Skip Header

You are using a version of browser that may not display all the features of this website. Please consider upgrading your browser.
Protein

RNA-directed RNA polymerase

Gene

ORF1

Organism
Cucumber necrosis virus (CNV)
Status
Reviewed-Annotation score: Annotation score: 2 out of 5-Protein inferred from homologyi

Functioni

RNA-dependent RNA polymerase that plays an essential role in the virus replication.Curated

Catalytic activityi

Nucleoside triphosphate + RNA(n) = diphosphate + RNA(n+1).PROSITE-ProRule annotation

GO - Molecular functioni

  1. nucleotide binding Source: UniProtKB-KW
  2. RNA binding Source: InterPro
  3. RNA-directed RNA polymerase activity Source: UniProtKB-KW

GO - Biological processi

  1. viral RNA genome replication Source: InterPro
Complete GO annotation...

Keywords - Molecular functioni

Nucleotidyltransferase, RNA-directed RNA polymerase, Transferase

Keywords - Biological processi

Viral RNA replication

Keywords - Ligandi

Nucleotide-binding

Names & Taxonomyi

Protein namesi
Recommended name:
RNA-directed RNA polymerase (EC:2.7.7.48)
Alternative name(s):
Protein p92
Cleaved into the following chain:
Gene namesi
ORF Names:ORF1
OrganismiCucumber necrosis virus (CNV)
Taxonomic identifieri12143 [NCBI]
Taxonomic lineageiVirusesssRNA positive-strand viruses, no DNA stageTombusviridaeTombusvirus
Virus hostiCucumis sativus (Cucumber) [TaxID: 3659]
ProteomesiUP000008565 Componenti: Genome

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 818818RNA-directed RNA polymerasePRO_0000041332Add
BLAST
Chaini1 – 296296Protein p33PRO_0000041333Add
BLAST

Structurei

3D structure databases

ProteinModelPortaliP15187.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini524 – 641118RdRp catalyticPROSITE-ProRule annotationAdd
BLAST

Sequence similaritiesi

Contains 1 RdRp catalytic domain.PROSITE-ProRule annotation

Family and domain databases

InterProiIPR007094. RNA-dir_pol_PSvirus.
IPR002166. RNA_pol_HCV.
IPR013707. Tombusvirus_p33.
[Graphical view]
PfamiPF00998. RdRP_3. 1 hit.
PF08500. Tombus_P33. 1 hit.
[Graphical view]
PROSITEiPS50507. RDRP_SSRNA_POS. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

P15187-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MDTIKRMLWP KKEIFVGTFA TGVERDTSVD IFQLVCRVVL RYMRTGKIEN
60 70 80 90 100
NTDSLGNFIV ELLKTDCAAK WEWFMKRRRV GDYAKSLAIA SIPVIPLLSY
110 120 130 140 150
ATMKKTVALR AFGNELSFNI RVPRPSVPKK GLLLRLAAGL ALAPICALAM
160 170 180 190 200
YATLPREKLS VFKLRTEARA HMEDEREATD CLVVEPAREL KGKDGEDLLT
210 220 230 240 250
GSRMTKVIAS TGRPRRRPYA AKIAQVARAK VGYLKNTPEN RLIYQRVMIE
260 270 280 290 300
IMDKDCVRYV DRDVILPLAI GCCFVYPDGV EESAALWGSD ESLGVKXGGL
310 320 330 340 350
VRLPGVVTQT NRDIPSGVLL PQEVLEVRAG PPNAKDRNIF MVAGCPSQAR
360 370 380 390 400
FLVHNHCLKN LKRGLVERVF CVERNGQLTR TPQPTKGAFG RLSPFRKAVC
410 420 430 440 450
EKVGVAHRLG YDGFLSYYSG AKLRTYTRAV ESLHITPVSE RDSHLTTFVK
460 470 480 490 500
AEKISTAKGD PAPRVIQPRN PRYNVELGRY LRHMESKLMK AVDGVFGETT
510 520 530 540 550
CIKGYTADEV GQIFRDKWDR FNKPVAIGLD ASRFDQHCSV EALQFEHGFY
560 570 580 590 600
RAMYPGNKLL SKLLDWQLHN KGKGYVPDGT ITYRKEGCRM SGDINTSLGN
610 620 630 640 650
YLLMCAMIYG YMRHLGINEY SLANCGDDCV LIVERRNLKQ IQGTLPEYFL
660 670 680 690 700
NLGYTMKVEP PVFQLEEVEF CQAHPVQFQG GWKMVRNVRT AMSKDVHCVN
710 720 730 740 750
NIRDLATRRA WSNAQHHGGV ALTSGIPVVE KFYSRFTLYE VPKKHQRIDT
760 770 780 790 800
VTNVHKWRGS GGDYVVTPEA RASFWAAFGL TGDEQLALED RLDRWEMDLF
810
GEEGVDAHEP SILDSAVA
Length:818
Mass (Da):92,120
Last modified:October 5, 2010 - v2
Checksum:i0CE624521246706A
GO

Sequence cautioni

The sequence AAA42902.1 differs from that shown.Amino acid integrated instead of terminator codon missing.Curated

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
M25270 Genomic RNA. Translation: AAA42902.1. Sequence problems.
PIRiJA0130. RRVGCN.
RefSeqiNP_040953.2. NC_001469.1.

Genome annotation databases

GeneIDi1493946.
KEGGivg:1493946.

Keywords - Coding sequence diversityi

RNA suppression of termination

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
M25270 Genomic RNA. Translation: AAA42902.1. Sequence problems.
PIRiJA0130. RRVGCN.
RefSeqiNP_040953.2. NC_001469.1.

3D structure databases

ProteinModelPortaliP15187.
ModBaseiSearch...
MobiDBiSearch...

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

GeneIDi1493946.
KEGGivg:1493946.

Family and domain databases

InterProiIPR007094. RNA-dir_pol_PSvirus.
IPR002166. RNA_pol_HCV.
IPR013707. Tombusvirus_p33.
[Graphical view]
PfamiPF00998. RdRP_3. 1 hit.
PF08500. Tombus_P33. 1 hit.
[Graphical view]
PROSITEiPS50507. RDRP_SSRNA_POS. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

  1. "Complete nucleotide sequence of the cucumber necrosis virus genome."
    Rochon D.M., Tremaine J.H.
    Virology 169:251-259(1988) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC RNA].

Entry informationi

Entry nameiRDRP_CNV
AccessioniPrimary (citable) accession number: P15187
Entry historyi
Integrated into UniProtKB/Swiss-Prot: April 1, 1990
Last sequence update: October 5, 2010
Last modified: April 1, 2015
This is version 75 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programViral Protein Annotation Program

Miscellaneousi

Miscellaneous

Readthrough of the terminator UAG occurs at position 297.

Keywords - Technical termi

Complete proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.