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P15149

- CP2A2_RAT

UniProt

P15149 - CP2A2_RAT

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Protein

Cytochrome P450 2A2

Gene

Cyp2a2

Organism
Rattus norvegicus (Rat)
Status
Reviewed - Annotation score: 4 out of 5- Experimental evidence at protein leveli

Functioni

Highly active in the 15-alpha-hydroxylation of testosterone.

Catalytic activityi

RH + reduced flavoprotein + O2 = ROH + oxidized flavoprotein + H2O.

Cofactori

Heme group.By similarity

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Metal bindingi437 – 4371Iron (heme axial ligand)

GO - Molecular functioni

  1. aromatase activity Source: UniProtKB-EC
  2. heme binding Source: InterPro
  3. iron ion binding Source: InterPro
  4. steroid hydroxylase activity Source: RGD
Complete GO annotation...

Keywords - Molecular functioni

Monooxygenase, Oxidoreductase

Keywords - Ligandi

Heme, Iron, Metal-binding

Names & Taxonomyi

Protein namesi
Recommended name:
Cytochrome P450 2A2 (EC:1.14.14.1)
Alternative name(s):
CYPIIA2
Cytochrome P450-UT-4
Testosterone 15-alpha-hydroxylase
Gene namesi
Name:Cyp2a2
Synonyms:Cyp2a-2
OrganismiRattus norvegicus (Rat)
Taxonomic identifieri10116 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeRattus
ProteomesiUP000002494: Unplaced

Organism-specific databases

RGDi2464. Cyp2a2.

Subcellular locationi

GO - Cellular componenti

  1. endoplasmic reticulum Source: UniProtKB-KW
  2. membrane Source: UniProtKB-KW
Complete GO annotation...

Keywords - Cellular componenti

Endoplasmic reticulum, Membrane, Microsome

Pathology & Biotechi

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 492492Cytochrome P450 2A2PRO_0000051664Add
BLAST

Proteomic databases

PaxDbiP15149.
PRIDEiP15149.

Expressioni

Tissue specificityi

Liver specific.

Gene expression databases

GenevestigatoriP15149.

Interactioni

Protein-protein interaction databases

MINTiMINT-4567801.
STRINGi10116.ENSRNOP00000028249.

Structurei

3D structure databases

ProteinModelPortaliP15149.
SMRiP15149. Positions 31-491.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the cytochrome P450 family.Curated

Phylogenomic databases

eggNOGiCOG2124.
HOGENOMiHOG000036992.
HOVERGENiHBG015789.
InParanoidiP15149.
KOiK07411.
OrthoDBiEOG7RBZ85.
PhylomeDBiP15149.
TreeFamiTF352043.

Family and domain databases

Gene3Di1.10.630.10. 1 hit.
InterProiIPR001128. Cyt_P450.
IPR017972. Cyt_P450_CS.
IPR002401. Cyt_P450_E_grp-I.
IPR008067. Cyt_P450_E_grp-I_CYP2A-like.
[Graphical view]
PfamiPF00067. p450. 1 hit.
[Graphical view]
PRINTSiPR00463. EP450I.
PR01684. EP450ICYP2A.
PR00385. P450.
SUPFAMiSSF48264. SSF48264. 1 hit.
PROSITEiPS00086. CYTOCHROME_P450. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

P15149 [UniParc]FASTAAdd to Basket

« Hide

        10         20         30         40         50
MLDTGLLLVV ILASLSVMFL VSLWQQKIRE RLPPGPTPLP FIGNYLQLNM
60 70 80 90 100
KDVYSSITQL SERYGPVFTI HLGPRRIVVL YGYDAVKEAL VDQAEEFSGR
110 120 130 140 150
GELPTFNILF KGYGFSLSNV EQAKRIRRFT IATLRDFGVG KRDVQECILE
160 170 180 190 200
EAGYLIKTLQ GTCGAPIDPS IYLSKTVSNV INSIVFGNRF DYEDKEFLSL
210 220 230 240 250
LEMIDEMNIF AASATGQLYD MFHSVMKYLP GPQQQIIKVT QKLEDFMIEK
260 270 280 290 300
VRQNHSTLDP NSPRNFIDSF LIRMQEEKYV NSEFHMNNLV MSSLGLLFAG
310 320 330 340 350
TGSVSSTLYH GFLLLMKHPD VEAKVHEEIE RVIGRNRQPQ YEDHMKMPYT
360 370 380 390 400
QAVINEIQRF SNLAPLGIPR RIIKNTTFRG FFLPKGTDVF PIIGSLMTEP
410 420 430 440 450
KFFPNHKDFN PQHFLDDKGQ LKKNAAFLPF SIGKRFCLGD SLAKMELFLL
460 470 480 490
LTTILQNFRF KFPMNLEDIN EYPSPIGFTR IIPNYTMSFM PI
Length:492
Mass (Da):56,345
Last modified:April 1, 1990 - v1
Checksum:i9AB94CB68D34D81E
GO

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti4 – 41T → S AA sequence (PubMed:2434473)Curated

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
J04187 mRNA. Translation: AAA41424.1.
M34392, M33313, M33325 Genomic DNA. Translation: AAA41021.1.
BC089818 mRNA. Translation: AAH89818.1.
PIRiB34272. A31887.
RefSeqiNP_036825.1. NM_012693.1.
UniGeneiRn.9867.

Genome annotation databases

GeneIDi24895.
KEGGirno:24895.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
J04187 mRNA. Translation: AAA41424.1 .
M34392 , M33313 , M33325 Genomic DNA. Translation: AAA41021.1 .
BC089818 mRNA. Translation: AAH89818.1 .
PIRi B34272. A31887.
RefSeqi NP_036825.1. NM_012693.1.
UniGenei Rn.9867.

3D structure databases

ProteinModelPortali P15149.
SMRi P15149. Positions 31-491.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

MINTi MINT-4567801.
STRINGi 10116.ENSRNOP00000028249.

Chemistry

BindingDBi P15149.
ChEMBLi CHEMBL3705.
DrugBanki DB08834. Tauroursodeoxycholic acid.

Proteomic databases

PaxDbi P15149.
PRIDEi P15149.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

GeneIDi 24895.
KEGGi rno:24895.

Organism-specific databases

CTDi 24895.
RGDi 2464. Cyp2a2.

Phylogenomic databases

eggNOGi COG2124.
HOGENOMi HOG000036992.
HOVERGENi HBG015789.
InParanoidi P15149.
KOi K07411.
OrthoDBi EOG7RBZ85.
PhylomeDBi P15149.
TreeFami TF352043.

Miscellaneous databases

NextBioi 604770.

Gene expression databases

Genevestigatori P15149.

Family and domain databases

Gene3Di 1.10.630.10. 1 hit.
InterProi IPR001128. Cyt_P450.
IPR017972. Cyt_P450_CS.
IPR002401. Cyt_P450_E_grp-I.
IPR008067. Cyt_P450_E_grp-I_CYP2A-like.
[Graphical view ]
Pfami PF00067. p450. 1 hit.
[Graphical view ]
PRINTSi PR00463. EP450I.
PR01684. EP450ICYP2A.
PR00385. P450.
SUPFAMi SSF48264. SSF48264. 1 hit.
PROSITEi PS00086. CYTOCHROME_P450. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

« Hide 'large scale' publications
  1. "Gene conversion and differential regulation in the rat P-450 IIA gene subfamily. Purification, catalytic activity, cDNA and deduced amino acid sequence, and regulation of an adult male-specific hepatic testosterone 15 alpha-hydroxylase."
    Matsunaga T., Nagata K., Holsztynska E.J., Lapenson D.P., Smith A., Kato R., Gelboin H.V., Waxman D.J., Gonzalez F.J.
    J. Biol. Chem. 263:17995-18002(1988) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA].
    Tissue: Liver.
  2. "Structure and in vitro transcription of the rat CYP2A1 and CYP2A2 genes and regional localization of the CYP2A gene subfamily on mouse chromosome 7."
    Matsunaga T., Nomoto M., Kozak C.A., Gonzalez F.J.
    Biochemistry 29:1329-1341(1990) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
  3. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
    The MGC Project Team
    Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
    Tissue: Liver.
  4. "Purification and characterization of three male-specific and one female-specific forms of cytochrome P-450 from rat liver microsomes."
    Matsumoto T., Emi Y., Kawabata S., Omura T.
    J. Biochem. 100:1359-1371(1986) [PubMed] [Europe PMC] [Abstract]
    Cited for: PROTEIN SEQUENCE OF 1-27.
    Tissue: Liver.
  5. "Purification of two isozymes of rat liver microsomal cytochrome P450 with testosterone 7 alpha-hydroxylase activity."
    Arlotto M.P., Greenway D.J., Parkinson A.
    Arch. Biochem. Biophys. 270:441-457(1989) [PubMed] [Europe PMC] [Abstract]
    Cited for: PROTEIN SEQUENCE OF 1-20.
    Tissue: Liver.

Entry informationi

Entry nameiCP2A2_RAT
AccessioniPrimary (citable) accession number: P15149
Secondary accession number(s): Q5EBB3
Entry historyi
Integrated into UniProtKB/Swiss-Prot: April 1, 1990
Last sequence update: April 1, 1990
Last modified: October 29, 2014
This is version 118 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Direct protein sequencing, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3