Reviewed, UniProtKB/Swiss-Prot P15149 (CP2A2_RAT)

Names and origin

Protein names

Recommended name:
    Cytochrome P450 2A2
    EC=1.14.14.1
Alternative name(s):
    CYPIIA2
    Testosterone 15-alpha-hydroxylase
    P450-UT-4

Gene names

Name:	Cyp2a2
Synonyms:	Cyp2a-2

Organism

Rattus norvegicus (Rat)

Taxonomic identifier

10116 [NCBI]

Taxonomic lineage

Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Glires › Rodentia › Sciurognathi › Muroidea › Muridae › Murinae › Rattus

Protein attributes

Sequence length	492 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Evidence at protein level.

General annotation (Comments)

Function	Highly active in the 15-alpha-hydroxylation of testosterone.
Catalytic activity	RH + reduced flavoprotein + O2 = ROH + oxidized flavoprotein + H2O.
Cofactor	Heme group By similarity.
Subcellular location	Endoplasmic reticulum membrane; Peripheral membrane protein. Microsome membrane; Peripheral membrane protein.
Tissue specificity	Liver specific.
Sequence similarities	Belongs to the cytochrome P450 family.

Ontologies

Keywords
Cellular component	Endoplasmic reticulum Membrane Microsome
Ligand	Heme Iron Metal-binding
Molecular function	Monooxygenase Oxidoreductase
Technical term	Direct protein sequencing
Gene Ontology (GO)
Biological process	oxidation reduction Inferred from electronic annotation. Source: UniProtKB-KW
Cellular component	endoplasmic reticulum membrane Inferred from electronic annotation. Source: UniProtKB-SubCell microsome Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	aromatase activity Inferred from electronic annotation. Source: EC electron carrier activity Inferred from electronic annotation. Source: InterPro heme binding Inferred from electronic annotation. Source: InterPro steroid hydroxylase activity Ref.1 Ref.2 Inferred from direct assay. Source: RGD
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 492	492	Cytochrome P450 2A2		PRO_0000051664
Sites
	Metal binding	437	1	Iron (heme axial ligand)
Experimental info
	Sequence conflict	4	1	T → S AA sequence Ref.4

Sequences

	Sequence		Length	Mass (Da)
	P15149-1 [UniParc]. Last modified April 1, 1990. Version 1. Checksum: 9AB94CB68D34D81E	FASTA	492	56,345
	10 20 30 40 50 60 MLDTGLLLVV ILASLSVMFL VSLWQQKIRE RLPPGPTPLP FIGNYLQLNM KDVYSSITQL 70 80 90 100 110 120 SERYGPVFTI HLGPRRIVVL YGYDAVKEAL VDQAEEFSGR GELPTFNILF KGYGFSLSNV 130 140 150 160 170 180 EQAKRIRRFT IATLRDFGVG KRDVQECILE EAGYLIKTLQ GTCGAPIDPS IYLSKTVSNV 190 200 210 220 230 240 INSIVFGNRF DYEDKEFLSL LEMIDEMNIF AASATGQLYD MFHSVMKYLP GPQQQIIKVT 250 260 270 280 290 300 QKLEDFMIEK VRQNHSTLDP NSPRNFIDSF LIRMQEEKYV NSEFHMNNLV MSSLGLLFAG 310 320 330 340 350 360 TGSVSSTLYH GFLLLMKHPD VEAKVHEEIE RVIGRNRQPQ YEDHMKMPYT QAVINEIQRF 370 380 390 400 410 420 SNLAPLGIPR RIIKNTTFRG FFLPKGTDVF PIIGSLMTEP KFFPNHKDFN PQHFLDDKGQ 430 440 450 460 470 480 LKKNAAFLPF SIGKRFCLGD SLAKMELFLL LTTILQNFRF KFPMNLEDIN EYPSPIGFTR 490 IIPNYTMSFM PI

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Gene conversion and differential regulation in the rat P-450 IIA gene subfamily. Purification, catalytic activity, cDNA and deduced amino acid sequence, and regulation of an adult male-specific hepatic testosterone 15 alpha-hydroxylase." Matsunaga T., Nagata K., Holsztynska E.J., Lapenson D.P., Smith A., Kato R., Gelboin H.V., Waxman D.J., Gonzalez F.J. J. Biol. Chem. 263:17995-18002(1988) [PubMed: 3192524] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA]. Tissue: Liver.
[2]	"Structure and in vitro transcription of the rat CYP2A1 and CYP2A2 genes and regional localization of the CYP2A gene subfamily on mouse chromosome 7." Matsunaga T., Nomoto M., Kozak C.A., Gonzalez F.J. Biochemistry 29:1329-1341(1990) [PubMed: 2322568] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
[3]	"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)." The MGC Project Team Genome Res. 14:2121-2127(2004) [PubMed: 15489334] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. Tissue: Liver.
[4]	"Purification and characterization of three male-specific and one female-specific forms of cytochrome P-450 from rat liver microsomes." Matsumoto T., Emi Y., Kawabata S., Omura T. J. Biochem. 100:1359-1371(1986) [PubMed: 2434473] [Abstract] Cited for: PROTEIN SEQUENCE OF 1-27. Tissue: Liver.
[5]	"Purification of two isozymes of rat liver microsomal cytochrome P450 with testosterone 7 alpha-hydroxylase activity." Arlotto M.P., Greenway D.J., Parkinson A. Arch. Biochem. Biophys. 270:441-457(1989) [PubMed: 2650624] [Abstract] Cited for: PROTEIN SEQUENCE OF 1-20. Tissue: Liver.

Cross-references

Sequence databases
EMBLGenBankDDBJ	J04187 mRNA. Translation: AAA41424.1. M34392, M33313, M33325 Genomic DNA. Translation: AAA41021.1. BC089818 mRNA. Translation: AAH89818.1.
IPI	IPI00206174.
PIR	A31887. B34272.
RefSeq	NP_036825.1.
UniGene	Rn.9867
3D structure databases
HSSP	HSSP built from PDB template 1DT6 based on UniProtKB P00179.
ModBase	Search...
Proteomic databases
PRIDE	P15149.
Genome annotation databases
Ensembl	ENSRNOG00000020817. Rattus norvegicus. [Contig view]
GeneID	24895.
KEGG	rno:24895.
Organism-specific databases
RGD	2464. Cyp2a2.
Phylogenomic databases
HOVERGEN	P15149.
Enzyme and pathway databases
BRENDA	1.14.14.1. 248.
Gene expression databases
ArrayExpress	P15149.
GermOnline	ENSRNOG00000020817. Rattus norvegicus.
Family and domain databases
InterPro	IPR001128. Cyt_P450. IPR017973. Cyt_P450_C. IPR017972. Cyt_P450_CS. IPR002401. Cyt_P450_E_grp-I. IPR008067. Cyt_P450_E_grp-I_CYP2A-like. [Graphical view]
Gene3D	G3DSA:1.10.630.10. Cyt_P450. 1 hit.
PANTHER	PTHR19383. Cyt_P450. 1 hit.
Pfam	PF00067. p450. 1 hit. [Graphical view]
PRINTS	PR00463. EP450I. PR01684. EP450ICYP2A. PR00385. P450.
PROSITE	PS00086. CYTOCHROME_P450. 1 hit. [Graphical view]
ProtoNet	Search...
Other Resources
NextBio	604770.

Entry information

Entry name

CP2A2_RAT

Accession

Primary (citable) accession number: P15149
Secondary accession number(s): Q5EBB3

Entry history

Integrated into UniProtKB/Swiss-Prot:	April 1, 1990
Last sequence update:	April 1, 1990
Last modified:	June 16, 2009
This is version 81 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HPI (Human Proteome Initiative)