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P15149

- CP2A2_RAT

UniProt

P15149 - CP2A2_RAT

Protein

Cytochrome P450 2A2

Gene

Cyp2a2

Organism
Rattus norvegicus (Rat)
Status
Reviewed - Annotation score: 4 out of 5- Experimental evidence at protein leveli
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    • History
      Entry version 117 (01 Oct 2014)
      Sequence version 1 (01 Apr 1990)
      Previous versions | rss
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    Functioni

    Highly active in the 15-alpha-hydroxylation of testosterone.

    Catalytic activityi

    RH + reduced flavoprotein + O2 = ROH + oxidized flavoprotein + H2O.

    Cofactori

    Heme group.By similarity

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Metal bindingi437 – 4371Iron (heme axial ligand)

    GO - Molecular functioni

    1. aromatase activity Source: UniProtKB-EC
    2. heme binding Source: InterPro
    3. iron ion binding Source: InterPro
    4. steroid hydroxylase activity Source: RGD

    Keywords - Molecular functioni

    Monooxygenase, Oxidoreductase

    Keywords - Ligandi

    Heme, Iron, Metal-binding

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Cytochrome P450 2A2 (EC:1.14.14.1)
    Alternative name(s):
    CYPIIA2
    Cytochrome P450-UT-4
    Testosterone 15-alpha-hydroxylase
    Gene namesi
    Name:Cyp2a2
    Synonyms:Cyp2a-2
    OrganismiRattus norvegicus (Rat)
    Taxonomic identifieri10116 [NCBI]
    Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeRattus
    ProteomesiUP000002494: Unplaced

    Organism-specific databases

    RGDi2464. Cyp2a2.

    Subcellular locationi

    GO - Cellular componenti

    1. endoplasmic reticulum membrane Source: UniProtKB-SubCell

    Keywords - Cellular componenti

    Endoplasmic reticulum, Membrane, Microsome

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 492492Cytochrome P450 2A2PRO_0000051664Add
    BLAST

    Proteomic databases

    PaxDbiP15149.
    PRIDEiP15149.

    Expressioni

    Tissue specificityi

    Liver specific.

    Gene expression databases

    GenevestigatoriP15149.

    Interactioni

    Protein-protein interaction databases

    MINTiMINT-4567801.
    STRINGi10116.ENSRNOP00000028249.

    Structurei

    3D structure databases

    ProteinModelPortaliP15149.
    SMRiP15149. Positions 31-491.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Sequence similaritiesi

    Belongs to the cytochrome P450 family.Curated

    Phylogenomic databases

    eggNOGiCOG2124.
    HOGENOMiHOG000036992.
    HOVERGENiHBG015789.
    InParanoidiP15149.
    KOiK07411.
    OrthoDBiEOG7RBZ85.
    PhylomeDBiP15149.
    TreeFamiTF352043.

    Family and domain databases

    Gene3Di1.10.630.10. 1 hit.
    InterProiIPR001128. Cyt_P450.
    IPR017972. Cyt_P450_CS.
    IPR002401. Cyt_P450_E_grp-I.
    IPR008067. Cyt_P450_E_grp-I_CYP2A-like.
    [Graphical view]
    PfamiPF00067. p450. 1 hit.
    [Graphical view]
    PRINTSiPR00463. EP450I.
    PR01684. EP450ICYP2A.
    PR00385. P450.
    SUPFAMiSSF48264. SSF48264. 1 hit.
    PROSITEiPS00086. CYTOCHROME_P450. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    P15149-1 [UniParc]FASTAAdd to Basket

    « Hide

    MLDTGLLLVV ILASLSVMFL VSLWQQKIRE RLPPGPTPLP FIGNYLQLNM    50
    KDVYSSITQL SERYGPVFTI HLGPRRIVVL YGYDAVKEAL VDQAEEFSGR 100
    GELPTFNILF KGYGFSLSNV EQAKRIRRFT IATLRDFGVG KRDVQECILE 150
    EAGYLIKTLQ GTCGAPIDPS IYLSKTVSNV INSIVFGNRF DYEDKEFLSL 200
    LEMIDEMNIF AASATGQLYD MFHSVMKYLP GPQQQIIKVT QKLEDFMIEK 250
    VRQNHSTLDP NSPRNFIDSF LIRMQEEKYV NSEFHMNNLV MSSLGLLFAG 300
    TGSVSSTLYH GFLLLMKHPD VEAKVHEEIE RVIGRNRQPQ YEDHMKMPYT 350
    QAVINEIQRF SNLAPLGIPR RIIKNTTFRG FFLPKGTDVF PIIGSLMTEP 400
    KFFPNHKDFN PQHFLDDKGQ LKKNAAFLPF SIGKRFCLGD SLAKMELFLL 450
    LTTILQNFRF KFPMNLEDIN EYPSPIGFTR IIPNYTMSFM PI 492
    Length:492
    Mass (Da):56,345
    Last modified:April 1, 1990 - v1
    Checksum:i9AB94CB68D34D81E
    GO

    Experimental Info

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Sequence conflicti4 – 41T → S AA sequence (PubMed:2434473)Curated

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    J04187 mRNA. Translation: AAA41424.1.
    M34392, M33313, M33325 Genomic DNA. Translation: AAA41021.1.
    BC089818 mRNA. Translation: AAH89818.1.
    PIRiB34272. A31887.
    RefSeqiNP_036825.1. NM_012693.1.
    UniGeneiRn.9867.

    Genome annotation databases

    GeneIDi24895.
    KEGGirno:24895.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    J04187 mRNA. Translation: AAA41424.1 .
    M34392 , M33313 , M33325 Genomic DNA. Translation: AAA41021.1 .
    BC089818 mRNA. Translation: AAH89818.1 .
    PIRi B34272. A31887.
    RefSeqi NP_036825.1. NM_012693.1.
    UniGenei Rn.9867.

    3D structure databases

    ProteinModelPortali P15149.
    SMRi P15149. Positions 31-491.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    MINTi MINT-4567801.
    STRINGi 10116.ENSRNOP00000028249.

    Chemistry

    BindingDBi P15149.
    ChEMBLi CHEMBL3705.

    Proteomic databases

    PaxDbi P15149.
    PRIDEi P15149.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    GeneIDi 24895.
    KEGGi rno:24895.

    Organism-specific databases

    CTDi 24895.
    RGDi 2464. Cyp2a2.

    Phylogenomic databases

    eggNOGi COG2124.
    HOGENOMi HOG000036992.
    HOVERGENi HBG015789.
    InParanoidi P15149.
    KOi K07411.
    OrthoDBi EOG7RBZ85.
    PhylomeDBi P15149.
    TreeFami TF352043.

    Miscellaneous databases

    NextBioi 604770.

    Gene expression databases

    Genevestigatori P15149.

    Family and domain databases

    Gene3Di 1.10.630.10. 1 hit.
    InterProi IPR001128. Cyt_P450.
    IPR017972. Cyt_P450_CS.
    IPR002401. Cyt_P450_E_grp-I.
    IPR008067. Cyt_P450_E_grp-I_CYP2A-like.
    [Graphical view ]
    Pfami PF00067. p450. 1 hit.
    [Graphical view ]
    PRINTSi PR00463. EP450I.
    PR01684. EP450ICYP2A.
    PR00385. P450.
    SUPFAMi SSF48264. SSF48264. 1 hit.
    PROSITEi PS00086. CYTOCHROME_P450. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "Gene conversion and differential regulation in the rat P-450 IIA gene subfamily. Purification, catalytic activity, cDNA and deduced amino acid sequence, and regulation of an adult male-specific hepatic testosterone 15 alpha-hydroxylase."
      Matsunaga T., Nagata K., Holsztynska E.J., Lapenson D.P., Smith A., Kato R., Gelboin H.V., Waxman D.J., Gonzalez F.J.
      J. Biol. Chem. 263:17995-18002(1988) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [MRNA].
      Tissue: Liver.
    2. "Structure and in vitro transcription of the rat CYP2A1 and CYP2A2 genes and regional localization of the CYP2A gene subfamily on mouse chromosome 7."
      Matsunaga T., Nomoto M., Kozak C.A., Gonzalez F.J.
      Biochemistry 29:1329-1341(1990) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
    3. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
      The MGC Project Team
      Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
      Tissue: Liver.
    4. "Purification and characterization of three male-specific and one female-specific forms of cytochrome P-450 from rat liver microsomes."
      Matsumoto T., Emi Y., Kawabata S., Omura T.
      J. Biochem. 100:1359-1371(1986) [PubMed] [Europe PMC] [Abstract]
      Cited for: PROTEIN SEQUENCE OF 1-27.
      Tissue: Liver.
    5. "Purification of two isozymes of rat liver microsomal cytochrome P450 with testosterone 7 alpha-hydroxylase activity."
      Arlotto M.P., Greenway D.J., Parkinson A.
      Arch. Biochem. Biophys. 270:441-457(1989) [PubMed] [Europe PMC] [Abstract]
      Cited for: PROTEIN SEQUENCE OF 1-20.
      Tissue: Liver.

    Entry informationi

    Entry nameiCP2A2_RAT
    AccessioniPrimary (citable) accession number: P15149
    Secondary accession number(s): Q5EBB3
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: April 1, 1990
    Last sequence update: April 1, 1990
    Last modified: October 1, 2014
    This is version 117 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programChordata Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Direct protein sequencing, Reference proteome

    Documents

    1. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3