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P15173

- MYOG_HUMAN

UniProt

P15173 - MYOG_HUMAN

Protein

Myogenin

Gene

MYOG

Organism
Homo sapiens (Human)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at transcript leveli
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    • History
      Entry version 136 (01 Oct 2014)
      Sequence version 2 (01 Dec 1992)
      Previous versions | rss
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    Functioni

    Acts as a transcriptional activator that promotes transcription of muscle-specific target genes and plays a role in muscle differentiation, cell cycle exit and muscle atrophy. Essential for the development of functional embryonic skeletal fiber muscle differentiation. However is dispensable for postnatal skeletal muscle growth; phosphorylation by CAMK2G inhibits its transcriptional activity in respons to muscle activity. Required for the recruitment of the FACT complex to muscle-specific promoter regions, thus promoting gene expression initiation. During terminal myoblast differentiation, plays a role as a strong activator of transcription at loci with an open chromatin structure previously initiated by MYOD1. Together with MYF5 and MYOD1, co-occupies muscle-specific gene promoter core regions during myogenesis. Cooperates also with myocyte-specific enhancer factor MEF2D and BRG1-dependent recruitment of SWI/SNF chromatin-remodeling enzymes to alter chromatin structure at myogenic late gene promoters. Facilitates cell cycle exit during terminal muscle differentiation through the up-regulation of miR-20a expression, which in turn represses genes involved in cell cycle progression. Binds to the E-box containing (E1) promoter region of the miR-20a gene. Plays also a role in preventing reversal of muscle cell differentiation. Contributes to the atrophy-related gene expression in adult denervated muscles. Induces fibroblasts to differentiate into myoblasts By similarity.By similarity

    GO - Molecular functioni

    1. chromatin DNA binding Source: UniProtKB
    2. core promoter binding Source: UniProtKB
    3. core promoter proximal region sequence-specific DNA binding Source: Ensembl
    4. E-box binding Source: BHF-UCL
    5. protein heterodimerization activity Source: BHF-UCL
    6. RNA polymerase II regulatory region sequence-specific DNA binding Source: NTNU_SB
    7. RNA polymerase II transcription regulatory region sequence-specific DNA binding transcription factor activity involved in positive regulation of transcription Source: NTNU_SB
    8. sequence-specific DNA binding Source: NTNU_SB
    9. sequence-specific DNA binding transcription factor activity Source: UniProtKB

    GO - Biological processi

    1. cell cycle Source: UniProtKB-KW
    2. cellular response to estradiol stimulus Source: UniProtKB
    3. cellular response to growth factor stimulus Source: Ensembl
    4. cellular response to lithium ion Source: Ensembl
    5. mRNA transcription from RNA polymerase II promoter Source: BHF-UCL
    6. muscle cell differentiation Source: Reactome
    7. muscle cell fate commitment Source: BHF-UCL
    8. negative regulation of cell proliferation Source: UniProtKB
    9. ossification Source: Ensembl
    10. positive regulation of cell cycle arrest Source: UniProtKB
    11. positive regulation of muscle atrophy Source: UniProtKB
    12. positive regulation of muscle cell differentiation Source: Reactome
    13. positive regulation of myoblast differentiation Source: UniProtKB
    14. positive regulation of myotube differentiation Source: UniProtKB
    15. positive regulation of skeletal muscle fiber development Source: UniProtKB
    16. positive regulation of transcription from RNA polymerase II promoter Source: UniProtKB
    17. regulation of myoblast fusion Source: UniProtKB
    18. regulation of satellite cell proliferation Source: UniProtKB
    19. response to denervation involved in regulation of muscle adaptation Source: UniProtKB
    20. response to electrical stimulus involved in regulation of muscle adaptation Source: UniProtKB
    21. response to muscle activity involved in regulation of muscle adaptation Source: UniProtKB
    22. skeletal muscle fiber development Source: Ensembl
    23. skeletal muscle tissue development Source: ProtInc
    24. striated muscle atrophy Source: UniProtKB

    Keywords - Molecular functioni

    Activator, Developmental protein

    Keywords - Biological processi

    Cell cycle, Differentiation, Myogenesis, Transcription, Transcription regulation

    Keywords - Ligandi

    DNA-binding

    Enzyme and pathway databases

    ReactomeiREACT_21402. CDO in myogenesis.
    SignaLinkiP15173.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Myogenin
    Alternative name(s):
    Class C basic helix-loop-helix protein 3
    Short name:
    bHLHc3
    Myogenic factor 4
    Short name:
    Myf-4
    Gene namesi
    Name:MYOG
    Synonyms:BHLHC3, MYF4
    OrganismiHomo sapiens (Human)
    Taxonomic identifieri9606 [NCBI]
    Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo
    ProteomesiUP000005640: Chromosome 1

    Organism-specific databases

    HGNCiHGNC:7612. MYOG.

    Subcellular locationi

    Nucleus
    Note: Recruited to late myogenic gene promoter regulatory sequences with SMARCA4/BRG1/BAF190A and SWI/SNF chromatin-remodeling enzymes to promote chromatin-remodeling and transcription initiation in developing embryos.By similarity

    GO - Cellular componenti

    1. nucleoplasm Source: Reactome
    2. nucleus Source: UniProtKB
    3. protein-DNA complex Source: UniProtKB
    4. transcription factor complex Source: BHF-UCL

    Keywords - Cellular componenti

    Nucleus

    Pathology & Biotechi

    Organism-specific databases

    PharmGKBiPA31417.

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 224224MyogeninPRO_0000127375Add
    BLAST

    Amino acid modifications

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Modified residuei77 – 771Phosphoserine; by CaMK2GBy similarity
    Modified residuei79 – 791Phosphoserine; by CaMK2GBy similarity
    Modified residuei87 – 871Phosphothreonine; by CaMK2GBy similarity

    Post-translational modificationi

    Phosphorylated by CAMK2G on threonine and serine amino acids in a muscle activity-dependent manner. Phosphorylation of Thr-87 impairs both DNA-binding and trans-activation functions in contracting muscles By similarity.By similarity

    Keywords - PTMi

    Phosphoprotein

    Proteomic databases

    PaxDbiP15173.
    PRIDEiP15173.

    PTM databases

    PhosphoSiteiP15173.

    Expressioni

    Gene expression databases

    BgeeiP15173.
    CleanExiHS_MYOG.
    GenevestigatoriP15173.

    Organism-specific databases

    HPAiHPA038093.

    Interactioni

    Subunit structurei

    Homodimer and heterodimer with E12; heterodimerization enhances MYOG DNA-binding and transcriptional activities. Interacts with SMARCA4/BRG1/BAF190A. Interacts (via C-terminal region) with SSRP1 and SUPT16H; the interaction is indicative of an interaction with the FACT complex By similarity.By similarity

    Protein-protein interaction databases

    BioGridi110739. 17 interactions.
    DIPiDIP-159N.
    IntActiP15173. 8 interactions.
    MINTiMINT-1516099.
    STRINGi9606.ENSP00000241651.

    Structurei

    3D structure databases

    ProteinModelPortaliP15173.
    SMRiP15173. Positions 74-136.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Domains and Repeats

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Domaini81 – 13252bHLHPROSITE-ProRule annotationAdd
    BLAST

    Sequence similaritiesi

    Contains 1 bHLH (basic helix-loop-helix) domain.PROSITE-ProRule annotation

    Phylogenomic databases

    eggNOGiNOG285405.
    HOGENOMiHOG000234799.
    HOVERGENiHBG006429.
    InParanoidiP15173.
    OMAiFYQEPHF.
    OrthoDBiEOG76QFK1.
    PhylomeDBiP15173.
    TreeFamiTF316344.

    Family and domain databases

    Gene3Di4.10.280.10. 1 hit.
    InterProiIPR002546. Basic.
    IPR011598. bHLH_dom.
    [Graphical view]
    PfamiPF01586. Basic. 1 hit.
    PF00010. HLH. 1 hit.
    [Graphical view]
    SMARTiSM00520. BASIC. 1 hit.
    SM00353. HLH. 1 hit.
    [Graphical view]
    SUPFAMiSSF47459. SSF47459. 1 hit.
    PROSITEiPS50888. BHLH. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    P15173-1 [UniParc]FASTAAdd to Basket

    « Hide

    MELYETSPYF YQEPRFYDGE NYLPVHLQGF EPPGYERTEL TLSPEAPGPL    50
    EDKGLGTPEH CPGQCLPWAC KVCKRKSVSV DRRRAATLRE KRRLKKVNEA 100
    FEALKRSTLL NPNQRLPKVE ILRSAIQYIE RLQALLSSLN QEERDLRYRG 150
    GGGPQPGVPS ECSSHSASCS PEWGSALEFS ANPGDHLLTA DPTDAHNLHS 200
    LTSIVDSITV EDVSVAFPDE TMPN 224
    Length:224
    Mass (Da):25,037
    Last modified:December 1, 1992 - v2
    Checksum:i91421D69B57551FB
    GO

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    X17651 mRNA. Translation: CAA35641.1. Sequence problems.
    X62155 Genomic DNA. Translation: CAA44080.1.
    BT007233 mRNA. Translation: AAP35897.1.
    CH471067 Genomic DNA. Translation: EAW91463.1.
    BC053899 mRNA. Translation: AAH53899.1.
    CCDSiCCDS1433.1.
    PIRiA41128.
    RefSeqiNP_002470.2. NM_002479.5.
    UniGeneiHs.2830.

    Genome annotation databases

    EnsembliENST00000241651; ENSP00000241651; ENSG00000122180.
    GeneIDi4656.
    KEGGihsa:4656.
    UCSCiuc001gzd.4. human.

    Polymorphism databases

    DMDMi127625.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    X17651 mRNA. Translation: CAA35641.1 . Sequence problems.
    X62155 Genomic DNA. Translation: CAA44080.1 .
    BT007233 mRNA. Translation: AAP35897.1 .
    CH471067 Genomic DNA. Translation: EAW91463.1 .
    BC053899 mRNA. Translation: AAH53899.1 .
    CCDSi CCDS1433.1.
    PIRi A41128.
    RefSeqi NP_002470.2. NM_002479.5.
    UniGenei Hs.2830.

    3D structure databases

    ProteinModelPortali P15173.
    SMRi P15173. Positions 74-136.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    BioGridi 110739. 17 interactions.
    DIPi DIP-159N.
    IntActi P15173. 8 interactions.
    MINTi MINT-1516099.
    STRINGi 9606.ENSP00000241651.

    PTM databases

    PhosphoSitei P15173.

    Polymorphism databases

    DMDMi 127625.

    Proteomic databases

    PaxDbi P15173.
    PRIDEi P15173.

    Protocols and materials databases

    DNASUi 4656.
    Structural Biology Knowledgebase Search...

    Genome annotation databases

    Ensembli ENST00000241651 ; ENSP00000241651 ; ENSG00000122180 .
    GeneIDi 4656.
    KEGGi hsa:4656.
    UCSCi uc001gzd.4. human.

    Organism-specific databases

    CTDi 4656.
    GeneCardsi GC01M203052.
    HGNCi HGNC:7612. MYOG.
    HPAi HPA038093.
    MIMi 159980. gene.
    neXtProti NX_P15173.
    PharmGKBi PA31417.
    GenAtlasi Search...

    Phylogenomic databases

    eggNOGi NOG285405.
    HOGENOMi HOG000234799.
    HOVERGENi HBG006429.
    InParanoidi P15173.
    OMAi FYQEPHF.
    OrthoDBi EOG76QFK1.
    PhylomeDBi P15173.
    TreeFami TF316344.

    Enzyme and pathway databases

    Reactomei REACT_21402. CDO in myogenesis.
    SignaLinki P15173.

    Miscellaneous databases

    ChiTaRSi MYOG. human.
    GeneWikii Myogenin.
    GenomeRNAii 4656.
    NextBioi 17946.
    PROi P15173.
    SOURCEi Search...

    Gene expression databases

    Bgeei P15173.
    CleanExi HS_MYOG.
    Genevestigatori P15173.

    Family and domain databases

    Gene3Di 4.10.280.10. 1 hit.
    InterProi IPR002546. Basic.
    IPR011598. bHLH_dom.
    [Graphical view ]
    Pfami PF01586. Basic. 1 hit.
    PF00010. HLH. 1 hit.
    [Graphical view ]
    SMARTi SM00520. BASIC. 1 hit.
    SM00353. HLH. 1 hit.
    [Graphical view ]
    SUPFAMi SSF47459. SSF47459. 1 hit.
    PROSITEi PS50888. BHLH. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "Differential expression of myogenic determination genes in muscle cells: possible autoactivation by the Myf gene products."
      Braun T., Bober E., Buschhausen-Denker G., Kohtz S., Grzeschik K.-H., Arnold H.H.
      EMBO J. 8:3617-3625(1989) [PubMed] [Europe PMC] [Abstract]
      Cited for: PRELIMINARY NUCLEOTIDE SEQUENCE [MRNA].
      Tissue: Skeletal muscle.
    2. Cited for: SEQUENCE REVISION.
    3. "Transcription of the muscle regulatory gene Myf4 is regulated by serum components, peptide growth factors and signaling pathways involving G proteins."
      Salminen A., Braun T., Buchberger A., Juers S., Winter B., Arnold H.H.
      J. Cell Biol. 115:905-917(1991) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
    4. "Cloning of human full-length CDSs in BD Creator(TM) system donor vector."
      Kalnine N., Chen X., Rolfs A., Halleck A., Hines L., Eisenstein S., Koundinya M., Raphael J., Moreira D., Kelley T., LaBaer J., Lin Y., Phelan M., Farmer A.
      Submitted (MAY-2003) to the EMBL/GenBank/DDBJ databases
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
    5. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    6. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
      The MGC Project Team
      Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
      Tissue: Eye.

    Entry informationi

    Entry nameiMYOG_HUMAN
    AccessioniPrimary (citable) accession number: P15173
    Secondary accession number(s): Q53XW6
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: April 1, 1990
    Last sequence update: December 1, 1992
    Last modified: October 1, 2014
    This is version 136 of the entry and version 2 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programChordata Protein Annotation Program
    DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. Human chromosome 1
      Human chromosome 1: entries, gene names and cross-references to MIM
    2. MIM cross-references
      Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot
    3. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3