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P15181

- SYLM_NEUCR

UniProt

P15181 - SYLM_NEUCR

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Protein

Leucine--tRNA ligase, mitochondrial

Gene

leu-5

Organism
Neurospora crassa (strain ATCC 24698 / 74-OR23-1A / CBS 708.71 / DSM 1257 / FGSC 987)
Status
Reviewed - Annotation score: 3 out of 5- Protein inferred from homologyi

Functioni

Catalytic activityi

ATP + L-leucine + tRNA(Leu) = AMP + diphosphate + L-leucyl-tRNA(Leu).

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei700 – 7001ATPBy similarity

GO - Molecular functioni

  1. aminoacyl-tRNA editing activity Source: InterPro
  2. ATP binding Source: UniProtKB-KW
  3. leucine-tRNA ligase activity Source: UniProtKB-EC
  4. mRNA binding Source: EnsemblFungi

GO - Biological processi

  1. Group I intron splicing Source: EnsemblFungi
  2. leucyl-tRNA aminoacylation Source: InterPro
  3. mitochondrial translation Source: EnsemblFungi
Complete GO annotation...

Keywords - Molecular functioni

Aminoacyl-tRNA synthetase, Ligase

Keywords - Biological processi

Protein biosynthesis

Keywords - Ligandi

ATP-binding, Nucleotide-binding

Names & Taxonomyi

Protein namesi
Recommended name:
Leucine--tRNA ligase, mitochondrial (EC:6.1.1.4)
Alternative name(s):
Leucyl-tRNA synthetase
Short name:
LeuRS
Gene namesi
Name:leu-5
ORF Names:99H12.170, NCU03814
OrganismiNeurospora crassa (strain ATCC 24698 / 74-OR23-1A / CBS 708.71 / DSM 1257 / FGSC 987)
Taxonomic identifieri367110 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaPezizomycotinaSordariomycetesSordariomycetidaeSordarialesSordariaceaeNeurospora
ProteomesiUP000001805: Chromosome 2, Linkage Group V

Subcellular locationi

GO - Cellular componenti

  1. mitochondrion Source: UniProtKB-KW
Complete GO annotation...

Keywords - Cellular componenti

Mitochondrion

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Transit peptidei1 – 5252MitochondrionAdd
BLAST
Chaini53 – 994942Leucine--tRNA ligase, mitochondrialPRO_0000035808Add
BLAST

Interactioni

Protein-protein interaction databases

STRINGi5141.NCU03814.1.

Structurei

3D structure databases

ProteinModelPortaliP15181.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Motif

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Motifi93 – 10311"HIGH" regionAdd
BLAST
Motifi697 – 7015"KMSKS" region

Sequence similaritiesi

Keywords - Domaini

Transit peptide

Phylogenomic databases

eggNOGiCOG0495.
HOGENOMiHOG000200747.
InParanoidiP15181.
KOiK01869.
OMAiKGNPLES.
OrthoDBiEOG7Q8CWM.

Family and domain databases

Gene3Di1.10.730.10. 1 hit.
3.40.50.620. 3 hits.
3.90.740.10. 1 hit.
HAMAPiMF_00049_B. Leu_tRNA_synth_B.
InterProiIPR001412. aa-tRNA-synth_I_CS.
IPR002300. aa-tRNA-synth_Ia.
IPR002302. Leu-tRNA-ligase.
IPR025709. Leu_tRNA-synth_edit.
IPR014729. Rossmann-like_a/b/a_fold.
IPR009080. tRNAsynth_1a_anticodon-bd.
IPR013155. V/L/I-tRNA-synth_anticodon-bd.
IPR009008. Val/Leu/Ile-tRNA-synth_edit.
[Graphical view]
PANTHERiPTHR11946:SF7. PTHR11946:SF7. 1 hit.
PfamiPF08264. Anticodon_1. 1 hit.
PF00133. tRNA-synt_1. 3 hits.
PF13603. tRNA-synt_1_2. 1 hit.
[Graphical view]
PRINTSiPR00985. TRNASYNTHLEU.
SUPFAMiSSF47323. SSF47323. 1 hit.
SSF50677. SSF50677. 1 hit.
TIGRFAMsiTIGR00396. leuS_bact. 1 hit.
PROSITEiPS00178. AA_TRNA_LIGASE_I. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

P15181-1 [UniParc]FASTAAdd to Basket

« Hide

        10         20         30         40         50
MPLICARPLG RLVPKLGASL RPVLSSHAAS PRRPVGVALE QHLGTESWKR
60 70 80 90 100
FYADHKLDLL ALDQKWRQKW AESSREKGNK EDEKNKYVLP MFPYPSGHLH
110 120 130 140 150
LGHLRVYTIA DVIARFQTLQ GHKVLLPMGW DAFGLPAENA AIERGINPAT
160 170 180 190 200
WTKANIAKMK EQLGHMNGSW DWNCELATCD PDFYKHTQKI FLALHEKGLA
210 220 230 240 250
YQAEAEVNYD PVDKTVLANE QVDANGCSWR SGARVEKRKL KQWFLKISEF
260 270 280 290 300
RESLLKDLET LAKNEAWPER VLAMQKNWLG KSKGATVKFP VLAFGQGTPS
310 320 330 340 350
AIEVFTSRPD TLFGVQYIAL AATHPSVQQL AKSDPELQAF LSTLPGLSPD
360 370 380 390 400
SKVGYLLPHI RAVNPLAYHE ETPEDTKVSL PIYVAPYVLG DYGEGAVMGV
410 420 430 440 450
PGHDLRDHAF WKEHHYDAPV RFVLAASEDE STTAMPNEPF TEHGVMNANS
460 470 480 490 500
GIFKGKSSKE AGEMLVKLLE PAGLAKETEK WRLRDWLISR QRYWGTPIPI
510 520 530 540 550
VHCGSCGTVP VPDEQLPVEL PEVDEHWAGK KTGNPLESQT DWINTSCPKC
560 570 580 590 600
GGEAKRDTDT MDTFVDSSWY YMRFIDAHNK EAPFSPEKAK VLTPVDLYIG
610 620 630 640 650
GVEHAILHLL YSRFIYKFLM TSSFAGKEAE SAEAAESASS EVYEPFKRLI
660 670 680 690 700
TQGMVHGKTY TDPATGRFLK PDEVDLSDPH QPKVVATGAL ANVSYEKMSK
710 720 730 740 750
SKHNGVDPTT FIAQYGADAT RAHILFQAPV SEILDWDESK ITGVTRWLSR
760 770 780 790 800
VHDLVQKIAC SSTSETPSSA STVKAFFEQQ QQQPESVADP AKLDASITIW
810 820 830 840 850
REVQRTISSV TASYNKVYTL NTVISDLMSL TNVIASPSNY DAADPLIRRE
860 870 880 890 900
AVSALIRMMA PVAPAFAEEC WHVLFPESSS SSLFSGSGSG SGSGSGDEQA
910 920 930 940 950
ARFPVPDGTE GLLKSRKQTC AVQLNGKTKF AVEIGTPPAG LLEKSAEEKL
960 970 980 990
REFIVGEVLK TEEGRAKLEG RGVDVSKAKK VIVVRGGKLL NVVM
Length:994
Mass (Da):109,854
Last modified:April 1, 1990 - v1
Checksum:i2ADB26302A8465F9
GO

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
M30472 Genomic DNA. Translation: AAA33599.1.
AL451018 Genomic DNA. Translation: CAC18253.1.
CM002240 Genomic DNA. Translation: EAA31967.1.
PIRiA33474. SYNCLM.
RefSeqiXP_961203.1. XM_956110.1.
UniGeneiNcr.25061.

Genome annotation databases

EnsemblFungiiEFNCRT00000003503; EFNCRP00000003503; EFNCRG00000003499.
GeneIDi3877331.
KEGGincr:NCU03814.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
M30472 Genomic DNA. Translation: AAA33599.1 .
AL451018 Genomic DNA. Translation: CAC18253.1 .
CM002240 Genomic DNA. Translation: EAA31967.1 .
PIRi A33474. SYNCLM.
RefSeqi XP_961203.1. XM_956110.1.
UniGenei Ncr.25061.

3D structure databases

ProteinModelPortali P15181.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

STRINGi 5141.NCU03814.1.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

EnsemblFungii EFNCRT00000003503 ; EFNCRP00000003503 ; EFNCRG00000003499 .
GeneIDi 3877331.
KEGGi ncr:NCU03814.

Phylogenomic databases

eggNOGi COG0495.
HOGENOMi HOG000200747.
InParanoidi P15181.
KOi K01869.
OMAi KGNPLES.
OrthoDBi EOG7Q8CWM.

Family and domain databases

Gene3Di 1.10.730.10. 1 hit.
3.40.50.620. 3 hits.
3.90.740.10. 1 hit.
HAMAPi MF_00049_B. Leu_tRNA_synth_B.
InterProi IPR001412. aa-tRNA-synth_I_CS.
IPR002300. aa-tRNA-synth_Ia.
IPR002302. Leu-tRNA-ligase.
IPR025709. Leu_tRNA-synth_edit.
IPR014729. Rossmann-like_a/b/a_fold.
IPR009080. tRNAsynth_1a_anticodon-bd.
IPR013155. V/L/I-tRNA-synth_anticodon-bd.
IPR009008. Val/Leu/Ile-tRNA-synth_edit.
[Graphical view ]
PANTHERi PTHR11946:SF7. PTHR11946:SF7. 1 hit.
Pfami PF08264. Anticodon_1. 1 hit.
PF00133. tRNA-synt_1. 3 hits.
PF13603. tRNA-synt_1_2. 1 hit.
[Graphical view ]
PRINTSi PR00985. TRNASYNTHLEU.
SUPFAMi SSF47323. SSF47323. 1 hit.
SSF50677. SSF50677. 1 hit.
TIGRFAMsi TIGR00396. leuS_bact. 1 hit.
PROSITEi PS00178. AA_TRNA_LIGASE_I. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

« Hide 'large scale' publications
  1. "Nuclear gene for mitochondrial leucyl-tRNA synthetase of Neurospora crassa: isolation, sequence, chromosomal mapping, and evidence that the leu-5 locus specifies structural information."
    Chow C.M., Metzenberg R.L., RajBhandary U.L.
    Mol. Cell. Biol. 9:4631-4644(1989) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
  2. "What's in the genome of a filamentous fungus? Analysis of the Neurospora genome sequence."
    Mannhaupt G., Montrone C., Haase D., Mewes H.-W., Aign V., Hoheisel J.D., Fartmann B., Nyakatura G., Kempken F., Maier J., Schulte U.
    Nucleic Acids Res. 31:1944-1954(2003) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: ATCC 24698 / 74-OR23-1A / CBS 708.71 / DSM 1257 / FGSC 987.
  3. "The genome sequence of the filamentous fungus Neurospora crassa."
    Galagan J.E., Calvo S.E., Borkovich K.A., Selker E.U., Read N.D., Jaffe D.B., FitzHugh W., Ma L.-J., Smirnov S., Purcell S., Rehman B., Elkins T., Engels R., Wang S., Nielsen C.B., Butler J., Endrizzi M., Qui D.
    , Ianakiev P., Bell-Pedersen D., Nelson M.A., Werner-Washburne M., Selitrennikoff C.P., Kinsey J.A., Braun E.L., Zelter A., Schulte U., Kothe G.O., Jedd G., Mewes H.-W., Staben C., Marcotte E., Greenberg D., Roy A., Foley K., Naylor J., Stange-Thomann N., Barrett R., Gnerre S., Kamal M., Kamvysselis M., Mauceli E.W., Bielke C., Rudd S., Frishman D., Krystofova S., Rasmussen C., Metzenberg R.L., Perkins D.D., Kroken S., Cogoni C., Macino G., Catcheside D.E.A., Li W., Pratt R.J., Osmani S.A., DeSouza C.P.C., Glass N.L., Orbach M.J., Berglund J.A., Voelker R., Yarden O., Plamann M., Seiler S., Dunlap J.C., Radford A., Aramayo R., Natvig D.O., Alex L.A., Mannhaupt G., Ebbole D.J., Freitag M., Paulsen I., Sachs M.S., Lander E.S., Nusbaum C., Birren B.W.
    Nature 422:859-868(2003) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: ATCC 24698 / 74-OR23-1A / CBS 708.71 / DSM 1257 / FGSC 987.

Entry informationi

Entry nameiSYLM_NEUCR
AccessioniPrimary (citable) accession number: P15181
Secondary accession number(s): Q7RVC8
Entry historyi
Integrated into UniProtKB/Swiss-Prot: April 1, 1990
Last sequence update: April 1, 1990
Last modified: October 29, 2014
This is version 126 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Aminoacyl-tRNA synthetases
    List of aminoacyl-tRNA synthetase entries
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3