Reviewed, UniProtKB/Swiss-Prot P15157 (TRYA1_HUMAN)

Names and origin

Protein names

Recommended name:
    Tryptase alpha-1
      Short name=Tryptase-1
    EC=3.4.21.59

Gene names

Name:	TPSAB1
Synonyms:	TPS1, TPS2, TPSB1

Organism

Homo sapiens (Human)

Taxonomic identifier

9606 [NCBI]

Taxonomic lineage

Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Primates › Haplorrhini › Catarrhini › Hominidae › Homo

Protein attributes

Sequence length	275 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Evidence at protein level.

General annotation (Comments)

Function	Tryptase is the major neutral protease present in mast cells and is secreted upon the coupled activation-degranulation response of this cell type.
Catalytic activity	Preferential cleavage: Arg-|-Xaa, Lys-|-Xaa, but with more restricted specificity than trypsin.
Subunit structure	Homotetramer.
Subcellular location	Secreted. Note: Released from the secretory granules upon mast cell activation.
Sequence similarities	Belongs to the peptidase S1 family. Tryptase subfamily. Contains 1 peptidase S1 domain.

Ontologies

Keywords
Cellular component	Secreted
Coding sequence diversity	Alternative splicing Polymorphism
Domain	Signal
Molecular function	Hydrolase Protease Serine protease
PTM	Disulfide bond Glycoprotein Zymogen
Technical term	3D-structure Direct protein sequencing
Gene Ontology (GO)
Biological process	defense response Ref.1 Traceable author statement. Source: ProtInc proteolysis Inferred from electronic annotation. Source: InterPro
Cellular component	extracellular region Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	protein binding Inferred from physical interaction. Source: IntAct serine-type endopeptidase activity Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Binary interactions

With	Entry	#Exp.	IntAct	Notes
NCK1	P16333	1	EBI-1762849,EBI-389883
PIK3R1	P27986	1	EBI-1762849,EBI-79464

Alternative products

This entry describes 2 isoforms produced by alternative splicing. [Align] [Select]

Isoform 1 (identifier: P15157-1)

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

Isoform 2 (identifier: P15157-2)

The sequence of this isoform differs from the canonical sequence as follows:
     79-87: Missing.

Sequence annotation (Features)

Feature key

Position(s)

Length

Description

Graphical view

Feature identifier

Molecule processing

Signal peptide

1 – 18

18

Potential

Propeptide

19 – 30

12

Activation peptide Ref.5

PRO_0000027477

Chain

31 – 275

245

Tryptase alpha-1

PRO_0000027478

Regions

Domain

31 – 272

242

Peptidase S1

Sites

Active site

74

1

Charge relay system

Active site

121

1

Charge relay system

Active site

224

1

Charge relay system

Amino acid modifications

Glycosylation

132

1

N-linked (GlcNAc...) Potential

Glycosylation

233

1

N-linked (GlcNAc...) Potential

Disulfide bond

59 ↔ 75

Disulfide bond

155 ↔ 230

Disulfide bond

188 ↔ 211

Disulfide bond

220 ↔ 248

Natural variations

Alternative sequence

79 – 87

9

Missing in isoform 2.

VSP_005374

Natural variant

15

1

R → P in alpha-II.

VAR_012102

Natural variant

221

1

K → Q in alpha-II. dbSNP rs1137382.

VAR_012103

Experimental info

Sequence conflict

215 – 216

2

TR → SQ in AAA86934. Ref.1

Secondary structure

1

.

.

.

.

.

.

.

.

.

.

.

.

.

.

.

.

.

.

.

.

.

.

.

.

.

.

.

.

.

.

.

.

.

.

.

.

.

.

.

.

.

.

275

Helix Strand Turn

Details...

Beta strand

45 – 65

21

Beta strand

68 – 71

4

Helix

73 – 76

4

Helix

83 – 85

3

Beta strand

86 – 90

5

Turn

95 – 98

4

Beta strand

104 – 109

6

Helix

116 – 118

3

Beta strand

123 – 129

7

Beta strand

135 – 137

3

Beta strand

154 – 160

7

Beta strand

176 – 182

7

Helix

185 – 193

9

Beta strand

196 – 198

3

Beta strand

209 – 212

4

Beta strand

215 – 218

4

Beta strand

227 – 232

6

Beta strand

235 – 244

10

Beta strand

248 – 250

3

Beta strand

255 – 259

5

Helix

260 – 263

4

Helix

264 – 270

7

Sequences

	Sequence		Length	Mass (Da)
	Isoform 1 [UniParc]. Last modified October 18, 2001. Version 3. Checksum: B9BAC4BBCB91CE75	FASTA	275	30,773
	10 20 30 40 50 60 MLSLLLLALP VLASRAYAAP APVQALQQAG IVGGQEAPRS KWPWQVSLRV RDRYWMHFCG 70 80 90 100 110 120 GSLIHPQWVL TAAHCLGPDV KDLATLRVQL REQHLYYQDQ LLPVSRIIVH PQFYIIQTGA 130 140 150 160 170 180 DIALLELEEP VNISSRVHTV MLPPASETFP PGMPCWVTGW GDVDNDEPLP PPFPLKQVKV 190 200 210 220 230 240 PIMENHICDA KYHLGAYTGD DVRIIRDDML CAGNTRRDSC KGDSGGPLVC KVNGTWLQAG 250 260 270 VVSWDEGCAQ PNRPGIYTRV TYYLDWIHHY VPKKP
	Isoform 2. Checksum: C3812E3A77A31257	FASTA	266	29,760
	10 20 30 40 50 60 MLSLLLLALP VLASRAYAAP APVQALQQAG IVGGQEAPRS KWPWQVSLRV RDRYWMHFCG 70 80 90 100 110 120 GSLIHPQWVL TAAHCLGPVQ LREQHLYYQD QLLPVSRIIV HPQFYIIQTG ADIALLELEE 130 140 150 160 170 180 PVNISSRVHT VMLPPASETF PPGMPCWVTG WGDVDNDEPL PPPFPLKQVK VPIMENHICD 190 200 210 220 230 240 AKYHLGAYTG DDVRIIRDDM LCAGNTRRDS CKGDSGGPLV CKVNGTWLQA GVVSWDEGCA 250 260 QPNRPGIYTR VTYYLDWIHH YVPKKP

References

[1]	"Cloning and characterization of complementary DNA for human tryptase." Miller J.S., Westin E.H., Schwartz L.B. J. Clin. Invest. 84:1188-1195(1989) [PubMed: 2677049] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 1). Tissue: Lung.
[2]	Schwartz L.B. Submitted (MAR-1990) to the EMBL/GenBank/DDBJ databases Cited for: SEQUENCE REVISION TO 89-93 AND 108.
[3]	"Characterization of genes encoding known and novel human mast cell tryptases on chromosome 16p13.3." Pallaoro M., Fejzo M.S., Shayesteh L., Blount J.L., Caughey G.H. J. Biol. Chem. 274:3355-3362(1999) [PubMed: 9920877] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
[4]	"Molecular cloning and characterization of novel human tryptase cDNAs and splicing variants." Wang H.W., McNeil H.P., Thomas P.S., Murphy B.N., Webster M.J., Hettiaratchi A., King G., Heywood G.J., Huang C., Stevens R.L., Hunt J.E. Submitted (NOV-1999) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORMS 1 AND 2). Tissue: Lung.
[5]	"Human pituitary tryptase: molecular forms, NH2-terminal sequence, immunocytochemical localization, and specificity with prohormone and fluorogenic substrates." Cromlish J.A., Seidah N.G., Marcinkiewcz M., Hamelin J., Johnson D.A., Chretein M. J. Biol. Chem. 262:1363-1373(1987) [PubMed: 3543004] [Abstract] Cited for: PROTEIN SEQUENCE OF 31-50, PROTEIN SEQUENCE OF 31-38. Tissue: Lung.
+	Additional computationally mapped references.

Cross-references

Sequence databases

EMBLGenBankDDBJ

M30038 mRNA. Translation: AAA86934.1.
AF098328 Genomic DNA. Translation: AAD17846.1.
AF206665 mRNA. Translation: AAG35695.1.
AF206666 mRNA. Translation: AAG35696.1.

IPI

IPI00010274.
IPI00219931.

PIR

A45754.

UniGene

Hs.405479

3D structure databases

PDBPDBe

Entry	Method	Resolution (Å)	Chain	Positions	PDBsum
1LTO	X-ray	2.20	A/B/C/D	31-275	[»]
2F9N	X-ray	1.60	A/B/C/D	31-275	[»]
2F9O	X-ray	2.10	A/B/C/D	31-275	[»]
2F9P	X-ray	2.30	A/B/C/D	31-275	[»]

ModBase

Search...

Protein-protein interaction databases

IntAct

P15157. 2 interactions.

Protein family/group databases

MEROPS

S01.143.

PTM databases

PhosphoSite

P15157.

Proteomic databases

PRIDE

P15157.

Genome annotation databases

Ensembl

ENSG00000197253. Homo sapiens. [Contig view]

Organism-specific databases

GeneCards

GC16P001233.

HGNC

HGNC:12019. TPSAB1.

MIM

191080. gene.

PharmGKB

PA36698.

GenAtlas

Search...

Phylogenomic databases

HOVERGEN

P15157.

Enzyme and pathway databases

BRENDA

3.4.21.59. 247.

Gene expression databases

CleanEx

HS_TPSAB1.

GermOnline

ENSG00000197253. Homo sapiens.

Family and domain databases

InterPro

IPR018114. Peptidase_S1/S6_AS.
IPR001254. Peptidase_S1_S6.
IPR001314. Peptidase_S1A.
[Graphical view]

Pfam

PF00089. Trypsin. 1 hit.
[Graphical view]

PRINTS

PR00722. CHYMOTRYPSIN.

SMART

SM00020. Tryp_SPc. 1 hit.
[Graphical view]

PROSITE

PS50240. TRYPSIN_DOM. 1 hit.
PS00134. TRYPSIN_HIS. 1 hit.
PS00135. TRYPSIN_SER. 1 hit.
[Graphical view]

ProtoNet

Search...

Other Resources

SOURCE

Search...

Entry information

Entry name

TRYA1_HUMAN

Accession

Primary (citable) accession number: P15157
Secondary accession number(s): Q9H2Y5, Q9UQI1

Entry history

Integrated into UniProtKB/Swiss-Prot:	April 1, 1990
Last sequence update:	October 18, 2001
Last modified:	June 16, 2009
This is version 103 of the entry and version 3 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HPI (Human Proteome Initiative)