P15197 (NCAP_RABVA) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Nucleoprotein
Short name=NP

Alternative name(s):
Nucleocapsid protein
Short name=Protein N

Gene names

Name:

N

Organism

Rabies virus (strain PM1503/AVO1) (RABV) [Complete proteome]

Taxonomic identifier

11293 [NCBI]

Taxonomic lineage

Viruses › ssRNA viruses › ssRNA negative-strand viruses › Mononegavirales › Rhabdoviridae › Lyssavirus › Rabies virus

Virus host

Homo sapiens (Human) [TaxID: 9606]
Mammalia [TaxID: 40674]

Protein attributes

Sequence length	450 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	Encapsidates the genome in a ratio of one protein N per nine ribonucleotides, protecting it from nucleases. If expressed without protein P it binds non-specifically RNA and therefore can bind it's own mRNA. Interaction with protein P abolishes any non-specific RNA binding, and prevents phosphorylation. The soluble N-P complex encapsidates specifically the genomic RNA, with protein N protecting the genome like a pearl necklace. The encapsidated genomic RNA is termed the nucleocapsid (NC) and serves as template for viral transcription and replication. Protein N binds protein P in the NC through a different interaction, and can be phosphorylated. Subsequent viral replication is dependent on intracellular concentration of newly synthesized protein N. During replication, encapsidation by protein N is coupled to RNA synthesis and all replicative products are resistant to nucleases By similarity.
Subunit structure	Homomultimerizes to form the nucleocapsid. Binds to viral genomic RNA. In nucleocapsid, binds protein P and thereby positions the polymerase on the template. Protein P acts as a chaperone on free protein N to prevent it from aggregation before encapsidating genomic RNA By similarity.
Subcellular location	Virion. Host cytoplasm By similarity.
Post-translational modification	Phosphorylated by host CK2. Unphosphorylated protein N seems to have a better affinity for leader viral promoter encapsidation. Phosphorylation of protein N in ribonucleocapsid may stabilize the interaction with protein P, thereby playing an important role in viral transcription/replication By similarity.
Miscellaneous	Displays a superantigen activity in human and mouse, activating mostly V-beta-8 subtypes of T-cell receptor By similarity.
Sequence similarities	Belongs to the lyssavirus nucleocapsid protein family.

Ontologies

Keywords
Cellular component	Host cytoplasm Virion
Ligand	RNA-binding Viral nucleoprotein
Molecular function	Ribonucleoprotein Superantigen
PTM	Phosphoprotein
Technical term	Complete proteome
Gene Ontology (GO)
Cellular_component	host cell cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell ribonucleoprotein complex Inferred from electronic annotation. Source: UniProtKB-KW viral nucleocapsid Inferred from electronic annotation. Source: InterPro
Molecular_function	RNA binding Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 450	450	Nucleoprotein		PRO_0000222816
Amino acid modifications
	Modified residue	389	1	Phosphoserine; by host CK2 By similarity
Natural variations
	Natural variant	377	1	T → S in strain: Isolate AVO1.

Sequences

	Sequence		Length	Mass (Da)
	P15197 [UniParc]. Last modified July 10, 2007. Version 2. Checksum: 004FE0FC57ED8176	FASTA	450	50,748
	10 20 30 40 50 60 MDADKIVFKV NNQVVSLKPE IIVDQYEYKY PAIKDLKKPC ITLGKAPDLN KAYKSVLSGM 70 80 90 100 110 120 NAAKLDPDDV CSYLAAAMQF FEGTCPEDWT SYGILIARKG DRITPNSLVE IKRTDVEGNW 130 140 150 160 170 180 ALTGGMELTR DPTVSEHASL VGLLLSLYRL SKISGQNTGN YKTNIADRIE QIFETAPFVK 190 200 210 220 230 240 IVEHHTLMTT HKMCANWSTI PNFRFLAGTY DMFFSRIEHL YSAIRVGTVV TAYEDCSGLV 250 260 270 280 290 300 SFTGFIKQIN LTAREAILYF FHKNFEEEIR RMFEPGQETA VPHSYFIHFR SLGLSGKSPY 310 320 330 340 350 360 SSNAVGHVFN LIHFVGCYMG QVRSLNATVI AACAPHEMSV LGGYLGEEFF GKGTFERRFF 370 380 390 400 410 420 RDEKELQEYE AAELTKTDVA LADDGTVNSD DEDYFSGETR SPEAVYTRIM MNGGRLKRSH 430 440 450 IRRYVSVSSN HQARPNSFAE FLNKTYSNDS

References

[1]	"Sequence of the 3386 3' nucleotides of the genome of the AVO1 strain rabies virus: structural similarities in the protein regions involved in transcription." Poch O., Tordo N., Keith G. Biochimie 70:1019-1029(1988) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC RNA]. Strain: Isolate AVO1.
[2]	"Characterization of rabies virus vaccine strains." Stallkamp I., Lopez-Yomayuza C.C., Thiel H.-J. Submitted (JUN-2005) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [GENOMIC RNA]. Strain: Isolate PM1503.

Cross-references

Sequence databases
EMBL GenBank DDBJ	X13357 Genomic RNA. Translation: CAA31733.1. DQ099525 Genomic RNA. Translation: AAZ07891.1.
PIR	VHVNAV. S07813.
3D structure databases
ProteinModelPortal	P15197.
SMR	P15197. Positions 5-448.
ModBase	Search...
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Family and domain databases
Gene3D	1.10.3570.10. 1 hit. 1.10.3610.10. 1 hit.
InterPro	IPR000448. Rhabdo_ncapsid. IPR023331. Rhabdovirus_ncapsid_C. IPR023330. Rhabdovirus_ncapsid_N. [Graphical view]
Pfam	PF00945. Rhabdo_ncap. 1 hit. [Graphical view]
ProDom	PD002087. Rhabd_nucleocap. 1 hit. [Graphical view] [Entries sharing at least one domain]
SUPFAM	SSF140809. SSF140809. 1 hit.
ProtoNet	Search...

Entry information

Entry name

NCAP_RABVA

Accession

Primary (citable) accession number: P15197
Secondary accession number(s): Q4F903

Entry history

Integrated into UniProtKB/Swiss-Prot:	April 1, 1990
Last sequence update:	July 10, 2007
Last modified:	April 3, 2013
This is version 52 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Viral Protein Annotation Program