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P15150 (C11B1_BOVIN) Reviewed, UniProtKB/Swiss-Prot

Last modified January 22, 2014. Version 113. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (1) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Cytochrome P450 11B1, mitochondrial
Alternative name(s):
CYPXIB1
Cytochrome P450C11
Steroid 11-beta-hydroxylase
EC=1.14.15.4
Gene names
Name:CYP11B1
OrganismBos taurus (Bovine) [Reference proteome]
Taxonomic identifier9913 [NCBI]
Taxonomic lineageEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaLaurasiatheriaCetartiodactylaRuminantiaPecoraBovidaeBovinaeBos

Protein attributes

Sequence length503 AA.
Sequence statusComplete.
Sequence processingThe displayed sequence is further processed into a mature form.
Protein existenceEvidence at protein level

General annotation (Comments)

Function

Has steroid 11-beta-hydroxylase activity. In addition to this activity, the 18 or 19-hydroxylation of steroids and the aromatization of androstendione to estrone have also been ascribed to cytochrome P450 XIB.

Catalytic activity

A steroid + reduced adrenodoxin + O2 = an 11-beta-hydroxysteroid + oxidized adrenodoxin + H2O.

Cofactor

Heme group By similarity.

Subcellular location

Mitochondrion membrane.

Miscellaneous

The sequence shown is that of isozyme 11-beta-2.

Sequence similarities

Belongs to the cytochrome P450 family.

Sequence caution

The sequence BAA00127.1 differs from that shown. Reason: Frameshift at positions 186 and 192.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Transit peptide1 – 2424Mitochondrion
Chain25 – 503479Cytochrome P450 11B1, mitochondrial
PRO_0000003594

Sites

Metal binding4501Iron (heme axial ligand)

Natural variations

Natural variant301A → V in 11-beta-3.
Natural variant601S → G in 11-beta-3.
Natural variant1061H → R in 11-beta-3.

Experimental info

Sequence conflict191 – 1922SV → RL in AAA83383. Ref.3
Sequence conflict3371Q → T in BAA00347. Ref.4
Sequence conflict3371Q → T no nucleotide entry Ref.5
Sequence conflict3471A → P in AAA83383. Ref.3
Sequence conflict5021I → Y in BAA00347. Ref.4

Sequences

Sequence LengthMass (Da)Tools
P15150 [UniParc].

Last modified November 1, 1990. Version 2.
Checksum: 9FBEEC132975FD72

FASTA50357,847
        10         20         30         40         50         60 
MALWAKARVR MAGPWLSLHE ARLLGTRGAA APKAVLPFEA MPRCPGNKWM RMLQIWKEQS 

        70         80         90        100        110        120 
SENMHLDMHQ TFQELGPIFR YDVGGRHMVF VMLPEDVERL QQADSHHPQR MILEPWLAYR 

       130        140        150        160        170        180 
QARGHKCGVF LLNGPQWRLD RLRLNPDVLS LPALQKYTPL VDGVARDFSQ TLKARVLQNA 

       190        200        210        220        230        240 
RGSLTLDIAP SVFRYTIEAS TLVLYGERLG LLTQQPNPDS LNFIHALEAM LKSTVQLMFV 

       250        260        270        280        290        300 
PRRLSRWMST NMWREHFEAW DYIFQYANRA IQRIYQELAL GHPWHYSGIV AELLMRADMT 

       310        320        330        340        350        360 
LDTIKANTID LTAGSVDTTA FPLLMTLFEL ARNPEVQQAV RQESLVAEAR ISENPQRAIT 

       370        380        390        400        410        420 
ELPLLRAALK ETLRLYPVGI TLEREVSSDL VLQNYHIPAG TLVKVLLYSL GRNPAVFARP 

       430        440        450        460        470        480 
ESYHPQRWLD RQGSGSRFPH LAFGFGVRQC LGRRVAEVEM LLLLHHVLKN FLVETLEQED 

       490        500 
IKMVYRFILM PSTLPLFTFR AIQ 

« Hide

References

[1]"Molecular cloning and nucleotide sequence of DNA of mitochondrial cytochrome P-450(11 beta) of bovine adrenal cortex."
Morohashi K., Yoshioka H., Gotoh O., Okada Y., Yamamoto K., Miyata T., Sogawa K., Fujii-Kuriyama Y., Omura T.
J. Biochem. 102:559-568(1987) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOZYME-2), PARTIAL PROTEIN SEQUENCE.
[2]"Expression of two kinds of cytochrome P-450(11 beta) mRNA in bovine adrenal cortex."
Kirita S., Morohashi K., Hashimoto T., Yoshioka H., Fujii-Kuriyama Y., Omura T.
J. Biochem. 104:683-686(1988) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOZYME-3).
Tissue: Adrenal cortex.
[3]"Cloning of cDNA encoding steroid 11 beta-hydroxylase (P450c11)."
Chua S.C., Szabo P., Vitek A., Grzeschik K.H., John M., White P.C.
Proc. Natl. Acad. Sci. U.S.A. 84:7193-7197(1987) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA].
[4]"Cloning and characterization of bovine cytochrome P-450(11 beta) genes."
Hashimoto T., Morohashi K., Omura T.
J. Biochem. 105:676-679(1989) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
[5]"Structural analysis of multiple bovine P-450(11 beta) genes and their promoter activities."
Kirita S., Hashimoto T., Kitajima M., Honda S., Morohashi K., Omura T.
J. Biochem. 108:1030-1041(1990) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
D00185 mRNA. Translation: BAA00127.1. Frameshift.
D00361 mRNA. Translation: BAA00268.1.
M17843 mRNA. Translation: AAA83383.1.
D00455 Genomic DNA. Translation: BAA00347.1.
PIRA28415.
JX0071.
JX0151.
RefSeqNP_777063.2. NM_174638.3.
UniGeneBt.4297.

3D structure databases

ProteinModelPortalP15150.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

STRING9913.ENSBTAP00000037225.

Chemistry

BindingDBP15150.
ChEMBLCHEMBL2927.

Proteomic databases

PRIDEP15150.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

GeneID282422.
KEGGbta:282422.

Organism-specific databases

CTD1584.

Phylogenomic databases

eggNOGCOG2124.
HOGENOMHOG000013161.
HOVERGENHBG051098.
InParanoidP15150.
KOK00497.

Family and domain databases

Gene3D1.10.630.10. 1 hit.
InterProIPR001128. Cyt_P450.
IPR017972. Cyt_P450_CS.
IPR002401. Cyt_P450_E_grp-I.
[Graphical view]
PfamPF00067. p450. 1 hit.
[Graphical view]
PRINTSPR00463. EP450I.
PR00385. P450.
SUPFAMSSF48264. SSF48264. 1 hit.
PROSITEPS00086. CYTOCHROME_P450. 1 hit.
[Graphical view]
ProtoNetSearch...

Other

NextBio20806202.

Entry information

Entry nameC11B1_BOVIN
AccessionPrimary (citable) accession number: P15150
Secondary accession number(s): Q29457
Entry history
Integrated into UniProtKB/Swiss-Prot: April 1, 1990
Last sequence update: November 1, 1990
Last modified: January 22, 2014
This is version 113 of the entry and version 2 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families