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P15181

- SYLM_NEUCR

UniProt

P15181 - SYLM_NEUCR

Protein

Leucine--tRNA ligase, mitochondrial

Gene

leu-5

Organism
Neurospora crassa (strain ATCC 24698 / 74-OR23-1A / CBS 708.71 / DSM 1257 / FGSC 987)
Status
Reviewed - Annotation score: 3 out of 5- Protein inferred from homologyi
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    • History
      Entry version 125 (01 Oct 2014)
      Sequence version 1 (01 Apr 1990)
      Previous versions | rss
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    Functioni

    Catalytic activityi

    ATP + L-leucine + tRNA(Leu) = AMP + diphosphate + L-leucyl-tRNA(Leu).

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Binding sitei700 – 7001ATPBy similarity

    GO - Molecular functioni

    1. aminoacyl-tRNA editing activity Source: InterPro
    2. ATP binding Source: UniProtKB-KW
    3. leucine-tRNA ligase activity Source: UniProtKB-EC
    4. mRNA binding Source: EnsemblFungi

    GO - Biological processi

    1. Group I intron splicing Source: EnsemblFungi
    2. leucyl-tRNA aminoacylation Source: InterPro
    3. mitochondrial translation Source: EnsemblFungi

    Keywords - Molecular functioni

    Aminoacyl-tRNA synthetase, Ligase

    Keywords - Biological processi

    Protein biosynthesis

    Keywords - Ligandi

    ATP-binding, Nucleotide-binding

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Leucine--tRNA ligase, mitochondrial (EC:6.1.1.4)
    Alternative name(s):
    Leucyl-tRNA synthetase
    Short name:
    LeuRS
    Gene namesi
    Name:leu-5
    ORF Names:99H12.170, NCU03814
    OrganismiNeurospora crassa (strain ATCC 24698 / 74-OR23-1A / CBS 708.71 / DSM 1257 / FGSC 987)
    Taxonomic identifieri367110 [NCBI]
    Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaPezizomycotinaSordariomycetesSordariomycetidaeSordarialesSordariaceaeNeurospora
    ProteomesiUP000001805: Chromosome 2, Linkage Group V

    Subcellular locationi

    GO - Cellular componenti

    1. mitochondrial matrix Source: UniProtKB-SubCell

    Keywords - Cellular componenti

    Mitochondrion

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Transit peptidei1 – 5252MitochondrionAdd
    BLAST
    Chaini53 – 994942Leucine--tRNA ligase, mitochondrialPRO_0000035808Add
    BLAST

    Interactioni

    Protein-protein interaction databases

    STRINGi5141.NCU03814.1.

    Structurei

    3D structure databases

    ProteinModelPortaliP15181.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Motif

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Motifi93 – 10311"HIGH" regionAdd
    BLAST
    Motifi697 – 7015"KMSKS" region

    Sequence similaritiesi

    Keywords - Domaini

    Transit peptide

    Phylogenomic databases

    eggNOGiCOG0495.
    HOGENOMiHOG000200747.
    KOiK01869.
    OMAiKGNPLES.
    OrthoDBiEOG7Q8CWM.

    Family and domain databases

    Gene3Di1.10.730.10. 1 hit.
    3.40.50.620. 3 hits.
    3.90.740.10. 1 hit.
    HAMAPiMF_00049_B. Leu_tRNA_synth_B.
    InterProiIPR001412. aa-tRNA-synth_I_CS.
    IPR002300. aa-tRNA-synth_Ia.
    IPR002302. Leu-tRNA-ligase.
    IPR025709. Leu_tRNA-synth_edit.
    IPR014729. Rossmann-like_a/b/a_fold.
    IPR009080. tRNAsynth_1a_anticodon-bd.
    IPR013155. V/L/I-tRNA-synth_anticodon-bd.
    IPR009008. Val/Leu/Ile-tRNA-synth_edit.
    [Graphical view]
    PANTHERiPTHR11946:SF7. PTHR11946:SF7. 1 hit.
    PfamiPF08264. Anticodon_1. 1 hit.
    PF00133. tRNA-synt_1. 3 hits.
    PF13603. tRNA-synt_1_2. 1 hit.
    [Graphical view]
    PRINTSiPR00985. TRNASYNTHLEU.
    SUPFAMiSSF47323. SSF47323. 1 hit.
    SSF50677. SSF50677. 1 hit.
    TIGRFAMsiTIGR00396. leuS_bact. 1 hit.
    PROSITEiPS00178. AA_TRNA_LIGASE_I. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    Sequence processingi: The displayed sequence is further processed into a mature form.

    P15181-1 [UniParc]FASTAAdd to Basket

    « Hide

    MPLICARPLG RLVPKLGASL RPVLSSHAAS PRRPVGVALE QHLGTESWKR    50
    FYADHKLDLL ALDQKWRQKW AESSREKGNK EDEKNKYVLP MFPYPSGHLH 100
    LGHLRVYTIA DVIARFQTLQ GHKVLLPMGW DAFGLPAENA AIERGINPAT 150
    WTKANIAKMK EQLGHMNGSW DWNCELATCD PDFYKHTQKI FLALHEKGLA 200
    YQAEAEVNYD PVDKTVLANE QVDANGCSWR SGARVEKRKL KQWFLKISEF 250
    RESLLKDLET LAKNEAWPER VLAMQKNWLG KSKGATVKFP VLAFGQGTPS 300
    AIEVFTSRPD TLFGVQYIAL AATHPSVQQL AKSDPELQAF LSTLPGLSPD 350
    SKVGYLLPHI RAVNPLAYHE ETPEDTKVSL PIYVAPYVLG DYGEGAVMGV 400
    PGHDLRDHAF WKEHHYDAPV RFVLAASEDE STTAMPNEPF TEHGVMNANS 450
    GIFKGKSSKE AGEMLVKLLE PAGLAKETEK WRLRDWLISR QRYWGTPIPI 500
    VHCGSCGTVP VPDEQLPVEL PEVDEHWAGK KTGNPLESQT DWINTSCPKC 550
    GGEAKRDTDT MDTFVDSSWY YMRFIDAHNK EAPFSPEKAK VLTPVDLYIG 600
    GVEHAILHLL YSRFIYKFLM TSSFAGKEAE SAEAAESASS EVYEPFKRLI 650
    TQGMVHGKTY TDPATGRFLK PDEVDLSDPH QPKVVATGAL ANVSYEKMSK 700
    SKHNGVDPTT FIAQYGADAT RAHILFQAPV SEILDWDESK ITGVTRWLSR 750
    VHDLVQKIAC SSTSETPSSA STVKAFFEQQ QQQPESVADP AKLDASITIW 800
    REVQRTISSV TASYNKVYTL NTVISDLMSL TNVIASPSNY DAADPLIRRE 850
    AVSALIRMMA PVAPAFAEEC WHVLFPESSS SSLFSGSGSG SGSGSGDEQA 900
    ARFPVPDGTE GLLKSRKQTC AVQLNGKTKF AVEIGTPPAG LLEKSAEEKL 950
    REFIVGEVLK TEEGRAKLEG RGVDVSKAKK VIVVRGGKLL NVVM 994
    Length:994
    Mass (Da):109,854
    Last modified:April 1, 1990 - v1
    Checksum:i2ADB26302A8465F9
    GO

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    M30472 Genomic DNA. Translation: AAA33599.1.
    AL451018 Genomic DNA. Translation: CAC18253.1.
    CM002240 Genomic DNA. Translation: EAA31967.1.
    PIRiA33474. SYNCLM.
    RefSeqiXP_961203.1. XM_956110.1.
    UniGeneiNcr.25061.

    Genome annotation databases

    EnsemblFungiiEFNCRT00000003503; EFNCRP00000003503; EFNCRG00000003499.
    GeneIDi3877331.
    KEGGincr:NCU03814.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    M30472 Genomic DNA. Translation: AAA33599.1 .
    AL451018 Genomic DNA. Translation: CAC18253.1 .
    CM002240 Genomic DNA. Translation: EAA31967.1 .
    PIRi A33474. SYNCLM.
    RefSeqi XP_961203.1. XM_956110.1.
    UniGenei Ncr.25061.

    3D structure databases

    ProteinModelPortali P15181.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    STRINGi 5141.NCU03814.1.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    EnsemblFungii EFNCRT00000003503 ; EFNCRP00000003503 ; EFNCRG00000003499 .
    GeneIDi 3877331.
    KEGGi ncr:NCU03814.

    Phylogenomic databases

    eggNOGi COG0495.
    HOGENOMi HOG000200747.
    KOi K01869.
    OMAi KGNPLES.
    OrthoDBi EOG7Q8CWM.

    Family and domain databases

    Gene3Di 1.10.730.10. 1 hit.
    3.40.50.620. 3 hits.
    3.90.740.10. 1 hit.
    HAMAPi MF_00049_B. Leu_tRNA_synth_B.
    InterProi IPR001412. aa-tRNA-synth_I_CS.
    IPR002300. aa-tRNA-synth_Ia.
    IPR002302. Leu-tRNA-ligase.
    IPR025709. Leu_tRNA-synth_edit.
    IPR014729. Rossmann-like_a/b/a_fold.
    IPR009080. tRNAsynth_1a_anticodon-bd.
    IPR013155. V/L/I-tRNA-synth_anticodon-bd.
    IPR009008. Val/Leu/Ile-tRNA-synth_edit.
    [Graphical view ]
    PANTHERi PTHR11946:SF7. PTHR11946:SF7. 1 hit.
    Pfami PF08264. Anticodon_1. 1 hit.
    PF00133. tRNA-synt_1. 3 hits.
    PF13603. tRNA-synt_1_2. 1 hit.
    [Graphical view ]
    PRINTSi PR00985. TRNASYNTHLEU.
    SUPFAMi SSF47323. SSF47323. 1 hit.
    SSF50677. SSF50677. 1 hit.
    TIGRFAMsi TIGR00396. leuS_bact. 1 hit.
    PROSITEi PS00178. AA_TRNA_LIGASE_I. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "Nuclear gene for mitochondrial leucyl-tRNA synthetase of Neurospora crassa: isolation, sequence, chromosomal mapping, and evidence that the leu-5 locus specifies structural information."
      Chow C.M., Metzenberg R.L., RajBhandary U.L.
      Mol. Cell. Biol. 9:4631-4644(1989) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
    2. "What's in the genome of a filamentous fungus? Analysis of the Neurospora genome sequence."
      Mannhaupt G., Montrone C., Haase D., Mewes H.-W., Aign V., Hoheisel J.D., Fartmann B., Nyakatura G., Kempken F., Maier J., Schulte U.
      Nucleic Acids Res. 31:1944-1954(2003) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: ATCC 24698 / 74-OR23-1A / CBS 708.71 / DSM 1257 / FGSC 987.
    3. "The genome sequence of the filamentous fungus Neurospora crassa."
      Galagan J.E., Calvo S.E., Borkovich K.A., Selker E.U., Read N.D., Jaffe D.B., FitzHugh W., Ma L.-J., Smirnov S., Purcell S., Rehman B., Elkins T., Engels R., Wang S., Nielsen C.B., Butler J., Endrizzi M., Qui D.
      , Ianakiev P., Bell-Pedersen D., Nelson M.A., Werner-Washburne M., Selitrennikoff C.P., Kinsey J.A., Braun E.L., Zelter A., Schulte U., Kothe G.O., Jedd G., Mewes H.-W., Staben C., Marcotte E., Greenberg D., Roy A., Foley K., Naylor J., Stange-Thomann N., Barrett R., Gnerre S., Kamal M., Kamvysselis M., Mauceli E.W., Bielke C., Rudd S., Frishman D., Krystofova S., Rasmussen C., Metzenberg R.L., Perkins D.D., Kroken S., Cogoni C., Macino G., Catcheside D.E.A., Li W., Pratt R.J., Osmani S.A., DeSouza C.P.C., Glass N.L., Orbach M.J., Berglund J.A., Voelker R., Yarden O., Plamann M., Seiler S., Dunlap J.C., Radford A., Aramayo R., Natvig D.O., Alex L.A., Mannhaupt G., Ebbole D.J., Freitag M., Paulsen I., Sachs M.S., Lander E.S., Nusbaum C., Birren B.W.
      Nature 422:859-868(2003) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: ATCC 24698 / 74-OR23-1A / CBS 708.71 / DSM 1257 / FGSC 987.

    Entry informationi

    Entry nameiSYLM_NEUCR
    AccessioniPrimary (citable) accession number: P15181
    Secondary accession number(s): Q7RVC8
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: April 1, 1990
    Last sequence update: April 1, 1990
    Last modified: October 1, 2014
    This is version 125 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programFungal Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. Aminoacyl-tRNA synthetases
      List of aminoacyl-tRNA synthetase entries
    2. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3