Reviewed, UniProtKB/Swiss-Prot P15170 (ERF3A_HUMAN)

Names and origin

Protein names

Recommended name:
    Eukaryotic peptide chain release factor GTP-binding subunit ERF3A
      Short name=Eukaryotic peptide chain release factor subunit 3a
      Short name=eRF3a
Alternative name(s):
    G1 to S phase transition protein 1 homolog

Gene names

Name:	GSPT1
Synonyms:	ERF3A

Organism

Homo sapiens (Human)

Taxonomic identifier

9606 [NCBI]

Taxonomic lineage

Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Primates › Haplorrhini › Catarrhini › Hominidae › Homo

Protein attributes

Sequence length	499 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Evidence at protein level.

General annotation (Comments)

Function	Involved in translation termination in response to the termination codons UAA, UAG and UGA. Stimulates the activity of ERF1. Involved in regulation of mammalian cell growth.
Subunit structure	Component of the mRNA surveillance SURF complex, at least composed of ERF1, ERF3 (ERF3A or ERF3B), EEF2, UPF1/RENT1, SMG1, SMG8 and SMG9.
Sequence similarities	Belongs to the GTP-binding elongation factor family. ERF3 subfamily.

Ontologies

Keywords
Biological process	Protein biosynthesis
Ligand	GTP-binding Nucleotide-binding
Gene Ontology (GO)
Biological process	G1/S transition of mitotic cell cycle Ref.1 Traceable author statement. Source: UniProtKB nuclear-transcribed mRNA catabolic process, nonsense-mediated decay Traceable author statement. Source: UniProtKB translation Inferred from electronic annotation. Source: UniProtKB-KW
Cellular component	intracellular Non-traceable author statement. Source: UniProtKB
Molecular function	GTP binding Inferred from electronic annotation. Source: UniProtKB-KW GTPase activity Traceable author statement. Source: UniProtKB protein binding Inferred from physical interaction. Source: UniProtKB translation release factor activity Inferred from mutant phenotype. Source: UniProtKB
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 499	499	Eukaryotic peptide chain release factor GTP-binding subunit ERF3A		PRO_0000091480
Regions
	Nucleotide binding	81 – 88	8	GTP By similarity
	Nucleotide binding	158 – 162	5	GTP By similarity
	Nucleotide binding	220 – 223	4	GTP By similarity

Sequences

	Sequence		Length	Mass (Da)
	P15170-1 [UniParc]. Last modified April 1, 1990. Version 1. Checksum: DE20482CCABC3576	FASTA	499	55,756
	10 20 30 40 50 60 MELSEPIVEN GETEMSPEES WEHKEEISEA EPGGGSLGDG RPPEESAHEM MEEEEEIPKP 70 80 90 100 110 120 KSVVAPPGAP KKEHVNVVFI GHVDAGKSTI GGQIMYLTGM VDKRTLEKYE REAKEKNRET 130 140 150 160 170 180 WYLSWALDTN QEERDKGKTV EVGRAYFETE KKHFTILDAP GHKSFVPNMI GGASQADLAV 190 200 210 220 230 240 LVISARKGEF ETGFEKGGQT REHAMLAKTA GVKHLIVLIN KMDDPTVNWS NERYEECKEK 250 260 270 280 290 300 LVPFLKKVGF NPKKDIHFMP CSGLTGANLK EQSDFCPWYI GLPFIPYLDN LPNFNRSVDG 310 320 330 340 350 360 PIRLPIVDKY KDMGTVVLGK LESGSICKGQ QLVMMPNKHN VEVLGILSDD VETDTVAPGE 370 380 390 400 410 420 NLKIRLKGIE EEEILPGFIL CDPNNLCHSG RTFDAQIVII EHKSIICPGY NAVLHIHTCI 430 440 450 460 470 480 EEVEITALIC LVDKKSGEKS KTRPRFVKQD QVCIARLRTA GTICLETFKD FPQMGRFTLR 490 DEGKTIAIGK VLKLVPEKD

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"A human homologue of the yeast GST1 gene codes for a GTP-binding protein and is expressed in a proliferation-dependent manner in mammalian cells." Hoshino S., Miyazawa H., Enomoto T., Hanaoka F., Kikuchi Y., Kikuchi A., Ui M. EMBO J. 8:3807-3814(1989) [PubMed: 2511002] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA].
[2]	"Genome duplications and other features in 12 Mb of DNA sequence from human chromosome 16p and 16q." Loftus B.J., Kim U.-J., Sneddon V.P., Kalush F., Brandon R., Fuhrmann J., Mason T., Crosby M.L., Barnstead M., Cronin L., Mays A.D., Cao Y., Xu R.X., Kang H.-L., Mitchell S., Eichler E.E., Harris P.C., Venter J.C., Adams M.D. Genomics 60:295-308(1999) [PubMed: 10493829] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
[3]	Colinge J., Superti-Furga G., Bennett K.L. Submitted (OCT-2008) to UniProtKB Cited for: IDENTIFICATION [LARGE SCALE ANALYSIS], MASS SPECTROMETRY.
[4]	"SMG-8 and SMG-9, two novel subunits of the SMG-1 complex, regulate remodeling of the mRNA surveillance complex during nonsense-mediated mRNA decay." Yamashita A., Izumi N., Kashima I., Ohnishi T., Saari B., Katsuhata Y., Muramatsu R., Morita T., Iwamatsu A., Hachiya T., Kurata R., Hirano H., Anderson P., Ohno S. Genes Dev. 23:1091-1105(2009) [PubMed: 19417104] [Abstract] Cited for: IDENTIFICATION IN THE SURF COMPLEX.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBLGenBankDDBJ	X17644 mRNA. Translation: CAA35635.1. U95742 Genomic DNA. Translation: AAB67250.1.
IPI	IPI00218829.
PIR	S06941.
RefSeq	NP_001123479.1.
UniGene	Hs.528780
3D structure databases
HSSP	HSSP built from PDB template 1EFU based on UniProtKB P02990.
ModBase	Search...
Protein-protein interaction databases
IntAct	P15170. 13 interactions.
PTM databases
PhosphoSite	P15170.
Proteomic databases
PRIDE	P15170.
Genome annotation databases
Ensembl	ENSG00000103342. Homo sapiens. [Contig view]
GeneID	2935.
Organism-specific databases
GeneCards	GC16M011874.
H-InvDB	HIX0012824.
HGNC	HGNC:4621. GSPT1.
MIM	139259. gene.
PharmGKB	PA29012.
GenAtlas	Search...
Phylogenomic databases
HOVERGEN	P15170.
Gene expression databases
ArrayExpress	P15170.
Bgee	P15170.
CleanEx	HS_GSPT1.
GermOnline	ENSG00000103342. Homo sapiens.
Family and domain databases
InterPro	IPR000795. ProtSyn_GTP_bd. IPR004161. Transl_elong_EFTu/EF1A_2. IPR004160. Transl_elong_EFTu/EF1A_C. [Graphical view]
Pfam	PF00009. GTP_EFTU. 1 hit. PF03144. GTP_EFTU_D2. 1 hit. PF03143. GTP_EFTU_D3. 1 hit. [Graphical view]
PRINTS	PR00315. ELONGATNFCT.
PROSITE	PS00301. EFACTOR_GTP. 1 hit. [Graphical view]
ProtoNet	Search...
Other Resources
NextBio	11631.
PMAP-CutDB	P15170.
SOURCE	Search...

Entry information

Entry name

ERF3A_HUMAN

Accession

Primary (citable) accession number: P15170

Entry history

Integrated into UniProtKB/Swiss-Prot:	April 1, 1990
Last sequence update:	April 1, 1990
Last modified:	June 16, 2009
This is version 86 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HPI (Human Proteome Initiative)