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P15155

- VP4_ROTGI

UniProt

P15155 - VP4_ROTGI

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Protein

Outer capsid protein VP4

Gene
N/A
Organism
Rotavirus B (isolate Rat/United States/IDIR/1984) (RV-B) (Rotavirus B (isolate infectious diarrhea of infant rats))
Status
Reviewed - Annotation score: 3 out of 5 - Protein inferred from homologyi

Functioni

Spike-forming protein that mediates virion attachment to the host epithelial cell receptors and plays a major role in cell penetration, determination of host range restriction and virulence. Rotavirus entry into the host cell probably involves multiple sequential contacts between the outer capsid proteins VP4 and VP7, and the cell receptors By similarity.
Outer capsid protein VP5*: forms the spike "foot" and "body". Acts as a membrane permeabilization protein that mediates release of viral particles from endosomal compartments into the cytoplasm By similarity.
VP8* forms the head of the spikes By similarity.

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sitei207 – 2082Cleavage Reviewed prediction
Sitei214 – 2152Cleavage Reviewed prediction

GO - Biological processi

  1. permeabilization of host organelle membrane involved in viral entry into host cell Source: UniProtKB-KW
  2. virion attachment to host cell Source: UniProtKB-KW
Complete GO annotation...

Keywords - Molecular functioni

Hemagglutinin

Keywords - Biological processi

Host-virus interaction, Viral attachment to host cell, Viral penetration into host cytoplasm, Viral penetration via permeabilization of host membrane, Virus entry into host cell

Names & Taxonomyi

Protein namesi
Recommended name:
Outer capsid protein VP4
Alternative name(s):
Hemagglutinin
Cleaved into the following 2 chains:
OrganismiRotavirus B (isolate Rat/United States/IDIR/1984) (RV-B) (Rotavirus B (isolate infectious diarrhea of infant rats))
Taxonomic identifieri28877 [NCBI]
Taxonomic lineageiVirusesdsRNA virusesReoviridaeSedoreovirinaeRotavirus
Virus hostiHomo sapiens (Human) [TaxID: 9606]
Rattus norvegicus (Rat) [TaxID: 10116]

Subcellular locationi

Chain Outer capsid protein VP4 : Virion By similarity. Host rough endoplasmic reticulum Reviewed prediction
Note: Immature double-layered particles assembled in the cytoplasm bud across the membrane of the endoplasmic reticulum, acquiring during this process a transient lipid membrane that is modified with the ER resident viral glycoproteins NSP4 and VP7; these enveloped particles also contain VP4. As the particles move towards the interior of the ER cisternae, the transient lipid membrane and the non-structural protein NSP4 are lost, while the virus surface proteins VP4 and VP7 rearrange to form the outermost virus protein layer, yielding mature infectious triple-layered particles By similarity.
Chain Outer capsid protein VP8* : Virion By similarity
Note: Outer capsid protein By similarity.
Chain Outer capsid protein VP5* : Virion By similarity
Note: Outer capsid protein By similarity.

GO - Cellular componenti

  1. host cell rough endoplasmic reticulum Source: UniProtKB-SubCell
  2. viral outer capsid Source: UniProtKB-KW
Complete GO annotation...

Keywords - Cellular componenti

Capsid protein, Host endoplasmic reticulum, Outer capsid protein, Virion

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 751751Outer capsid protein VP4PRO_0000149539Add
BLAST
Chaini1 – 207207Outer capsid protein VP8* Reviewed predictionPRO_0000369840Add
BLAST
Chaini215 – 751537Outer capsid protein VP5* Reviewed predictionPRO_0000369841Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Glycosylationi23 – 231N-linked (GlcNAc...); by host Reviewed prediction
Glycosylationi141 – 1411N-linked (GlcNAc...); by host Reviewed prediction
Glycosylationi211 – 2111N-linked (GlcNAc...); by host Reviewed prediction
Glycosylationi276 – 2761N-linked (GlcNAc...); by host Reviewed prediction
Glycosylationi292 – 2921N-linked (GlcNAc...); by host Reviewed prediction
Glycosylationi528 – 5281N-linked (GlcNAc...); by host Reviewed prediction
Glycosylationi678 – 6781N-linked (GlcNAc...); by host Reviewed prediction
Glycosylationi699 – 6991N-linked (GlcNAc...); by host Reviewed prediction

Post-translational modificationi

Proteolytic cleavage by trypsin results in activation of VP4 functions and greatly increases infectivity. The penetration into the host cell is dependent on trypsin treatment of VP4. It produces two peptides, VP5* and VP8* that remain associated with the virion By similarity.

Keywords - PTMi

Glycoprotein

Interactioni

Subunit structurei

VP4 is a homotrimer Reviewed prediction.

Structurei

3D structure databases

ProteinModelPortaliP15155.

Family & Domainsi

Sequence similaritiesi

Belongs to the rotavirus VP4 family.

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

P15155-1 [UniParc]FASTAAdd to Basket

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MLAYLRREWQ SYGETVIAEG TFNSTSSDSS QSEKPIKTDG RYCYQAEIGR    50
NAYSMDARGF MLGESDRHVD TTQFLPYTGY ITDGIKYCNI EPPCGTLLRM 100
HFDVSGDVSV DMHRVASIYV EVDSVTYDGS QYTIRGYRDR NLTATETQKK 150
LIFYGFRKLG MIGMTGNGRV MLTSTIIQKI SYKHQFTFQM HSESYVWGPC 200
SGRIKTRVQG NDSRIIIYEQ EDGFWKILKE TLWIKLKPYF KPYGTMGGAF 250
KNWLIDSGFE KHEYTYSYER DGQVVNATTV TYVKPTGKAG INQSWRPATD 300
YNGQFTVLQP EDEFSVWYFE DKWQISQAIY AKNFQSDSQV EGELTNNGAL 350
IFKMNYIPSL AGITNKGGKV KYRYISGGFA QIDTSRHTGL AIILNFKCYG 400
KKFYADNNNY PVDNALNPYI CYIGDSYTLS GGTHYRQGAC AGFAAGYDDE 450
ITEHDMTISY TVMKPSDPDF VTGGDNYGQT VTSDIERSIR DLQDQINSIL 500
AEMNIQQVTS AVFTAITNLG ELPSLFSNIT KVFNRAKDNI KKLRSRSNSD 550
ISPIGATKII DKTTLETPQL SVINRMPEEY ELGIIYNSMR TRKLIDERKH 600
DFDTFAVATE MELPYISKVN TLTKEFKDYL KKPGLLSNDD VAVQIDPMNN 650
RLSVLRRKYA DIIEYKIDPE LAHEVLSNMS NSATRSLFSL NVRKQIAMNN 700
SFSEPTFSQI IDRMFDDGQL IDVLNNLNRE TATELFDEFL TRIKSMLVKM 750
S 751
Length:751
Mass (Da):85,558
Last modified:April 1, 1990 - v1
Checksum:i756A33227C065709
GO

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
X16949 Genomic RNA. Translation: CAA34823.1.
PIRiA33093. WMXRGB.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
X16949 Genomic RNA. Translation: CAA34823.1 .
PIRi A33093. WMXRGB.

3D structure databases

ProteinModelPortali P15155.
ModBasei Search...
MobiDBi Search...

Protocols and materials databases

Structural Biology Knowledgebase Search...

Family and domain databases

ProtoNeti Search...

Publicationsi

  1. "The complete nucleic acid sequence of gene segment 3 of the IDIR strain of group B rotavirus."
    Sato S., Yolken R.H., Eiden J.J.
    Nucleic Acids Res. 17:10113-10113(1989) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC RNA].

Entry informationi

Entry nameiVP4_ROTGI
AccessioniPrimary (citable) accession number: P15155
Entry historyi
Integrated into UniProtKB/Swiss-Prot: April 1, 1990
Last sequence update: April 1, 1990
Last modified: February 19, 2014
This is version 67 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programViral Protein Annotation Program

Miscellaneousi

Documents

  1. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

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