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Protein

Lysine--tRNA ligase, cytoplasmic

Gene

KRS1

Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Catalytic activityi

ATP + L-lysine + tRNA(Lys) = AMP + diphosphate + L-lysyl-tRNA(Lys).

GO - Molecular functioni

  • ATP binding Source: UniProtKB-KW
  • lysine-tRNA ligase activity Source: SGD
  • nucleic acid binding Source: InterPro

GO - Biological processi

  • lysyl-tRNA aminoacylation Source: SGD
Complete GO annotation...

Keywords - Molecular functioni

Aminoacyl-tRNA synthetase, Ligase

Keywords - Biological processi

Protein biosynthesis

Keywords - Ligandi

ATP-binding, Nucleotide-binding

Enzyme and pathway databases

BioCyciYEAST:G3O-29651-MONOMER.

Names & Taxonomyi

Protein namesi
Recommended name:
Lysine--tRNA ligase, cytoplasmic (EC:6.1.1.6)
Alternative name(s):
Lysyl-tRNA synthetase
Short name:
LysRS
Gene namesi
Name:KRS1
Synonyms:GCD5
Ordered Locus Names:YDR037W
ORF Names:YD9673.09
OrganismiSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Taxonomic identifieri559292 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces
Proteomesi
  • UP000002311 Componenti: Chromosome IV

Organism-specific databases

EuPathDBiFungiDB:YDR037W.
SGDiS000002444. KRS1.

Subcellular locationi

GO - Cellular componenti

  • cytoplasm Source: SGD
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

Pathology & Biotechi

Mutagenesis

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Mutagenesisi488 – 4881R → L in GCD5-1; defects in lysine-binding. 1 Publication

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Initiator methionineiRemoved1 Publication
Chaini2 – 591590Lysine--tRNA ligase, cytoplasmicPRO_0000152771Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei2 – 21N-acetylserine1 Publication
Modified residuei30 – 301PhosphoserineCombined sources
Modified residuei62 – 621PhosphothreonineCombined sources
Cross-linki308 – 308Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in ubiquitin)Combined sources
Cross-linki577 – 577Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in ubiquitin)Combined sources

Keywords - PTMi

Acetylation, Isopeptide bond, Phosphoprotein, Ubl conjugation

Proteomic databases

MaxQBiP15180.

PTM databases

iPTMnetiP15180.

Interactioni

Subunit structurei

Homodimer.

Protein-protein interaction databases

BioGridi32092. 91 interactions.
DIPiDIP-6359N.
IntActiP15180. 7 interactions.
MINTiMINT-644811.

Structurei

3D structure databases

ProteinModelPortaliP15180.
SMRiP15180. Positions 69-579.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Compositional bias

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Compositional biasi33 – 6129Lys-rich (basic)Add
BLAST

Sequence similaritiesi

Phylogenomic databases

GeneTreeiENSGT00550000074841.
HOGENOMiHOG000236577.
InParanoidiP15180.
KOiK04567.
OMAiHHNTLDI.
OrthoDBiEOG092C0ZU5.

Family and domain databases

CDDicd00775. LysRS_core. 1 hit.
Gene3Di2.40.50.140. 1 hit.
HAMAPiMF_00252. Lys_tRNA_synth_class2. 1 hit.
InterProiIPR004364. aa-tRNA-synt_II.
IPR018150. aa-tRNA-synt_II-like.
IPR006195. aa-tRNA-synth_II.
IPR002313. Lys-tRNA-ligase_II.
IPR018149. Lys-tRNA-synth_II_C.
IPR012340. NA-bd_OB-fold.
IPR004365. NA-bd_OB_tRNA.
[Graphical view]
PANTHERiPTHR22594. PTHR22594. 1 hit.
PfamiPF00152. tRNA-synt_2. 1 hit.
PF01336. tRNA_anti-codon. 1 hit.
[Graphical view]
PIRSFiPIRSF039101. LysRS2. 1 hit.
PRINTSiPR00982. TRNASYNTHLYS.
SUPFAMiSSF50249. SSF50249. 1 hit.
TIGRFAMsiTIGR00499. lysS_bact. 1 hit.
PROSITEiPS50862. AA_TRNA_LIGASE_II. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

P15180-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MSQQDNVKAA AEGVANLHLD EATGEMVSKS ELKKRIKQRQ VEAKKAAKKA
60 70 80 90 100
AAQPKPASKK KTDLFADLDP SQYFETRSRQ IQELRKTHEP NPYPHKFHVS
110 120 130 140 150
ISNPEFLAKY AHLKKGETLP EEKVSIAGRI HAKRESGSKL KFYVLHGDGV
160 170 180 190 200
EVQLMSQLQD YCDPDSYEKD HDLLKRGDIV GVEGYVGRTQ PKKGGEGEVS
210 220 230 240 250
VFVSRVQLLT PCLHMLPADH FGFKDQETRY RKRYLDLIMN KDARNRFITR
260 270 280 290 300
SEIIRYIRRF LDQRKFIEVE TPMMNVIAGG ATAKPFITHH NDLDMDMYMR
310 320 330 340 350
IAPELFLKQL VVGGLDRVYE IGRQFRNEGI DMTHNPEFTT CEFYQAYADV
360 370 380 390 400
YDLMDMTELM FSEMVKEITG SYIIKYHPDP ADPAKELELN FSRPWKRINM
410 420 430 440 450
IEELEKVFNV KFPSGDQLHT AETGEFLKKI LVDNKLECPP PLTNARMLDK
460 470 480 490 500
LVGELEDTCI NPTFIFGHPQ MMSPLAKYSR DQPGLCERFE VFVATKEICN
510 520 530 540 550
AYTELNDPFD QRARFEEQAR QKDQGDDEAQ LVDETFCNAL EYGLPPTGGW
560 570 580 590
GCGIDRLAMF LTDSNTIREV LLFPTLKPDV LREEVKKEEE N
Length:591
Mass (Da):67,959
Last modified:January 23, 2007 - v2
Checksum:i3CFBC6EC396B1CCF
GO

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti22 – 221A → V in CAA39699 (PubMed:2046655).Curated
Sequence conflicti51 – 511A → T in CAA39699 (PubMed:2046655).Curated
Sequence conflicti111 – 1111A → V in CAA39699 (PubMed:2046655).Curated
Sequence conflicti162 – 1621C → H in CAA39699 (PubMed:2046655).Curated
Sequence conflicti165 – 1651D → E in CAA39699 (PubMed:2046655).Curated
Sequence conflicti259 – 2591R → K in CAA39699 (PubMed:2046655).Curated
Sequence conflicti430 – 4323ILV → VLA in CAA39699 (PubMed:2046655).Curated
Sequence conflicti591 – 5911N → H in CAA39699 (PubMed:2046655).Curated

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
J04186 Genomic DNA. Translation: AAA66916.1.
X56259 Genomic DNA. Translation: CAA39699.1.
Z68196 Genomic DNA. Translation: CAA92376.1.
Z74333 Genomic DNA. Translation: CAA98863.1.
BK006938 Genomic DNA. Translation: DAA11885.1.
PIRiA92695. SYBYKT.
RefSeqiNP_010322.1. NM_001180345.1.

Genome annotation databases

EnsemblFungiiYDR037W; YDR037W; YDR037W.
GeneIDi851607.
KEGGisce:YDR037W.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
J04186 Genomic DNA. Translation: AAA66916.1.
X56259 Genomic DNA. Translation: CAA39699.1.
Z68196 Genomic DNA. Translation: CAA92376.1.
Z74333 Genomic DNA. Translation: CAA98863.1.
BK006938 Genomic DNA. Translation: DAA11885.1.
PIRiA92695. SYBYKT.
RefSeqiNP_010322.1. NM_001180345.1.

3D structure databases

ProteinModelPortaliP15180.
SMRiP15180. Positions 69-579.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi32092. 91 interactions.
DIPiDIP-6359N.
IntActiP15180. 7 interactions.
MINTiMINT-644811.

PTM databases

iPTMnetiP15180.

Proteomic databases

MaxQBiP15180.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblFungiiYDR037W; YDR037W; YDR037W.
GeneIDi851607.
KEGGisce:YDR037W.

Organism-specific databases

EuPathDBiFungiDB:YDR037W.
SGDiS000002444. KRS1.

Phylogenomic databases

GeneTreeiENSGT00550000074841.
HOGENOMiHOG000236577.
InParanoidiP15180.
KOiK04567.
OMAiHHNTLDI.
OrthoDBiEOG092C0ZU5.

Enzyme and pathway databases

BioCyciYEAST:G3O-29651-MONOMER.

Miscellaneous databases

PROiP15180.

Family and domain databases

CDDicd00775. LysRS_core. 1 hit.
Gene3Di2.40.50.140. 1 hit.
HAMAPiMF_00252. Lys_tRNA_synth_class2. 1 hit.
InterProiIPR004364. aa-tRNA-synt_II.
IPR018150. aa-tRNA-synt_II-like.
IPR006195. aa-tRNA-synth_II.
IPR002313. Lys-tRNA-ligase_II.
IPR018149. Lys-tRNA-synth_II_C.
IPR012340. NA-bd_OB-fold.
IPR004365. NA-bd_OB_tRNA.
[Graphical view]
PANTHERiPTHR22594. PTHR22594. 1 hit.
PfamiPF00152. tRNA-synt_2. 1 hit.
PF01336. tRNA_anti-codon. 1 hit.
[Graphical view]
PIRSFiPIRSF039101. LysRS2. 1 hit.
PRINTSiPR00982. TRNASYNTHLYS.
SUPFAMiSSF50249. SSF50249. 1 hit.
TIGRFAMsiTIGR00499. lysS_bact. 1 hit.
PROSITEiPS50862. AA_TRNA_LIGASE_II. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiSYKC_YEAST
AccessioniPrimary (citable) accession number: P15180
Secondary accession number(s): D6VS25
Entry historyi
Integrated into UniProtKB/Swiss-Prot: April 1, 1990
Last sequence update: January 23, 2007
Last modified: September 7, 2016
This is version 169 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Direct protein sequencing, Reference proteome

Documents

  1. Aminoacyl-tRNA synthetases
    List of aminoacyl-tRNA synthetase entries
  2. SIMILARITY comments
    Index of protein domains and families
  3. Yeast
    Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD
  4. Yeast chromosome IV
    Yeast (Saccharomyces cerevisiae) chromosome IV: entries and gene names

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.