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P15181 (SYLM_NEUCR) Reviewed, UniProtKB/Swiss-Prot

Last modified April 16, 2014. Version 123. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Leucine--tRNA ligase, mitochondrial

EC=6.1.1.4
Alternative name(s):
Leucyl-tRNA synthetase
Short name=LeuRS
Gene names
Name:leu-5
ORF Names:99H12.170, NCU03814
OrganismNeurospora crassa (strain ATCC 24698 / 74-OR23-1A / CBS 708.71 / DSM 1257 / FGSC 987) [Reference proteome]
Taxonomic identifier367110 [NCBI]
Taxonomic lineageEukaryotaFungiDikaryaAscomycotaPezizomycotinaSordariomycetesSordariomycetidaeSordarialesSordariaceaeNeurospora

Protein attributes

Sequence length994 AA.
Sequence statusComplete.
Sequence processingThe displayed sequence is further processed into a mature form.
Protein existenceInferred from homology

General annotation (Comments)

Catalytic activity

ATP + L-leucine + tRNA(Leu) = AMP + diphosphate + L-leucyl-tRNA(Leu). HAMAP-Rule MF_00049_B

Subcellular location

Mitochondrion matrix HAMAP-Rule MF_00049_B.

Sequence similarities

Belongs to the class-I aminoacyl-tRNA synthetase family.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Transit peptide1 – 5252Mitochondrion HAMAP-Rule MF_00049_B
Chain53 – 994942Leucine--tRNA ligase, mitochondrial HAMAP-Rule MF_00049_B
PRO_0000035808

Regions

Motif93 – 10311"HIGH" region HAMAP-Rule MF_00049_B
Motif697 – 7015"KMSKS" region HAMAP-Rule MF_00049_B

Sites

Binding site7001ATP By similarity

Sequences

Sequence LengthMass (Da)Tools
P15181 [UniParc].

Last modified April 1, 1990. Version 1.
Checksum: 2ADB26302A8465F9

FASTA994109,854
        10         20         30         40         50         60 
MPLICARPLG RLVPKLGASL RPVLSSHAAS PRRPVGVALE QHLGTESWKR FYADHKLDLL 

        70         80         90        100        110        120 
ALDQKWRQKW AESSREKGNK EDEKNKYVLP MFPYPSGHLH LGHLRVYTIA DVIARFQTLQ 

       130        140        150        160        170        180 
GHKVLLPMGW DAFGLPAENA AIERGINPAT WTKANIAKMK EQLGHMNGSW DWNCELATCD 

       190        200        210        220        230        240 
PDFYKHTQKI FLALHEKGLA YQAEAEVNYD PVDKTVLANE QVDANGCSWR SGARVEKRKL 

       250        260        270        280        290        300 
KQWFLKISEF RESLLKDLET LAKNEAWPER VLAMQKNWLG KSKGATVKFP VLAFGQGTPS 

       310        320        330        340        350        360 
AIEVFTSRPD TLFGVQYIAL AATHPSVQQL AKSDPELQAF LSTLPGLSPD SKVGYLLPHI 

       370        380        390        400        410        420 
RAVNPLAYHE ETPEDTKVSL PIYVAPYVLG DYGEGAVMGV PGHDLRDHAF WKEHHYDAPV 

       430        440        450        460        470        480 
RFVLAASEDE STTAMPNEPF TEHGVMNANS GIFKGKSSKE AGEMLVKLLE PAGLAKETEK 

       490        500        510        520        530        540 
WRLRDWLISR QRYWGTPIPI VHCGSCGTVP VPDEQLPVEL PEVDEHWAGK KTGNPLESQT 

       550        560        570        580        590        600 
DWINTSCPKC GGEAKRDTDT MDTFVDSSWY YMRFIDAHNK EAPFSPEKAK VLTPVDLYIG 

       610        620        630        640        650        660 
GVEHAILHLL YSRFIYKFLM TSSFAGKEAE SAEAAESASS EVYEPFKRLI TQGMVHGKTY 

       670        680        690        700        710        720 
TDPATGRFLK PDEVDLSDPH QPKVVATGAL ANVSYEKMSK SKHNGVDPTT FIAQYGADAT 

       730        740        750        760        770        780 
RAHILFQAPV SEILDWDESK ITGVTRWLSR VHDLVQKIAC SSTSETPSSA STVKAFFEQQ 

       790        800        810        820        830        840 
QQQPESVADP AKLDASITIW REVQRTISSV TASYNKVYTL NTVISDLMSL TNVIASPSNY 

       850        860        870        880        890        900 
DAADPLIRRE AVSALIRMMA PVAPAFAEEC WHVLFPESSS SSLFSGSGSG SGSGSGDEQA 

       910        920        930        940        950        960 
ARFPVPDGTE GLLKSRKQTC AVQLNGKTKF AVEIGTPPAG LLEKSAEEKL REFIVGEVLK 

       970        980        990 
TEEGRAKLEG RGVDVSKAKK VIVVRGGKLL NVVM 

« Hide

References

« Hide 'large scale' references
[1]"Nuclear gene for mitochondrial leucyl-tRNA synthetase of Neurospora crassa: isolation, sequence, chromosomal mapping, and evidence that the leu-5 locus specifies structural information."
Chow C.M., Metzenberg R.L., RajBhandary U.L.
Mol. Cell. Biol. 9:4631-4644(1989) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
[2]"What's in the genome of a filamentous fungus? Analysis of the Neurospora genome sequence."
Mannhaupt G., Montrone C., Haase D., Mewes H.-W., Aign V., Hoheisel J.D., Fartmann B., Nyakatura G., Kempken F., Maier J., Schulte U.
Nucleic Acids Res. 31:1944-1954(2003) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: ATCC 24698 / 74-OR23-1A / CBS 708.71 / DSM 1257 / FGSC 987.
[3]"The genome sequence of the filamentous fungus Neurospora crassa."
Galagan J.E., Calvo S.E., Borkovich K.A., Selker E.U., Read N.D., Jaffe D.B., FitzHugh W., Ma L.-J., Smirnov S., Purcell S., Rehman B., Elkins T., Engels R., Wang S., Nielsen C.B., Butler J., Endrizzi M., Qui D. expand/collapse author list , Ianakiev P., Bell-Pedersen D., Nelson M.A., Werner-Washburne M., Selitrennikoff C.P., Kinsey J.A., Braun E.L., Zelter A., Schulte U., Kothe G.O., Jedd G., Mewes H.-W., Staben C., Marcotte E., Greenberg D., Roy A., Foley K., Naylor J., Stange-Thomann N., Barrett R., Gnerre S., Kamal M., Kamvysselis M., Mauceli E.W., Bielke C., Rudd S., Frishman D., Krystofova S., Rasmussen C., Metzenberg R.L., Perkins D.D., Kroken S., Cogoni C., Macino G., Catcheside D.E.A., Li W., Pratt R.J., Osmani S.A., DeSouza C.P.C., Glass N.L., Orbach M.J., Berglund J.A., Voelker R., Yarden O., Plamann M., Seiler S., Dunlap J.C., Radford A., Aramayo R., Natvig D.O., Alex L.A., Mannhaupt G., Ebbole D.J., Freitag M., Paulsen I., Sachs M.S., Lander E.S., Nusbaum C., Birren B.W.
Nature 422:859-868(2003) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: ATCC 24698 / 74-OR23-1A / CBS 708.71 / DSM 1257 / FGSC 987.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
M30472 Genomic DNA. Translation: AAA33599.1.
AL451018 Genomic DNA. Translation: CAC18253.1.
CM002240 Genomic DNA. Translation: EAA31967.1.
PIRSYNCLM. A33474.
RefSeqXP_961203.1. XM_956110.1.
UniGeneNcr.25061.

3D structure databases

ProteinModelPortalP15181.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

STRING5141.NCU03814.1.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblFungiEFNCRT00000003503; EFNCRP00000003503; EFNCRG00000003499.
GeneID3877331.
KEGGncr:NCU03814.

Phylogenomic databases

eggNOGCOG0495.
HOGENOMHOG000200747.
KOK01869.
OMAREKNDWE.
OrthoDBEOG7Q8CWM.

Family and domain databases

Gene3D1.10.730.10. 1 hit.
3.40.50.620. 3 hits.
3.90.740.10. 1 hit.
HAMAPMF_00049_B. Leu_tRNA_synth_B.
InterProIPR001412. aa-tRNA-synth_I_CS.
IPR002300. aa-tRNA-synth_Ia.
IPR002302. Leu-tRNA-ligase_bac/mito.
IPR025709. Leu_tRNA-synth_edit.
IPR014729. Rossmann-like_a/b/a_fold.
IPR009080. tRNAsynth_1a_anticodon-bd.
IPR013155. V/L/I-tRNA-synth_anticodon-bd.
IPR009008. Val/Leu/Ile-tRNA-synth_edit.
[Graphical view]
PANTHERPTHR11946:SF7. PTHR11946:SF7. 1 hit.
PfamPF08264. Anticodon_1. 1 hit.
PF00133. tRNA-synt_1. 3 hits.
PF13603. tRNA-synt_1_2. 1 hit.
[Graphical view]
PRINTSPR00985. TRNASYNTHLEU.
SUPFAMSSF47323. SSF47323. 1 hit.
SSF50677. SSF50677. 1 hit.
TIGRFAMsTIGR00396. leuS_bact. 1 hit.
PROSITEPS00178. AA_TRNA_LIGASE_I. 1 hit.
[Graphical view]
ProtoNetSearch...

Entry information

Entry nameSYLM_NEUCR
AccessionPrimary (citable) accession number: P15181
Secondary accession number(s): Q7RVC8
Entry history
Integrated into UniProtKB/Swiss-Prot: April 1, 1990
Last sequence update: April 1, 1990
Last modified: April 16, 2014
This is version 123 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

Aminoacyl-tRNA synthetases

List of aminoacyl-tRNA synthetase entries