Skip Header

You are using a version of Internet Explorer that may not display all features of this website. Please upgrade to a modern browser.
Contribute Send feedback
Read comments (?) or add your own

P15155 (VP4_ROTGI) Reviewed, UniProtKB/Swiss-Prot

Last modified February 19, 2014. Version 67. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (1) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Outer capsid protein VP4
Alternative name(s):
Hemagglutinin

Cleaved into the following 2 chains:

  1. Outer capsid protein VP8*
  2. Outer capsid protein VP5*
OrganismRotavirus B (isolate Rat/United States/IDIR/1984) (RV-B) (Rotavirus B (isolate infectious diarrhea of infant rats))
Taxonomic identifier28877 [NCBI]
Taxonomic lineageVirusesdsRNA virusesReoviridaeSedoreovirinaeRotavirus
Virus hostHomo sapiens (Human) [TaxID: 9606]
Rattus norvegicus (Rat) [TaxID: 10116]

Protein attributes

Sequence length751 AA.
Sequence statusComplete.
Sequence processingThe displayed sequence is further processed into a mature form.
Protein existenceInferred from homology

General annotation (Comments)

Function

Spike-forming protein that mediates virion attachment to the host epithelial cell receptors and plays a major role in cell penetration, determination of host range restriction and virulence. Rotavirus entry into the host cell probably involves multiple sequential contacts between the outer capsid proteins VP4 and VP7, and the cell receptors By similarity.

Outer capsid protein VP5*: forms the spike "foot" and "body". Acts as a membrane permeabilization protein that mediates release of viral particles from endosomal compartments into the cytoplasm By similarity.

VP8* forms the head of the spikes By similarity.

Subunit structure

VP4 is a homotrimer Potential.

Subcellular location

Outer capsid protein VP4: Virion By similarity. Host rough endoplasmic reticulum Potential. Note: Immature double-layered particles assembled in the cytoplasm bud across the membrane of the endoplasmic reticulum, acquiring during this process a transient lipid membrane that is modified with the ER resident viral glycoproteins NSP4 and VP7; these enveloped particles also contain VP4. As the particles move towards the interior of the ER cisternae, the transient lipid membrane and the non-structural protein NSP4 are lost, while the virus surface proteins VP4 and VP7 rearrange to form the outermost virus protein layer, yielding mature infectious triple-layered particles By similarity.

Outer capsid protein VP8*: Virion By similarity. Note: Outer capsid protein By similarity.

Outer capsid protein VP5*: Virion By similarity. Note: Outer capsid protein By similarity.

Post-translational modification

Proteolytic cleavage by trypsin results in activation of VP4 functions and greatly increases infectivity. The penetration into the host cell is dependent on trypsin treatment of VP4. It produces two peptides, VP5* and VP8* that remain associated with the virion By similarity.

Sequence similarities

Belongs to the rotavirus VP4 family.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 751751Outer capsid protein VP4
PRO_0000149539
Chain1 – 207207Outer capsid protein VP8* Potential
PRO_0000369840
Chain215 – 751537Outer capsid protein VP5* Potential
PRO_0000369841

Sites

Site207 – 2082Cleavage Potential
Site214 – 2152Cleavage Potential

Amino acid modifications

Glycosylation231N-linked (GlcNAc...); by host Potential
Glycosylation1411N-linked (GlcNAc...); by host Potential
Glycosylation2111N-linked (GlcNAc...); by host Potential
Glycosylation2761N-linked (GlcNAc...); by host Potential
Glycosylation2921N-linked (GlcNAc...); by host Potential
Glycosylation5281N-linked (GlcNAc...); by host Potential
Glycosylation6781N-linked (GlcNAc...); by host Potential
Glycosylation6991N-linked (GlcNAc...); by host Potential

Sequences

Sequence LengthMass (Da)Tools
P15155 [UniParc].

Last modified April 1, 1990. Version 1.
Checksum: 756A33227C065709

FASTA75185,558
        10         20         30         40         50         60 
MLAYLRREWQ SYGETVIAEG TFNSTSSDSS QSEKPIKTDG RYCYQAEIGR NAYSMDARGF 

        70         80         90        100        110        120 
MLGESDRHVD TTQFLPYTGY ITDGIKYCNI EPPCGTLLRM HFDVSGDVSV DMHRVASIYV 

       130        140        150        160        170        180 
EVDSVTYDGS QYTIRGYRDR NLTATETQKK LIFYGFRKLG MIGMTGNGRV MLTSTIIQKI 

       190        200        210        220        230        240 
SYKHQFTFQM HSESYVWGPC SGRIKTRVQG NDSRIIIYEQ EDGFWKILKE TLWIKLKPYF 

       250        260        270        280        290        300 
KPYGTMGGAF KNWLIDSGFE KHEYTYSYER DGQVVNATTV TYVKPTGKAG INQSWRPATD 

       310        320        330        340        350        360 
YNGQFTVLQP EDEFSVWYFE DKWQISQAIY AKNFQSDSQV EGELTNNGAL IFKMNYIPSL 

       370        380        390        400        410        420 
AGITNKGGKV KYRYISGGFA QIDTSRHTGL AIILNFKCYG KKFYADNNNY PVDNALNPYI 

       430        440        450        460        470        480 
CYIGDSYTLS GGTHYRQGAC AGFAAGYDDE ITEHDMTISY TVMKPSDPDF VTGGDNYGQT 

       490        500        510        520        530        540 
VTSDIERSIR DLQDQINSIL AEMNIQQVTS AVFTAITNLG ELPSLFSNIT KVFNRAKDNI 

       550        560        570        580        590        600 
KKLRSRSNSD ISPIGATKII DKTTLETPQL SVINRMPEEY ELGIIYNSMR TRKLIDERKH 

       610        620        630        640        650        660 
DFDTFAVATE MELPYISKVN TLTKEFKDYL KKPGLLSNDD VAVQIDPMNN RLSVLRRKYA 

       670        680        690        700        710        720 
DIIEYKIDPE LAHEVLSNMS NSATRSLFSL NVRKQIAMNN SFSEPTFSQI IDRMFDDGQL 

       730        740        750 
IDVLNNLNRE TATELFDEFL TRIKSMLVKM S 

« Hide

References

[1]"The complete nucleic acid sequence of gene segment 3 of the IDIR strain of group B rotavirus."
Sato S., Yolken R.H., Eiden J.J.
Nucleic Acids Res. 17:10113-10113(1989) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC RNA].

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
X16949 Genomic RNA. Translation: CAA34823.1.
PIRWMXRGB. A33093.

3D structure databases

ProteinModelPortalP15155.
ModBaseSearch...
MobiDBSearch...

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Family and domain databases

ProtoNetSearch...

Entry information

Entry nameVP4_ROTGI
AccessionPrimary (citable) accession number: P15155
Entry history
Integrated into UniProtKB/Swiss-Prot: April 1, 1990
Last sequence update: April 1, 1990
Last modified: February 19, 2014
This is version 67 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programViral Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families