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P15149 (CP2A2_RAT) Reviewed, UniProtKB/Swiss-Prot

Last modified April 16, 2014. Version 116. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (1) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Cytochrome P450 2A2

EC=1.14.14.1
Alternative name(s):
CYPIIA2
Cytochrome P450-UT-4
Testosterone 15-alpha-hydroxylase
Gene names
Name:Cyp2a2
Synonyms:Cyp2a-2
OrganismRattus norvegicus (Rat) [Reference proteome]
Taxonomic identifier10116 [NCBI]
Taxonomic lineageEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeRattus

Protein attributes

Sequence length492 AA.
Sequence statusComplete.
Protein existenceEvidence at protein level

General annotation (Comments)

Function

Highly active in the 15-alpha-hydroxylation of testosterone.

Catalytic activity

RH + reduced flavoprotein + O2 = ROH + oxidized flavoprotein + H2O.

Cofactor

Heme group By similarity.

Subcellular location

Endoplasmic reticulum membrane; Peripheral membrane protein. Microsome membrane; Peripheral membrane protein.

Tissue specificity

Liver specific.

Sequence similarities

Belongs to the cytochrome P450 family.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 492492Cytochrome P450 2A2
PRO_0000051664

Sites

Metal binding4371Iron (heme axial ligand)

Experimental info

Sequence conflict41T → S AA sequence Ref.4

Sequences

Sequence LengthMass (Da)Tools
P15149 [UniParc].

Last modified April 1, 1990. Version 1.
Checksum: 9AB94CB68D34D81E

FASTA49256,345
        10         20         30         40         50         60 
MLDTGLLLVV ILASLSVMFL VSLWQQKIRE RLPPGPTPLP FIGNYLQLNM KDVYSSITQL 

        70         80         90        100        110        120 
SERYGPVFTI HLGPRRIVVL YGYDAVKEAL VDQAEEFSGR GELPTFNILF KGYGFSLSNV 

       130        140        150        160        170        180 
EQAKRIRRFT IATLRDFGVG KRDVQECILE EAGYLIKTLQ GTCGAPIDPS IYLSKTVSNV 

       190        200        210        220        230        240 
INSIVFGNRF DYEDKEFLSL LEMIDEMNIF AASATGQLYD MFHSVMKYLP GPQQQIIKVT 

       250        260        270        280        290        300 
QKLEDFMIEK VRQNHSTLDP NSPRNFIDSF LIRMQEEKYV NSEFHMNNLV MSSLGLLFAG 

       310        320        330        340        350        360 
TGSVSSTLYH GFLLLMKHPD VEAKVHEEIE RVIGRNRQPQ YEDHMKMPYT QAVINEIQRF 

       370        380        390        400        410        420 
SNLAPLGIPR RIIKNTTFRG FFLPKGTDVF PIIGSLMTEP KFFPNHKDFN PQHFLDDKGQ 

       430        440        450        460        470        480 
LKKNAAFLPF SIGKRFCLGD SLAKMELFLL LTTILQNFRF KFPMNLEDIN EYPSPIGFTR 

       490 
IIPNYTMSFM PI 

« Hide

References

« Hide 'large scale' references
[1]"Gene conversion and differential regulation in the rat P-450 IIA gene subfamily. Purification, catalytic activity, cDNA and deduced amino acid sequence, and regulation of an adult male-specific hepatic testosterone 15 alpha-hydroxylase."
Matsunaga T., Nagata K., Holsztynska E.J., Lapenson D.P., Smith A., Kato R., Gelboin H.V., Waxman D.J., Gonzalez F.J.
J. Biol. Chem. 263:17995-18002(1988) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA].
Tissue: Liver.
[2]"Structure and in vitro transcription of the rat CYP2A1 and CYP2A2 genes and regional localization of the CYP2A gene subfamily on mouse chromosome 7."
Matsunaga T., Nomoto M., Kozak C.A., Gonzalez F.J.
Biochemistry 29:1329-1341(1990) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
[3]"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
The MGC Project Team
Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
Tissue: Liver.
[4]"Purification and characterization of three male-specific and one female-specific forms of cytochrome P-450 from rat liver microsomes."
Matsumoto T., Emi Y., Kawabata S., Omura T.
J. Biochem. 100:1359-1371(1986) [PubMed] [Europe PMC] [Abstract]
Cited for: PROTEIN SEQUENCE OF 1-27.
Tissue: Liver.
[5]"Purification of two isozymes of rat liver microsomal cytochrome P450 with testosterone 7 alpha-hydroxylase activity."
Arlotto M.P., Greenway D.J., Parkinson A.
Arch. Biochem. Biophys. 270:441-457(1989) [PubMed] [Europe PMC] [Abstract]
Cited for: PROTEIN SEQUENCE OF 1-20.
Tissue: Liver.
+Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
J04187 mRNA. Translation: AAA41424.1.
M34392, M33313, M33325 Genomic DNA. Translation: AAA41021.1.
BC089818 mRNA. Translation: AAH89818.1.
PIRA31887. B34272.
RefSeqNP_036825.1. NM_012693.1.
UniGeneRn.9867.

3D structure databases

ProteinModelPortalP15149.
SMRP15149. Positions 31-491.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

MINTMINT-4567801.
STRING10116.ENSRNOP00000028249.

Chemistry

BindingDBP15149.
ChEMBLCHEMBL3705.

Proteomic databases

PaxDbP15149.
PRIDEP15149.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

GeneID24895.
KEGGrno:24895.

Organism-specific databases

CTD24895.
RGD2464. Cyp2a2.

Phylogenomic databases

eggNOGCOG2124.
HOGENOMHOG000036992.
HOVERGENHBG015789.
InParanoidP15149.
KOK07411.
OrthoDBEOG7RBZ85.
PhylomeDBP15149.
TreeFamTF352043.

Gene expression databases

GenevestigatorP15149.

Family and domain databases

Gene3D1.10.630.10. 1 hit.
InterProIPR001128. Cyt_P450.
IPR017972. Cyt_P450_CS.
IPR002401. Cyt_P450_E_grp-I.
IPR008067. Cyt_P450_E_grp-I_CYP2A-like.
[Graphical view]
PfamPF00067. p450. 1 hit.
[Graphical view]
PRINTSPR00463. EP450I.
PR01684. EP450ICYP2A.
PR00385. P450.
SUPFAMSSF48264. SSF48264. 1 hit.
PROSITEPS00086. CYTOCHROME_P450. 1 hit.
[Graphical view]
ProtoNetSearch...

Other

NextBio604770.

Entry information

Entry nameCP2A2_RAT
AccessionPrimary (citable) accession number: P15149
Secondary accession number(s): Q5EBB3
Entry history
Integrated into UniProtKB/Swiss-Prot: April 1, 1990
Last sequence update: April 1, 1990
Last modified: April 16, 2014
This is version 116 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families