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Protein

Lysine--tRNA ligase, cytoplasmic

Gene

KRS1

Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Catalytic activityi

ATP + L-lysine + tRNA(Lys) = AMP + diphosphate + L-lysyl-tRNA(Lys).

GO - Molecular functioni

  • ATP binding Source: UniProtKB-KW
  • lysine-tRNA ligase activity Source: SGD
  • nucleic acid binding Source: InterPro

GO - Biological processi

  • lysyl-tRNA aminoacylation Source: SGD

Keywordsi

Molecular functionAminoacyl-tRNA synthetase, Ligase
Biological processProtein biosynthesis
LigandATP-binding, Nucleotide-binding

Enzyme and pathway databases

BioCyciYEAST:G3O-29651-MONOMER

Names & Taxonomyi

Protein namesi
Recommended name:
Lysine--tRNA ligase, cytoplasmic (EC:6.1.1.6)
Alternative name(s):
Lysyl-tRNA synthetase
Short name:
LysRS
Gene namesi
Name:KRS1
Synonyms:GCD5
Ordered Locus Names:YDR037W
ORF Names:YD9673.09
OrganismiSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Taxonomic identifieri559292 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces
Proteomesi
  • UP000002311 Componenti: Chromosome IV

Organism-specific databases

EuPathDBiFungiDB:YDR037W
SGDiS000002444 KRS1

Subcellular locationi

Keywords - Cellular componenti

Cytoplasm

Pathology & Biotechi

Mutagenesis

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Mutagenesisi488R → L in GCD5-1; defects in lysine-binding. 1 Publication1

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Initiator methionineiRemoved1 Publication
ChainiPRO_00001527712 – 591Lysine--tRNA ligase, cytoplasmicAdd BLAST590

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei2N-acetylserine1 Publication1
Modified residuei30PhosphoserineCombined sources1
Modified residuei62PhosphothreonineCombined sources1
Cross-linki308Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in ubiquitin)Combined sources
Cross-linki577Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in ubiquitin)Combined sources

Keywords - PTMi

Acetylation, Isopeptide bond, Phosphoprotein, Ubl conjugation

Proteomic databases

MaxQBiP15180
PaxDbiP15180
PRIDEiP15180

PTM databases

CarbonylDBiP15180
iPTMnetiP15180

Interactioni

Subunit structurei

Homodimer.

Protein-protein interaction databases

BioGridi32092, 157 interactors
DIPiDIP-6359N
IntActiP15180, 10 interactors
MINTiP15180
STRINGi4932.YDR037W

Structurei

3D structure databases

ProteinModelPortaliP15180
SMRiP15180
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Compositional bias

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Compositional biasi33 – 61Lys-rich (basic)Add BLAST29

Sequence similaritiesi

Phylogenomic databases

GeneTreeiENSGT00550000074841
HOGENOMiHOG000236577
InParanoidiP15180
KOiK04567
OMAiMMNIIPG
OrthoDBiEOG092C0ZU5

Family and domain databases

CDDicd00775 LysRS_core, 1 hit
HAMAPiMF_00252 Lys_tRNA_synth_class2, 1 hit
InterProiView protein in InterPro
IPR004364 aa-tRNA-synt_II
IPR006195 aa-tRNA-synth_II
IPR002313 Lys-tRNA-ligase_II
IPR034762 Lys-tRNA-ligase_II_bac/euk
IPR018149 Lys-tRNA-synth_II_C
IPR012340 NA-bd_OB-fold
IPR004365 NA-bd_OB_tRNA
PfamiView protein in Pfam
PF00152 tRNA-synt_2, 1 hit
PF01336 tRNA_anti-codon, 1 hit
PIRSFiPIRSF039101 LysRS2, 1 hit
PRINTSiPR00982 TRNASYNTHLYS
SUPFAMiSSF50249 SSF50249, 1 hit
TIGRFAMsiTIGR00499 lysS_bact, 1 hit
PROSITEiView protein in PROSITE
PS50862 AA_TRNA_LIGASE_II, 1 hit

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

P15180-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MSQQDNVKAA AEGVANLHLD EATGEMVSKS ELKKRIKQRQ VEAKKAAKKA
60 70 80 90 100
AAQPKPASKK KTDLFADLDP SQYFETRSRQ IQELRKTHEP NPYPHKFHVS
110 120 130 140 150
ISNPEFLAKY AHLKKGETLP EEKVSIAGRI HAKRESGSKL KFYVLHGDGV
160 170 180 190 200
EVQLMSQLQD YCDPDSYEKD HDLLKRGDIV GVEGYVGRTQ PKKGGEGEVS
210 220 230 240 250
VFVSRVQLLT PCLHMLPADH FGFKDQETRY RKRYLDLIMN KDARNRFITR
260 270 280 290 300
SEIIRYIRRF LDQRKFIEVE TPMMNVIAGG ATAKPFITHH NDLDMDMYMR
310 320 330 340 350
IAPELFLKQL VVGGLDRVYE IGRQFRNEGI DMTHNPEFTT CEFYQAYADV
360 370 380 390 400
YDLMDMTELM FSEMVKEITG SYIIKYHPDP ADPAKELELN FSRPWKRINM
410 420 430 440 450
IEELEKVFNV KFPSGDQLHT AETGEFLKKI LVDNKLECPP PLTNARMLDK
460 470 480 490 500
LVGELEDTCI NPTFIFGHPQ MMSPLAKYSR DQPGLCERFE VFVATKEICN
510 520 530 540 550
AYTELNDPFD QRARFEEQAR QKDQGDDEAQ LVDETFCNAL EYGLPPTGGW
560 570 580 590
GCGIDRLAMF LTDSNTIREV LLFPTLKPDV LREEVKKEEE N
Length:591
Mass (Da):67,959
Last modified:January 23, 2007 - v2
Checksum:i3CFBC6EC396B1CCF
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti22A → V in CAA39699 (PubMed:2046655).Curated1
Sequence conflicti51A → T in CAA39699 (PubMed:2046655).Curated1
Sequence conflicti111A → V in CAA39699 (PubMed:2046655).Curated1
Sequence conflicti162C → H in CAA39699 (PubMed:2046655).Curated1
Sequence conflicti165D → E in CAA39699 (PubMed:2046655).Curated1
Sequence conflicti259R → K in CAA39699 (PubMed:2046655).Curated1
Sequence conflicti430 – 432ILV → VLA in CAA39699 (PubMed:2046655).Curated3
Sequence conflicti591N → H in CAA39699 (PubMed:2046655).Curated1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
J04186 Genomic DNA Translation: AAA66916.1
X56259 Genomic DNA Translation: CAA39699.1
Z68196 Genomic DNA Translation: CAA92376.1
Z74333 Genomic DNA Translation: CAA98863.1
BK006938 Genomic DNA Translation: DAA11885.1
PIRiA92695 SYBYKT
RefSeqiNP_010322.1, NM_001180345.1

Genome annotation databases

EnsemblFungiiYDR037W; YDR037W; YDR037W
GeneIDi851607
KEGGisce:YDR037W

Similar proteinsi

Entry informationi

Entry nameiSYKC_YEAST
AccessioniPrimary (citable) accession number: P15180
Secondary accession number(s): D6VS25
Entry historyiIntegrated into UniProtKB/Swiss-Prot: April 1, 1990
Last sequence update: January 23, 2007
Last modified: March 28, 2018
This is version 182 of the entry and version 2 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Direct protein sequencing, Reference proteome
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Main funding by: National Institutes of Health