Sunyaev S.R., Gygi S.P.
J. Proteome Res. 7:311-318(2008) [PubMed: 18034455] [Abstract]
Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT TYR-515, MASS SPECTROMETRY.
Tissue: Brain.
+Additional computationally mapped references.

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Cross-references

Sequence databases

EMBL
GenBank
DDBJ		M33385 mRNA. Translation: AAA40482.1.
X17647 mRNA. Translation: CAA35636.1.
AK018789 mRNA. Translation: BAB31412.1.
AK147391 mRNA. Translation: BAE27880.1.
AK160789 mRNA. Translation: BAE36012.1.
BC052014 mRNA. Translation: AAH52014.2.
IPIIPI00128360.
IPI00229333.
IPI00229334.
IPI00270098.
PIRA35104.
S06943.
RefSeqNP_001020245.1.
NP_032771.1.
UniGeneMm.130054

3D structure databases

SMRP15209. Positions 32-193, 90-284, 203-374, 283-385, 531-810.
ModBaseSearch...

Protein-protein interaction databases

DIPDIP-5722N.
IntActP15209. 1 interaction.
STRINGP15209.

PTM databases

PhosphoSiteP15209.

Proteomic databases

PRIDEP15209.

Genome annotation databases

EnsemblENSMUST00000078541; ENSMUSP00000077623; ENSMUSG00000055254; Mus musculus. [Genome view]
ENSMUST00000079828; ENSMUSP00000078757; ENSMUSG00000055254; Mus musculus. [Genome view]
ENSMUST00000109838; ENSMUSP00000105464; ENSMUSG00000055254; Mus musculus. [Genome view]
ENSMUST00000109839; ENSMUSP00000105465; ENSMUSG00000055254; Mus musculus. [Genome view]
ENSMUST00000109840; ENSMUSP00000105466; ENSMUSG00000055254; Mus musculus. [Genome view]
GeneID18212.
KEGGmmu:18212.
UCSCuc007qui.1. mouse.

Organism-specific databases

CTD18212.
MGIMGI:97384. Ntrk2.

Phylogenomic databases

eggNOGroNOG12558.
HOGENOMHBG402948.
HOVERGENHBG056735.
InParanoidP15209.
OMAKFVAYKA.
OrthoDBEOG9T4GDW.
PhylomeDBP15209.

Enzyme and pathway databases

BRENDA2.7.10.1. 244.

Gene expression databases

ArrayExpressP15209.
BgeeP15209.
CleanExMM_NTRK2.
GenevestigatorP15209.
GermOnlineENSMUSG00000055254. Mus musculus.

Family and domain databases

InterProIPR000483. Cys-rich_flank_reg_C.
IPR007110. Ig-like.
IPR013783. Ig-like_fold.
IPR003598. Ig_sub2.
IPR011009. Kinase-like_dom.
IPR000372. LRR-contain_N.
IPR000719. Prot_kinase_cat_dom.
IPR017441. Protein_kinase_ATP_BS.
IPR020455. Tyr_kin_neurotrophic_rcpt_2_N.
IPR020426. Tyr_kinase_neurotrophic_rcpt_N.
IPR020635. Tyr_Pkinase_cat_dom.
IPR020685. Tyr_prot_kinase.
IPR008266. Tyr_prot_kinase_AS.
IPR002011. Tyr_prot_kinase_rcpt_2_CS.
[Graphical view]
Gene3DG3DSA:2.60.40.10. Ig-like_fold. 1 hit.
PANTHERPTHR23256. Tyr_prot_kinase. 1 hit.
PfamPF01462. LRRNT. 1 hit.
[Graphical view]
PRINTSPR01939. NTKRECEPTOR.
PR01941. NTKRECEPTOR2.
SMARTSM00408. IGc2. 1 hit.
SM00082. LRRCT. 1 hit.
SM00013. LRRNT. 1 hit.
SM00219. TyrKc. 1 hit.
[Graphical view]
SUPFAMSSF56112. Kinase_like. 1 hit.
PROSITEPS50835. IG_LIKE. 1 hit.
PS00107. PROTEIN_KINASE_ATP. 1 hit.
PS50011. PROTEIN_KINASE_DOM. 1 hit.
PS00109. PROTEIN_KINASE_TYR. 1 hit.
PS00239. RECEPTOR_TYR_KIN_II. 1 hit.
[Graphical view]
ProtoNetSearch...

Other Resources

NextBio293608.
SOURCESearch...

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Entry information

Entry nameNTRK2_MOUSE
AccessionPrimary (citable) accession number: P15209
Secondary accession number(s): Q3TUF9, Q80WU0, Q91XJ9
Entry history
Integrated into UniProtKB/Swiss-Prot: April 1, 1990
Last sequence update: April 1, 1990
Last modified: March 2, 2010
This is version 123 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation projectHPI (Human Proteome Initiative)

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Relevant documents

MGD cross-references

Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot

Human and mouse protein kinases

Human and mouse protein kinases: classification and index

SIMILARITY comments

Index of protein domains and families

Names and origin · Protein attributes · General annotation (Comments) · Ontologies · Alternative products · Sequence annotation (Features) · Sequences · References · Cross-references · Entry information · Relevant documents