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P15146

- MTAP2_RAT

UniProt

P15146 - MTAP2_RAT

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Protein

Microtubule-associated protein 2

Gene

Map2

Organism
Rattus norvegicus (Rat)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at protein leveli

Functioni

The exact function of MAP2 is unknown but MAPs may stabilize the microtubules against depolymerization. They also seem to have a stiffening effect on microtubules.

GO - Biological processi

  1. response to estradiol Source: RGD
  2. response to progesterone Source: RGD
Complete GO annotation...

Keywords - Ligandi

Calmodulin-binding

Names & Taxonomyi

Protein namesi
Recommended name:
Microtubule-associated protein 2
Short name:
MAP-2
Gene namesi
Name:Map2
Synonyms:Mtap2
OrganismiRattus norvegicus (Rat)
Taxonomic identifieri10116 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeRattus
ProteomesiUP000002494: Unplaced

Organism-specific databases

RGDi3044. Map2.

Subcellular locationi

GO - Cellular componenti

  1. dendrite Source: BHF-UCL
  2. microtubule Source: UniProtKB-KW
  3. neuronal cell body Source: RGD
  4. neuron projection Source: RGD
  5. protein complex Source: RGD
  6. rough endoplasmic reticulum Source: RGD
  7. smooth endoplasmic reticulum Source: RGD
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm, Cytoskeleton, Microtubule

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 18611861Microtubule-associated protein 2PRO_0000072749Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei728 – 7281PhosphoserineBy similarity
Modified residuei1353 – 13531PhosphoserineBy similarity
Modified residuei1359 – 13591PhosphothreonineBy similarity
Modified residuei1608 – 16081PhosphothreonineBy similarity
Modified residuei1611 – 16111PhosphothreonineBy similarity
Modified residuei1682 – 16821Phosphoserine; by MARK11 Publication

Post-translational modificationi

Phosphorylated at serine residues in K-X-G-S motifs by MAP/microtubule affinity-regulating kinase (MARK1 or MARK2), causing detachment from microtubules, and their disassembly. Isoform 3/MAP2c is probably phosphorylated by PKA at Ser-319, Ser-350 and Ser-382 and by FYN at Tyr-67.1 Publication

Keywords - PTMi

Phosphoprotein

Proteomic databases

PaxDbiP15146.
PRIDEiP15146.

PTM databases

PhosphoSiteiP15146.

Expressioni

Developmental stagei

Isoform 3/MAP2c is expressed during embryonic brain development and until postanatal day 10. Isoform 2 is expressed throughout brain development.

Gene expression databases

GenevestigatoriP15146.

Interactioni

Protein-protein interaction databases

IntActiP15146. 4 interactions.
MINTiMINT-261227.

Structurei

3D structure databases

DisProtiDP00122.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Repeati1664 – 169431Tau/MAP 1Add
BLAST
Repeati1695 – 172531Tau/MAP 2Add
BLAST
Repeati1726 – 175631Tau/MAP 3Add
BLAST
Repeati1757 – 178832Tau/MAP 4Add
BLAST

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni1454 – 147421Calmodulin-bindingSequence AnalysisAdd
BLAST

Sequence similaritiesi

Contains 4 Tau/MAP repeats.PROSITE-ProRule annotation

Keywords - Domaini

Repeat

Phylogenomic databases

eggNOGiNOG148882.
HOGENOMiHOG000113477.
HOVERGENiHBG006322.
InParanoidiP15146.
KOiK10430.
PhylomeDBiP15146.

Family and domain databases

InterProiIPR027324. MAP2/MAP4/Tau.
IPR013588. MAP2_projctn.
IPR001084. MAP_tubulin-bd_rpt.
[Graphical view]
PANTHERiPTHR11501. PTHR11501. 1 hit.
PfamiPF08377. MAP2_projctn. 1 hit.
PF00418. Tubulin-binding. 4 hits.
[Graphical view]
PROSITEiPS00229. TAU_MAP_1. 3 hits.
PS51491. TAU_MAP_2. 4 hits.
[Graphical view]

Sequences (4)i

Sequence statusi: Complete.

This entry describes 4 isoformsi produced by alternative splicing. Align

Note: Additional isoforms seem to exist.

Isoform 1 (identifier: P15146-1) [UniParc]FASTAAdd to Basket

Also known as: MAP2x

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MADERKDEGK APHWTSASLT EAAAHPHSPE MKDQGGSGEG LSRSANGFPY
60 70 80 90 100
REEEEGAFGE HGSQGTYSDT KENGINGELT SADRETAEEV SARIVQVVTA
110 120 130 140 150
EAVAVLKGEQ EKEAQHKDQP AALPLAAEET VNLPPSPPPS PASEQTAALE
160 170 180 190 200
EDLLTASKME FPEQQKLPSS FAEPLDKEET EFKMQSKPGE DFEHAALVPQ
210 220 230 240 250
PDTSKTPQDK KDPQDMEGEK SPASPFAQTF GTNLEDIKQI TEPSITVPSI
260 270 280 290 300
GLSAEPLAPK DQKDWFIEMP VESKKDEWGL AAPISPGPLT PMREKDVLED
310 320 330 340 350
IPRWEGKQFD SPMPSPFHGG SFTLPLDTVK DERVTEGSQP FAPVFFQSDD
360 370 380 390 400
KMSLQDTSGS ATSKESSKDE EPQKDKADKV ADVPVSEATT VLGDVHSPAV
410 420 430 440 450
EGFVGENISG EEKGTTDQEK KETSTPSVQE PTLTETEPQT KLEETSKVSI
460 470 480 490 500
EETVAKEEES LKLKDDKAGV IQTSTEQSFS KEDQKGQEQT IEALKQDSFP
510 520 530 540 550
ISLEQAVTDA AMATKTLEKV TSEPEAVSEK REIQGLFEED IADKSKLEGA
560 570 580 590 600
GSATVAEVEM PFYEDKSGMS KYFETSALKE DVTRSTGLGS DYYELSDSRG
610 620 630 640 650
NAQESLDTVS PKNQQDEKEL LAKASQPSPP AHEAGYSTLA QSYTSDHPSE
660 670 680 690 700
LPEEPSSPQE RMFTIDPKVY GEKRDLHSKN KDDLTLSRSL GLGGRSAIEQ
710 720 730 740 750
RSMSINLPMS CLDSIALGFN FGRGHDLSPL ASDILTNTSG SMDEGDDYLP
760 770 780 790 800
PTTPAVEKIP CFPIESKEEE DKTEQAKVTG GQTTQVETSS ESPFPAKEYY
810 820 830 840 850
KNGTVMAPDL PEMLDLAGTR SRLASVSADA EVARRKSVPS EAVVAESSTG
860 870 880 890 900
LPPVADDSQP VKPDSQLEDM GYCVFNKYTV PLPSPVQDSE NLSGESGSFY
910 920 930 940 950
EGTDDKVRRD LATDLSLIEV KLAAAGRVKD EFTAEKEASP PSSADKSGLS
960 970 980 990 1000
REFDQDRKAN DKLDTVLEKS EEHVDSKEHA KESEEVGDKV ELFGLGVTYE
1010 1020 1030 1040 1050
QTSAKELITT KETAPERAEK GLSSVPEVAE VETTTKADQG LDVAAKKDDQ
1060 1070 1080 1090 1100
SPLDIKVSDF GQMASGMSVD AGKTIELKFE VDQQLTLSSE APQETDSFMG
1110 1120 1130 1140 1150
IESSHVKDGA KVSETEVKEK VAKPDLVHQE AVDKEESYES SGEHESLTME
1160 1170 1180 1190 1200
SLKPDEGKKE TSPETSLIQD EVALKLSVEI PCPPPVSEAD SSIDEKAEVQ
1210 1220 1230 1240 1250
MEFIQLPKEE STETPDIPAI PSDVTQPQPE AVVSEPAEVR GEEEEIEAEG
1260 1270 1280 1290 1300
EYDKLLFRSD TLQITDLLVP GSREEFVETC PGEHKGVVES VVTIEDDFIT
1310 1320 1330 1340 1350
VVQTTTDEGE LGSHSVRFAA PVQPEEERRP YPHDEELEVL MAAEAQAEPK
1360 1370 1380 1390 1400
DGSPDAPATP EKEEVPFSEY KTETYDDYKD ETTIDDSIMD ADSLWVDTQD
1410 1420 1430 1440 1450
DDRSILTEQL ETIPKEERAE KEARRPSLEK HRKEKPFKTG RGRISTPERR
1460 1470 1480 1490 1500
EVAKKEPSTV SRDEVRRKKA VYKKAELAKE SEVQAHSPSR KLILKPAIKY
1510 1520 1530 1540 1550
TRPTHLSCVK RKTTATSGES AQAPSAFKQA KDKVTDGITK SPEKRSSLPR
1560 1570 1580 1590 1600
PSSILPPRRG VSGDREENSF SLNSSISSAR RTTRSEPIRR AGKSGTSTPT
1610 1620 1630 1640 1650
TPGSTAITPG TPPSYSSRTP GTPGTPSYPR TPGTPKSGIL VPSEKKVAII
1660 1670 1680 1690 1700
RTPPKSPATP KQLRLINQPL PDLKNVKSKI GSTDNIKYQP KGGQVRILNK
1710 1720 1730 1740 1750
KMDFSKVQSR CGSKDNIKHS AGGGNVQIVT KKIDLSHVTS KCGSLKNIRH
1760 1770 1780 1790 1800
RPGGGRVKIE SVKLDFKEKA QAKVGSLDNA HHVPGGGNVK IDSQKLNFRE
1810 1820 1830 1840 1850
HAKARVDHGA EIITQSPSRS SVASPRRLSN VSSSGSINLL ESPQLATLAE
1860
DVTAALAKQG L
Length:1,861
Mass (Da):202,411
Last modified:June 1, 1994 - v3
Checksum:i42DCF116D21EF54E
GO
Isoform 2 (identifier: P15146-2) [UniParc]FASTAAdd to Basket

Also known as: MAP2b

The sequence of this isoform differs from the canonical sequence as follows:
     1695-1725: Missing.

Show »
Length:1,830
Mass (Da):199,053
Checksum:i2A52B720268B0551
GO
Isoform 3 (identifier: P15146-3) [UniParc]FASTAAdd to Basket

Also known as: MAP2c

The sequence of this isoform differs from the canonical sequence as follows:
     152-1514: Missing.
     1695-1725: Missing.

Note: Contains a phosphoserine at position 319. Contains a phosphoserine at position 350. Contains a phosphoserine at position 382.

Show »
Length:467
Mass (Da):49,300
Checksum:i35ACB5CA6E542606
GO
Isoform 4 (identifier: P15146-4) [UniParc]FASTAAdd to Basket

Also known as: MAP2d

The sequence of this isoform differs from the canonical sequence as follows:
     152-1514: Missing.

Show »
Length:498
Mass (Da):52,658
Checksum:iD0CE2BD6CE0ABA3E
GO

Alternative sequence

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Alternative sequencei152 – 15141363Missing in isoform 3 and isoform 4. 1 PublicationVSP_003198Add
BLAST
Alternative sequencei1695 – 172531Missing in isoform 2 and isoform 3. 3 PublicationsVSP_003199Add
BLAST

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X51842 mRNA. Translation: CAA36135.1.
X17682 mRNA. Translation: CAA35667.1.
X71487 mRNA. Translation: CAA50588.1.
PIRiA37981.
I55502. S33176.
RefSeqiXP_008765430.1. XM_008767208.1. [P15146-3]
UniGeneiRn.10484.
Rn.136301.
Rn.230443.

Genome annotation databases

GeneIDi25595.
KEGGirno:25595.
UCSCiRGD:3044. rat. [P15146-1]

Keywords - Coding sequence diversityi

Alternative splicing

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X51842 mRNA. Translation: CAA36135.1 .
X17682 mRNA. Translation: CAA35667.1 .
X71487 mRNA. Translation: CAA50588.1 .
PIRi A37981.
I55502. S33176.
RefSeqi XP_008765430.1. XM_008767208.1. [P15146-3 ]
UniGenei Rn.10484.
Rn.136301.
Rn.230443.

3D structure databases

DisProti DP00122.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

IntActi P15146. 4 interactions.
MINTi MINT-261227.

PTM databases

PhosphoSitei P15146.

Proteomic databases

PaxDbi P15146.
PRIDEi P15146.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

GeneIDi 25595.
KEGGi rno:25595.
UCSCi RGD:3044. rat. [P15146-1 ]

Organism-specific databases

CTDi 4133.
RGDi 3044. Map2.

Phylogenomic databases

eggNOGi NOG148882.
HOGENOMi HOG000113477.
HOVERGENi HBG006322.
InParanoidi P15146.
KOi K10430.
PhylomeDBi P15146.

Gene expression databases

Genevestigatori P15146.

Family and domain databases

InterProi IPR027324. MAP2/MAP4/Tau.
IPR013588. MAP2_projctn.
IPR001084. MAP_tubulin-bd_rpt.
[Graphical view ]
PANTHERi PTHR11501. PTHR11501. 1 hit.
Pfami PF08377. MAP2_projctn. 1 hit.
PF00418. Tubulin-binding. 4 hits.
[Graphical view ]
PROSITEi PS00229. TAU_MAP_1. 3 hits.
PS51491. TAU_MAP_2. 4 hits.
[Graphical view ]
ProtoNeti Search...

Publicationsi

  1. "Complete cDNA sequence encoding rat high and low molecular weight MAP2."
    Kindler S., Schwanke B., Schulz B., Garner C.C.
    Nucleic Acids Res. 18:2822-2822(1990) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 2).
    Strain: Wistar.
    Tissue: Brain.
  2. "Molecular structure of microtubule-associated protein 2b and 2c from rat brain."
    Kindler S., Schulz B., Goedert M., Garner C.C.
    J. Biol. Chem. 265:19679-19684(1990) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 2).
    Strain: Wistar.
    Tissue: Brain.
  3. "Nucleotide and amino acid sequences of embryonic rat MAP2c."
    Doll T., Papandrikopoulou A., Matus A.
    Nucleic Acids Res. 18:361-361(1990) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 3).
  4. "Embryonic MAP2 lacks the cross-linking sidearm sequences and dendritic targeting signal of adult MAP2."
    Papandrikopoulou A., Doll T., Tucker R.P., Garner C.C., Matus A.
    Nature 340:650-652(1989) [PubMed] [Europe PMC] [Abstract]
    Cited for: DISCUSSION OF SEQUENCE.
  5. "An isoform of microtubule-associated protein 2 (MAP2) containing four repeats of the tubulin-binding motif."
    Doll T., Meichsner M., Riederer B.M., Honegger P., Matus A.
    J. Cell Sci. 106:633-640(1993) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA] OF 1695-1725 (ISOFORM 1).
  6. "MARK - a novel family of protein kinases that phosphorylate microtubule-associated proteins and trigger microtubule disruption."
    Drewes G., Ebneth A., Preuss U., Mandelkow E.-M., Mandelkow E.
    Cell 89:297-308(1997) [PubMed] [Europe PMC] [Abstract]
    Cited for: PHOSPHORYLATION AT SER-1682.
    Strain: Sprague-Dawley.
    Tissue: Brain.
  7. "Phosphorylation-dependent localization of microtubule-associated protein MAP2c to the actin cytoskeleton."
    Ozer R.S., Halpain S.
    Mol. Biol. Cell 11:3573-3587(2000) [PubMed] [Europe PMC] [Abstract]
    Cited for: PHOSPHORYLATION AT SER-319; SER-350 AND SER-382 (ISOFORM 3).

Entry informationi

Entry nameiMTAP2_RAT
AccessioniPrimary (citable) accession number: P15146
Entry historyi
Integrated into UniProtKB/Swiss-Prot: April 1, 1990
Last sequence update: June 1, 1994
Last modified: November 26, 2014
This is version 131 of the entry and version 3 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3