P15198 (PHOSP_RABVA) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Phosphoprotein
Short name=Protein P

Alternative name(s):
Protein M1

Gene names

Name:

P

Organism

Rabies virus (strain PM1503/AVO1) (RABV) [Complete proteome]

Taxonomic identifier

11293 [NCBI]

Taxonomic lineage

Viruses › ssRNA viruses › ssRNA negative-strand viruses › Mononegavirales › Rhabdoviridae › Lyssavirus › Rabies virus

Virus host

Homo sapiens (Human) [TaxID: 9606]
Mammalia [TaxID: 40674]

Protein attributes

Sequence length	297 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	Non catalytic polymerase cofactor and regulatory protein that plays a role in viral transcription and replication. Stabilizes the RNA polymerase L to the N-RNA template and binds the soluble protein N, preventing it from encapsidating non-genomic RNA. Also inhibits host IFN-alpha and IFN-beta signaling by binding and retaining phosphorylated STAT1 in the cytoplasm or by inhibiting the DNA binding of STAT1 in the nucleus. Might be involved, through interaction with host dynein, in intracellular microtubule-dependent virus transport of incoming virus from the synapse toward the cell body By similarity.
Subunit structure	Homotrimer when phosphorylated. This trimer is stabilized by binding to the L protein. Binds soluble protein N, and ribonucleocapsid. Interacts with host DYNLL1 and DYNLL2; this interaction may play a role in intracellular microtubule-dependent virus transport of incoming virus. Interacts with host STAT1, STAT2 and PML. Isoform P3 binds host PML By similarity.
Subcellular location	Phosphoprotein: Virion. Host cytoplasm By similarity. Isoform P2: Host cytoplasm By similarity. Isoform P3: Host nucleus By similarity. Isoform P4: Host nucleus By similarity. Isoform P5: Host nucleus By similarity.
Post-translational modification	Phosphorylated by host PKC and by an unknown kinase By similarity.
Sequence similarities	Belongs to the lyssavirus protein P family.

Ontologies

Keywords
Biological process	Cytoplasmic inwards viral transport Host-virus interaction Inhibition of host STAT1 by virus Inhibition of host STAT2 by virus Inhibition of host innate immune response by virus Inhibition of host interferon signaling pathway by virus Interferon antiviral system evasion Microtubular inwards viral transport Viral RNA replication Viral immunoevasion Virus entry into host cell
Cellular component	Host cytoplasm Host nucleus Virion
Coding sequence diversity	Alternative initiation
Molecular function	Chaperone
PTM	Phosphoprotein
Technical term	Complete proteome
Gene Ontology (GO)
Biological_process	suppression by virus of host STAT1 activity Inferred from electronic annotation. Source: UniProtKB-KW suppression by virus of host STAT2 activity Inferred from electronic annotation. Source: UniProtKB-KW suppression by virus of host type I interferon-mediated signaling pathway Inferred from electronic annotation. Source: UniProtKB-KW viral entry into host cell Inferred from electronic annotation. Source: UniProtKB-KW viral transcription Inferred from electronic annotation. Source: InterPro
Cellular_component	host cell cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell host cell nucleus Inferred from electronic annotation. Source: UniProtKB-SubCell virion Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular_function	RNA-directed RNA polymerase activity Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Alternative products

This entry describes 5 isoforms produced by alternative initiation. [Align] [Select]

Isoform P (identifier: P15198-1)

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

Isoform P2 (identifier: P15198-2)

The sequence of this isoform differs from the canonical sequence as follows:
     1-19: Missing.

Isoform P3 (identifier: P15198-3)

The sequence of this isoform differs from the canonical sequence as follows:
     1-52: Missing.

Isoform P4 (identifier: P15198-4)

The sequence of this isoform differs from the canonical sequence as follows:
     1-68: Missing.

Isoform P5 (identifier: P15198-5)

The sequence of this isoform differs from the canonical sequence as follows:
     1-82: Missing.

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 297	297	Phosphoprotein		PRO_0000222827
Regions
	Region	138 – 172	35	DYNLL1 and DYNLL2 binding By similarity
	Motif	49 – 58	10	Nuclear export signal By similarity
	Motif	211 – 214	4	Nuclear localization signal By similarity
Amino acid modifications
	Modified residue	63	1	Phosphoserine; by host By similarity
	Modified residue	64	1	Phosphoserine; by host By similarity
	Modified residue	162	1	Phosphoserine; by host PKC By similarity
	Modified residue	210	1	Phosphoserine; by host PKC By similarity
	Modified residue	271	1	Phosphoserine; by host PKC By similarity
Natural variations
	Alternative sequence	1 – 82	82	Missing in isoform P5.		VSP_026864
	Alternative sequence	1 – 68	68	Missing in isoform P4.		VSP_026865
	Alternative sequence	1 – 52	52	Missing in isoform P3.		VSP_026866
	Alternative sequence	1 – 19	19	Missing in isoform P2.		VSP_026867
	Natural variant	30	1	R → Q in strain: Isolate PM1503.
	Natural variant	36	1	D → Q in strain: Isolate PM1503.
	Natural variant	84	1	D → G in strain: Isolate PM1503.
	Natural variant	122	1	V → I in strain: Isolate PM1503.
	Natural variant	179	1	P → S in strain: Isolate PM1503.
	Natural variant	223	1	F → L in strain: Isolate PM1503.

Sequences

	Sequence		Length	Mass (Da)
	Isoform P [UniParc]. Last modified April 1, 1990. Version 1. Checksum: E935DEDBFFA3DB06	FASTA	297	33,603
	10 20 30 40 50 60 MSKIFVNPSA IRAGLADLEM AEETVDLINR NIEDNDAHLQ GEPIEVDNLP EDMKRLHLDD 70 80 90 100 110 120 EKSSNLGEMV RVGEGKYRED FQMDEGEDPN LLFQSYLDNV GVQIVRQMRS GERFLKIWSQ 130 140 150 160 170 180 TVEEIVSYVT VNFPNPPRRS SEDKSTQTTG RELKKETTSA FSQRESQPSK ARMVAQVAPG 190 200 210 220 230 240 PPALEWSATN EEDDLSVEAE IAHQIAESFS KKYKFPSRSS GIFLYNFEQL KMNLDDIVKE 250 260 270 280 290 AKNVPGVTRL AHDGSKIPLR CVLGWVALAN SKKFQLLVEA DKLSKIMQDD LNRYTSC
	Isoform P2 [UniParc]. Checksum: 4CA2838BA656511C	FASTA	278	31,588
	10 20 30 40 50 60 MAEETVDLIN RNIEDNDAHL QGEPIEVDNL PEDMKRLHLD DEKSSNLGEM VRVGEGKYRE 70 80 90 100 110 120 DFQMDEGEDP NLLFQSYLDN VGVQIVRQMR SGERFLKIWS QTVEEIVSYV TVNFPNPPRR 130 140 150 160 170 180 SSEDKSTQTT GRELKKETTS AFSQRESQPS KARMVAQVAP GPPALEWSAT NEEDDLSVEA 190 200 210 220 230 240 EIAHQIAESF SKKYKFPSRS SGIFLYNFEQ LKMNLDDIVK EAKNVPGVTR LAHDGSKIPL 250 260 270 RCVLGWVALA NSKKFQLLVE ADKLSKIMQD DLNRYTSC
	Isoform P3 [UniParc]. Checksum: C43E30CC191C3F10	FASTA	245	27,857
	10 20 30 40 50 60 MKRLHLDDEK SSNLGEMVRV GEGKYREDFQ MDEGEDPNLL FQSYLDNVGV QIVRQMRSGE 70 80 90 100 110 120 RFLKIWSQTV EEIVSYVTVN FPNPPRRSSE DKSTQTTGRE LKKETTSAFS QRESQPSKAR 130 140 150 160 170 180 MVAQVAPGPP ALEWSATNEE DDLSVEAEIA HQIAESFSKK YKFPSRSSGI FLYNFEQLKM 190 200 210 220 230 240 NLDDIVKEAK NVPGVTRLAH DGSKIPLRCV LGWVALANSK KFQLLVEADK LSKIMQDDLN RYTSC
	Isoform P4 [UniParc]. Checksum: 25D7A21F4A4DB726	FASTA	229	26,003
	10 20 30 40 50 60 MVRVGEGKYR EDFQMDEGED PNLLFQSYLD NVGVQIVRQM RSGERFLKIW SQTVEEIVSY 70 80 90 100 110 120 VTVNFPNPPR RSSEDKSTQT TGRELKKETT SAFSQRESQP SKARMVAQVA PGPPALEWSA 130 140 150 160 170 180 TNEEDDLSVE AEIAHQIAES FSKKYKFPSR SSGIFLYNFE QLKMNLDDIV KEAKNVPGVT 190 200 210 220 RLAHDGSKIP LRCVLGWVAL ANSKKFQLLV EADKLSKIMQ DDLNRYTSC
	Isoform P5 [UniParc]. Checksum: 48C26245875004D2	FASTA	215	24,307
	10 20 30 40 50 60 MDEGEDPNLL FQSYLDNVGV QIVRQMRSGE RFLKIWSQTV EEIVSYVTVN FPNPPRRSSE 70 80 90 100 110 120 DKSTQTTGRE LKKETTSAFS QRESQPSKAR MVAQVAPGPP ALEWSATNEE DDLSVEAEIA 130 140 150 160 170 180 HQIAESFSKK YKFPSRSSGI FLYNFEQLKM NLDDIVKEAK NVPGVTRLAH DGSKIPLRCV 190 200 210 LGWVALANSK KFQLLVEADK LSKIMQDDLN RYTSC

References

[1]	"Sequence of the 3386 3' nucleotides of the genome of the AVO1 strain rabies virus: structural similarities in the protein regions involved in transcription." Poch O., Tordo N., Keith G. Biochimie 70:1019-1029(1988) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC RNA].
[2]	"Characterization of rabies virus vaccine strains." Stallkamp I., Lopez-Yomayuza C.C., Thiel H.-J. Submitted (JUN-2005) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [GENOMIC RNA]. Strain: Isolate PM1503.

Cross-references

Sequence databases
EMBL GenBank DDBJ	X13357 Genomic RNA. Translation: CAA31734.1. DQ099525 Genomic RNA. Translation: AAZ07892.1.
PIR	MNVNAV. S07814.
3D structure databases
ProteinModelPortal	P15198.
SMR	P15198. Positions 186-296.
ModBase	Search...
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Family and domain databases
InterPro	IPR004259. PP_M1. [Graphical view]
Pfam	PF03012. PP_M1. 1 hit. [Graphical view]
ProDom	PD004155. PP_M1. 1 hit. [Graphical view] [Entries sharing at least one domain]
ProtoNet	Search...

Entry information

Entry name

PHOSP_RABVA

Accession

Primary (citable) accession number: P15198
Secondary accession number(s): Q4F902

Entry history

Integrated into UniProtKB/Swiss-Prot:	April 1, 1990
Last sequence update:	April 1, 1990
Last modified:	April 3, 2013
This is version 59 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Viral Protein Annotation Program