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Protein

Aspartate--tRNA ligase, cytoplasmic

Gene

Dars

Organism
Rattus norvegicus (Rat)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Experimental evidence at transcript leveli

Functioni

Catalyzes the specific attachment of an amino acid to its cognate tRNA in a 2 step reaction: the amino acid (AA) is first activated by ATP to form AA-AMP and then transferred to the acceptor end of the tRNA.

Catalytic activityi

ATP + L-aspartate + tRNA(Asp) = AMP + diphosphate + L-aspartyl-tRNA(Asp).

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei229 – 2291AspartateBy similarity
Binding sitei273 – 2731AspartateBy similarity
Binding sitei424 – 4241ATPBy similarity
Binding sitei427 – 4271AspartateBy similarity
Binding sitei431 – 4311AspartateBy similarity

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Nucleotide bindingi273 – 2753ATPBy similarity
Nucleotide bindingi281 – 2833ATPBy similarity
Nucleotide bindingi472 – 4754ATPBy similarity

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Aminoacyl-tRNA synthetase, Ligase

Keywords - Biological processi

Protein biosynthesis

Keywords - Ligandi

ATP-binding, Nucleotide-binding

Names & Taxonomyi

Protein namesi
Recommended name:
Aspartate--tRNA ligase, cytoplasmic (EC:6.1.1.12)
Alternative name(s):
Aspartyl-tRNA synthetase
Short name:
AspRS
Gene namesi
Name:Dars
Synonyms:Drs1
OrganismiRattus norvegicus (Rat)
Taxonomic identifieri10116 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeRattus
Proteomesi
  • UP000002494 Componenti: Chromosome 13

Organism-specific databases

RGDi621167. Dars.

Subcellular locationi

GO - Cellular componenti

  • aminoacyl-tRNA synthetase multienzyme complex Source: RGD
  • cytoplasm Source: UniProtKB-SubCell
  • extracellular exosome Source: Ensembl
  • membrane Source: Ensembl
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 501501Aspartate--tRNA ligase, cytoplasmicPRO_0000111012Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei52 – 521PhosphothreonineBy similarity
Modified residuei74 – 741N6-acetyllysineBy similarity
Modified residuei249 – 2491PhosphoserineBy similarity
Modified residuei374 – 3741N6-acetyllysineBy similarity

Keywords - PTMi

Acetylation, Phosphoprotein

Proteomic databases

PaxDbiP15178.
PRIDEiP15178.

PTM databases

iPTMnetiP15178.
PhosphoSiteiP15178.

Expressioni

Gene expression databases

BgeeiENSRNOG00000003743.
ExpressionAtlasiP15178. baseline and differential.
GenevisibleiP15178. RN.

Interactioni

Subunit structurei

Homodimer; also part of a multisubunit complex that groups AIMP1, AIMP2, EEF1A1 and tRNA ligases for Arg, Asp, Glu, Gln, Ile, Leu, Lys, Met and Pro.

Protein-protein interaction databases

BioGridi250460. 1 interaction.
IntActiP15178. 1 interaction.
STRINGi10116.ENSRNOP00000005127.

Structurei

3D structure databases

ProteinModelPortaliP15178.
SMRiP15178. Positions 22-501.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni251 – 2544AspartateBy similarity

Sequence similaritiesi

Phylogenomic databases

eggNOGiKOG0556. Eukaryota.
COG0017. LUCA.
GeneTreeiENSGT00550000074880.
HOGENOMiHOG000226032.
HOVERGENiHBG001028.
InParanoidiP15178.
KOiK01876.
OMAiWGDDLST.
OrthoDBiEOG091G0530.
PhylomeDBiP15178.
TreeFamiTF105676.

Family and domain databases

Gene3Di2.40.50.140. 1 hit.
InterProiIPR004364. aa-tRNA-synt_II.
IPR018150. aa-tRNA-synt_II-like.
IPR006195. aa-tRNA-synth_II.
IPR004523. Asp-tRNA_synthase_arc.
IPR002312. Asp/Asn-tRNA-synth_IIb.
IPR012340. NA-bd_OB-fold.
IPR004365. NA-bd_OB_tRNA.
[Graphical view]
PANTHERiPTHR22594. PTHR22594. 1 hit.
PTHR22594:SF33. PTHR22594:SF33. 1 hit.
PfamiPF00152. tRNA-synt_2. 1 hit.
PF01336. tRNA_anti-codon. 1 hit.
[Graphical view]
PRINTSiPR01042. TRNASYNTHASP.
SUPFAMiSSF50249. SSF50249. 1 hit.
TIGRFAMsiTIGR00458. aspS_nondisc. 1 hit.
PROSITEiPS50862. AA_TRNA_LIGASE_II. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

P15178-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MPSANASRKG QEKPREIVDA AEDYAKERYG VSSMIQSQEK PDRVLVRVKD
60 70 80 90 100
LTVQKADEVV WVRARVHTSR AKGKQCFLVL RQQQFNVQAL VAVGDHASKQ
110 120 130 140 150
MVKFAANINK ESIIDVEGIV RKVNQKIGSC TQQDVELHVQ KIYVISLAEP
160 170 180 190 200
RLPLQLDDAI RPEVEGEEDG RATVNQDTRL DNRIIDLRTS TSQAIFHLQS
210 220 230 240 250
GICHLFRETL INKGFVEIQT PKIISAASEG GANVFTVSYF KSNAYLAQSP
260 270 280 290 300
QLYKQMCICA DFEKVFCIGP VFRAEDSNTH RHLTEFVGLD IEMAFNYHYH
310 320 330 340 350
EVVEEIADTL VQIFKGLQER FQTEIQTVNK QFPCEPFKFL EPTLRLEYCE
360 370 380 390 400
ALAMLREAGV EMDDEEDLST PNEKLLGRLV KEKYDTDFYV LDKYPLAVRP
410 420 430 440 450
FYTMPDPRNP KQSNSYDMFM RGEEILSGAQ RIHDPQLLTE RALHHGIDLE
460 470 480 490 500
KIKAYIDSFR FGAPPHAGGG IGLERVTMLF LGLHNVRQTS MFPRDPKRLT

P
Length:501
Mass (Da):57,126
Last modified:April 1, 1990 - v1
Checksum:iD3D389B39D7327E2
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
J04487 mRNA. Translation: AAA40789.1.
U30812
, U30485, U30800, U30801, U30802, U30803, U30804, U30805, U30806, U30807, U30808, U30809, U30810, U30811 Genomic DNA. Translation: AAC52981.1.
BC072534 mRNA. Translation: AAH72534.1.
PIRiA32197. SYRTDT.
RefSeqiNP_446251.1. NM_053799.3.
UniGeneiRn.2388.

Genome annotation databases

EnsembliENSRNOT00000005127; ENSRNOP00000005127; ENSRNOG00000003743.
GeneIDi116483.
KEGGirno:116483.
UCSCiRGD:621167. rat.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
J04487 mRNA. Translation: AAA40789.1.
U30812
, U30485, U30800, U30801, U30802, U30803, U30804, U30805, U30806, U30807, U30808, U30809, U30810, U30811 Genomic DNA. Translation: AAC52981.1.
BC072534 mRNA. Translation: AAH72534.1.
PIRiA32197. SYRTDT.
RefSeqiNP_446251.1. NM_053799.3.
UniGeneiRn.2388.

3D structure databases

ProteinModelPortaliP15178.
SMRiP15178. Positions 22-501.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi250460. 1 interaction.
IntActiP15178. 1 interaction.
STRINGi10116.ENSRNOP00000005127.

PTM databases

iPTMnetiP15178.
PhosphoSiteiP15178.

Proteomic databases

PaxDbiP15178.
PRIDEiP15178.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSRNOT00000005127; ENSRNOP00000005127; ENSRNOG00000003743.
GeneIDi116483.
KEGGirno:116483.
UCSCiRGD:621167. rat.

Organism-specific databases

CTDi1615.
RGDi621167. Dars.

Phylogenomic databases

eggNOGiKOG0556. Eukaryota.
COG0017. LUCA.
GeneTreeiENSGT00550000074880.
HOGENOMiHOG000226032.
HOVERGENiHBG001028.
InParanoidiP15178.
KOiK01876.
OMAiWGDDLST.
OrthoDBiEOG091G0530.
PhylomeDBiP15178.
TreeFamiTF105676.

Miscellaneous databases

PROiP15178.

Gene expression databases

BgeeiENSRNOG00000003743.
ExpressionAtlasiP15178. baseline and differential.
GenevisibleiP15178. RN.

Family and domain databases

Gene3Di2.40.50.140. 1 hit.
InterProiIPR004364. aa-tRNA-synt_II.
IPR018150. aa-tRNA-synt_II-like.
IPR006195. aa-tRNA-synth_II.
IPR004523. Asp-tRNA_synthase_arc.
IPR002312. Asp/Asn-tRNA-synth_IIb.
IPR012340. NA-bd_OB-fold.
IPR004365. NA-bd_OB_tRNA.
[Graphical view]
PANTHERiPTHR22594. PTHR22594. 1 hit.
PTHR22594:SF33. PTHR22594:SF33. 1 hit.
PfamiPF00152. tRNA-synt_2. 1 hit.
PF01336. tRNA_anti-codon. 1 hit.
[Graphical view]
PRINTSiPR01042. TRNASYNTHASP.
SUPFAMiSSF50249. SSF50249. 1 hit.
TIGRFAMsiTIGR00458. aspS_nondisc. 1 hit.
PROSITEiPS50862. AA_TRNA_LIGASE_II. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiSYDC_RAT
AccessioniPrimary (citable) accession number: P15178
Entry historyi
Integrated into UniProtKB/Swiss-Prot: April 1, 1990
Last sequence update: April 1, 1990
Last modified: September 7, 2016
This is version 133 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Aminoacyl-tRNA synthetases
    List of aminoacyl-tRNA synthetase entries
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.