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Protein

Aspartate--tRNA ligase, mitochondrial

Gene

MSD1

Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Miscellaneous

Present with 1550 molecules/cell in log phase SD medium.1 Publication

Catalytic activityi

ATP + L-aspartate + tRNA(Asp) = AMP + diphosphate + L-aspartyl-tRNA(Asp).

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Binding sitei198AspartateBy similarity1
Binding sitei248AspartateBy similarity1
Binding sitei553ATPBy similarity1
Binding sitei560AspartateBy similarity1

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Nucleotide bindingi248 – 250ATPBy similarity3
Nucleotide bindingi604 – 607ATPBy similarity4

GO - Molecular functioni

  • aspartate-tRNA ligase activity Source: SGD
  • ATP binding Source: UniProtKB-KW
  • nucleic acid binding Source: InterPro

GO - Biological processi

  • mitochondrial aspartyl-tRNA aminoacylation Source: SGD

Keywordsi

Molecular functionAminoacyl-tRNA synthetase, Ligase
Biological processProtein biosynthesis
LigandATP-binding, Nucleotide-binding

Enzyme and pathway databases

BioCyciYEAST:G3O-34006-MONOMER
SABIO-RKiP15179

Names & Taxonomyi

Protein namesi
Recommended name:
Aspartate--tRNA ligase, mitochondrial (EC:6.1.1.12)
Alternative name(s):
Aspartyl-tRNA synthetase
Short name:
AspRS
Gene namesi
Name:MSD1
Ordered Locus Names:YPL104W
ORF Names:LPG5W
OrganismiSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Taxonomic identifieri559292 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces
Proteomesi
  • UP000002311 Componenti: Chromosome XVI

Organism-specific databases

EuPathDBiFungiDB:YPL104W
SGDiS000006025 MSD1

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cell wall Cytoskeleton Vacuole Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Mitochondrion

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00001110151 – 658Aspartate--tRNA ligase, mitochondrialAdd BLAST658

Proteomic databases

MaxQBiP15179
PaxDbiP15179
PRIDEiP15179

Interactioni

Protein-protein interaction databases

BioGridi3607779 interactors.
DIPiDIP-6339N
IntActiP15179 12 interactors.
STRINGi4932.YPL104W

Structurei

3D structure databases

ProteinModelPortaliP15179
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni226 – 229AspartateBy similarity4

Sequence similaritiesi

Phylogenomic databases

GeneTreeiENSGT00550000074971
HOGENOMiHOG000275159
InParanoidiP15179
KOiK01876
OMAiYQLDVEM
OrthoDBiEOG092C13C7

Family and domain databases

Gene3Di3.30.1360.301 hit
HAMAPiMF_00044 Asp_tRNA_synth_type1, 1 hit
InterProiView protein in InterPro
IPR004364 aa-tRNA-synt_II
IPR006195 aa-tRNA-synth_II
IPR004524 Asp-tRNA-ligase_1
IPR002312 Asp/Asn-tRNA-synth_IIb
IPR004115 GAD-like_sf
IPR012340 NA-bd_OB-fold
IPR004365 NA-bd_OB_tRNA
PANTHERiPTHR22594:SF5 PTHR22594:SF5, 1 hit
PfamiView protein in Pfam
PF00152 tRNA-synt_2, 1 hit
PF01336 tRNA_anti-codon, 1 hit
PRINTSiPR01042 TRNASYNTHASP
SUPFAMiSSF50249 SSF50249, 1 hit
TIGRFAMsiTIGR00459 aspS_bact, 1 hit
PROSITEiView protein in PROSITE
PS50862 AA_TRNA_LIGASE_II, 1 hit

Sequencei

Sequence statusi: Complete.

P15179-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MLARSRVCLQ TITRRLADFP EANAIKKKFL FRKDTSTIKQ LKGLSSGQKI
60 70 80 90 100
VLNGWIEQKP KRVGKNLIFG LLRDSNGDII QLVDNKSLLK GFTLEDVVQA
110 120 130 140 150
VGILSLKRKL SNEDADEYEV QLEDITVLNA SNKKPAQMQD FKLSAIYPPE
160 170 180 190 200
FRYLQLRNPK YQDFLKKRSS ISKEIRNSFN NFDFTEVETP MLFKATPEGA
210 220 230 240 250
REFLVPTRTK RSDGKPSFYA LDQSPQQYKQ LLMASGVNKY YQMARCFRDE
260 270 280 290 300
DLRADRQPEF TQVDMEMAFA NSEDVMKIIE KTVSGVWSKF SKKRGLLTLD
310 320 330 340 350
SKGTLVPAKK ENGTVSIFRM TYEQAMTSYG IDKPDLRAPD LKIINLGEFN
360 370 380 390 400
AFSHLNKKFP VFEVIILRSA FSNMEEYKER WSFLTNNSNY NYRVPIVLPI
410 420 430 440 450
ENDEQANSNW FENFHAIATF ENPHLITKFL KLKKGDIVCG CTREPNHSIF
460 470 480 490 500
ENPTPLGRLR QLVLQSEHGK NIYHAVNKDV ASWIVDFPLF SPVIIEDKSG
510 520 530 540 550
KKEKLAYPEY EKDRLCSTHH PFTMVKLKDY EKLEKTPEKC LGRHYDLVVN
560 570 580 590 600
GVELGGGSTR IHDPRLQDYI FEDILKIDNA YELFGHLLNA FDMGTPPHAG
610 620 630 640 650
FAIGFDRMCA MICETESIRD VIAFPKSITG ADLVVKSPSV IPESILEPYN

IKYSNSKK
Length:658
Mass (Da):75,461
Last modified:April 1, 1990 - v1
Checksum:i664081ED6DE24964
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
M26020 Genomic DNA Translation: AAA35137.1
M24418 Genomic DNA Translation: AAA35173.1
U43281 Genomic DNA Translation: AAB68196.1
BK006949 Genomic DNA Translation: DAA11329.1
PIRiS61963 SYBYDM
RefSeqiNP_015221.1, NM_001183918.1

Genome annotation databases

EnsemblFungiiYPL104W; YPL104W; YPL104W
GeneIDi856000
KEGGisce:YPL104W

Similar proteinsi

Entry informationi

Entry nameiSYDM_YEAST
AccessioniPrimary (citable) accession number: P15179
Secondary accession number(s): D6W3R3
Entry historyiIntegrated into UniProtKB/Swiss-Prot: April 1, 1990
Last sequence update: April 1, 1990
Last modified: March 28, 2018
This is version 166 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome