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P15170

- ERF3A_HUMAN

UniProt

P15170 - ERF3A_HUMAN

Protein

Eukaryotic peptide chain release factor GTP-binding subunit ERF3A

Gene

GSPT1

Organism
Homo sapiens (Human)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at protein leveli
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    • History
      Entry version 143 (01 Oct 2014)
      Sequence version 1 (01 Apr 1990)
      Previous versions | rss
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    Functioni

    Involved in translation termination in response to the termination codons UAA, UAG and UGA. Stimulates the activity of ERF1. Involved in regulation of mammalian cell growth. Component of the transient SURF complex which recruits UPF1 to stalled ribosomes in the context of nonsense-mediated decay (NMD) of mRNAs containing premature stop codons.

    Regions

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Nucleotide bindingi81 – 888GTPBy similarity
    Nucleotide bindingi158 – 1625GTPBy similarity
    Nucleotide bindingi220 – 2234GTPBy similarity

    GO - Molecular functioni

    1. GTPase activity Source: UniProtKB
    2. GTP binding Source: UniProtKB-KW
    3. poly(A) RNA binding Source: UniProtKB
    4. protein binding Source: IntAct
    5. translation release factor activity Source: UniProtKB

    GO - Biological processi

    1. G1/S transition of mitotic cell cycle Source: UniProtKB
    2. GTP catabolic process Source: GOC
    3. nuclear-transcribed mRNA catabolic process, nonsense-mediated decay Source: UniProtKB
    4. protein methylation Source: MGI
    5. translational termination Source: GOC

    Keywords - Biological processi

    Nonsense-mediated mRNA decay, Protein biosynthesis

    Keywords - Ligandi

    GTP-binding, Nucleotide-binding

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Eukaryotic peptide chain release factor GTP-binding subunit ERF3A
    Short name:
    Eukaryotic peptide chain release factor subunit 3a
    Short name:
    eRF3a
    Alternative name(s):
    G1 to S phase transition protein 1 homolog
    Gene namesi
    Name:GSPT1
    Synonyms:ERF3A
    OrganismiHomo sapiens (Human)
    Taxonomic identifieri9606 [NCBI]
    Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo
    ProteomesiUP000005640: Chromosome 16

    Organism-specific databases

    HGNCiHGNC:4621. GSPT1.

    Subcellular locationi

    GO - Cellular componenti

    1. intracellular Source: UniProtKB

    Pathology & Biotechi

    Organism-specific databases

    PharmGKBiPA29012.

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 499499Eukaryotic peptide chain release factor GTP-binding subunit ERF3APRO_0000091480Add
    BLAST

    Proteomic databases

    MaxQBiP15170.
    PaxDbiP15170.
    PRIDEiP15170.

    PTM databases

    PhosphoSiteiP15170.

    Miscellaneous databases

    PMAP-CutDBP15170.

    Expressioni

    Gene expression databases

    ArrayExpressiP15170.
    BgeeiP15170.
    CleanExiHS_GSPT1.
    GenevestigatoriP15170.

    Organism-specific databases

    HPAiHPA052488.

    Interactioni

    Subunit structurei

    Component of the transient SURF (SMG1-UPF1-eRF1-eRF3) complex.1 Publication

    Binary interactionsi

    WithEntry#Exp.IntActNotes
    ETF1P624952EBI-948993,EBI-1047744
    PABPC1P119402EBI-9094806,EBI-81531
    UPF1Q929002EBI-948993,EBI-373471

    Protein-protein interaction databases

    BioGridi109190. 28 interactions.
    IntActiP15170. 8 interactions.
    MINTiMINT-2859376.
    STRINGi9606.ENSP00000398131.

    Structurei

    Secondary structure

    1
    499
    Legend: HelixTurnBeta strand
    Show more details
    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Beta strandi303 – 3053
    Beta strandi308 – 3103
    Beta strandi316 – 3183
    Turni335 – 3373
    Beta strandi340 – 3434
    Beta strandi348 – 3514
    Beta strandi354 – 3563
    Beta strandi364 – 3674
    Beta strandi379 – 3813
    Beta strandi383 – 3853
    Beta strandi392 – 3987
    Beta strandi411 – 4188
    Beta strandi420 – 4289
    Beta strandi452 – 4598
    Beta strandi478 – 4803
    Beta strandi490 – 4945

    3D structure databases

    Select the link destinations:
    PDBe
    RCSB PDB
    PDBj
    Links Updated
    EntryMethodResolution (Å)ChainPositionsPDBsum
    3E1YX-ray3.80E/F/G/H301-499[»]
    3J5Yelectron microscopy9.70B69-496[»]
    3KUIX-ray2.30B64-78[»]
    ProteinModelPortaliP15170.
    SMRiP15170. Positions 2-496.
    ModBaseiSearch...
    MobiDBiSearch...

    Miscellaneous databases

    EvolutionaryTraceiQ96GF2.

    Family & Domainsi

    Domains and Repeats

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Domaini72 – 298227tr-type GPROSITE-ProRule annotationAdd
    BLAST

    Region

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Regioni81 – 888G1PROSITE-ProRule annotation
    Regioni137 – 1415G2PROSITE-ProRule annotation
    Regioni158 – 1614G3PROSITE-ProRule annotation
    Regioni220 – 2234G4PROSITE-ProRule annotation
    Regioni262 – 2643G5PROSITE-ProRule annotation

    Sequence similaritiesi

    Belongs to the TRAFAC class translation factor GTPase superfamily. Classic translation factor GTPase family. ERF3 subfamily.PROSITE-ProRule annotation
    Contains 1 tr-type G (guanine nucleotide-binding) domain.PROSITE-ProRule annotation

    Phylogenomic databases

    eggNOGiCOG5256.
    HOGENOMiHOG000229291.
    HOVERGENiHBG000179.
    InParanoidiP15170.
    KOiK03267.
    OMAiLVVMPNK.
    OrthoDBiEOG76X5ZT.
    PhylomeDBiP15170.
    TreeFamiTF300566.

    Family and domain databases

    Gene3Di3.40.50.300. 1 hit.
    InterProiIPR000795. EF_GTP-bd_dom.
    IPR027417. P-loop_NTPase.
    IPR009000. Transl_B-barrel.
    IPR009001. Transl_elong_EF1A/Init_IF2_C.
    IPR004161. Transl_elong_EFTu/EF1A_2.
    IPR004160. Transl_elong_EFTu/EF1A_C.
    [Graphical view]
    PfamiPF00009. GTP_EFTU. 1 hit.
    PF03144. GTP_EFTU_D2. 1 hit.
    PF03143. GTP_EFTU_D3. 1 hit.
    [Graphical view]
    PRINTSiPR00315. ELONGATNFCT.
    SUPFAMiSSF50447. SSF50447. 1 hit.
    SSF50465. SSF50465. 1 hit.
    SSF52540. SSF52540. 1 hit.
    PROSITEiPS00301. G_TR_1. 1 hit.
    PS51722. G_TR_2. 1 hit.
    [Graphical view]

    Sequences (3)i

    Sequence statusi: Complete.

    This entry describes 3 isoformsi produced by alternative splicing. Align

    Isoform 1 (identifier: P15170-1) [UniParc]FASTAAdd to Basket

    This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

    « Hide

    MELSEPIVEN GETEMSPEES WEHKEEISEA EPGGGSLGDG RPPEESAHEM    50
    MEEEEEIPKP KSVVAPPGAP KKEHVNVVFI GHVDAGKSTI GGQIMYLTGM 100
    VDKRTLEKYE REAKEKNRET WYLSWALDTN QEERDKGKTV EVGRAYFETE 150
    KKHFTILDAP GHKSFVPNMI GGASQADLAV LVISARKGEF ETGFEKGGQT 200
    REHAMLAKTA GVKHLIVLIN KMDDPTVNWS NERYEECKEK LVPFLKKVGF 250
    NPKKDIHFMP CSGLTGANLK EQSDFCPWYI GLPFIPYLDN LPNFNRSVDG 300
    PIRLPIVDKY KDMGTVVLGK LESGSICKGQ QLVMMPNKHN VEVLGILSDD 350
    VETDTVAPGE NLKIRLKGIE EEEILPGFIL CDPNNLCHSG RTFDAQIVII 400
    EHKSIICPGY NAVLHIHTCI EEVEITALIC LVDKKSGEKS KTRPRFVKQD 450
    QVCIARLRTA GTICLETFKD FPQMGRFTLR DEGKTIAIGK VLKLVPEKD 499
    Length:499
    Mass (Da):55,756
    Last modified:April 1, 1990 - v1
    Checksum:iDE20482CCABC3576
    GO
    Isoform 2 (identifier: P15170-2) [UniParc]FASTAAdd to Basket

    The sequence of this isoform differs from the canonical sequence as follows:
         1-1: M → MDPGSGGGGG...CEGSNSAVSM
         8-8: Missing.

    Show »
    Length:636
    Mass (Da):68,601
    Checksum:i31E3FE3DEDF4BE5E
    GO
    Isoform 3 (identifier: P15170-3) [UniParc]FASTAAdd to Basket

    The sequence of this isoform differs from the canonical sequence as follows:
         1-1: M → MDPGSGGGGG...CEGSNSAVSM

    Note: Gene prediction based on EST data.

    Show »
    Length:637
    Mass (Da):68,700
    Checksum:i7765D73A284F35C4
    GO

    Experimental Info

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Isoform 2 (identifier: P15170-2)
    Sequence conflicti6 – 94GGGG → G in AAH09503. (PubMed:15489334)Curated
    Sequence conflicti92 – 921G → C in AAH09503. (PubMed:15489334)Curated
    Sequence conflicti100 – 1001V → A in AAH09503. (PubMed:15489334)Curated

    Alternative sequence

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Alternative sequencei1 – 11M → MDPGSGGGGGGGGGGGSSSG SSSSDSAPDCWDQADMEAPG PGPCGGGGSLAAAAEAQREN LSAAFSRQLNVNAKPFVPNV HAAEFVPSFLRGPAAPPPPV GGAANNHGAGSGAGGRAAPV ESSQEEQSLCEGSNSAVSM in isoform 2 and isoform 3. 1 PublicationVSP_042198
    Alternative sequencei8 – 81Missing in isoform 2. 1 PublicationVSP_042199

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    X17644 mRNA. Translation: CAA35635.1.
    U95742 Genomic DNA. Translation: AAB67250.1.
    AC007216 Genomic DNA. No translation available.
    BC009503 mRNA. Translation: AAH09503.2.
    CCDSiCCDS45412.1. [P15170-3]
    CCDS45413.1. [P15170-2]
    CCDS45414.1. [P15170-1]
    PIRiS06941.
    RefSeqiNP_001123478.1. NM_001130006.1.
    NP_001123479.1. NM_001130007.1. [P15170-1]
    UniGeneiHs.528780.

    Genome annotation databases

    EnsembliENST00000420576; ENSP00000399539; ENSG00000103342. [P15170-1]
    ENST00000434724; ENSP00000398131; ENSG00000103342. [P15170-3]
    ENST00000439887; ENSP00000408399; ENSG00000103342. [P15170-2]
    ENST00000563468; ENSP00000454351; ENSG00000103342. [P15170-1]
    GeneIDi2935.
    KEGGihsa:2935.
    UCSCiuc002dbu.3. human. [P15170-2]
    uc010bux.3. human. [P15170-1]

    Polymorphism databases

    DMDMi121688.

    Keywords - Coding sequence diversityi

    Alternative splicing

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    X17644 mRNA. Translation: CAA35635.1 .
    U95742 Genomic DNA. Translation: AAB67250.1 .
    AC007216 Genomic DNA. No translation available.
    BC009503 mRNA. Translation: AAH09503.2 .
    CCDSi CCDS45412.1. [P15170-3 ]
    CCDS45413.1. [P15170-2 ]
    CCDS45414.1. [P15170-1 ]
    PIRi S06941.
    RefSeqi NP_001123478.1. NM_001130006.1.
    NP_001123479.1. NM_001130007.1. [P15170-1 ]
    UniGenei Hs.528780.

    3D structure databases

    Select the link destinations:
    PDBe
    RCSB PDB
    PDBj
    Links Updated
    Entry Method Resolution (Å) Chain Positions PDBsum
    3E1Y X-ray 3.80 E/F/G/H 301-499 [» ]
    3J5Y electron microscopy 9.70 B 69-496 [» ]
    3KUI X-ray 2.30 B 64-78 [» ]
    ProteinModelPortali P15170.
    SMRi P15170. Positions 2-496.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    BioGridi 109190. 28 interactions.
    IntActi P15170. 8 interactions.
    MINTi MINT-2859376.
    STRINGi 9606.ENSP00000398131.

    PTM databases

    PhosphoSitei P15170.

    Polymorphism databases

    DMDMi 121688.

    Proteomic databases

    MaxQBi P15170.
    PaxDbi P15170.
    PRIDEi P15170.

    Protocols and materials databases

    DNASUi 2935.
    Structural Biology Knowledgebase Search...

    Genome annotation databases

    Ensembli ENST00000420576 ; ENSP00000399539 ; ENSG00000103342 . [P15170-1 ]
    ENST00000434724 ; ENSP00000398131 ; ENSG00000103342 . [P15170-3 ]
    ENST00000439887 ; ENSP00000408399 ; ENSG00000103342 . [P15170-2 ]
    ENST00000563468 ; ENSP00000454351 ; ENSG00000103342 . [P15170-1 ]
    GeneIDi 2935.
    KEGGi hsa:2935.
    UCSCi uc002dbu.3. human. [P15170-2 ]
    uc010bux.3. human. [P15170-1 ]

    Organism-specific databases

    CTDi 2935.
    GeneCardsi GC16M011961.
    HGNCi HGNC:4621. GSPT1.
    HPAi HPA052488.
    MIMi 139259. gene.
    neXtProti NX_P15170.
    PharmGKBi PA29012.
    GenAtlasi Search...

    Phylogenomic databases

    eggNOGi COG5256.
    HOGENOMi HOG000229291.
    HOVERGENi HBG000179.
    InParanoidi P15170.
    KOi K03267.
    OMAi LVVMPNK.
    OrthoDBi EOG76X5ZT.
    PhylomeDBi P15170.
    TreeFami TF300566.

    Miscellaneous databases

    ChiTaRSi GSPT1. human.
    EvolutionaryTracei Q96GF2.
    GeneWikii GSPT1.
    GenomeRNAii 2935.
    NextBioi 11631.
    PMAP-CutDB P15170.
    PROi P15170.
    SOURCEi Search...

    Gene expression databases

    ArrayExpressi P15170.
    Bgeei P15170.
    CleanExi HS_GSPT1.
    Genevestigatori P15170.

    Family and domain databases

    Gene3Di 3.40.50.300. 1 hit.
    InterProi IPR000795. EF_GTP-bd_dom.
    IPR027417. P-loop_NTPase.
    IPR009000. Transl_B-barrel.
    IPR009001. Transl_elong_EF1A/Init_IF2_C.
    IPR004161. Transl_elong_EFTu/EF1A_2.
    IPR004160. Transl_elong_EFTu/EF1A_C.
    [Graphical view ]
    Pfami PF00009. GTP_EFTU. 1 hit.
    PF03144. GTP_EFTU_D2. 1 hit.
    PF03143. GTP_EFTU_D3. 1 hit.
    [Graphical view ]
    PRINTSi PR00315. ELONGATNFCT.
    SUPFAMi SSF50447. SSF50447. 1 hit.
    SSF50465. SSF50465. 1 hit.
    SSF52540. SSF52540. 1 hit.
    PROSITEi PS00301. G_TR_1. 1 hit.
    PS51722. G_TR_2. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "A human homologue of the yeast GST1 gene codes for a GTP-binding protein and is expressed in a proliferation-dependent manner in mammalian cells."
      Hoshino S., Miyazawa H., Enomoto T., Hanaoka F., Kikuchi Y., Kikuchi A., Ui M.
      EMBO J. 8:3807-3814(1989) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 1).
    2. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    3. "The sequence and analysis of duplication-rich human chromosome 16."
      Martin J., Han C., Gordon L.A., Terry A., Prabhakar S., She X., Xie G., Hellsten U., Chan Y.M., Altherr M., Couronne O., Aerts A., Bajorek E., Black S., Blumer H., Branscomb E., Brown N.C., Bruno W.J.
      , Buckingham J.M., Callen D.F., Campbell C.S., Campbell M.L., Campbell E.W., Caoile C., Challacombe J.F., Chasteen L.A., Chertkov O., Chi H.C., Christensen M., Clark L.M., Cohn J.D., Denys M., Detter J.C., Dickson M., Dimitrijevic-Bussod M., Escobar J., Fawcett J.J., Flowers D., Fotopulos D., Glavina T., Gomez M., Gonzales E., Goodstein D., Goodwin L.A., Grady D.L., Grigoriev I., Groza M., Hammon N., Hawkins T., Haydu L., Hildebrand C.E., Huang W., Israni S., Jett J., Jewett P.B., Kadner K., Kimball H., Kobayashi A., Krawczyk M.-C., Leyba T., Longmire J.L., Lopez F., Lou Y., Lowry S., Ludeman T., Manohar C.F., Mark G.A., McMurray K.L., Meincke L.J., Morgan J., Moyzis R.K., Mundt M.O., Munk A.C., Nandkeshwar R.D., Pitluck S., Pollard M., Predki P., Parson-Quintana B., Ramirez L., Rash S., Retterer J., Ricke D.O., Robinson D.L., Rodriguez A., Salamov A., Saunders E.H., Scott D., Shough T., Stallings R.L., Stalvey M., Sutherland R.D., Tapia R., Tesmer J.G., Thayer N., Thompson L.S., Tice H., Torney D.C., Tran-Gyamfi M., Tsai M., Ulanovsky L.E., Ustaszewska A., Vo N., White P.S., Williams A.L., Wills P.L., Wu J.-R., Wu K., Yang J., DeJong P., Bruce D., Doggett N.A., Deaven L., Schmutz J., Grimwood J., Richardson P., Rokhsar D.S., Eichler E.E., Gilna P., Lucas S.M., Myers R.M., Rubin E.M., Pennacchio L.A.
      Nature 432:988-994(2004) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    4. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
      The MGC Project Team
      Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 2).
      Tissue: Lung.
    5. "SMG-8 and SMG-9, two novel subunits of the SMG-1 complex, regulate remodeling of the mRNA surveillance complex during nonsense-mediated mRNA decay."
      Yamashita A., Izumi N., Kashima I., Ohnishi T., Saari B., Katsuhata Y., Muramatsu R., Morita T., Iwamatsu A., Hachiya T., Kurata R., Hirano H., Anderson P., Ohno S.
      Genes Dev. 23:1091-1105(2009) [PubMed] [Europe PMC] [Abstract]
      Cited for: IDENTIFICATION IN THE SURF COMPLEX.
    6. Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
    7. "Molecular basis of eRF3 recognition by the MLLE domain of poly(A)-binding protein."
      Kozlov G., Gehring K.
      PLoS ONE 5:E10169-E10169(2010) [PubMed] [Europe PMC] [Abstract]
      Cited for: X-RAY CRYSTALLOGRAPHY (1.40 ANGSTROMS) OF 73-87 (ISOFORM 2).

    Entry informationi

    Entry nameiERF3A_HUMAN
    AccessioniPrimary (citable) accession number: P15170
    Secondary accession number(s): J3KQG6, Q96GF2
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: April 1, 1990
    Last sequence update: April 1, 1990
    Last modified: October 1, 2014
    This is version 143 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programChordata Protein Annotation Program
    DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

    Miscellaneousi

    Keywords - Technical termi

    3D-structure, Complete proteome, Reference proteome

    Documents

    1. Human chromosome 16
      Human chromosome 16: entries, gene names and cross-references to MIM
    2. MIM cross-references
      Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot
    3. PDB cross-references
      Index of Protein Data Bank (PDB) cross-references
    4. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3