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P15170 (ERF3A_HUMAN) Reviewed, UniProtKB/Swiss-Prot

Last modified July 9, 2014. Version 141. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (4) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Interactions·Alt products·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Eukaryotic peptide chain release factor GTP-binding subunit ERF3A

Short name=Eukaryotic peptide chain release factor subunit 3a
Short name=eRF3a
Alternative name(s):
G1 to S phase transition protein 1 homolog
Gene names
Name:GSPT1
Synonyms:ERF3A
OrganismHomo sapiens (Human) [Reference proteome]
Taxonomic identifier9606 [NCBI]
Taxonomic lineageEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo

Protein attributes

Sequence length499 AA.
Sequence statusComplete.
Protein existenceEvidence at protein level

General annotation (Comments)

Function

Involved in translation termination in response to the termination codons UAA, UAG and UGA. Stimulates the activity of ERF1. Involved in regulation of mammalian cell growth. Component of the transient SURF complex which recruits UPF1 to stalled ribosomes in the context of nonsense-mediated decay (NMD) of mRNAs containing premature stop codons.

Subunit structure

Component of the transient SURF (SMG1-UPF1-eRF1-eRF3) complex. Ref.5

Sequence similarities

Belongs to the GTP-binding elongation factor family. ERF3 subfamily.

Caution

eRF3 antibodies used in Ref.5 do not differentiate between GSPT1/ERF3A and GSPT2/ERF3B.

Binary interactions

Alternative products

This entry describes 3 isoforms produced by alternative splicing. [Align] [Select]
Isoform 1 (identifier: P15170-1)

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.
Isoform 2 (identifier: P15170-2)

The sequence of this isoform differs from the canonical sequence as follows:
     1-1: M → MDPGSGGGGG...CEGSNSAVSM
     8-8: Missing.
Note: Ref.4 (AAH09503) sequence is in conflict in position: 6-9:GGGG->G, 92:G->C, 100:V->A.
Isoform 3 (identifier: P15170-3)

The sequence of this isoform differs from the canonical sequence as follows:
     1-1: M → MDPGSGGGGG...CEGSNSAVSM
Note: Gene prediction based on EST data.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 499499Eukaryotic peptide chain release factor GTP-binding subunit ERF3A
PRO_0000091480

Regions

Nucleotide binding81 – 888GTP By similarity
Nucleotide binding158 – 1625GTP By similarity
Nucleotide binding220 – 2234GTP By similarity

Natural variations

Alternative sequence11M → MDPGSGGGGGGGGGGGSSSG SSSSDSAPDCWDQADMEAPG PGPCGGGGSLAAAAEAQREN LSAAFSRQLNVNAKPFVPNV HAAEFVPSFLRGPAAPPPPV GGAANNHGAGSGAGGRAAPV ESSQEEQSLCEGSNSAVSM in isoform 2 and isoform 3.
VSP_042198
Alternative sequence81Missing in isoform 2.
VSP_042199

Secondary structure

................................. 499
Helix Strand Turn

Details...

Sequences

Sequence LengthMass (Da)Tools
Isoform 1 [UniParc].

Last modified April 1, 1990. Version 1.
Checksum: DE20482CCABC3576

FASTA49955,756
        10         20         30         40         50         60 
MELSEPIVEN GETEMSPEES WEHKEEISEA EPGGGSLGDG RPPEESAHEM MEEEEEIPKP 

        70         80         90        100        110        120 
KSVVAPPGAP KKEHVNVVFI GHVDAGKSTI GGQIMYLTGM VDKRTLEKYE REAKEKNRET 

       130        140        150        160        170        180 
WYLSWALDTN QEERDKGKTV EVGRAYFETE KKHFTILDAP GHKSFVPNMI GGASQADLAV 

       190        200        210        220        230        240 
LVISARKGEF ETGFEKGGQT REHAMLAKTA GVKHLIVLIN KMDDPTVNWS NERYEECKEK 

       250        260        270        280        290        300 
LVPFLKKVGF NPKKDIHFMP CSGLTGANLK EQSDFCPWYI GLPFIPYLDN LPNFNRSVDG 

       310        320        330        340        350        360 
PIRLPIVDKY KDMGTVVLGK LESGSICKGQ QLVMMPNKHN VEVLGILSDD VETDTVAPGE 

       370        380        390        400        410        420 
NLKIRLKGIE EEEILPGFIL CDPNNLCHSG RTFDAQIVII EHKSIICPGY NAVLHIHTCI 

       430        440        450        460        470        480 
EEVEITALIC LVDKKSGEKS KTRPRFVKQD QVCIARLRTA GTICLETFKD FPQMGRFTLR 

       490 
DEGKTIAIGK VLKLVPEKD 

« Hide

Isoform 2 [UniParc].

Checksum: 31E3FE3DEDF4BE5E
Show »

FASTA63668,601
Isoform 3 [UniParc].

Checksum: 7765D73A284F35C4
Show »

FASTA63768,700

References

« Hide 'large scale' references
[1]"A human homologue of the yeast GST1 gene codes for a GTP-binding protein and is expressed in a proliferation-dependent manner in mammalian cells."
Hoshino S., Miyazawa H., Enomoto T., Hanaoka F., Kikuchi Y., Kikuchi A., Ui M.
EMBO J. 8:3807-3814(1989) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 1).
[2]"Genome duplications and other features in 12 Mb of DNA sequence from human chromosome 16p and 16q."
Loftus B.J., Kim U.-J., Sneddon V.P., Kalush F., Brandon R., Fuhrmann J., Mason T., Crosby M.L., Barnstead M., Cronin L., Mays A.D., Cao Y., Xu R.X., Kang H.-L., Mitchell S., Eichler E.E., Harris P.C., Venter J.C., Adams M.D.
Genomics 60:295-308(1999) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
[3]"The sequence and analysis of duplication-rich human chromosome 16."
Martin J., Han C., Gordon L.A., Terry A., Prabhakar S., She X., Xie G., Hellsten U., Chan Y.M., Altherr M., Couronne O., Aerts A., Bajorek E., Black S., Blumer H., Branscomb E., Brown N.C., Bruno W.J. expand/collapse author list , Buckingham J.M., Callen D.F., Campbell C.S., Campbell M.L., Campbell E.W., Caoile C., Challacombe J.F., Chasteen L.A., Chertkov O., Chi H.C., Christensen M., Clark L.M., Cohn J.D., Denys M., Detter J.C., Dickson M., Dimitrijevic-Bussod M., Escobar J., Fawcett J.J., Flowers D., Fotopulos D., Glavina T., Gomez M., Gonzales E., Goodstein D., Goodwin L.A., Grady D.L., Grigoriev I., Groza M., Hammon N., Hawkins T., Haydu L., Hildebrand C.E., Huang W., Israni S., Jett J., Jewett P.B., Kadner K., Kimball H., Kobayashi A., Krawczyk M.-C., Leyba T., Longmire J.L., Lopez F., Lou Y., Lowry S., Ludeman T., Manohar C.F., Mark G.A., McMurray K.L., Meincke L.J., Morgan J., Moyzis R.K., Mundt M.O., Munk A.C., Nandkeshwar R.D., Pitluck S., Pollard M., Predki P., Parson-Quintana B., Ramirez L., Rash S., Retterer J., Ricke D.O., Robinson D.L., Rodriguez A., Salamov A., Saunders E.H., Scott D., Shough T., Stallings R.L., Stalvey M., Sutherland R.D., Tapia R., Tesmer J.G., Thayer N., Thompson L.S., Tice H., Torney D.C., Tran-Gyamfi M., Tsai M., Ulanovsky L.E., Ustaszewska A., Vo N., White P.S., Williams A.L., Wills P.L., Wu J.-R., Wu K., Yang J., DeJong P., Bruce D., Doggett N.A., Deaven L., Schmutz J., Grimwood J., Richardson P., Rokhsar D.S., Eichler E.E., Gilna P., Lucas S.M., Myers R.M., Rubin E.M., Pennacchio L.A.
Nature 432:988-994(2004) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
[4]"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
The MGC Project Team
Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 2).
Tissue: Lung.
[5]"SMG-8 and SMG-9, two novel subunits of the SMG-1 complex, regulate remodeling of the mRNA surveillance complex during nonsense-mediated mRNA decay."
Yamashita A., Izumi N., Kashima I., Ohnishi T., Saari B., Katsuhata Y., Muramatsu R., Morita T., Iwamatsu A., Hachiya T., Kurata R., Hirano H., Anderson P., Ohno S.
Genes Dev. 23:1091-1105(2009) [PubMed] [Europe PMC] [Abstract]
Cited for: IDENTIFICATION IN THE SURF COMPLEX.
[6]"Initial characterization of the human central proteome."
Burkard T.R., Planyavsky M., Kaupe I., Breitwieser F.P., Buerckstuemmer T., Bennett K.L., Superti-Furga G., Colinge J.
BMC Syst. Biol. 5:17-17(2011) [PubMed] [Europe PMC] [Abstract]
Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
[7]"Molecular basis of eRF3 recognition by the MLLE domain of poly(A)-binding protein."
Kozlov G., Gehring K.
PLoS ONE 5:E10169-E10169(2010) [PubMed] [Europe PMC] [Abstract]
Cited for: X-RAY CRYSTALLOGRAPHY (1.40 ANGSTROMS) OF 73-87 (ISOFORM 2).
+Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
X17644 mRNA. Translation: CAA35635.1.
U95742 Genomic DNA. Translation: AAB67250.1.
AC007216 Genomic DNA. No translation available.
BC009503 mRNA. Translation: AAH09503.2.
CCDSCCDS45412.1. [P15170-3]
CCDS45413.1. [P15170-2]
CCDS45414.1. [P15170-1]
PIRS06941.
RefSeqNP_001123478.1. NM_001130006.1.
NP_001123479.1. NM_001130007.1. [P15170-1]
UniGeneHs.528780.

3D structure databases

PDBe
RCSB-PDB
PDBj
EntryMethodResolution (Å)ChainPositionsPDBsum
3E1YX-ray3.80E/F/G/H301-499[»]
3J5Yelectron microscopy9.70B69-496[»]
3KUIX-ray2.30B64-78[»]
ProteinModelPortalP15170.
SMRP15170. Positions 2-496.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

BioGrid109190. 28 interactions.
IntActP15170. 8 interactions.
MINTMINT-2859376.
STRING9606.ENSP00000398131.

PTM databases

PhosphoSiteP15170.

Polymorphism databases

DMDM121688.

Proteomic databases

MaxQBP15170.
PaxDbP15170.
PRIDEP15170.

Protocols and materials databases

DNASU2935.
StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblENST00000420576; ENSP00000399539; ENSG00000103342. [P15170-1]
ENST00000434724; ENSP00000398131; ENSG00000103342. [P15170-3]
ENST00000439887; ENSP00000408399; ENSG00000103342. [P15170-2]
ENST00000563468; ENSP00000454351; ENSG00000103342. [P15170-1]
GeneID2935.
KEGGhsa:2935.
UCSCuc002dbu.3. human. [P15170-2]
uc010bux.3. human. [P15170-1]

Organism-specific databases

CTD2935.
GeneCardsGC16M011961.
HGNCHGNC:4621. GSPT1.
HPAHPA052488.
MIM139259. gene.
neXtProtNX_P15170.
PharmGKBPA29012.
GenAtlasSearch...

Phylogenomic databases

eggNOGCOG5256.
HOGENOMHOG000229291.
HOVERGENHBG000179.
InParanoidP15170.
KOK03267.
OMALVVMPNK.
OrthoDBEOG76X5ZT.
PhylomeDBP15170.
TreeFamTF300566.

Gene expression databases

ArrayExpressP15170.
BgeeP15170.
CleanExHS_GSPT1.
GenevestigatorP15170.

Family and domain databases

Gene3D3.40.50.300. 1 hit.
InterProIPR000795. EF_GTP-bd_dom.
IPR027417. P-loop_NTPase.
IPR009000. Transl_B-barrel.
IPR009001. Transl_elong_EF1A/Init_IF2_C.
IPR004161. Transl_elong_EFTu/EF1A_2.
IPR004160. Transl_elong_EFTu/EF1A_C.
[Graphical view]
PfamPF00009. GTP_EFTU. 1 hit.
PF03144. GTP_EFTU_D2. 1 hit.
PF03143. GTP_EFTU_D3. 1 hit.
[Graphical view]
PRINTSPR00315. ELONGATNFCT.
SUPFAMSSF50447. SSF50447. 1 hit.
SSF50465. SSF50465. 1 hit.
SSF52540. SSF52540. 1 hit.
PROSITEPS00301. EFACTOR_GTP. 1 hit.
[Graphical view]
ProtoNetSearch...

Other

ChiTaRSGSPT1. human.
EvolutionaryTraceQ96GF2.
GeneWikiGSPT1.
GenomeRNAi2935.
NextBio11631.
PMAP-CutDBP15170.
PROP15170.
SOURCESearch...

Entry information

Entry nameERF3A_HUMAN
AccessionPrimary (citable) accession number: P15170
Secondary accession number(s): J3KQG6, Q96GF2
Entry history
Integrated into UniProtKB/Swiss-Prot: April 1, 1990
Last sequence update: April 1, 1990
Last modified: July 9, 2014
This is version 141 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program
DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

Relevant documents

SIMILARITY comments

Index of protein domains and families

PDB cross-references

Index of Protein Data Bank (PDB) cross-references

MIM cross-references

Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot

Human chromosome 16

Human chromosome 16: entries, gene names and cross-references to MIM