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Protein

Aspartate--tRNA ligase, mitochondrial

Gene

MSD1

Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Experimental evidence at protein leveli

Functioni

Catalytic activityi

ATP + L-aspartate + tRNA(Asp) = AMP + diphosphate + L-aspartyl-tRNA(Asp).

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei198 – 1981AspartateBy similarity
Binding sitei248 – 2481AspartateBy similarity
Binding sitei553 – 5531ATPBy similarity
Binding sitei560 – 5601AspartateBy similarity

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Nucleotide bindingi248 – 2503ATPBy similarity
Nucleotide bindingi604 – 6074ATPBy similarity

GO - Molecular functioni

  • aspartate-tRNA ligase activity Source: SGD
  • ATP binding Source: UniProtKB-KW
  • nucleic acid binding Source: InterPro

GO - Biological processi

  • mitochondrial aspartyl-tRNA aminoacylation Source: SGD
Complete GO annotation...

Keywords - Molecular functioni

Aminoacyl-tRNA synthetase, Ligase

Keywords - Biological processi

Protein biosynthesis

Keywords - Ligandi

ATP-binding, Nucleotide-binding

Enzyme and pathway databases

BioCyciYEAST:G3O-34006-MONOMER.
SABIO-RKP15179.

Names & Taxonomyi

Protein namesi
Recommended name:
Aspartate--tRNA ligase, mitochondrial (EC:6.1.1.12)
Alternative name(s):
Aspartyl-tRNA synthetase
Short name:
AspRS
Gene namesi
Name:MSD1
Ordered Locus Names:YPL104W
ORF Names:LPG5W
OrganismiSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Taxonomic identifieri559292 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces
Proteomesi
  • UP000002311 Componenti: Chromosome XVI

Organism-specific databases

EuPathDBiFungiDB:YPL104W.
SGDiS000006025. MSD1.

Subcellular locationi

GO - Cellular componenti

  • mitochondrial matrix Source: UniProtKB-SubCell
  • mitochondrion Source: SGD
Complete GO annotation...

Keywords - Cellular componenti

Mitochondrion

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 658658Aspartate--tRNA ligase, mitochondrialPRO_0000111015Add
BLAST

Proteomic databases

MaxQBiP15179.
PRIDEiP15179.

Interactioni

Protein-protein interaction databases

BioGridi36077. 63 interactions.
DIPiDIP-6339N.
IntActiP15179. 4 interactions.
MINTiMINT-688158.

Structurei

3D structure databases

ProteinModelPortaliP15179.
SMRiP15179. Positions 35-650.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni226 – 2294AspartateBy similarity

Sequence similaritiesi

Phylogenomic databases

GeneTreeiENSGT00550000074971.
HOGENOMiHOG000275159.
InParanoidiP15179.
KOiK01876.
OMAiSRRIHNA.
OrthoDBiEOG71K6BJ.

Family and domain databases

Gene3Di2.40.50.140. 1 hit.
3.30.1360.30. 1 hit.
InterProiIPR004364. aa-tRNA-synt_II.
IPR018150. aa-tRNA-synt_II-like.
IPR006195. aa-tRNA-synth_II.
IPR004524. Asp-tRNA-ligase_bac/mit.
IPR002312. Asp/Asn-tRNA-synth_IIb.
IPR004115. GAD-like.
IPR012340. NA-bd_OB-fold.
IPR004365. NA-bd_OB_tRNA.
[Graphical view]
PANTHERiPTHR22594. PTHR22594. 3 hits.
PTHR22594:SF5. PTHR22594:SF5. 3 hits.
PfamiPF00152. tRNA-synt_2. 1 hit.
PF01336. tRNA_anti-codon. 1 hit.
[Graphical view]
PRINTSiPR01042. TRNASYNTHASP.
SUPFAMiSSF50249. SSF50249. 1 hit.
TIGRFAMsiTIGR00459. aspS_bact. 1 hit.
PROSITEiPS50862. AA_TRNA_LIGASE_II. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

P15179-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MLARSRVCLQ TITRRLADFP EANAIKKKFL FRKDTSTIKQ LKGLSSGQKI
60 70 80 90 100
VLNGWIEQKP KRVGKNLIFG LLRDSNGDII QLVDNKSLLK GFTLEDVVQA
110 120 130 140 150
VGILSLKRKL SNEDADEYEV QLEDITVLNA SNKKPAQMQD FKLSAIYPPE
160 170 180 190 200
FRYLQLRNPK YQDFLKKRSS ISKEIRNSFN NFDFTEVETP MLFKATPEGA
210 220 230 240 250
REFLVPTRTK RSDGKPSFYA LDQSPQQYKQ LLMASGVNKY YQMARCFRDE
260 270 280 290 300
DLRADRQPEF TQVDMEMAFA NSEDVMKIIE KTVSGVWSKF SKKRGLLTLD
310 320 330 340 350
SKGTLVPAKK ENGTVSIFRM TYEQAMTSYG IDKPDLRAPD LKIINLGEFN
360 370 380 390 400
AFSHLNKKFP VFEVIILRSA FSNMEEYKER WSFLTNNSNY NYRVPIVLPI
410 420 430 440 450
ENDEQANSNW FENFHAIATF ENPHLITKFL KLKKGDIVCG CTREPNHSIF
460 470 480 490 500
ENPTPLGRLR QLVLQSEHGK NIYHAVNKDV ASWIVDFPLF SPVIIEDKSG
510 520 530 540 550
KKEKLAYPEY EKDRLCSTHH PFTMVKLKDY EKLEKTPEKC LGRHYDLVVN
560 570 580 590 600
GVELGGGSTR IHDPRLQDYI FEDILKIDNA YELFGHLLNA FDMGTPPHAG
610 620 630 640 650
FAIGFDRMCA MICETESIRD VIAFPKSITG ADLVVKSPSV IPESILEPYN

IKYSNSKK
Length:658
Mass (Da):75,461
Last modified:April 1, 1990 - v1
Checksum:i664081ED6DE24964
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
M26020 Genomic DNA. Translation: AAA35137.1.
M24418 Genomic DNA. Translation: AAA35173.1.
U43281 Genomic DNA. Translation: AAB68196.1.
BK006949 Genomic DNA. Translation: DAA11329.1.
PIRiS61963. SYBYDM.
RefSeqiNP_015221.1. NM_001183918.1.

Genome annotation databases

EnsemblFungiiYPL104W; YPL104W; YPL104W.
GeneIDi856000.
KEGGisce:YPL104W.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
M26020 Genomic DNA. Translation: AAA35137.1.
M24418 Genomic DNA. Translation: AAA35173.1.
U43281 Genomic DNA. Translation: AAB68196.1.
BK006949 Genomic DNA. Translation: DAA11329.1.
PIRiS61963. SYBYDM.
RefSeqiNP_015221.1. NM_001183918.1.

3D structure databases

ProteinModelPortaliP15179.
SMRiP15179. Positions 35-650.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi36077. 63 interactions.
DIPiDIP-6339N.
IntActiP15179. 4 interactions.
MINTiMINT-688158.

Proteomic databases

MaxQBiP15179.
PRIDEiP15179.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblFungiiYPL104W; YPL104W; YPL104W.
GeneIDi856000.
KEGGisce:YPL104W.

Organism-specific databases

EuPathDBiFungiDB:YPL104W.
SGDiS000006025. MSD1.

Phylogenomic databases

GeneTreeiENSGT00550000074971.
HOGENOMiHOG000275159.
InParanoidiP15179.
KOiK01876.
OMAiSRRIHNA.
OrthoDBiEOG71K6BJ.

Enzyme and pathway databases

BioCyciYEAST:G3O-34006-MONOMER.
SABIO-RKP15179.

Miscellaneous databases

NextBioi980869.
PROiP15179.

Family and domain databases

Gene3Di2.40.50.140. 1 hit.
3.30.1360.30. 1 hit.
InterProiIPR004364. aa-tRNA-synt_II.
IPR018150. aa-tRNA-synt_II-like.
IPR006195. aa-tRNA-synth_II.
IPR004524. Asp-tRNA-ligase_bac/mit.
IPR002312. Asp/Asn-tRNA-synth_IIb.
IPR004115. GAD-like.
IPR012340. NA-bd_OB-fold.
IPR004365. NA-bd_OB_tRNA.
[Graphical view]
PANTHERiPTHR22594. PTHR22594. 3 hits.
PTHR22594:SF5. PTHR22594:SF5. 3 hits.
PfamiPF00152. tRNA-synt_2. 1 hit.
PF01336. tRNA_anti-codon. 1 hit.
[Graphical view]
PRINTSiPR01042. TRNASYNTHASP.
SUPFAMiSSF50249. SSF50249. 1 hit.
TIGRFAMsiTIGR00459. aspS_bact. 1 hit.
PROSITEiPS50862. AA_TRNA_LIGASE_II. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. "Homology of aspartyl- and lysyl-tRNA synthetases."
    Gampel A., Tzagoloff A.
    Proc. Natl. Acad. Sci. U.S.A. 86:6023-6027(1989) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
  2. "The nucleotide sequence of Saccharomyces cerevisiae chromosome XVI."
    Bussey H., Storms R.K., Ahmed A., Albermann K., Allen E., Ansorge W., Araujo R., Aparicio A., Barrell B.G., Badcock K., Benes V., Botstein D., Bowman S., Brueckner M., Carpenter J., Cherry J.M., Chung E., Churcher C.M.
    , Coster F., Davis K., Davis R.W., Dietrich F.S., Delius H., DiPaolo T., Dubois E., Duesterhoeft A., Duncan M., Floeth M., Fortin N., Friesen J.D., Fritz C., Goffeau A., Hall J., Hebling U., Heumann K., Hilbert H., Hillier L.W., Hunicke-Smith S., Hyman R.W., Johnston M., Kalman S., Kleine K., Komp C., Kurdi O., Lashkari D., Lew H., Lin A., Lin D., Louis E.J., Marathe R., Messenguy F., Mewes H.-W., Mirtipati S., Moestl D., Mueller-Auer S., Namath A., Nentwich U., Oefner P., Pearson D., Petel F.X., Pohl T.M., Purnelle B., Rajandream M.A., Rechmann S., Rieger M., Riles L., Roberts D., Schaefer M., Scharfe M., Scherens B., Schramm S., Schroeder M., Sdicu A.-M., Tettelin H., Urrestarazu L.A., Ushinsky S., Vierendeels F., Vissers S., Voss H., Walsh S.V., Wambutt R., Wang Y., Wedler E., Wedler H., Winnett E., Zhong W.-W., Zollner A., Vo D.H., Hani J.
    Nature 387:103-105(1997) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: ATCC 204508 / S288c.
  3. Cited for: GENOME REANNOTATION.
    Strain: ATCC 204508 / S288c.
  4. Cited for: LEVEL OF PROTEIN EXPRESSION [LARGE SCALE ANALYSIS].

Entry informationi

Entry nameiSYDM_YEAST
AccessioniPrimary (citable) accession number: P15179
Secondary accession number(s): D6W3R3
Entry historyi
Integrated into UniProtKB/Swiss-Prot: April 1, 1990
Last sequence update: April 1, 1990
Last modified: May 11, 2016
This is version 148 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Miscellaneous

Present with 1550 molecules/cell in log phase SD medium.1 Publication

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Aminoacyl-tRNA synthetases
    List of aminoacyl-tRNA synthetase entries
  2. SIMILARITY comments
    Index of protein domains and families
  3. Yeast
    Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD
  4. Yeast chromosome XVI
    Yeast (Saccharomyces cerevisiae) chromosome XVI: entries and gene names

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.